Kif1a (BC062891) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	TurboGFP
Synonyms	ATSV; C630002N23Rik; Gm1626; Kns1
Vector	pCMV6-AC-GFP
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MG212065 representing BC062891 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCGGGGCCTCTGTGAAGGTGGCGGTGCGTGTCCGCCCCTTCAACTCCCGGGAAATGAGCCGAGACT CCAAGTGCATCATTCAGATGTCTGGAAGCACCACCACTATTGTCAACCCCAAACAGCCCAAGGAGACACC CAAGAGCTTCAGCTTTGATTACTCCTACTGGTCACATACCTCGCCGGAGGACATCAACTATGCCTCTCAG AAGCAGGTCTACAGGGACATTGGGGAAGAGATGCTACAGCATGCCTTTGAGGGCTACAATGTGTGCATCT TTGCCTATGGGCAGACGGGGGCCGGCAAGTCCTACACCATGATGGGGAAACAGGAGAAGGACCAGCAAGG CATCATTCCACAGCTCTGCGAAGACCTCTTCTCTCGAATCAACGACACCACCAACGACAACATGTCCTAC TCTGTGGAGGTCAGCTACATGGAGATTTACTGTGAGCGAGTCCGTGACCTCCTGAACCCCAAGAACAAAG GCAACCTGCGTGTGAGGGAGCACCCTCTGCTGGGGCCTTATGTGGAGGACCTCTCCAAGCTGGCTGTAAC CTCCTACAATGACATCCAGGACCTCATGGACTCAGGGAACAAGGCCAGGACTGTGGCGGCCACAAACATG AATGAGACCAGCAGCCGTTCTCACGCAGTCTTCAACATCATCTTTACCCAGAAGCGCCATGATGCAGAGA CCAACATCACCACAGAGAAAGTGAGCAAAATCAGCCTGGTGGACCTGGCAGGCAGTGAACGAGCCGACTC CACAGGAGCCAAGGGCACGCGACTCAAGGAGGGGGCCAACATCAACAAGTCTTTGACCACCCTGGGAAAG GTCATCTCTGCTCTGGCTGAAATGGACTCTGGACCCAACAAGAACAAGAAAAAGAAGAAGACAGATTTCA TCCCTTACCGAGACTCCGTATTGACATGGCTTCTCCGGGAAAACCTCGGTGGCAACTCAAGGACAGCCAT GGTGGCAGCCCTGAGCCCTGCGGACATCAACTATGATGAGACCCTCAGCACACTGAGATATGCAGATCGG GCCAAACAAATCCGCTGCAATGCCATCATCAATGAAGACCCCAACAACAAGCTGATTAGAGAACTGAAGG ATGAGGTGACCAGGCTGAGAGACCTGCTGTATGCCCAAGGTCTTGGCGATATCACTGACATGACCAATGC CCTGGTGGGCATGAGCCCCTCATCTTCACTCTCAGCCCTGTCCAGCCGTGCAGCCTCTGTGTCCAGTCTC CATGAGCGCATCTTGTTTGCTCCAGGCAGCGAGGAGGCTATTGAAAGGCTGAAGGAAACAGAGAAGATCA TTGCTGAACTCAATGAGACCTGGGAGGAGAAGCTGCGGCGGACAGAAGCCATCCGGATGGAGAGGGAAGC CTTGCTGGCTGAGATGGGTGTGGCCATGAGGGAAGATGGTGGTACCTTGGGTGTGTTTTCTCCCAAAAAG ACTCCTCATCTTGTCAACCTGAATGAGGACCCTTTGATGTCCGAATGCCTCCTTTACTACATCAAAGATG GCGTCACCAGAGTGGGCAGAGAAGATGCAGAGAGGCGGCAAGACATTGTCCTGAGTGGACACTTCATCAA AGAGGAGCACTGCATCTTCCGGAGTGACTCCCGAGGAGGCGGAGAAGCTGTGGTGACCCTGGAGCCCTGT GAAGGAGCAGACACATATGTCAACGGCAAGAAAGTCACTGAGCCCAGCATCCTACGGTCAGGAAACCGCA TCATCATGGGAAAAAGCCACGTGTTCCGGTTTAACCACCCTGAGCAGGCCCGTCAGGAGCGTGAGCGAAC ACCTTGTGCAGAGACACCTGCTGAGCCAGTGGACTGGGCCTTTGCCCAGCGGGAACTGCTGGAGAAGCAA GGCATTGACATGAAGCAGGAGATGGAGCAGAGGCTCCAGGAGTTGGAGGACCAGTACCGGAGGGAGCGGG AGGAGGCCACATACCTTCTGGAGCAGCAGAGGCTGGACTATGAGAGTAAGCTGGAGGCCCTACAGAAACA GATGGACTCCAGGTACTACCCAGAGGTGAACGAGGAGGAAGAAGAGCCAGAGGATGAAGTCCAATGGACA GAACGAGAATGTGAGCTGGCATTGTGGGCCTTCCGGAAGTGGAAGTGGTACCAGTTTACTTCTCTGCGGG ACCTGCTGTGGGGCAATGCCATCTTCCTGAAGGAGGCCAACGCCATCAGTGTGGAGCTAAAGAAGAAGGT CCAGTTCCAGTTTGTCCTCCTCACGGACACCCTGTACTCCCCACTGCCGCCAGACCTACTGCCCCCAGAA GCCGCCAAAGACCGAGAGACACGACCCTTCCCCAGGACCATTGTGGCAGTGGAGGTCCAGGACCAGAAGA ATGGGGCCACCCACTACTGGACACTGGAGAAACTCAGGCAACGCCTGGACCTGATGCGGGAAATGTACGA CAGGGCTGCAGAGGTTCCCTCCAGCGTCGTTGAGGACTGTGACAACGTGGTGACCGGCGGGGACCCCTTC TATGACCGCTTCCCCTGGTTCCGACTGGTGGGCAGGGCCTTCGTATACCTGAGCAACCTGCTGTACCCTG TGCCCCTGGTGCACCGAGTGGCAATCGTCAGCGAAAAGGGAGAGGTGAAAGGCTTCCTCCGTGTGGCGGT GCAGGCCATCTCAGCTGATGAAGAGGCTCCGGATTATGGCTCGGGTGTCCGTCAGTCAGGAACTGCTAAA ATCTCCTTTGATGACCAGCATTTTGAAAAGTTCCAATCTGAGTCCTGCCCTGTGGTGGGGATGTCCCGCT CAGGAACTTCCCAAGAGGAGCTGCGCATTGTAGAAGGCCAGGGTCAGGGGGCTGATGCTGGGCCTTCAGC TGATGAAGTTAACAACAACACCTGTTCAGCTGTGCCTCCTGAAGGCCTCATGGATAGCCCAGAGAAGGCT GCCCTGGATGGGCCTCTGGACACAGCCCTGGACCACCTCCGTCTAGGCAGTACCTTCACCTTCCGGGTGA CAGTCCTGCAGGCGTCCAGTATCTCTGCTGAATACGCTGACATCTTCTGCCAGTTCAACTTTATCCACCG CCACGATGAGGCCTTCTCCACTGAACCCCTGAAGAACACTGGCAGAGGACCCCCTCTTGGCTTCTACCAT GTCCAAAATATTGCGGTAGAGGTGACCAAGTCCTTCATCGAATACATCAAGAGCCAGCCCATTGTGTTCG AGGTCTTTGGGCACTACCAGCAGCACCCGTTCCCACCTCTTTGCAAGGATGTCCTCAGCCCCCTGAGACC CTCACGTCGCCACTTCCCCAGGGTCATGCCGTTATCCAAGCCAGTGCCCGCCACTAAGCTCAGCACGATG ACACGGCCTTCCCCAGGTCCCTGCCATTGCAAGTATGACCTGCTGGTCTACTTCGAGATCTGCGAGCTGG AAGCCAATGGCGATTACATCCCTGCTGTGGTGGACCACCGTGGTGGCATGCCGTGCATGGGGACTTTCCT TCTCCATCAGGGCATCCAGAGACGGATAACTGTGACACTGTTACATGAGACAGGAAGCCACATCCGCTGG AAGGAAGTGCGCGAGCTAGTTGTGGGTCGCATCCGAAACACCCCAGAAACTGACGAAGCTCTGATTGACC CCAACATCTTGTCCCTCAACATCCTCTCATCAGGATACGTCCATCCAGCCCAGGATGACCGGACCTTCTA CCAGTTTGAAGCAGCCTGGGACAGTTCCATGCATAACTCTCTCCTGCTGAATCGTGTCACCCCTTACCGG GAGAAAATCTACATGACGCTCTCTGCCTACATTGAGATGGAGAACTGTACCCAGCTAGCTGTCATCACCA AGGACTTCTGCATGGTTTTCTACTCTCGGGACGCCAAGCTACCCGCCTCACGCTCCATTCGCAACCTGTT TGGCAGTGGCAGCCTGCGGGCCACAGAAGGCAACCGTGTGACTGGTGTGTATGAGCTCAGCCTTTGCCAC GTGGCCGACGCAGGCAGCCCAGGGATGCAGCGTCGGCGCCGGCGGGTGCTGGACACATCAGTAGCCTATG TCCGGGGTGAGGAGAACCTGGCTGGCTGGAGGCCCCGAAGTGACAGCCTGATCCTAGATCACCAGTGGGA ACTGGAGAAACTGAGCCTCTTACAGGAGGTGGAAAAGACCAGGCACTACCTGCTCCTTCGGGAGAAGCTG GAGACGACCCAGCGCCCTGGCCCTGAGGTGCTGTCCCCAGCCTCCAGTGAGGACTCTGAGTCCCGTAGTT CTTCAGGTGCCTCTTCCCCATTGTCAGCCGAGGGTCAGCCGTCACCTCTGGAGGCTCCCAACGAGAGGCA GAGGGAGCTGGCTGTCAAGTGCTTACGACTCCTCATGCACACATTCAACAGGGAATACACCCACAGCCAC GTCTGTATCAGCGCCAGCGAGAGCAAGCTCTCTGAGATGTCTGTCACCCTGATGCGGGACCCATCCATGT CCCCTCTGGGTGCTGCTACACTCACTCCCTCCTCCACTTGCCCCTCTCTGATCGAAGGACGATACGGTGC CACTGACGTGAGGACCCCCCAGCCCTGCTCCCGGCCCGCCAGCCCAGAGCCCGAGCTCCTGCCAGAACTT GACTCTAAGAAGACTCCCTCCCCTGTTCGGGCAACAGAGACAGAGAAGGAGCCCCAGCGACTGCTGGTCC CTGACATCCAAGAGATCCGAGTCAGCCCAATCGTCTCCAAGAAGGGATACCTGCATTTCCTGGAGCCACA CACGGCTGGCTGGGCCAAGCGCTTTGTGGTGGTGAGGCGTCCCTACGCCTACATGTACAACAGTGACAAG GACACGGTGGAGAGATTTGTGCTCAACCTCTCCACTGCGCAGGTGGAGTACAGCGAGGACCAGCAGGCCA TGCTCAAGACACCCAACACATTCGCTGTGTGCACGGAGCACCGAGGCATCCTGCTGCAGGCCAACAGTGA CAAGGACATGCACGACTGGCTGTATGCCTTCAACCCGCTCCTGGCTGGGACCATCCGGTCCAAGCTCTCT CGAAGGAGATCTGCGCAGATGCGGGTC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA Protein Sequence (showhide) >MG212065 representing BC062891 Red=Cloning site Green=Tags(s) MAGASVKVAVRVRPFNSREMSRDSKCIIQMSGSTTTIVNPKQPKETPKSFSFDYSYWSHTSPEDINYASQ KQVYRDIGEEMLQHAFEGYNVCIFAYGQTGAGKSYTMMGKQEKDQQGIIPQLCEDLFSRINDTTNDNMSY SVEVSYMEIYCERVRDLLNPKNKGNLRVREHPLLGPYVEDLSKLAVTSYNDIQDLMDSGNKARTVAATNM NETSSRSHAVFNIIFTQKRHDAETNITTEKVSKISLVDLAGSERADSTGAKGTRLKEGANINKSLTTLGK VISALAEMDSGPNKNKKKKKTDFIPYRDSVLTWLLRENLGGNSRTAMVAALSPADINYDETLSTLRYADR AKQIRCNAIINEDPNNKLIRELKDEVTRLRDLLYAQGLGDITDMTNALVGMSPSSSLSALSSRAASVSSL HERILFAPGSEEAIERLKETEKIIAELNETWEEKLRRTEAIRMEREALLAEMGVAMREDGGTLGVFSPKK TPHLVNLNEDPLMSECLLYYIKDGVTRVGREDAERRQDIVLSGHFIKEEHCIFRSDSRGGGEAVVTLEPC EGADTYVNGKKVTEPSILRSGNRIIMGKSHVFRFNHPEQARQERERTPCAETPAEPVDWAFAQRELLEKQ GIDMKQEMEQRLQELEDQYRREREEATYLLEQQRLDYESKLEALQKQMDSRYYPEVNEEEEEPEDEVQWT ERECELALWAFRKWKWYQFTSLRDLLWGNAIFLKEANAISVELKKKVQFQFVLLTDTLYSPLPPDLLPPE AAKDRETRPFPRTIVAVEVQDQKNGATHYWTLEKLRQRLDLMREMYDRAAEVPSSVVEDCDNVVTGGDPF YDRFPWFRLVGRAFVYLSNLLYPVPLVHRVAIVSEKGEVKGFLRVAVQAISADEEAPDYGSGVRQSGTAK ISFDDQHFEKFQSESCPVVGMSRSGTSQEELRIVEGQGQGADAGPSADEVNNNTCSAVPPEGLMDSPEKA ALDGPLDTALDHLRLGSTFTFRVTVLQASSISAEYADIFCQFNFIHRHDEAFSTEPLKNTGRGPPLGFYH VQNIAVEVTKSFIEYIKSQPIVFEVFGHYQQHPFPPLCKDVLSPLRPSRRHFPRVMPLSKPVPATKLSTM TRPSPGPCHCKYDLLVYFEICELEANGDYIPAVVDHRGGMPCMGTFLLHQGIQRRITVTLLHETGSHIRW KEVRELVVGRIRNTPETDEALIDPNILSLNILSSGYVHPAQDDRTFYQFEAAWDSSMHNSLLLNRVTPYR EKIYMTLSAYIEMENCTQLAVITKDFCMVFYSRDAKLPASRSIRNLFGSGSLRATEGNRVTGVYELSLCH VADAGSPGMQRRRRRVLDTSVAYVRGEENLAGWRPRSDSLILDHQWELEKLSLLQEVEKTRHYLLLREKL ETTQRPGPEVLSPASSEDSESRSSSGASSPLSAEGQPSPLEAPNERQRELAVKCLRLLMHTFNREYTHSH VCISASESKLSEMSVTLMRDPSMSPLGAATLTPSSTCPSLIEGRYGATDVRTPQPCSRPASPEPELLPEL DSKKTPSPVRATETEKEPQRLLVPDIQEIRVSPIVSKKGYLHFLEPHTAGWAKRFVVVRRPYAYMYNSDK DTVERFVLNLSTAQVEYSEDQQAMLKTPNTFAVCTEHRGILLQANSDKDMHDWLYAFNPLLAGTIRSKLS RRRSAQMRV TRTRPLE - GFP Tag - V
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene
ACCN	BC062891
ORF Size	5067 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC062891, AAH62891
RefSeq Size	5473 bp
RefSeq ORF	5069 bp
Locus ID	16560

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