Plcb1 (BC058710) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	TurboGFP
Synonyms	3110043I21Rik; AI132408; mKIAA0581; Plcb
Vector	pCMV6-AC-GFP
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MG211812 representing BC058710 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCTGGGGCTCAGCCCGGAGTGCACGCCTTGCAACTCAAGCCCGTGTGCGTGTCCGACAGCCTCAAGA AGGGCACCAAATTCGTCAAGTGGGATGATGACTCCACTATTGTTACTCCAATTATTTTGAGGACTGATCC TCAGGGATTTTTCTTTTACTGGACAGACCAGAATAAGGAGACGGAGCTGTTAGATCTCAGCCTTGTCAAG GATGCCAGGTGTGGGAAGCACGCCAAAGCTCCCAAGGACCCCAAGTTGCGTGAACTTCTGGATGTTGGGA ACATCGGACACTTGGAACAGCGCATGATAACTGTGGTGTATGGACCAGACCTGGTGAACATTTCCCACTT GAATCTTGTGGCTTTTCAAGAGGAAGTAGCCAAGGAATGGACAAATGAGGTTTTCAGCTTGGCAACAAAC CTGCTGGCTCAGAACATGTCCAGGGACGCATTTCTGGAGAAAGCATATACTAAGCTCAAGCTCCAGGTCA CCCCAGAAGGCCGCATTCCTCTTAAAAACATCTATCGATTGTTCTCAGCAGACCGGAAGCGCGTTGAAAC TGCACTAGAGGCTTGTAGTCTTCCATCTTCGAGGAATGACTCCATTCCCCAAGAGGACTTCACTCCAGAT GTATACAGAGTTTTCCTGAACAATCTCTGTCCCCGACCTGAAATTGATAACATCTTTTCTGAATTTGGTG CCAAAAGCAAACCGTACCTGACGGTTGATCAGATGATGGATTTTATCAACCTTAAGCAGAGAGATCCCCG GCTCAATGAAATCCTTTACCCACCTCTGAAGCAGGAGCAGGTCCAAGTGTTGATTGAGAAATATGAGCCC AACAGCAGCCTCGCCAAGAAAGGGCAGATGTCAGTGGATGGATTCATGCGCTACCTGAGCGGAGAAGAAA ATGGAGTCGTTTCACCTGAGAAACTGGATTTGAACGAAGACATGTCTCAGCCCCTGTCTCACTATTTCAT CAATTCCTCGCACAACACCTACCTCACAGCTGGCCAGTTGGCTGGGAACTCGTCTGTAGAGATGTATCGC CAGGTGCTTCTGTCTGGATGTCGCTGTGTGGAGCTGGACTGCTGGAAGGGAAGGACCGCTGAGGAAGAGC CTGTCATTACCCATGGATTCACCATGACAACAGAAATATCCTTCAAGGAAGTCATAGAAGCCATCGCAGA GTGTGCGTTCAAGACGTCTCCTTTCCCCATCCTCCTTTCCTTTGAGAACCACGTGGATTCCCCGAAGCAA CAAGCCAAGATGGCCGAGTATTGCCGATTAATCTTTGGTGATGCCCTCCTGATGGAACCACTGGAAAAAT ACCCACTGGAATCTGGGGTACCTCTTCCAAGCCCTATGGATTTAATGTATAAAATCTTGGTGAAAAACAA GAAGAAGTCGCACAAGTCGTCAGAGGGAAGTGGTAAGAAGAAGCTTTCAGAGCAAGCTTCCAACACCTAC AGCGACTCCTCCAGTGTGTTCGAGCCTTCGTCTCCAGGAGCTGGGGAAGCAGATACGGAGAGTGATGACG ATGACGACGACGATGACTGTAAAAAGTCTTCCATGGATGAGGGGACAGCTGGCAGCGAAGCCATGGCCAC AGAAGAGATGTCTAACCTGGTGAACTATATTCAGCCTGTCAAGTTTGAGTCCTTTGAAATTTCAAAAAAA AGAAATAAAAGCTTTGAAATGTCTTCCTTCGTGGAAACCAAAGGACTTGAACAACTTACAAAGTCTCCGG TGGAATTTGTAGAATACAACAAGATGCAACTTAGCAGGATATATCCCAAGGGAACACGTGTGGATTCATC CAACTACATGCCTCAACTCTTCTGGAATGCTGGCTGTCAGATGGTGGCGCTCAACTTCCAGACAGTGGAT CTAGCTATGCAGATAAACATGGGCATGTATGAGTACAATGGGAAGAGCGGCTATAGGCTGAAGCCAGAGT TCATGAGGAGGCCGGACAAGCATTTTGATCCATTTACTGAAGGAATCGTAGATGGGATAGTGGCCAACAC TCTATCTGTTAAGATTATTTCAGGTCAGTTCCTCTCTGATAAGAAAGTTGGGACTTATGTGGAAGTGGAT ATGTTTGGCTTGCCTGTGGACACAAGGAGGAAGGCATTTAAAACCAAGACATCCCAAGGAAATGCTGTAA ACCCTGTCTGGGAAGAAGAACCTATTGTATTCAAAAAGGTAGTTCTACCTTCTCTGGCCTGTTTGAGGAT AGCAGCATATGAAGAGGGAGGCAAATTTATTGGCCACCGGATATTGCCTGTGCAGGCTATTCGGCCAGGC TATCACTACATCTGCCTGCGGAATGAGAGGAACCAGCCCCTGACGCTGCCAGCTGTCTTTGTCTACATAG AAGTCAAAGATTATGTGCCAGACACGTATGCAGATGTAATCGAAGCTCTATCAAACCCAATCCGATATGT CAATCTGATGGAACAGAGAGCTAAGCAGCTGGCTGCGTTGACCCTGGAGGATGAAGAGGAAGTAAAGAAG GAGGCTGACCCTGGAGAAACATCTTCCGAGGCTCCAAGCGAAACCAGGACAACGCCAGCAGAGAATGGGG TGAATCACACCGCATCCCTTGCACCCAAGCCGCCTTCCCAGGCTCCACACAGCCAGCCTGCTCCAGGGTC TGTGAAGGCACCTGCCAAAACAGAAGATCTGATCCAGAGCGTGTTAACAGAAGTAGAGGCGCAAACCATC GAAGAGCTCAAGCAACAGAAATCGTTCGTGAAACTTCAAAAGAAACACTACAAAGAAATGAAAGACCTGG TGAAGAGACACCACAAGAAAACCACGGAGCTCATTAAGGAGCACACGACCAAGTACAACGAGATTCAGAA TGACTACCTGAGAAGGAGGGCAGCCTTGGAGAAGTCCGCCAAAAAGGACAGCAAGAAGAAATCTGAACCC AGCAGCCCTGACCACGGCTCATCAGCCATTGAGCAAGACCTCGCTGCCCTGGATGCAGAAATGACTCAGA AGTTGATAGACTTGAAAGACAAGCAACAACAGCAGCTGCTTAATCTTCGGCAAGAGCAGTATTACAGCGA GAAGTACCAAAAGCGAGAACACATTAAACTGCTTATTCAGAAGTTGACAGATGTTGCAGAAGAGTGTCAG AACAATCAGTTAAAGAAGCTGAAGGAAATCTGCGAGAAAGAGAAGAAGGAATTAAAGAAGAAAATGGATA AGAAGAGGCAAGAGAAGATAACAGAAGCCAAGTCCAAAGACAAAAGCCAGATGGAAGAGGAGAAGACAGA GATGATCCGATCGTACATCCAGGAGGTGGTTCAGTACATCAAGAGGTTAGAGGAAGCACAAAGTAAAAGA CAAGAAAAACTCGTGGAGAAACACAATGAGATCCGCCAGCAGATCCTCGATGAGAAGCCCAAGCTGCAGA CGGAGCTGGAGCAAGAATACCAAGACAAGTTCAAAAGGCTGCCCCTGGAGATTCTGGAGTTTGTACAGGA AGCCATGAAAGGGAAGATCAGCGAGGACAGCAATCACGGCTCTGCCCCTCCCTCTCTGGCCTCAGATGCT GCAAAGGTGAACCTCAAGTCTCCCTCCAGTGAGGAGATAGAAAGAGAGAACCCAGGAAGAGAGTTTGATA CTCCTCTG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA Protein Sequence (showhide) >MG211812 representing BC058710 Red=Cloning site Green=Tags(s) MAGAQPGVHALQLKPVCVSDSLKKGTKFVKWDDDSTIVTPIILRTDPQGFFFYWTDQNKETELLDLSLVK DARCGKHAKAPKDPKLRELLDVGNIGHLEQRMITVVYGPDLVNISHLNLVAFQEEVAKEWTNEVFSLATN LLAQNMSRDAFLEKAYTKLKLQVTPEGRIPLKNIYRLFSADRKRVETALEACSLPSSRNDSIPQEDFTPD VYRVFLNNLCPRPEIDNIFSEFGAKSKPYLTVDQMMDFINLKQRDPRLNEILYPPLKQEQVQVLIEKYEP NSSLAKKGQMSVDGFMRYLSGEENGVVSPEKLDLNEDMSQPLSHYFINSSHNTYLTAGQLAGNSSVEMYR QVLLSGCRCVELDCWKGRTAEEEPVITHGFTMTTEISFKEVIEAIAECAFKTSPFPILLSFENHVDSPKQ QAKMAEYCRLIFGDALLMEPLEKYPLESGVPLPSPMDLMYKILVKNKKKSHKSSEGSGKKKLSEQASNTY SDSSSVFEPSSPGAGEADTESDDDDDDDDCKKSSMDEGTAGSEAMATEEMSNLVNYIQPVKFESFEISKK RNKSFEMSSFVETKGLEQLTKSPVEFVEYNKMQLSRIYPKGTRVDSSNYMPQLFWNAGCQMVALNFQTVD LAMQINMGMYEYNGKSGYRLKPEFMRRPDKHFDPFTEGIVDGIVANTLSVKIISGQFLSDKKVGTYVEVD MFGLPVDTRRKAFKTKTSQGNAVNPVWEEEPIVFKKVVLPSLACLRIAAYEEGGKFIGHRILPVQAIRPG YHYICLRNERNQPLTLPAVFVYIEVKDYVPDTYADVIEALSNPIRYVNLMEQRAKQLAALTLEDEEEVKK EADPGETSSEAPSETRTTPAENGVNHTASLAPKPPSQAPHSQPAPGSVKAPAKTEDLIQSVLTEVEAQTI EELKQQKSFVKLQKKHYKEMKDLVKRHHKKTTELIKEHTTKYNEIQNDYLRRRAALEKSAKKDSKKKSEP SSPDHGSSAIEQDLAALDAEMTQKLIDLKDKQQQQLLNLRQEQYYSEKYQKREHIKLLIQKLTDVAEECQ NNQLKKLKEICEKEKKELKKKMDKKRQEKITEAKSKDKSQMEEEKTEMIRSYIQEVVQYIKRLEEAQSKR QEKLVEKHNEIRQQILDEKPKLQTELEQEYQDKFKRLPLEILEFVQEAMKGKISEDSNHGSAPPSLASDA AKVNLKSPSSEEIERENPGREFDTPL TRTRPLE - GFP Tag - V
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	BC058710
ORF Size	3648 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC058710, AAH58710
RefSeq Size	4643 bp
RefSeq ORF	3650 bp
Locus ID	18795
Gene Summary	The production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) is mediated by activated phosphatidylinositol-specific phospholipase C enzymes.[UniProtKB/Swiss-Prot Function]

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