Stard13 (BC027830) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	TurboGFP
Synonyms	DLC2, GT650, MGC38133
Vector	pCMV6-AC-GFP
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MG211650 representing BC027830 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTTCAGCCAGGTGCCCAGGACGCCGGCTGCAGGCTGCTACTATCTAAACCCCTTGACACCTGAGAGCC AGGAGATGTACTTGCGATTTGATCAGACTGCCAGACGCTCTCCCTACAGGATGAGCCGGATCCTAGCACG CCATCACCTAGTGACTAAAATCCAGCAAGAAATTGAGGCGAAGGAAGCATGTGACTGGCTCCGAGCTGCC GGGTTCCCGCAGTACGCTCAGCTGTATGAAGATTCGCAGTTCCCCATCAACATTGCGGCCGTCAAAAAAG ACCATGACTTTCTTGAAAGGGACCTTGTAGAACCTCTTTGCAGACGACTTAATACGTTGAACAAGTGTGC CTCAATGAGACTTGATGTGAACTTCCAAAGGAAAAAGGGTGACGACTCAGATGAGGAAGACCTGTGCATC AGCAACAAATGGACTTTCCAGAGAACCAGCCGCAGATGGTCCCGTGTGGATGACCTGCATACGCTGTTCC CCGTGGCAGACAGAAACGGGTCCCCAGGAGGCCCTAGGATGAGAAACACAGCCAGCAGTGAGAGCGTGCT CACGGATCTGAGTGAGCCAGAGGTCTGCTCTATTCACAGCGAAAGCAGTGGGGGCAGTGACAGCCGCAGC CAATCAGGGCATCATTCTGCTGACAGTACGCATGCGCTGGAGGCCACCCTGGTCAGTAGCAGCCTCCCAC AGTCTACCCGAGAGGGTCTCAACCAGTCTTTTCACCCCAAGAATGAGAAACCCACCAGGACCAGGGCCAA GTCCTTTCTGAAACGCATGGATACCCTGAGAGTGAAGGGTGCACTTGGGAGGCATAAGGGGCCAGGGCGG ACAGGAGGCCTGGTCATCAGTAGGCCTGTGCTGCAGCAGGAACCGGAGTCCTTTAAGACCATGCAGTGCG TCCAGATACCCAACGGAGATCTGCAGACCTCACCTCCAGCTGCCTGCAGGAAAGGCCTCCCATGCTCCAG TAAGTCAAGTGGTGAGAGCAGCCCCCTGGAGAACAGCAGCACAGTGAGCACTCCGTGCATGAAGGAACGC AAGTGCCACCACGAGGCCAACAAGCGCGGTGGCATGTACCTGGAGGACCTGGATGTGCTGGCAGGGACGG CATTACCAGATACGTCAGACCAAAACCACGTGCATGGGTTTCACTCCCAAGAAAACTTGGTGGTCCACAT TCCCAAGGATCACAAACCAGGAACGTTCCCCAAGGCACTTTCTATAGAAAGCCTCTCACCCACAGACAAC AGCAATGGGGTTAACTGGAGGACCGGGAGTATCTCCCTGGGTAGGCAACAGGGCCCTGGCATGAGGGAAC CCAGACTCATGTCTTCCTGCCACAGGGCCAGCCGTGTCAGTATCTATGACAATGTCCCCAGCTCGCACCT GTATGCCAGCACAGGAGATCTGTTGGACCTGGAGAAAGACGGCCTCCTTCCACAGCTGGATGACATCCTA CAGCATGTCAATGGCATACAAGAGGTAGTGGATGACTGGTCAAAAAACATCTTACCCGAACTGCAAAGTC ACAGCACATTGGCAGGGGATCCTGGTCTGTCCCCATTCCCATCTCCCAATCAGGTCACTTTAGATTTTGA AGGCAACTCTGTCTCAGAAGGTCGGACAACACCTAGTGATGTGGAAAGGGACAGGACTTCTCTGAATGAA TCGGAGGCCACTGGGGTCAGAGAAAGAAGGGATTCTGGTGTGGGGGCCTCTCTGACCAGACCAAACAGAC GACTAAGGTGGAGCAGCTTCCAGCTCTCACACCAGCCCCAGCCGTCTCCAGCCACCCCGCACATCAGCAG CCAGACGGCCGCCCAGCTGAACCTGCTCCAGCGCTTCTCCCTACTTCGTCTTACGGCCATCATGGAGAAG TACTCTATGTCCAACAAGCACGGCTGGACCTGGTCCGTTCCAAAGTTCATGAAGAGGATCAAAGCTCCTG ACTACCGAGACAAGGCTGTCTTCGGTGTTCCGCTCATAGTCCACGTTCAGAGAACAGGACAGCCCCTGCC TCAGAGCATCCAACAAGCACTGAGGTATCTACGTAGCAACTGTCTGGATCAGGTGGGTCTTTTCCGCAAG TCAGGAGTGAAGTCTCGAATCCATGCCCTCCGTCAGATGAATGAGAACTTCCCTGACAATGTGAGCTATG AAGACCAGTCTGCATACGACGTGGCAGATATGGTGAAGCAGTTCTTCCGGGACCTCCCTGAGCCCCTGTT CACCAACAAGCTCAGCGAGACCTTCCTCCACATCTATCAGTATGTCCCCAAAGAGCAGCGGCTGCAGGCC GTGCAGGCAGCCATCCTGCTGCTGGCCGACGAGAACCGGGAGGTCCTGCAGACTCTCCTGTGCTTCCTGC ATGACGTAGTAAACTTGGTGGACGAGAATCAGATGACGCCCATGAATCTGGCCGTGTGCCTGGCCCCCTC CCTCTTTCACCTAAACTTACTGAAGAAAGAAAGCTCCCCAAAAGTCATCCAGAAGAAATACGCCACTGGG AAGCCAGATCAGAAGGACCTCAATGAAAATCTAGCTGCGGCTCAGGGGCTGGCCCACATGATCACGGAAT GCAACCGACTCTTTGAGGTTCCACACGAGATGGTGGCCCAGTCTCGGGACTCCTACTTAGAGGCTGAAAT CCATGTGCCCAGCCTGGAAGACTTGGGAGCACAGCTGGCGGAGAGCGGGGCAACTTTTCACACGTACTTG GAGCATCTAGTCCAAGGCCTCCAGAAAGAGGCTAAGGAGAAATTCAAGGGATGGGTCACATGTTCCAGCC CTGACAACACAGACCTTGCTTTCAAAAAGGTGGGCGATGGGCACCCGCTGAAGCTGTGGAAAGCATCTGT GGAGGTGGAGGCACCACCCTCCGTGGTGCTGAACCGTGTGCTGAGAGAACGTCACCTGTGGGATGAGGAC TTTGTGCAGTGGAAGGTGGTGGAAAGATTGGACAAACAAACGGAAATCTACCAGTACGTGCTAAACAGCA TGGTTCCACATCCCTCCAGAGACTTCCTGGTGCTCAGGACCTGGAAGACTGACCTGCCCAAAGGAATGTG CACCCTGGTGTCCCTGTCTGTGGAGTACGAAGAAGCCCAGCTCATGGGTGGCGTGAGGGCGGTGGTGATG GACTCTCAGTACCTGATAGAACCCTGCGGTTCTGGCAAGTCCAGGCTGACCCACATCTGCAGAATAGACC TGAAGGGCCACTCCCCAGAATGGTACAGCAAAGGCTTTGGACACCTCTGTGCGGCAGAAGTTACCAGAAT TAGGAACTCTTTCCAGCCTCTCGTTGCTGAGGGTCCAGAAACAAAAATC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA Protein Sequence (showhide) >MG211650 representing BC027830 Red=Cloning site Green=Tags(s) MFSQVPRTPAAGCYYLNPLTPESQEMYLRFDQTARRSPYRMSRILARHHLVTKIQQEIEAKEACDWLRAA GFPQYAQLYEDSQFPINIAAVKKDHDFLERDLVEPLCRRLNTLNKCASMRLDVNFQRKKGDDSDEEDLCI SNKWTFQRTSRRWSRVDDLHTLFPVADRNGSPGGPRMRNTASSESVLTDLSEPEVCSIHSESSGGSDSRS QSGHHSADSTHALEATLVSSSLPQSTREGLNQSFHPKNEKPTRTRAKSFLKRMDTLRVKGALGRHKGPGR TGGLVISRPVLQQEPESFKTMQCVQIPNGDLQTSPPAACRKGLPCSSKSSGESSPLENSSTVSTPCMKER KCHHEANKRGGMYLEDLDVLAGTALPDTSDQNHVHGFHSQENLVVHIPKDHKPGTFPKALSIESLSPTDN SNGVNWRTGSISLGRQQGPGMREPRLMSSCHRASRVSIYDNVPSSHLYASTGDLLDLEKDGLLPQLDDIL QHVNGIQEVVDDWSKNILPELQSHSTLAGDPGLSPFPSPNQVTLDFEGNSVSEGRTTPSDVERDRTSLNE SEATGVRERRDSGVGASLTRPNRRLRWSSFQLSHQPQPSPATPHISSQTAAQLNLLQRFSLLRLTAIMEK YSMSNKHGWTWSVPKFMKRIKAPDYRDKAVFGVPLIVHVQRTGQPLPQSIQQALRYLRSNCLDQVGLFRK SGVKSRIHALRQMNENFPDNVSYEDQSAYDVADMVKQFFRDLPEPLFTNKLSETFLHIYQYVPKEQRLQA VQAAILLLADENREVLQTLLCFLHDVVNLVDENQMTPMNLAVCLAPSLFHLNLLKKESSPKVIQKKYATG KPDQKDLNENLAAAQGLAHMITECNRLFEVPHEMVAQSRDSYLEAEIHVPSLEDLGAQLAESGATFHTYL EHLVQGLQKEAKEKFKGWVTCSSPDNTDLAFKKVGDGHPLKLWKASVEVEAPPSVVLNRVLRERHLWDED FVQWKVVERLDKQTEIYQYVLNSMVPHPSRDFLVLRTWKTDLPKGMCTLVSLSVEYEEAQLMGGVRAVVM DSQYLIEPCGSGKSRLTHICRIDLKGHSPEWYSKGFGHLCAAEVTRIRNSFQPLVAEGPETKI TRTRPLE - GFP Tag - V
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	BC027830
ORF Size	3339 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC027830, AAH27830
RefSeq Size	5494 bp
RefSeq ORF	3341 bp
Locus ID	243362
Gene Summary	May function as a GTPase-activating protein.[UniProtKB/Swiss-Prot Function]

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