Myom1 (BC060378) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	TurboGFP
Synonyms	D430047A17Rik
Vector	pCMV6-AC-GFP
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MG212037 representing BC060378 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCTCTGCCCTTTTATCAGCGGTCCCATCAGCACTATGATCTCAGTTACCGGAACAAGGACCTTCGCA CGACCATGAGCCACTACCAACAGGAGAAGAAGCGCTCTGCTGTCTACACCCATGGCTCCACTGCCTACAG CAGCCGCTCCTTGGCAGCACGCCGCCAGGAGTCAGAGGCCTTCAGTCAAGCATCAGCCACCTCTTACCAG CAGCAGGCCTCACAGACCTACAGTCTTGGAGCATCATCATCATCCCGGCATTCTCAAGGGTCTGAAGTCA GCCGGAAGACAGCCTCTGCATATGATTACGGCTATTCCCACGGACTCACAGATTCCAGTCTGCTATTAGA AGATTATTCATCCAAGTTGAGCCCCCAAACAAAGAGAGCCAAGCGGAGCCTTCTGTCTGGAGAGGAGACT GGAAGCTTGCCAGGCAACTACCTGGTGCCTATCTACTCTGGACGGCAAGTGCACATCAGTGGGATCAGAG ACTCGGAAGAAGAAAGAATTAAAGAGGCTGCTGCTTATATCGCTCAGAAGACTCTCCTTGCGAGTGAGGA AGCTATTGCAGCTTCCAAACAGAGCACAGCCTCCAAACAGTCCGCAACCTCCAAACGGACCACGTCCACC CTTCAACGAGAGGAAACGTTTGAAAAGAAGTCGAGGAACATTGCAATTCGAGAAAAGGCGGAAGAGCTGT CACTGAAGAAAACATTAGAAGAGACCCAAACATATCACGGCAAGCTAAATGAAGACCATCTCCTTCATGC TCCTGAGTTTATCATTAAGCCTCGTTCTCACACAGTTTGGGAGAAGGAGAATGTGAAGTTGCACTGCTCT GTAGCAGGGTGGCCAGAGCCTCGGCTCACGTGGTATAAGAACCAGGTGCCTATAAATGTCCATGCAAACC CGGGAAAGTACATCATTGAAAGCCGATACGGAATGCATACTCTTGAGATCAGCAAATGTGACTTTGAAGA CACAGCTCAGTACCGGGCCTCGGCGATGAATGTTCAAGGAGAGCTGTCAGCATATGCCTCAGTCGTAGTA AAGAGATATAAGGGAGAACTGGATGAGTCTCTCCTCCGTGGTGGGGTTTCCATGCCTCTCAGCTTTGCTG TGACCCCTTACGGTTATGCATCCAAGTTTGAGATCCACTTCGATGACAAGTTTGATGTGTCCTTTGGGAG AGAAGGAGAGACCATGAGTCTGGGCTGTCGCGTAGTCATCACTCCTGAGATAAAGCACTTTCAGCCCGAG GTCCAGTGGTATAGAAATGGAGCACCTGTTTCTCCATCAAAATGGGTGCAGCCACACTGGAGTGGAGACC GGGCGACACTTACGTTCTCTCACCTCAACAAAGAAGATGAAGGTCTCTACACGATCCGAGTGCGAATGGG AGAGTATTACGAACAGTACAGTGCTTACGTCTTTGTTCGAGATGCTGATGCAGAGATCGAAGGAGCCCCA GCTGCACCCTTGGATGTGGTGTCTTTGGATGCTAACAAGGATTACATCATCATCTCTTGGAAGCAGCCAG CTGTGGATGGAGGGAGCCCTATCCTGGGATACTTTATCGATAAGTGTGAGGTGGGCACAGATACCTGGTC TCAGTGCAATGACACACCTGTGAAGTTTGCTCGGTTTCCAGTCACTGGCTTGATAGAAGGCCGTTCCTAC ATATTCAGAGTCCGAGCTGTGAATAAAACTGGAATAGGCCTGCCATCCCGAGTTTCTGAGCCTGTGGCGG CTTTGGATCCAGCTGAGAAAGCTAGACTAAAAAGTCATCCTTCAGCACCCTGGACTGGACAGATCATTGT CACGGAAGAAGAGCCTACAGAGGGTGTCATTCCTGGCCCCCCCACAGACCTCTCTGTCACCGAGGCCACC CGGAGCTACGTAGTGCTGAGCTGGAAACCCCCTGGGCAGCGAGGCCATGAGGGCATTATGTATTTTGTAG AAAAGTGTGATGTGGGAGCAGAAAACTGGCAGCGGGTCAACACAGAACTCCCGGTGAAGTCTCCTCGCTT TGCTCTGTTTGACCTGGTGGAGGGGAAGTCTTACCGCTTCCGTGTCCGCTGCTCAAATTCAGCAGGAGTT GGTGAGCCCTCAGAAACGACTGAAGTGACCGTCGTAGGGGACAAACTTGATATCCCAAAGGCCCCTGGCA AAATCATCCCAAGTAGAAATACAGATACCTCAGTGGTGGTATCTTGGGAGGAGTCCAGAGACGCCAAGGA ACTGGTTGGGTACTACATCGAGGCCAGTGTCGTTGGCTCTGGCAAGTGGGAACCCTGCAATAACAACCCG GTGAAGGGCTCACGATTTACTTGTCATGGACTGACGACTGCTCAGAGCTATATTTTCCGGGTCAGAGCAG TGAATGCAGCTGGACTTAGCGAATATTCACAGGATTCGGAAGCTATTGAAGTCAAAGCTGCTATCGCCGT GCCATCTGCGCCCTATGACATCACTTGTCTTGAAAGTTTTCGGGACTCCATGGTTCTTGGATGGAAGCAG CCAGATACGACCGGAGGGGCAGAGATTACAGGCTATTATGTGAACTATCGTGAGGTGGTTGGTGAGGTAC CAGGAAAATGGAGGGAAGCTAACATCAAGGCCGTCAGCGATGCAGCGTACAAGATTAGCAACTTGAAGGA AAACACACTGTACCAGTTCCAAGTGTCAGCAATGAACATTGCAGGGCTGGGAGCGCCCTCCACGGTGAGC GAGTGCTTCAAGTGTGAAGAGTGGACCATTGCTGTTCCAGGACCACCACACAGCGTGAAGCTCAGTGAAG TCAGGAAGAACTCCCTGGTTCTCCAGTGGAAGCCTCCAGTCTACTCAGGCCGGACTCCAGTCACGGGTTA CTTTGTGGACCTGAAGGAAGCCAGTGCCAAAGATGACCAATGGCGAGGACTTAATGAGGCAGCCATTGTG AACAAGTACCTGAGGGTGCAAGGCCTCAAAGAGGGTACCAGCTACGTGTTCCGTGTACGTGCTGTCAACC AGGCAGGCGTTGGAAAGCCTTCAGACCTTGCTGGACCTGTTGTGGCTGAAACACGTCCAGGCACCAAAGA GGTTGTGGTGAGTGTGGATGATGACGGAGTCATTTCCTTGAACTTTGAATGTGATCAGATGACTCCCAAG TCAGAATTCGTCTGGTCCAAAGATTATGTACCTACTGAAGACTCTCCACGATTAGAAGTCGAAAACAAAG GCGATAAGACAAAAATGACCTTTAAAGACCTCGGGACAGATGATTTGGGCACCTATTCTTGTGATGTGAC AGACACGGATGGGATAGCGTCAAGCTACCTGATAGACGAGGAGGAAATGAAACGTCTGCTTGCCCTCAGC CAGGAGCACAAGTTCCCGACTGTCCCAACTAAGTCTGAGTTGGCAGTTGAAATTTTGGAGAAAGGTCAGG TCCGGTTTTGGATGCAGGCTGAGAAGCTGTCTAGCAATGCCAAAGTCAGCTACATATTTAACGAGAAGGA AATTTTCGAGGGGCCGAAATACAAGATGCACATAGACCGAAACACGGGCATCATTGAAATGTTCATGGAG AAGCTACAGGACGAGGATGAAGGGACGTACACATTCCAAATTCAAGATGGAAAAGCAACTGGCCATTCGA CTCTTGTTCTCATTGGAGACGTTTATAAGAAGCTACAGAAAGAAGCTGAATTCCAGCGACAAGAATGGAT CAGGAAGCAAGGCCCACATTTCGCTGAGTATTTGAGCTGGGAAGTGACTGGTGAATGTAACGTGCTGTTG AAATGCAAGGTGGCAAATATTAAAAAAGAGACTCACATTGTATGGTACAAAGATGAACGGGAGATCTCCG TGGACGAGAAACATGACTTTAAGGATGGCATTTGTACCCTGCTCATCACAGAGTTTTCCAAGAAAGACGC TGGATTTTATGAAGTTATCCTGAAAGATGACCGAGGAAAAGATAAGAGCAGATTGAAGCTCGTGGATGAA GCCTTCCAAGACTTGATGACTGAAGTATGCAAGAAGATAGCGTTGTCTGCCACAGACCTGAAAATCCAGA GCACAGCGGAGGGCATTCGGCTATACTCCTTTGTCTGTTATTACCTGGATGACTTGAAAGTTAACTGGTC CCACAATGGGACGGGGATTAAGTACACAGACAGAGTCAAGAGTGGCGTGACTGGGGAGCAGATCTGGCTG CAGATCAACGAGCCCACTCCGAATGATAAAGGGAAATACGTGATGGAGCTCTTTGACGGCAAGACTGGAC ACCAGAAGACGGTGGATCTCTCTGGACAAGCATTTGATGAGGCCTTTGCTGAATTCCAGAGGTTAAAACA AGCTGCCATTGCTGAAAAAAATCGGGCCCGGGTGTTAGGTGGTCTCCCTGATGTTGTCACCATTCAAGAG GGCAAGGCACTCAATCTCACTTGCAATGTGTGGGGTGACCCGCCCCCTGAGGTGTCCTGGCTGAAAAACG AGAAGCCACTGACCTCTGACGACCACTGCAGCCTCAAGTTTGAGGCCGGGAAAACCGCCTTCTTCACCAT CTCAGGCGTGAGCACGGCAGACTCCGGCAAGTACGGGCTGGTGGTGAAGAACAAATATGGCTCGGAGACC AGCGACTTCACCGTTAGTGTGTTCATCCCAGAGGAGGAGTTGAGGAAGGGAGCAATGGAGCCTCCCAAGG GCAACCAGAAGTCCAAG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA Protein Sequence (showhide) >MG212037 representing BC060378 Red=Cloning site Green=Tags(s) MSLPFYQRSHQHYDLSYRNKDLRTTMSHYQQEKKRSAVYTHGSTAYSSRSLAARRQESEAFSQASATSYQ QQASQTYSLGASSSSRHSQGSEVSRKTASAYDYGYSHGLTDSSLLLEDYSSKLSPQTKRAKRSLLSGEET GSLPGNYLVPIYSGRQVHISGIRDSEEERIKEAAAYIAQKTLLASEEAIAASKQSTASKQSATSKRTTST LQREETFEKKSRNIAIREKAEELSLKKTLEETQTYHGKLNEDHLLHAPEFIIKPRSHTVWEKENVKLHCS VAGWPEPRLTWYKNQVPINVHANPGKYIIESRYGMHTLEISKCDFEDTAQYRASAMNVQGELSAYASVVV KRYKGELDESLLRGGVSMPLSFAVTPYGYASKFEIHFDDKFDVSFGREGETMSLGCRVVITPEIKHFQPE VQWYRNGAPVSPSKWVQPHWSGDRATLTFSHLNKEDEGLYTIRVRMGEYYEQYSAYVFVRDADAEIEGAP AAPLDVVSLDANKDYIIISWKQPAVDGGSPILGYFIDKCEVGTDTWSQCNDTPVKFARFPVTGLIEGRSY IFRVRAVNKTGIGLPSRVSEPVAALDPAEKARLKSHPSAPWTGQIIVTEEEPTEGVIPGPPTDLSVTEAT RSYVVLSWKPPGQRGHEGIMYFVEKCDVGAENWQRVNTELPVKSPRFALFDLVEGKSYRFRVRCSNSAGV GEPSETTEVTVVGDKLDIPKAPGKIIPSRNTDTSVVVSWEESRDAKELVGYYIEASVVGSGKWEPCNNNP VKGSRFTCHGLTTAQSYIFRVRAVNAAGLSEYSQDSEAIEVKAAIAVPSAPYDITCLESFRDSMVLGWKQ PDTTGGAEITGYYVNYREVVGEVPGKWREANIKAVSDAAYKISNLKENTLYQFQVSAMNIAGLGAPSTVS ECFKCEEWTIAVPGPPHSVKLSEVRKNSLVLQWKPPVYSGRTPVTGYFVDLKEASAKDDQWRGLNEAAIV NKYLRVQGLKEGTSYVFRVRAVNQAGVGKPSDLAGPVVAETRPGTKEVVVSVDDDGVISLNFECDQMTPK SEFVWSKDYVPTEDSPRLEVENKGDKTKMTFKDLGTDDLGTYSCDVTDTDGIASSYLIDEEEMKRLLALS QEHKFPTVPTKSELAVEILEKGQVRFWMQAEKLSSNAKVSYIFNEKEIFEGPKYKMHIDRNTGIIEMFME KLQDEDEGTYTFQIQDGKATGHSTLVLIGDVYKKLQKEAEFQRQEWIRKQGPHFAEYLSWEVTGECNVLL KCKVANIKKETHIVWYKDEREISVDEKHDFKDGICTLLITEFSKKDAGFYEVILKDDRGKDKSRLKLVDE AFQDLMTEVCKKIALSATDLKIQSTAEGIRLYSFVCYYLDDLKVNWSHNGTGIKYTDRVKSGVTGEQIWL QINEPTPNDKGKYVMELFDGKTGHQKTVDLSGQAFDEAFAEFQRLKQAAIAEKNRARVLGGLPDVVTIQE GKALNLTCNVWGDPPPEVSWLKNEKPLTSDDHCSLKFEAGKTAFFTISGVSTADSGKYGLVVKNKYGSET SDFTVSVFIPEEELRKGAMEPPKGNQKSK TRTRPLE - GFP Tag - V
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	BC060378
ORF Size	4707 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC060378, AAH60378
RefSeq Size	5259 bp
RefSeq ORF	4709 bp
Locus ID	17929
Gene Summary	May link the intermediate filament cytoskeleton to the M-disk of the myofibrils in striated muscle. May also contact myosin filaments. Also binds beta-integrins.[UniProtKB/Swiss-Prot Function]

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