Gse1 (BC043094) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	TurboGFP
Synonyms	mKIAA0182, MGC58013, 2210013I18Rik
Vector	pCMV6-AC-GFP
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MG211805 representing BC043094 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGCCATGAGCCCAAGTCCCCTTCCATAGGGATGCTTTCCACGGCGACCAGGACCACCGCCACCGTCA ACCCCCTCACCCCCTCGCCACTCAATGGCGCCCTGGTGCCCACCGGTAGCCCGGCCACCAGCAGTACGCT GTCGGCCCAGGCCGCTCCATCCTCCAGCTTTGCTGCCGCACTGCGCAAGCTCGCCAAACAGGCAGAGGAG CCCAGAGGGTCCTCACTGAGCAGCGAGTCATCTCCAGTGTCCTCTCCAGCCACCAACCATAGCTCTCCAG CTAGCACGCCCAAACGAGTGCCCATGGGCCCCATCATCGTCCCCCCCGGGGGTCACAGTGTCCCCAGCAC TCCCCCAGTGGTCACTATTGCCCCCACTAAAACCGTCAATGGAGTCTGGAGAAGCGAGAGCCGCCAGGAC TCTGGCTCACGAGGCAGCAGCAGTGGTCGGGAGCGCCTATTGGTGGAGCCACCTCTCGCCCAGGAAAAGG CTGCGGGCCCCGCCATCCCCTCCCACCTACTCAGCACCCCGTACCCCTTTGGCCTTTCCCCCGGCTCAGT TGTACAGGACTCCCGCTTCCAACCACTCAACCTCCAGCGGCCTGTGCACCATGTGGTGCCCCCCAGCACG GTGACTGAGGACTACTTGAGAAGCTTCCGGCCCTACCACACTGCTGAAGACTTACGCATGTCCTCCTTGC CTCCGCTTGGCCTGGACCCAGCCACCGCCGCAGCCTACTATCACCCCAGCTACCTGGCCCCACACCCATT CCCCCACCCGGCCTTCAGGATGGATGACTCCTATTGCCTGTCAGCCTTAAGGTCTCCGTTCTACCCCATC CCCACCCCTGGCTCCCTGCCTCCACTGCATCCGTCGGCGATGCACCTGCATCTCTCCGGCGTACGGTATC CTCCTGAGCTCTCCCACTCGTCCCTGGCGGCGCTACACTCGGAGCGGATGTCCAGCCTCAGTGCAGAGAG GTTGCAAATGGACGAGGAACTGCGGCGGGAGAGAGAGCGAGAGCGGGAACGGGAACGGGAGGCCGACCGG GAGCGGGAGAAGGAGCGGGAGCGGGAGCAGCGGGAGAAGGAGCGGGAGAAGGAGCTGGAGCGCGAGCGGG AGAAGGAACGGGAGCGCGAGCTGGAGCGGCAGCGGGAGCAGCGGGCGAGGGAGAAGGAGCTGCTGGCTGC CAAGGCCTTAGAGCCCACCACCTTCCTGCCTGTGGCCGAGCTGCACGGACTCCGAGGTCACAGCACGGAG GAGCGGCCCAAGCCCTCGGAGCAGCTGACCCCAACCCGAGCAGAGAAGCTGAAGGACGTGGGCTTGCAGG CGCCCAAGCCCGTGCAGCACCCTCTGCACCCAGTGCCTGCCCCACACCACACGGTGCCCAGCCTCATCTC CAGCCACGGCATCTTCTCTCTGCCTGGAAGCAGCGCCACCACCGCCCTGCTGATCCAGCGCACCAACGAG GAGGAGAAGTGGCTGGCACGGCAGCGCCGCCTGCGGCAGGAGAAGGAAGACCGCCAGTCCCAGGTGTCCG AGTTCCGGCAGCAGGTCCTGGAGCAGCACCTAGACCTGGGCCGGCCCCTGGTGCCCACAGAGGCGGAGCA CAGGCCCGAGAGCACCAGGCCAGGAACAAACCGGCATGAGCAGGGCAGCCGTGAACCCCCACAGCACTTT GGTGGCCCTCCACCCCTCATCTCACCCAAGCCCCAGCAGCACACCGTACCCACAGCCCTCTGGAACCCAG TGTCTCTGATGGACAATGCTCTGGAGACGCGGCGGGCCGAGAGCCACTCTCTGCATAGCCACCCGACTGC TTTTGAGCCCAGCCGCCAGGCCGCCGTGCCACTGGTGAAGGTGGAGCGAGTTTATTGCTCAGAGAAGGCA GAGGAGCCCCGGAAGCGTGAGGCCACACCACTGGACAAATACCAGCCACCACCACCACCACCACCACCGC GGGAGGCAGGAAGTCTGGAGCCTCAGACCTTTCCCCACGGACCTGGGCCTTTCCTTACTGAACTTGAAAA GTCAACACAGACCATCTTGGGCCAGCAGCGGCCCTCTCTTTCCCAGGCAACCTCCTTCGGGGAGCTCAGT GGGCCTCTGAAACCAGGCTCTCCGTACTGCCACCCCACAGCGAGGGGCCCCGATCCAGCCTACATCTACG ATGAGTTTCTTCAGCAGCGACGGAAGCTGGTCAGCAAACTGGACCTGGAGGAACGCAGGCGGCGCGAGGC CCAGGAGAAAGGGTACTACTACGACCTCGATGACTCGTACGACGAGAGTGACGAGGAGGAGGTCAGGGCA CACCTCCGCTGTGTGGCTGAGCAGCCGCCCCTCAAACTGGACACATCCTCGGAGAAGCTAGAGTTCTTGC AACTTTTTGGCTTGACCACCCAACAGCAGAAGGAGGAACTGGTGGCGCAGAAGCGCCGGAAGCGGAGGCG GATGCTCAGAGAGAGAAGCCCGTCGCCACCTGCCGTTCAGTGCAAGCGACAGACGCCTTCCCCCAGGCTG GCTCTGTCCACCCGCTACAGCCCCGACGAGATGAACAACAGCCCCAACTTTGAGGAGAAGAGGAAGTTCC TGACCTTCTTCAACTTGACGCACATCAGTGCGGAGAAGAGGAAAGACAAAGAGAGGCTTGTTGAAATGCT CCGAGCCATGAAGCAGAGAGCACTGTCCGCAGCAGACTCAGTGACAAACTCTTCGAGGGACAGTCCTCCC GTCTCCCTGAGTGAACCAGCCACACAGCCAGCTCCTCTAGAGACGGATCAGCCTGTCGGGGTCCCTGCCT CCTTGTCAGATGTCCCAAAGACCACGGAGACCGGGAGACTGGAACAGCTCCGGCCCCAGGAGCTCTTGAG AGTCCAGGAGCCGGCTCCTCCCAGTGGTGAGAAGGCCAGGCTGAGCGAGGCCCCTGGTGGCAAGAAGAGC CTGAGCATGCTCCATTACCTCCGGGGCGCCGCGCCCAAGGACATTCCTGTGCCGTTGTCTCACAGCATCA ACGGGAAGAGCAAGCCCTGGGAGCCTTTCGTGGCCGAAGAGTTTGCACATCAATTCCACGAGTCCGTGCT GCAGTCCACACAGAAGGCTCTGCAGAAGCATAAAGGGAACTCTGCTTTGCTATCTGCAGAGCAGAGCCAC AAAGTCGACACGGCAATCCACTACAACATTCCCGAGCTGCAGTCCTCCAGCAGGGTCCCCTTGCCCCAGC ACAATGGACAACAGGAGCCTCCAATGGGGAGGAAGGGCCCTCCTATGCAGGAGGCGGACCAGGACTCTGA GGAGGACTCTGAGGAGGACAGCGAGGAGGAAGCTGAGGAAGCCCCGAGGCGCCAGTGGCAAGGGATTGAG GCAATCTTTGAAGCTTACCAGGAACACATAGAAGAGCAAAACCTGGAGCGGCAGGTGCTGCAGACGCAGT GCCGGCGGTTGGAGGCGCAGAACTACAGCCTCAGCCTGACTGCGGAGCAGCTGTCTCACAGCATGGCGGA GCTTCGGAGTCAGAAACAGAAGATGGTCTCTGAGCGGGAGCGGCTCCAGGCGGAGCTGGACCACTTGCGG AAGTGCCTTGCCTTGCCCACCATGCACTGGCCTAGGGGCTACTTTAAGGGATATCCGAGG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA Protein Sequence (showhide) >MG211805 representing BC043094 Red=Cloning site Green=Tags(s) MSHEPKSPSIGMLSTATRTTATVNPLTPSPLNGALVPTGSPATSSTLSAQAAPSSSFAAALRKLAKQAEE PRGSSLSSESSPVSSPATNHSSPASTPKRVPMGPIIVPPGGHSVPSTPPVVTIAPTKTVNGVWRSESRQD SGSRGSSSGRERLLVEPPLAQEKAAGPAIPSHLLSTPYPFGLSPGSVVQDSRFQPLNLQRPVHHVVPPST VTEDYLRSFRPYHTAEDLRMSSLPPLGLDPATAAAYYHPSYLAPHPFPHPAFRMDDSYCLSALRSPFYPI PTPGSLPPLHPSAMHLHLSGVRYPPELSHSSLAALHSERMSSLSAERLQMDEELRREREREREREREADR EREKEREREQREKEREKELEREREKERERELERQREQRAREKELLAAKALEPTTFLPVAELHGLRGHSTE ERPKPSEQLTPTRAEKLKDVGLQAPKPVQHPLHPVPAPHHTVPSLISSHGIFSLPGSSATTALLIQRTNE EEKWLARQRRLRQEKEDRQSQVSEFRQQVLEQHLDLGRPLVPTEAEHRPESTRPGTNRHEQGSREPPQHF GGPPPLISPKPQQHTVPTALWNPVSLMDNALETRRAESHSLHSHPTAFEPSRQAAVPLVKVERVYCSEKA EEPRKREATPLDKYQPPPPPPPPREAGSLEPQTFPHGPGPFLTELEKSTQTILGQQRPSLSQATSFGELS GPLKPGSPYCHPTARGPDPAYIYDEFLQQRRKLVSKLDLEERRRREAQEKGYYYDLDDSYDESDEEEVRA HLRCVAEQPPLKLDTSSEKLEFLQLFGLTTQQQKEELVAQKRRKRRRMLRERSPSPPAVQCKRQTPSPRL ALSTRYSPDEMNNSPNFEEKRKFLTFFNLTHISAEKRKDKERLVEMLRAMKQRALSAADSVTNSSRDSPP VSLSEPATQPAPLETDQPVGVPASLSDVPKTTETGRLEQLRPQELLRVQEPAPPSGEKARLSEAPGGKKS LSMLHYLRGAAPKDIPVPLSHSINGKSKPWEPFVAEEFAHQFHESVLQSTQKALQKHKGNSALLSAEQSH KVDTAIHYNIPELQSSSRVPLPQHNGQQEPPMGRKGPPMQEADQDSEEDSEEDSEEEAEEAPRRQWQGIE AIFEAYQEHIEEQNLERQVLQTQCRRLEAQNYSLSLTAEQLSHSMAELRSQKQKMVSERERLQAELDHLR KCLALPTMHWPRGYFKGYPR TRTRPLE - GFP Tag - V
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	BC043094
ORF Size	3630 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC043094, AAH43094
RefSeq Size	6715 bp
RefSeq ORF	3632 bp
Locus ID	382034

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