Rreb1 (BC080680) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	TurboGFP
Synonyms	1110037N09Rik; AA414966; B930013M22Rik
Vector	pCMV6-AC-GFP
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MG211895 representing BC080680 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGACGTCGAATTCGCCCATTGGTTTAGAAGGCTCAGACCTGTCTTCCATCAACACCATGATGTCAGCAG TAATGAGCGTAGCGAGTGTCACAGAGAATGGTGGGAGCCCCCAGGGCATCAAGTCCCCCATGAAACCTCC AGGACCAAATCGGATTGGCAGAAGGAACCAGGAAACGAAAGAGGAGAAGTCTTCCTACAACTGTCCCCTA TGTGAAAAGATTTGCACCACCCAGCACCAGCTGACCATGCACATCCGTCAGCACAACACAGACACGGGAG GAGCTGACCACGCATGCAGTATCTGTGGGAAGTCGCTGAGCTCGGCCAGCTCCCTGGATCGTCACATGCT GGTGCACTCTGGCGAGAGGCCTTACAAGTGTACAGTGTGTGGCCAGTCTTTCACCACCAATGGGAACATG CACAGACATATGAAGATTCATGAGAAGGATACCAACAGTACTACAGCTGCAGCCCCTCCATCCCCTCTGA AGCGCAGGCGGTTGTCCTCCAAAAGGAAGCTGAGTCACGATGCCGAGTCAGAAGACCCAGGACCAGCTAA AAAGATGGTAGAAGACGGGCAGTCAGGCGATTTGGACAAGATGAGCGATGAAATCTTTCACTGCCCAGTG TGTTTCAAGGAGTTTGTTTGCAAGTATGAACTGGAGACCCACATGGAGACCCACTCAGATAACCCACTAA GATGTGACATTTGCTGCGTCACCTTCCGCACACATCGAGGACTGCTGCGCCACAATGCACTTGTCCACAA GCAGCTTCCCAGAGATGCCATGGGAAGACCTTTTATCCAGAACAACCCTTCGATTCCTGCTGGCTTCCAT GATTTAGGGTTTACTGACTTCTCCTGTAGGAAGTTTCCTCGAATCTCTCAGGCCTGGTGTGAGACAAACC TACGGCGGTGCATCAGCGAGCAGCACCGGTTTGTGTGTGACACCTGCGACAAGGCGTTCCCCATGCTGTC GTCACTCATCCTGCACAGGCAGAGCCACATCCCTGCCGATCAGGGACGGGAGAAGCTCCAGACCAAGACC CTGGCTGCCGAGTCCTTGGAACAGAAGGCCTTCCTGGCCTTGCTGGGCCTGCAGCACACCAAAGACGTCA AGCCTGCCCCGGCGGAGGAGCTCCTGCCGGACGACAACCAAGCAATACAGCTCCAGACACTTAAGTACCA GCTACCTCAGGAGCCTGGCTGCCCCACCGTGCTGAGTGTGTCTCCTCTTGACGCTGCTTCTTTAGGTGGC TCTCTGACAGTCCTCCCAGCTACCAAGGAGAATATGAAGCATCTGTCCCTGCAGCCCTTCCAGAAGGGCT TCATCATCCAGCCAGACAGTAGCATTGTGGTTAAGCCTATTTCAGGAGAGTCGGCCATTGAGCTGGCAGA TATCCAACAGATTCTAAAGATGGCAGCTTCCGCTCCTCCGCAGATCAGTCTTCCGCCACTTTCCAAGGCC CCTGCCACCCCGCTGCAGGCGATTTTCAAGCACATGCCTCCTTTGAAGCCAAAGCCCTTGGTCACACCCC GGACAGTGGTAGCCGCCTCCACGCCCCCACCTCTCATCAACGCCCAGCAGGCATCTCCCGGTTGTATCAG CCCCAGCCTTCCTCCACAGTCCCTGAAGTTCCTCAAGGGGTCGGTGGAGGCAGTGTCCAATGTTCATCTG CTTCAGTCCAAGTCTGGGATCCAGCCAAGCACCACCACACAGCTCTTCCTGCAGCAAGCTGGAGTGGAGT TGCCGGGCCAGCCCGAGATGAAGACACAGCTGGAACAAGAGAGTATCATTGAGGCCCTGCTGCCCCTCAA CATGGAGGCGAAGATCAAGCAGGAGATAACAGAAGGTGACCTCAAGGCCATCATGACAGGCCCCAGTGGC AAGAAGACCCCCGCCATGCGCAAGGTGCTCTACCCCTGCCGCTTTTGCAACCAGGTGTTTGCTTTCTCTG GAGTCCTGCGAGCCCACGTTCGCTCCCACCTGGGCATCTCACCCTACCAATGCAACATCTGTGACTATAT TGCTGCAGACAAAGCCGCCCTGATCCGTCACATCCGCACACACAGCGGGGAACGGCCTTACATCTGCAAG ATCTGCCACTACCCATTCACGGTCAAAGCCAACTGTGAGCGGCACCTGCGCAAGAAGCACCTCAAGGCCA CCAGGAAGGACATTGAGAAGAACATAGAGTACGTGAGCAGCCCCACCGCAGAGCTGGTGGACGCCTTCTG CGCCCCAGAGACCGTGTGCAGACTGTGCGGGGAGGATCTGAAGCACTACCGAGCTCTACGCATCCACATG CGCACACACTGCAGCCGGGGCCTGGGCGGCTGCCACAAAGGCCGGAAACCCTTCGAGTGCAAGGAATGCA ACGCTCCCTTCGTGGCCAAGCGCAACTGCATCCATCACATCCTCAAGCAGCACCTGCACGTGCCCGAGAA GGACATCGAGAGCTACGTGCTTGCCACCAACAGTGGCCTCGGCCCCGCGGACACGCCCACGGATGCCGCT TCCAGAGGAGAAGAGGGCAGCTGTGTCACTTTCGCGGAGTGCAAGCCCCTCGCCACTTTCCTGGAGCCCC AAAATGGCTTTCTTCACTCGAGCCCCACCCAGCCCCTGCCTTCCCACATCTCTGTCAAGCTGGAGCCAGC CAGCAGCTTTGCCATGGACTTCAACGAACCCCTTGACTTTTCGCAGAAAGGCCTGGCACTGGTCCAAGTG AAGCAGGAAAATGTTTCCTCCTTGCTGACGTCTTCCTCCTCCTCTGCCCTCTATGACTGCTCCATGGAGC CCATTGACCTGTCCATCCCCAAGAGCGTCAAGAAAGGAGACAAGGACACAGTTGTTCCCAGTGACGCCAA GAAACCGGAACCGGAAGCTGGGCAAGCCGAGCCGCTCTCACCCCGCCCACCACCCTGCCCTACCTTGTCA GTGACTGTGGAGCCCAAGGGGAGCCTGGAAACCCCCACAGGCACGGTGGTGGCCGTCACCACAGCTGCCA AGCTAGAGCCCCACACTCAGCCCCTCCAGGGCTCCGTGCAGCTGGCTGTCCCCATCTACTCCCCCGCGCT CGTCAGCAATACTCCCCTCTTGGGCAATTCTGCTGCCCTCTTGAACAACCCAGCCTTGCTTCGGCCCTTA CGGCCAAAGCCCCCCCTCCTCTTGCCAAAGCCCTCGATGACAGAGGAGCTGCCGCCACTGGCCTCCATCG CCCAGATCATTTCTTCCGTGTCCTCGGCCCCTACTCTGCTGAAAACGAAGGTAGCTGACCCTGGACCATC GATCACCAGCAGTAACACTGTGGCCACAGACAGCCCAGGAAGCTCCACCCCCAAAGCTGCCGCCACCCCC ACTGACACCACAAGCTCTAAAGAATCCAGTGAGCCACCCCCTGCAGCCAGCAGCCCTGAGGAAGCCTTGC CTACTGAACAAGGGCCGGCTGCCACTTCCTCGTCGAGGAAGAGGGGCAGGAAAAGGGGACTGAGAAACCG GCCCCTCCCCAACAGCAGTGCTGTGGACCTGGACTCCAGTGGGGAGTTTGCTAGCATCGAGAAGATGCTG GCCACCACAGATACCAACAAGTTCAGTCCCTTTCTGCAGACTGCAGAGGATGACACTCAGGAAGAGGTGG CTGGAGCCCCTGCCGACCAGCATGGGCCCGCTGATGAGGAGCAAGGTAGCCCCGCAGAAGACAGGCTGCT GAGAGCAAAGCGGAACTCCTATGCCAACTGCCTGCAAAAGATCAACTGTCCCCACTGTCCCCGGGTCTTC CCTTGGGCCAGCTCCCTGCAGCGGCACATGCTTACACACACTGGTAAGAAGGCCCTCACGGCTCACCAGG CGGTGAGTCTTGAAAGGAAAGAG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA Protein Sequence (showhide) >MG211895 representing BC080680 Red=Cloning site Green=Tags(s) MTSNSPIGLEGSDLSSINTMMSAVMSVASVTENGGSPQGIKSPMKPPGPNRIGRRNQETKEEKSSYNCPL CEKICTTQHQLTMHIRQHNTDTGGADHACSICGKSLSSASSLDRHMLVHSGERPYKCTVCGQSFTTNGNM HRHMKIHEKDTNSTTAAAPPSPLKRRRLSSKRKLSHDAESEDPGPAKKMVEDGQSGDLDKMSDEIFHCPV CFKEFVCKYELETHMETHSDNPLRCDICCVTFRTHRGLLRHNALVHKQLPRDAMGRPFIQNNPSIPAGFH DLGFTDFSCRKFPRISQAWCETNLRRCISEQHRFVCDTCDKAFPMLSSLILHRQSHIPADQGREKLQTKT LAAESLEQKAFLALLGLQHTKDVKPAPAEELLPDDNQAIQLQTLKYQLPQEPGCPTVLSVSPLDAASLGG SLTVLPATKENMKHLSLQPFQKGFIIQPDSSIVVKPISGESAIELADIQQILKMAASAPPQISLPPLSKA PATPLQAIFKHMPPLKPKPLVTPRTVVAASTPPPLINAQQASPGCISPSLPPQSLKFLKGSVEAVSNVHL LQSKSGIQPSTTTQLFLQQAGVELPGQPEMKTQLEQESIIEALLPLNMEAKIKQEITEGDLKAIMTGPSG KKTPAMRKVLYPCRFCNQVFAFSGVLRAHVRSHLGISPYQCNICDYIAADKAALIRHIRTHSGERPYICK ICHYPFTVKANCERHLRKKHLKATRKDIEKNIEYVSSPTAELVDAFCAPETVCRLCGEDLKHYRALRIHM RTHCSRGLGGCHKGRKPFECKECNAPFVAKRNCIHHILKQHLHVPEKDIESYVLATNSGLGPADTPTDAA SRGEEGSCVTFAECKPLATFLEPQNGFLHSSPTQPLPSHISVKLEPASSFAMDFNEPLDFSQKGLALVQV KQENVSSLLTSSSSSALYDCSMEPIDLSIPKSVKKGDKDTVVPSDAKKPEPEAGQAEPLSPRPPPCPTLS VTVEPKGSLETPTGTVVAVTTAAKLEPHTQPLQGSVQLAVPIYSPALVSNTPLLGNSAALLNNPALLRPL RPKPPLLLPKPSMTEELPPLASIAQIISSVSSAPTLLKTKVADPGPSITSSNTVATDSPGSSTPKAAATP TDTTSSKESSEPPPAASSPEEALPTEQGPAATSSSRKRGRKRGLRNRPLPNSSAVDLDSSGEFASIEKML ATTDTNKFSPFLQTAEDDTQEEVAGAPADQHGPADEEQGSPAEDRLLRAKRNSYANCLQKINCPHCPRVF PWASSLQRHMLTHTGKKALTAHQAVSLERKE TRTRPLE - GFP Tag - V
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	BC080680
ORF Size	3873 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC080680, AAH80680
RefSeq Size	7288 bp
RefSeq ORF	3875 bp
Locus ID	68750
Gene Summary	Transcription factor that binds specifically to the RAS-responsive elements (RRE) of gene promoters. May be involved in Ras/Raf-mediated cell differentiation by enhancing calcitonin expression. Represses the angiotensinogen gene. Negatively regulates the transcriptional activity of AR. Potentiates the transcriptional activity of NEUROD1 (By similarity). Binds specifically to the allelic variant of the CDKN2A promoter present in Balb/c mice, which leads to a down-regulation of CDKN2A expression in this strain, and, as a consequence, to an elevated susceptibility to pristane-induced tumors.[UniProtKB/Swiss-Prot Function]

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