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Ogt (NM_139144) Mouse Tagged ORF Clone
CAT＃: MG211521
- TrueORF®
Ogt (tGFP-tagged) - Mouse O-linked N-acetylglucosamine (GlcNAc) transferase (UDP-N-acetylglucosamine:polypeptide-N-acetylglucosaminyl
ORF Plasmid: DDK
"NM_139144" in other vectors (6)
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CNY 9,220.00
Product images
CNY 1,000.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | 1110038P24Rik; 4831420N21Rik; AI115525; Ogtl |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG211521 representing NM_139144
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGTCTTCCGTGGGCAACGTGGCCGACAGTACAGAACCAACGAAACGTATGCTTTCCTTCCAAGGGT TAGCTGAGTTGGCACATCGAGAATATCAGGCAGGAGATTTTGAGGCAGCTGAGAGACACTGCATGCAGCT CTGGAGACAAGAGCCTGACAATACTGGTGTTCTTTTATTACTTTCATCTATACACTTCCAGTGTCGAAGG CTGGACAGATCTGCTCATTTTAGCACCTTGGCAATTAAACAGAATCCCCTTCTAGCAGAAGCCTATTCGA ATTTGGGAAATGTGTACAAGGAAAGAGGGCAGTTGCAGGAAGCAATCGAGCATTATCGACATGCCTTGCG GCTGAAGCCTGATTTCATTGATGGTTATATTAACCTGGCAGCAGCCTTGGTAGCAGCAGGTGACATGGAA GGAGCAGTACAAGCCTATGTCTCTGCTCTTCAGTACAATCCTGATTTGTACTGTGTTCGCAGTGACCTGG GGAACCTGCTCAAAGCCCTGGGTCGCTTGGAAGAAGCCAAGGCATGTTATTTGAAAGCAATTGAGACGCA ACCAAACTTTGCAGTAGCCTGGAGTAATCTCGGCTGTGTTTTCAATGCACAAGGGGAGATTTGGCTGGCT ATTCATCACTTTGAAAAGGCTGTCACCCTTGACCCAAATTTTCTGGATGCTTATATCAATTTAGGAAATG TCTTGAAAGAGGCACGCATTTTTGACAGAGCTGTCGCAGCTTATCTTCGTGCCTTAAGTTTGAGCCCAAA TCATGCGGTGGTGCACGGCAACCTGGCTTGTGTGTACTACGAGCAAGGCCTAATAGACCTGGCCATTGAT ACCTACAGGAGAGCTATCGAACTGCAACCCCATTTCCCCGATGCTTACTGCAACCTAGCAAATGCTCTCA AAGAGAAGGGCAGTGTTGCTGAAGCAGAAGATTGTTATAACACAGCTCTTCGTCTGTGTCCTACTCATGC AGACTCTTTGAATAACCTTGCCAACATCAAACGGGAACAGGGCAACATTGAAGAGGCAGTTCGCCTGTAT CGCAAAGCATTAGAAGTCTTCCCAGAGTTTGCTGCTGCACATTCCAATTTAGCAAGTGTACTGCAACAGC AGGGCAAGCTGCAGGAAGCACTGATGCACTATAAAGAAGCCATACGAATTAGTCCTACATTTGCTGATGC TTATTCCAATATGGGAAACACTCTAAAGGAGATGCAGGATGTGCAGGGCGCTTTGCAGTGTTATACTCGT GCCATCCAGATTAATCCTGCCTTTGCTGATGCACACAGCAATCTGGCCTCCATTCACAAGGATTCAGGGA ATATCCCAGAAGCAATAGCTTCTTACCGCACAGCTCTGAAACTTAAGCCTGACTTTCCTGATGCTTATTG TAACTTGGCTCATTGCCTACAGATTGTCTGTGATTGGACAGACTATGATGAGCGGATGAAGAAATTGGTT AGTATTGTAGCTGAGCAGCTAGAGAAGAATAGACTGCCTTCTGTCCATCCTCACCATAGCATGCTGTACC CTCTTTCCCATGGCTTCAGGAAGGCTATTGCAGAGAGGCATGGGAATCTCTGCTTGGATAAGATTAATGT CCTTCATAAACCACCATATGAACATCCAAAAGACTTGAAGCTCAGTGATGGCCGATTGCGTGTAGGCTAT GTGAGTTCTGACTTCGGGAATCACCCTACTTCACACCTTATGCAGTCTATTCCAGGCATGCATAATCCTG ATAAGTTTGAGGTATTCTGCTATGCCTTGAGCCCGGATGATGGTACAAACTTTCGAGTGAAGGTGATGGC GGAAGCCAATCATTTCATTGATCTTTCTCAGATTCCTTGTAATGGAAAAGCAGCCGACCGCATCCACCAA GATGGAATTCACATCCTTGTGAATATGAATGGGTATACCAAGGGTGCTCGGAATGAGCTCTTTGCTCTTA GGCCAGCTCCTATTCAGGCCATGTGGCTGGGCTACCCTGGGACTAGTGGTGCACTGTTCATGGATTACAT CATCACTGATCAGGAAACTTCCCCAGCTGAAGTTGCAGAGCAGTATTCTGAGAAACTGGCTTATATGCCC CATACTTTCTTTATTGGTGATCATGCTAATATGTTCCCTCACCTGAAGAAAAAAGCAGTCATCGATTTTA AATCCAATGGGCACATTTATGATAATCGGATAGTTCTGAATGGCATCGATCTCAAAGCATTTCTCGATAG CCTACCCGATGTGAAGATTGTCAAGATGAAATGTCCTGATGGAGGTGACAATCCAGACAGCAGTAACACA GCTCTTAATATGCCCGTTATTCCCATGAATACGATTGCAGAAGCAGTAATTGAAATGATTAACAGAGGGC AGATTCAGATAACAATTAACGGATTCAGTATTAGCAATGGACTGGCGACTACACAGATTAATAATAAGGC TGCAACCGGAGAGGAAGTTCCCCGTACCATTATTGTAACCACCCGTTCCCAGTATGGGCTACCAGAAGAT GCCATTGTGTACTGTAACTTTAATCAGTTATATAAAATTGACCCATCTACCCTGCAGATGTGGGCAAATA TTCTGAAACGTGTGCCTAACAGCGTGCTTTGGCTGTTGCGTTTTCCAGCAGTAGGAGAACCCAATATTCA ACAATATGCACAAAATATGGGCCTTCCCCAGAACCGTATCATTTTCTCACCTGTGGCTCCTAAAGAGGAG CATGTCAGGAGAGGTCAGCTGGCTGATGTCTGCCTGGATACTCCTTTGTGTAATGGACACACCACAGGGA TGGATGTTCTCTGGGCAGGAACACCCATGGTGACTATGCCAGGAGAGACTCTTGCCTCTCGAGTTGCAGC TTCTCAGCTTACTTGTCTAGGATGTCTCGAGCTCATTGCTAAAAGCAGACAGGAATATGAAGACATAGCT GTGAAACTGGGAACCGATCTAGAATACCTGAAGAAAATTCGTGGCAAAGTCTGGAAACAGAGAATATCTA GCCCTCTGTTCAACACCAAACAATACACAATGGAATTAGAGCGACTTTATCTGCAGATGTGGGAGCATTA TGCAGCTGGCAACAAACCTGACCACATGATTAAGCCTGTTGAAGTCACCGAGTCAGCC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG211521 representing NM_139144
Red=Cloning site Green=Tags(s) MASSVGNVADSTEPTKRMLSFQGLAELAHREYQAGDFEAAERHCMQLWRQEPDNTGVLLLLSSIHFQCRR LDRSAHFSTLAIKQNPLLAEAYSNLGNVYKERGQLQEAIEHYRHALRLKPDFIDGYINLAAALVAAGDME GAVQAYVSALQYNPDLYCVRSDLGNLLKALGRLEEAKACYLKAIETQPNFAVAWSNLGCVFNAQGEIWLA IHHFEKAVTLDPNFLDAYINLGNVLKEARIFDRAVAAYLRALSLSPNHAVVHGNLACVYYEQGLIDLAID TYRRAIELQPHFPDAYCNLANALKEKGSVAEAEDCYNTALRLCPTHADSLNNLANIKREQGNIEEAVRLY RKALEVFPEFAAAHSNLASVLQQQGKLQEALMHYKEAIRISPTFADAYSNMGNTLKEMQDVQGALQCYTR AIQINPAFADAHSNLASIHKDSGNIPEAIASYRTALKLKPDFPDAYCNLAHCLQIVCDWTDYDERMKKLV SIVAEQLEKNRLPSVHPHHSMLYPLSHGFRKAIAERHGNLCLDKINVLHKPPYEHPKDLKLSDGRLRVGY VSSDFGNHPTSHLMQSIPGMHNPDKFEVFCYALSPDDGTNFRVKVMAEANHFIDLSQIPCNGKAADRIHQ DGIHILVNMNGYTKGARNELFALRPAPIQAMWLGYPGTSGALFMDYIITDQETSPAEVAEQYSEKLAYMP HTFFIGDHANMFPHLKKKAVIDFKSNGHIYDNRIVLNGIDLKAFLDSLPDVKIVKMKCPDGGDNPDSSNT ALNMPVIPMNTIAEAVIEMINRGQIQITINGFSISNGLATTQINNKAATGEEVPRTIIVTTRSQYGLPED AIVYCNFNQLYKIDPSTLQMWANILKRVPNSVLWLLRFPAVGEPNIQQYAQNMGLPQNRIIFSPVAPKEE HVRRGQLADVCLDTPLCNGHTTGMDVLWAGTPMVTMPGETLASRVAASQLTCLGCLELIAKSRQEYEDIA VKLGTDLEYLKKIRGKVWKQRISSPLFNTKQYTMELERLYLQMWEHYAAGNKPDHMIKPVEVTESA TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_139144 |
| ORF Size | 3138 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_139144.4, NP_631883.2 |
| RefSeq Size | 5384 bp |
| RefSeq ORF | 3141 bp |
| Locus ID | 108155 |
| UniProt ID | Q8CGY8 |
| Gene Summary | Catalyzes the transfer of a single N-acetylglucosamine from UDP-GlcNAc to a serine or threonine residue in cytoplasmic and nuclear proteins resulting in their modification with a beta-linked N-acetylglucosamine (O-GlcNAc) (PubMed:29465778). Glycosylates a large and diverse number of proteins including histone H2B, AKT1, EZH2, PFKL, KMT2E/MLL5, MAPT/TAU and HCFC1. Can regulate their cellular processes via cross-talk between glycosylation and phosphorylation or by affecting proteolytic processing. Probably by glycosylating KMT2E/MLL5, stabilizes KMT2E/MLL5 by preventing its ubiquitination (By similarity).Involved in insulin resistance in muscle and adipocyte cells via glycosylating insulin signaling components and inhibiting the 'Thr-308' phosphorylation of AKT1, enhancing IRS1 phosphorylation and attenuating insulin signaling (By similarity). Involved in glycolysis regulation by mediating glycosylation of 6-phosphofructokinase PFKL, inhibiting its activity. Component of a THAP1/THAP3-HCFC1-OGT complex that is required for the regulation of the transcriptional activity of RRM1. Plays a key role in chromatin structure by mediating O-GlcNAcylation of 'Ser-112' of histone H2B: recruited to CpG-rich transcription start sites of active genes via its interaction with TET proteins (TET1, TET2 or TET3). As part of the NSL complex indirectly involved in acetylation of nucleosomal histone H4 on several lysine residues. O-GlcNAcylation of 'Ser-75' of EZH2 increases its stability, and facilitating the formation of H3K27me3 by the PRC2/EED-EZH2 complex (By similarity). Regulates circadian oscillation of the clock genes and glucose homeostasis in the liver. Stabilizes clock proteins ARNTL/BMAL1 and CLOCK through O-glycosylation, which prevents their ubiquitination and subsequent degradation. Promotes the CLOCK-ARNTL/BMAL1-mediated transcription of genes in the negative loop of the circadian clock such as PER1/2 and CRY1/2 (PubMed:23337503, PubMed:23395176). O-glycosylates HCFC1 and regulates its proteolytic processing and transcriptional activity (By similarity). Regulates mitochondrial motility in neurons by mediating glycosylation of TRAK1 (By similarity). Glycosylates HOXA1 (PubMed:29465778).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC223356 | Ogt (untagged) - Mouse O-linked N-acetylglucosamine (GlcNAc) transferase (UDP-N-acetylglucosamine:polypeptide-N-acetylglucosaminyl transferase) (Ogt), (10ug) |
CNY 13,490.00 |
|
| MR211521 | Ogt (Myc-DDK-tagged) - Mouse O-linked N-acetylglucosamine (GlcNAc) transferase (UDP-N-acetylglucosamine:polypeptide-N-acetylglucosaminyl transferase) (Ogt) |
CNY 10,936.00 |
|
| MR211521L1 | Lenti ORF clone of Ogt (Myc-DDK-tagged) - Mouse O-linked N-acetylglucosamine (GlcNAc) transferase (UDP-N-acetylglucosamine:polypeptide-N-acetylglucosaminyl transferase) (Ogt) |
CNY 10,260.00 |
|
| MR211521L2 | Lenti ORF clone of Ogt (mGFP-tagged) - Mouse O-linked N-acetylglucosamine (GlcNAc) transferase (UDP-N-acetylglucosamine:polypeptide-N-acetylglucosaminyl transferase) (Ogt) |
CNY 13,336.00 |
|
| MR211521L3 | Lenti ORF clone of Ogt (Myc-DDK-tagged) - Mouse O-linked N-acetylglucosamine (GlcNAc) transferase (UDP-N-acetylglucosamine:polypeptide-N-acetylglucosaminyl transferase) (Ogt) |
CNY 10,260.00 |
|
| MR211521L4 | Lenti ORF clone of Ogt (mGFP-tagged) - Mouse O-linked N-acetylglucosamine (GlcNAc) transferase (UDP-N-acetylglucosamine:polypeptide-N-acetylglucosaminyl transferase) (Ogt) |
CNY 10,260.00 |
