Usp38 (BC054404) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	TurboGFP
Synonyms	4631402N15Rik; 4833420O05Rik; AA536967; AU044701; AW544820; mKIAA1891
Vector	pCMV6-AC-GFP
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MG211511 representing BC054404 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGACAAGATTCTGGAAGGCCTGGTGAGCTCCTCGCACCCGCTGCCCCTCAAGCGGATGATTGTGCGGA AGGTGGTGGAGTTCGCGGAGCATTGGCTCGACGAGGCGCAGTGCGAGGCCATGTTTGACCTGACGACCCG CCTCATCCTGGAGGGCCAGGACCCTTTCCAGCGACAAGTGGGCCACCAGGTGCTGGAGGCCTACGCGCGG TACCACCGGCCGGAGTTCGAGTCCTTCTTCAACAAGACTTTCGTGTTGGGCCTCCTTCAGCAGGGCTACC ACTCAGTGGACAGGAAGGACGTCGCCATCCTGGACTACATTCACAACGGCCTGAAGCTGATTATGAGCTG CCCGTCGGTGCTGGATCTCTTCAGCCTGCTCCAGGTGGAGGTCCTGCGAATGGTTTGCGAGAGGCCGGAG CCAGTGCTCTGTGCCCGGCTGAGCGACCTCCTGACCGATTTCGTGCAGTGCGTCCCCAAAGGGAAACTGT CAGTTACCTTCTGTCAACAGCTGGTTCGAACGATAGGTCATTTCCAGTGCGTGTCCACTCAGGAGAAGGA ACTACGGGAGTATGTCTCCCAGGTAACGAAAGTGAGTACCTTACTACAGAATATTTGGAAGGCTGAGCCC TCCACGCTGCTACCTTCTCTGCAAGAAGTTTTTGCAAGCATCTCTTCCACAGATGCATCCTTTGAGCCCT CGGTTGCCTTGGCAAGCCTGGTGCAGCATATTCCTCTGCAGATGATCACAGTCCTCATCAGGAGCCTAAC CACCGACCCAAATGTAAAAGATGCGAGTATGACCCAAGCTCTCTGCAGAATGATTGACTGGCTGTCCTGG CCATTGGCTCAGCATGTAGATACGTGGGTAATTGCTCTCCTGAAAGGACTGGCAGCTGTTCAGAAGTTTA CTATTTTGATAGATGTTACTTTGCTGAAAATAGAACTGGTTTTTAACCGACTTTGGTTTCCTCTCGTGAG ACCTGGTGCTCTTGCAGTCCTCTCTCATATGCTGCTTAGTTTTCAGCATTCTCCTGAGGCATTCCATGTG ATTGTCCCTCATATAGTTAACTTGGTTCACTCCTTCAGAAGTGATGGCCTACCTTCCAGCACAGCCTTCC TAGTACAGTTAACAGAGTTGGTTCACTGTATGATGTACCATTACTCCGGATTCCCAGATCTCTATGAGCC TATTCTGGAGGCAGTAAAGGACTTTCCTAAGCCCAGTGAAGAAAAGATTAAGCTGATTCTCAATCAAAGC GCCTGGACTTCTCAATCCAATGCTTTGGCATCTTGCTTGTCTAGACTTTCTGGAAAATCTGAAACTGGAA AAACTGGTCTTATTAACCTGGGAAACACGTGTTACATGAACAGTGTTTTACAAGCATTGTTTATGGCTAC AGAGTTCAGAAGACAAGTATTATCTTTAAATCTAAATGGGTGTAATTCGTTAATGAAAAAATTACAACAT CTTTTTGCTTTTTTGGCTCATACACAAAGGGAAGCTTACGCACCTCGGATATTTTTTGAGGCTTCCAGAC CTCCGTGGTTTACTCCCAGGTCACAACAGGACTGCTCTGAGTACCTCAGGTTTCTGCTGGACAGACTCCA TGAAGAAGAAAAGATCTTGCGAGTTCAGTCCTCACATAAGCCTTCGGAAGGTCTGGACTGTGCTGAAACT TGTTTACAAGAGGTGACCAGTAAGGTAGCAGTGCCCACAGAGTCTCCTGGTACAGGTGACAGTGAGAAGA CTCTAATAGAGAAAATGTTTGGAGGGAAGCTACGGACTCACATCTGCTGCCTAAACTGTGGGAGTACCTC ACACAAAGTTGAAGCCTTCACAGATCTTTCGCTAGCCTTTTGTCCTAGCCCTTCTGTGGAAGACCTGTCT TTCCAAGATACAGCTTCGTTACCCAGTGCTCAAGATGATGGGCTAATGCAAACCAGTGTCGCTGACCCAG AAGAAGAGCCAGTAGTTTATAATCCAGCAACAGCTGCCTTTGTCTGCGACTCAGTTGTGAATCAAAGAGT ATTAGGCAGTCCTCCTGTTGAGTTTCACTGTGCTGAAAGCTCTTCTGTCCCTGAAGAATCTGCAAAGATT CTCATCAGTAAAGATGTACCTCAGAACCCAGGAGGTGAAAGCACAACTTCAGTGACTGACTTACTAAACT ACTTTCTGGCTCCAGAGGTTCTTACTGGGGAGAACCAATACTACTGTGAAAGCTGTGCCTCTCTACAGAA CGCAGAGAAAACTATGCAAATCACGGAGGAGCCTGAGTATCTTATCCTTACGCTCCTGAGATTCTCCTAC GATCAGAAGTACCATGTGAGAAGAAAAATTTTAGACAATGTGTCACTGCCTCTGGTTTTGGAGTTGCCAG TTAAAAGAACTGCTTCCTTCTCTTCCTTGTCCCAGAGTTGGTCTGTAGATGTTGACTTCACTGATATTAA TGAGAACCTACCTAAAAAATTAAAACCTTCTGGGACTGAAGAAGCTTTCTGCCCCAAATTAGTGCCCTAC CTATTAAGTTCTGTTGTTGTGCACTCTGGTGTGTCCTCTGAGAGTGGCCATTACTACTCTTATGCCAGAA ACATCACAGGTACAGAGTCTTCATACCAGATGTGCCCCCAGTCTGAAAGTCTGGCATTAGCACCCTCCCA GAGTTGTTTACTAGGGGTAGAAAGCCCCAATACAGTCATTGAACAGGACTTGGAAAATAAGGAAATGTCA CAAGAGTGGTTTTTATTTAATGACAGCAGGGTGACATTTACTTCATTTCAGTCAGTTCAGAAAATCACAA GTAGGTTTCCAAAAGACACAGCTTATGTGCTTTTGTACAAAAAACAAAGCAGAGCTAATGGCATAGACAG TGACAATCCAGCCAGTGGTGTCTGGGCCAATGGAGATCCACCCCTACAGAAAGAGCTGATGGATGCCATA ACAAAGGACAACAAGCTGTATTTGCAGGAACAAGAATTGAATGCTCGAGCCCGGGCCCTGCAAGCTGCAT CTGCTTCCTGTTCGTTCCGACCCAATGGATTTGATGACAATGACCCACCAGGAAGCTGTGGGCCAACTGG TGGGGGCGGTGGAGGAGGCTTCAATACAGTTGGCAGACTCGTATTT ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA Protein Sequence (showhide) >MG211511 representing BC054404 Red=Cloning site Green=Tags(s) MDKILEGLVSSSHPLPLKRMIVRKVVEFAEHWLDEAQCEAMFDLTTRLILEGQDPFQRQVGHQVLEAYAR YHRPEFESFFNKTFVLGLLQQGYHSVDRKDVAILDYIHNGLKLIMSCPSVLDLFSLLQVEVLRMVCERPE PVLCARLSDLLTDFVQCVPKGKLSVTFCQQLVRTIGHFQCVSTQEKELREYVSQVTKVSTLLQNIWKAEP STLLPSLQEVFASISSTDASFEPSVALASLVQHIPLQMITVLIRSLTTDPNVKDASMTQALCRMIDWLSW PLAQHVDTWVIALLKGLAAVQKFTILIDVTLLKIELVFNRLWFPLVRPGALAVLSHMLLSFQHSPEAFHV IVPHIVNLVHSFRSDGLPSSTAFLVQLTELVHCMMYHYSGFPDLYEPILEAVKDFPKPSEEKIKLILNQS AWTSQSNALASCLSRLSGKSETGKTGLINLGNTCYMNSVLQALFMATEFRRQVLSLNLNGCNSLMKKLQH LFAFLAHTQREAYAPRIFFEASRPPWFTPRSQQDCSEYLRFLLDRLHEEEKILRVQSSHKPSEGLDCAET CLQEVTSKVAVPTESPGTGDSEKTLIEKMFGGKLRTHICCLNCGSTSHKVEAFTDLSLAFCPSPSVEDLS FQDTASLPSAQDDGLMQTSVADPEEEPVVYNPATAAFVCDSVVNQRVLGSPPVEFHCAESSSVPEESAKI LISKDVPQNPGGESTTSVTDLLNYFLAPEVLTGENQYYCESCASLQNAEKTMQITEEPEYLILTLLRFSY DQKYHVRRKILDNVSLPLVLELPVKRTASFSSLSQSWSVDVDFTDINENLPKKLKPSGTEEAFCPKLVPY LLSSVVVHSGVSSESGHYYSYARNITGTESSYQMCPQSESLALAPSQSCLLGVESPNTVIEQDLENKEMS QEWFLFNDSRVTFTSFQSVQKITSRFPKDTAYVLLYKKQSRANGIDSDNPASGVWANGDPPLQKELMDAI TKDNKLYLQEQELNARARALQAASASCSFRPNGFDDNDPPGSCGPTGGGGGGGFNTVGRLVF TRTRPLE - GFP Tag - V
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	BC054404
ORF Size	3126 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC054404, AAH54404
RefSeq Size	4711 bp
RefSeq ORF	3128 bp
Locus ID	74841
Gene Summary	Deubiquitinating enzyme exhibiting a preference towards 'Lys-63'-linked ubiquitin chains.[UniProtKB/Swiss-Prot Function]

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