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Kdm6a (NM_009483) Mouse Tagged ORF Clone
CAT＃: MG211981
- TrueORF®
Kdm6a (tGFP-tagged) - Mouse ubiquitously transcribed tetratricopeptide repeat gene, X chromosome (Utx)
ORF Plasmid: DDK
"NM_009483" in other vectors (6)
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CNY 16,216.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | Utx |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG211981 representing NM_009483
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAAATCCTGCGGAGTGTCGCTCGCTACCGCCGCCGCCGCCGCCGCCGCCGCCGCTTTCGGTGATGAGG AAAAGAAAATGGCGGCGGGAAAAGCGAGCGGCGAGAGCGAGGAGGCGTCCCCCAGCCTGACAGCGGAGGA GAGGGAGGCGCTCGGCGGACTGGACAGCCGCCTTTTCGGGTTCGTGAGGTTTCATGAAGATGGCGCCAGG ATGAAGGCCCTGCTGGGCAAGGCTGTTCGCTGCTACGAATCTCTAATCTTAAAAGCTGAAGGGAAAGTGG AGTCTGATTTCTTTTGTCAATTAGGTCACTTCAACCTCTTATTGGAAGATTATCCAAAAGCATTATCTGC ATACCAGAGGTACTACAGTTTACAGTCTGATTACTGGAAGAATGCTGCCTTTTTATATGGTCTTGGTTTG GTCTACTTCCATTACAATGCATTTCAGTGGGCTATTAAAGCATTTCAGGAGGTGCTTTATGTCGATCCCA GCTTTTGTCGAGCCAAGGAAATTCATTTACGACTTGGGCTTATGTTCAAAGTGAACACAGACTATGAGTC TAGTTTAAAGCATTTTCAGTTAGCTTTGGTTGACTGTAATCCCTGCACTTTGTCCAATGCTGAAATTCAG TTTCACATTGCCCACTTATATGAAACCCAGAGGAAGTATCATTCTGCAAAAGAAGCTTATGAGCAACTTT TGCAGACAGAAAACCTTTCTGCACAAGTAAAAGCAACTATTTTACAACAATTAGGTTGGATGCATCACAC TGTGGATCTCCTGGGAGATAAGGCCACCAAGGAAAGTTATGCTATTCAGTATCTCCAGAAGTCCTTGGAA GCAGATCCAAATTCTGGCCAGTCCTGGTATTTCCTTGGAAGGTGCTATTCAAGTATTGGGAAAGTTCAGG ATGCCTTTATATCTTACAGGCAATCTATTGATAAATCAGAAGCAAGTGCAGATACATGGTGTTCAATAGG TGTGCTCTATCAACAGCAAAATCAGCCTATGGATGCTTTGCAAGCTTATATTTGTGCTGTACAATTGGAC CACGGTCATGCTGCAGCCTGGATGGATCTAGGCACTCTCTATGAATCCTGCAACCAACCTCAGGATGCTA TTAAATGCTATTTAAATGCAACTAGAAGCAAAAATTGTAGTAATACCTCTGGACTTGCAGCACGAATTAA GTATTTACAGGCTCAGTTGTGTAACCTTCCACAAGGTAGTCTACAGAATAAAACTAAATTACTTCCTAGT ATTGAGGAGGCATGGAGCCTACCAATCCCCGCAGAGCTTACCTCCAGGCAGGGTGCCATGAACACAGCAC AGCAGAATACTTCTGATAATTGGAGTGGTGGCAATGCACCACCTCCAGTAGAACAACAAACTCATTCATG GTGTTTGACACCACAGAAATTACAGCACTTGGAACAGCTCCGAGCAAACAGAAATAATTTAAATCCAGCA CAGAAACTAATGCTGGAACAGCTGGAAAGTCAGTTTGTCTTAATGCAGCAACACCAAATGAGACAAACAG GAGTTGCACAGGTACGGCCTACTGGAATTCTTAATGGGCCAACAGTTGACTCATCACTGCCTACAAACTC AGTTTCTGGCCAGCAGCCACAGCTTCCTCTGACCAGAATGCCTAGTGTCTCTCAGCCTGGAGTCCACACT GCCTGTCCTAGGCAGACTTTGGCCAATGGACCCTTTTCTGCAGGCCATGTTCCCTGTAGCACATCAAGAA CACTGGGAAGTACAGACACTGTTTTGATAGGCAATAATCATGTAACAGGAAGTGGAAGTAATGGAAACGT GCCTTACCTGCAGCGAAACGCACCCACTCTACCTCATAACCGCACAAACCTGACCAGCAGCACAGAGGAG CCGTGGAAAAACCAACTATCTAACTCCACTCAGGGGCTTCACAAAGGTCCGAGTTCACATTTGGCAGGTC CTAATGGTGAACGACCTCTATCTTCCACTGGGCCTTCCCAGCATCTCCAGGCAGCTGGCTCTGGTATTCA GAATCAGAATGGACATCCCACCCTGCCTAGCAATTCAGTAACACAGGGGGCTGCTCTCAATCACCTCTCC TCTCACACTGCTACCTCAGGTGGACAACAAGGCATTACCTTAACCAAAGAGAGCAAGCCTTCAGGAAACA CATTGACGGTGCCTGAAACAAGCAGGCAAACTGGAGAGACACCTAACAGCACTGCCAGTGTTGAGGGACT TCCTAATCATGTCCATCAGGTGATGGCAGATGCTGTTTGCAGTCCTAGCCATGGAGATTCTAAGTCACCA GGTTTACTAAGTTCAGACAATCCTCAGCTCTCTGCCTTGTTGATGGGAAAAGCTAATAACAATGTGGGTC CTGGAACCTGTGACAAAGTCAATAACATCCACCCAACTGTCCATACAAAGACTGATAATTCTGTTGCCTC TTCACCATCTTCAGCCATTTCCACAGCAACACCTTCTCCTAAGTCCACTGAACAGACAACCACAAACAGT GTTACCAGCCTTAACAGCCCTCACAGTGGGCTGCACACAATTAATGGAGAAGGAATGGAAGAATCTCAGA GCCCCATTAAAACAGATCTGCTTCTAGTTAGCCACAGACCTAGTCCTCAGATCATACCATCAATGTCTGT GTCCATATATCCCAGCTCAGCAGAAGTTCTGAAAGCTTGCAGGAATCTAGGTAAAAACGGCCTGTCTAAT AGTAGCATTCTGTTGGATAAATGTCCGCCTCCAAGACCACCATCCTCACCATACCCTCCCTTGCCAAAGG ACAAGTTGAATCCACCTACACCTAGTATTTATTTGGAAAATAAACGTGATGCTTTCTTTCCTCCATTACA TCAATTTTGTACAAACCCAAACAACCCTGTTACAGTAATACGTGGCCTTGCTGGAGCTCTTAAATTAGAC TTGGGACTTTTCTCTACTAAAACTTTGGTGGAAGCTAACAATGAACATATGGTAGAAGTGAGGACACAGT TGTTACAACCAGCAGATGAAAATTGGGACCCTACTGGAACCAAGAAAATCTGGCACTGTGAAAGTAATAG ATCTCATACTACAATTGCTAAATATGCTCAGTACCAGGCCTCCTCATTCCAAGAATCATTGAGAGAAGAA AATGAGAAAAGAAGTCACCATAAAGACCACTCAGACAGTGAATCTACATCATCAGATAATTCTGGGAAAA GAAGAAAAGGACCCTTTAAAACCATTAAGTTTGGGACCAACATTGACCTGTCTGATGACAAAAAGTGGAA GTTACAGCTACATGAGCTGACTAAACTTCCTGCCTTCGTGAGAGTTGTATCTGCAGGAAATCTTTTAAGC CACGTTGGTCATACTATACTGGGCATGAACACAGTTCAACTATACATGAAAGTTCCAGGAAGCAGAACAC CAGGTCATCAAGAAAATAACAACTTCTGTTCAGTTAATATAAATATTGGCCCAGGTGACTGTGAATGGTT TGTTGTTCCTGAAGGCTACTGGGGTGTTTTGAATGACTTCTGTGAAAAAAATAATTTGAATTTCTTAATG GGTTCTTGGTGGCCCAACCTTGAAGATCTATATGAAGCAAATGTTCCAGTGTATAGGTTTATTCAGCGAC CTGGAGATCTGGTCTGGATAAATGCTGGCACTGTTCATTGGGTTCAAGCTATTGGCTGGTGCAACAATAT TGCTTGGAATGTTGGTCCACTTACAGCCTGTCAGTATAAGTTAGCAGTGGAACGTTATGAATGGAACAAG TTGCAAAATGTAAAGTCAATAGTACCCATGGTTCATCTTTCCTGGAATATGGCACGAAATATCAAGGTTT CAGATCCAAAGCTTTTTGAAATGATTAAGTATTGTCTTCTGAGAACGCTGAAGCAATGTCAGACATTGAG GGAAGCTCTAATTGCTGCAGGAAAAGAGATCATATGGCACGGGCGGACAAAAGAAGAACCAGCTCATTAT TGTAGTATTTGTGAGGTGGAGGTTTTTGATCTGCTTTTTGTCACTAATGAGAGTAATTCTCGAAAAACCT ACATAGTACATTGCCAAGATTGTGCACGAAAAACAAGTGGGAATCTGGAAAATTTTGTGGTGCTAGAACA GTACAAAATGGAGGATCTGATGCAAGTCTATGACCAATTTACATTAGTAAGTGAAATCAACATGCTCCTC CATTACCATCCGCCTCATCTTGATATTGTTCCATGGACATTAAACATGAGACCTTTTCTGCTATTCAGAA AG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG211981 representing NM_009483
Red=Cloning site Green=Tags(s) MKSCGVSLATAAAAAAAAAFGDEEKKMAAGKASGESEEASPSLTAEEREALGGLDSRLFGFVRFHEDGAR MKALLGKAVRCYESLILKAEGKVESDFFCQLGHFNLLLEDYPKALSAYQRYYSLQSDYWKNAAFLYGLGL VYFHYNAFQWAIKAFQEVLYVDPSFCRAKEIHLRLGLMFKVNTDYESSLKHFQLALVDCNPCTLSNAEIQ FHIAHLYETQRKYHSAKEAYEQLLQTENLSAQVKATILQQLGWMHHTVDLLGDKATKESYAIQYLQKSLE ADPNSGQSWYFLGRCYSSIGKVQDAFISYRQSIDKSEASADTWCSIGVLYQQQNQPMDALQAYICAVQLD HGHAAAWMDLGTLYESCNQPQDAIKCYLNATRSKNCSNTSGLAARIKYLQAQLCNLPQGSLQNKTKLLPS IEEAWSLPIPAELTSRQGAMNTAQQNTSDNWSGGNAPPPVEQQTHSWCLTPQKLQHLEQLRANRNNLNPA QKLMLEQLESQFVLMQQHQMRQTGVAQVRPTGILNGPTVDSSLPTNSVSGQQPQLPLTRMPSVSQPGVHT ACPRQTLANGPFSAGHVPCSTSRTLGSTDTVLIGNNHVTGSGSNGNVPYLQRNAPTLPHNRTNLTSSTEE PWKNQLSNSTQGLHKGPSSHLAGPNGERPLSSTGPSQHLQAAGSGIQNQNGHPTLPSNSVTQGAALNHLS SHTATSGGQQGITLTKESKPSGNTLTVPETSRQTGETPNSTASVEGLPNHVHQVMADAVCSPSHGDSKSP GLLSSDNPQLSALLMGKANNNVGPGTCDKVNNIHPTVHTKTDNSVASSPSSAISTATPSPKSTEQTTTNS VTSLNSPHSGLHTINGEGMEESQSPIKTDLLLVSHRPSPQIIPSMSVSIYPSSAEVLKACRNLGKNGLSN SSILLDKCPPPRPPSSPYPPLPKDKLNPPTPSIYLENKRDAFFPPLHQFCTNPNNPVTVIRGLAGALKLD LGLFSTKTLVEANNEHMVEVRTQLLQPADENWDPTGTKKIWHCESNRSHTTIAKYAQYQASSFQESLREE NEKRSHHKDHSDSESTSSDNSGKRRKGPFKTIKFGTNIDLSDDKKWKLQLHELTKLPAFVRVVSAGNLLS HVGHTILGMNTVQLYMKVPGSRTPGHQENNNFCSVNINIGPGDCEWFVVPEGYWGVLNDFCEKNNLNFLM GSWWPNLEDLYEANVPVYRFIQRPGDLVWINAGTVHWVQAIGWCNNIAWNVGPLTACQYKLAVERYEWNK LQNVKSIVPMVHLSWNMARNIKVSDPKLFEMIKYCLLRTLKQCQTLREALIAAGKEIIWHGRTKEEPAHY CSICEVEVFDLLFVTNESNSRKTYIVHCQDCARKTSGNLENFVVLEQYKMEDLMQVYDQFTLVSEINMLL HYHPPHLDIVPWTLNMRPFLLFRK TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_009483 |
| ORF Size | 4272 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_009483.2 |
| RefSeq Size | 5309 bp |
| RefSeq ORF | 4275 bp |
| Locus ID | 22289 |
| UniProt ID | O70546 |
| Gene Summary | Histone demethylase that specifically demethylates 'Lys-27' of histone H3, thereby playing a central role in histone code. Demethylates trimethylated and dimethylated but not monomethylated H3 'Lys-27'. Plays a central role in regulation of posterior development, by regulating HOX gene expression. Demethylation of 'Lys-27' of histone H3 is concomitant with methylation of 'Lys-4' of histone H3, and regulates the recruitment of the PRC1 complex and monoubiquitination of histone H2A (By similarity). Plays a demethylase-independent role in chromatin remodeling to regulate T-box family member-dependent gene expression (PubMed:21095589).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC224431 | Kdm6a (untagged) - Mouse 4lysine (K)-specific demethylase 6A (Kdm6a), (10ug) |
CNY 14,624.00 |
|
| MR211981 | Kdm6a (Myc-DDK-tagged) - Mouse 4lysine (K)-specific demethylase 6A (Kdm6a) |
CNY 14,616.00 |
|
| MR211981L1 | Lenti ORF clone of Kdm6a (Myc-DDK-tagged) - Mouse 4lysine (K)-specific demethylase 6A (Kdm6a) |
CNY 16,150.00 |
|
| MR211981L2 | Lenti ORF clone of Kdm6a (mGFP-tagged) - Mouse 4lysine (K)-specific demethylase 6A (Kdm6a) |
CNY 17,016.00 |
|
| MR211981L3 | Lenti ORF clone of Kdm6a (Myc-DDK-tagged) - Mouse 4lysine (K)-specific demethylase 6A (Kdm6a) |
CNY 16,150.00 |
|
| MR211981L4 | Lenti ORF clone of Kdm6a (mGFP-tagged) - Mouse 4lysine (K)-specific demethylase 6A (Kdm6a) |
CNY 17,016.00 |
