Xpo6 (BC058090) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	TurboGFP
Synonyms	2610005L19Rik; AL022631; C230091E20Rik; mKIAA0370; R75304; Ranbp20
Vector	pCMV6-AC-GFP
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MG211663 representing BC058090 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCATCAGAAGAAGCCTCCCTCAGGGCGTTGGAAAGCCTGATGACGGAATTTTTCCATGACTGCACAA CCAATGAAAGGAAGCGGGAGATAGAGGAGCTTCTTAATAACTTTGCCCAGCAAGTAGGAGCTTGGAGATT CTGCTTATATTTTCTGTCCAGCACAAGGAATGACTATGTAATGATGTACAGCTTAACGGTTTTTGAGAAT CTAATCAATAAAATGTGGCTTGGGGTCCCATCTCAGGATAAGATGGAAATCCGTAGCTGCTTGCCCAAAC TTCTCTTGGCTCACCATAAAACCTTACCTTACTTTATTCGGAATAAACTCTGCAAAGTTATCGTTGATAT TGGACGCCAAGATTGGCCCATGTTCTACCATGACTTTTTCACTAACATTTTACAGTTGATCCAGTCCCCT GTGACAACCCCCCTTGGACTGATCATGCTGAAGACAACTTCGGAAGAGCTAGCTTGTCCACGTGAGGACC TCAGCGTGGCTCGGAAGGAGGAGTTGCGGAAGCTGCTGCTGGACCAGGTGCAGACAGTGCTTGGGCTCTT GACAGGTATCTTGGAAACTGTCTGGGACAAACACAGTGTTACTGCTGCCACCCCACCACCATCCCCGACG TCAGGAGAAAGTGGTGATTTACTGAGTAACCTGTTGCAGAGTCCTAGTTCAGCCAAACTGCTGCACCAGC CCATCCCCATCCTGGATGTGGAGAGTGAGTATGTCTGTTCCCTGGCCTTGGAGTGCCTGGCCCACCTCTT CAGTTGGATTCCTCTGTCTGCCAGCATCACCCCATCCCTCCTCACCACTATCTTCCACTTTGCTCGATTT GGCTGCGATACCCGGGCCAGAAAGATGGCATCAGTGAATGGCAGCAGCCAGAACTGTGTTTTGGGTCAGG AGCGTGGCCGACTTGGGGTCTTGGCCATGTCCTGCATCAATGAACTCATGTCCAAGAACTGTGTGCCTAT GGAATTTGAGGAATACTTATTGCGAATGTTTCAACAAACCTTCTACCTCCTGCAAAAAATCACCAAGGAT AACAATGCCCACACGGTGAAGAGCAGGCTAGAGGAACTCGATGAGAGTTACATTGAGAAGTTTACTGACT TCCTGCGGCTCTTTGTGAGTGTTCACCTAAGAAGAATCGAGTCCTATTCCCAGTTCCCGGTGGTGGAGTT CTTGACCCTTCTGTTCAAGTACACGTTTCATCAGCCTACTCATGAAGGTTACTTCTCTTGTTTGGATATC TGGACACTGTTTTTGGACTATCTGACAAGTAAAATTAAAAGTCGTCTCGGAGACAAGGAAGCAGTTCTCA ACAGGTACGAAGATGCTCTGGTCCTCTTGCTTACAGAGGTGTTGAATCGAATCCAGTTCAGATACAACCA GGCCCAACTCGAGGAGCTGGATGATGAGACCCTGGACGACGATCAGACAGAGTGGCAGCGGTACTTACGC CAGAGCTTGGAGGTCGTGGCCAAAGTGATGGAGCTCCTTCCCACACACGCCTTCTCAACCCTGTTCCCAG TTCTTCAGGACAATTTAGAAGTTTATTTGGGATTACAGCAATTTGTAGTTACTTCGGGGTCAGGACACAG GCTGAACATCACAGCGGAGAACGACTGCCGGCGTTTGCACTGTTCCCTGCGAGACTTGAGCTCCCTGCTG CAGGCTGTGGGCCGATTAGCTGAATACTTCATTGGGGATGTGTTTGCTGCAAGGTTCAATGATGCTCTCA CAGTTGTGGAGAGGTTGGTCAAAGTTACTCTGTATGGATCTCAGATAAAACTGTACAACATTGAAACAGC TGTGCCATCAGTGTTGAAACCCGACCTCATTGATGTGCACGCTCAGTCACTTGCGGCTCTCCAGGCCTAC TCCCACTGGCTGGCGCAGTACTGCGGTGAAGCCCATCGACAGAACACACAGCAGTTTGTGACACTCATCT CTACCACAATGGACGCCATCACACCTCTCATCAGCACCAAGGTCCAAGACAAGTTGCTGCTGTCTGCGTG CCACCTACTGGTCTCACTAGCCACCACCGTGCGGCCTGTCTTTCTGATCAGTATCCCTGCAGTGCAGAAG GTGTTCAACAGCATCATTGATGCCTCTGCCCAGCGTCTAACAGATAAGGCGCAGGTGTTGGTTTGCCGTG CACTTTCTAATACCTTGCTCCTTCCATGGCCAAATCTTCCTGAGAGTGAGCAGCAGTGGCCTCTGCGCTC GATCAACCATGCCAGCCTCATCTCTGCACTCTCCCGGGACTACCACAGCCTGAAGCCCAGTGCTACTGCC CCCCAGAGAAAGGTGCCACTTGGTGACACCAAAGTGATTATTCACCAGACACTTAGTGTCTTGGAAGATA TCGTGGAGAATATTTCTGGGGAATCCACCAAGTCTCGACAGATCTGCTACCAGTCACTGCAGGAATCTGT TCAGGTCTCCCTGGCCCTCTTTCCAGCTTTTATCCATCAGTCAGATGTGACTGACGAGATGCTGAGCTTC TTCCTCACCTTGTTCCGAGGCCTTCGAGTACAGATGGGCGTGCCGTTCACAGAGCAGATCATACAGACTT TCCTCAACATGTTTACCAGAGAACAGTTGGCTGAGAGCATCCTCCATGAAGGCAGCACTGGCTGCCGGGT GGTGGAGAAGTTCCTGAAGATCCTACAAGTGGTGGTTCAGGAGCCTGGCCAGGTGTTCAAGCCCTTCCTC CCCAGCATTATCGCGCTGTGCATGGAGCAAGTGTACCCCATCATTGCTGAGCGCCCGTCCCCTGATGTCA AGGCTGAGCTGTTTGAGCTCCTCTTTCGAACGCTCCATCACAACTGGCGGTACTTCTTCAAGTCCACTGT CCTCGCTAGCGTGCAGAGGGGCATCGCTGAGGAGCAGATGGAGAATGAGCCCCAGTTCAGTGCCATCATG CAGGCTTTTGGACAGTCCTTTCTTCAGCCTGACATCCACCTGTTCAAACAAAATCTCTTCTACTTGGAGA CTCTGAACACGAAGCAGAAACTATACCACAAAAAGATCTTCCGGACCACCATGCTGTTCCAGTTCGTGAA CGTGCTGCTCCAGGTCCTGGTTCATAAGTCTCATGACCTTCTGCAGGAGGAGATCGGCATTGCCATTTAC AACATGGCTTCTGTCGACTTTGATGGCTTCTTTGCAGCCTTTCTTCCAGAGTTCCTGACTAGCTGTGATG GTGTGGATGCCAATCAGAAAAACGTTCTGGGGCGGAATTTCAAGATGGATCGGGACCTACCATCGTTCAC CCAGAATGTGCACAGGCTGGTAAATGATCTCCGTTACTACCGACTCTGCAATGACAGCCTGCCCCCTGGC ACCGTGAAGCTC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA Protein Sequence (showhide) >MG211663 representing BC058090 Red=Cloning site Green=Tags(s) MASEEASLRALESLMTEFFHDCTTNERKREIEELLNNFAQQVGAWRFCLYFLSSTRNDYVMMYSLTVFEN LINKMWLGVPSQDKMEIRSCLPKLLLAHHKTLPYFIRNKLCKVIVDIGRQDWPMFYHDFFTNILQLIQSP VTTPLGLIMLKTTSEELACPREDLSVARKEELRKLLLDQVQTVLGLLTGILETVWDKHSVTAATPPPSPT SGESGDLLSNLLQSPSSAKLLHQPIPILDVESEYVCSLALECLAHLFSWIPLSASITPSLLTTIFHFARF GCDTRARKMASVNGSSQNCVLGQERGRLGVLAMSCINELMSKNCVPMEFEEYLLRMFQQTFYLLQKITKD NNAHTVKSRLEELDESYIEKFTDFLRLFVSVHLRRIESYSQFPVVEFLTLLFKYTFHQPTHEGYFSCLDI WTLFLDYLTSKIKSRLGDKEAVLNRYEDALVLLLTEVLNRIQFRYNQAQLEELDDETLDDDQTEWQRYLR QSLEVVAKVMELLPTHAFSTLFPVLQDNLEVYLGLQQFVVTSGSGHRLNITAENDCRRLHCSLRDLSSLL QAVGRLAEYFIGDVFAARFNDALTVVERLVKVTLYGSQIKLYNIETAVPSVLKPDLIDVHAQSLAALQAY SHWLAQYCGEAHRQNTQQFVTLISTTMDAITPLISTKVQDKLLLSACHLLVSLATTVRPVFLISIPAVQK VFNSIIDASAQRLTDKAQVLVCRALSNTLLLPWPNLPESEQQWPLRSINHASLISALSRDYHSLKPSATA PQRKVPLGDTKVIIHQTLSVLEDIVENISGESTKSRQICYQSLQESVQVSLALFPAFIHQSDVTDEMLSF FLTLFRGLRVQMGVPFTEQIIQTFLNMFTREQLAESILHEGSTGCRVVEKFLKILQVVVQEPGQVFKPFL PSIIALCMEQVYPIIAERPSPDVKAELFELLFRTLHHNWRYFFKSTVLASVQRGIAEEQMENEPQFSAIM QAFGQSFLQPDIHLFKQNLFYLETLNTKQKLYHKKIFRTTMLFQFVNVLLQVLVHKSHDLLQEEIGIAIY NMASVDFDGFFAAFLPEFLTSCDGVDANQKNVLGRNFKMDRDLPSFTQNVHRLVNDLRYYRLCNDSLPPG TVKL TRTRPLE - GFP Tag - V
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene
ACCN	BC058090
ORF Size	3372 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC058090, AAH58090
RefSeq Size	4493 bp
RefSeq ORF	3374 bp
Locus ID	74204
Gene Summary	Mediates the nuclear export of actin and profilin-actin complexes in somatic cells.[UniProtKB/Swiss-Prot Function]

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