Rnf111 (BC069835) Mouse Tagged ORF Clone
CAT＃: MG211379
- TrueORF®
Rnf111 (tGFP-tagged) - Mouse ring finger 111 (cDNA clone MGC:76599 IMAGE:30094289)
ORF Plasmid: DDK
"BC069835" in other vectors (4)
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CNY 6180.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | ARK, Arkadia |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG211379 representing BC069835
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCTCAATGGACTCCTGAATTTAACGAGCTCTACACCCTAAAAGTGGCTATGAAGAGTGGGACTCCTG ATGCGCCCACTACACAGGAGAGTCTGAAGGCGGTCCTTCTGCACCCACAGCCCCTAGGGGCCACCAAGAG CTTCCCTGCAGAAGTGGAGATGATTAATAGCAAAGTGGGGAATGAATTCTCTCATCTGTGTGATGATTCT CAAAAACAAGAGAAGGACATGACTGGCAACCAGCAAGAACAAGAAAAAAGTGGTGTTGTGAGAAAAAAGC GCAAAAGCCAGCAGGCTGGCCCCTCATATGTGCAGAACTGTGTCAAAGAAAATCAGGAAATCCTAGGGCG CAGACAACAGCTAGAGACACCGAGTGATGAGGATAATGACTCTTCCTTAAGTGAGTGTCTGTCTTCTCCC TCATCTAGTCTCCATTTTGGGGGCTCTGATACAGTGACTTCAGATGAGGATAAAGAAGTCTCGGTGAGAC ATACCCAGCCTGTTCTGAGTGCTAAAAGTAGAAGTCACAGTGCAAGGTCTCACAAGTGGCCTCGAACTGA GGCAGATCCTGTGCCAAGTTTGTTAATGAAAAGACCTTGTTTTCATGGCAGTGCACTAAGGAGAGTCACA TGCAGAAAGAGGCTGGTGAAGAGCAGCTCCTCGCAGAGGACACAGAAGCAGAAGGAGAGGATGCTAGTGC AGAGGAAGAAGCGGGAGGCGCTAGCTCAGAGGAAGTATGCTCTTCTCTCCAGCTCCAGTAGCTCCAGTGA GAATGACCTCAGCAGCGATTCCTCGTCCAGCTCCTCCACCGATGGAGAAGAGGACCTGTGTGCATCTGCC AGTGAGAACCCCAGCAACCCCGCTGCTCCCTCAGGAAGTATTGATGAAGATGTTGTGGTGATAGAAGCCT CCTTCACTCCCCAGGTCACTGCCAATGAAGAAATTAATGTTACCTCAACTGACAGTGAAGTGGAGATTGT TACAGTTGGAGAAAGCTATCGGTCTCGTTCAACCCTTGGACACTCCAGATCTCATTGGAGCCAAGGTTCA AGTTCGCATACAGGACGGCCACAGGAGTCTCGAAACCGCAGTAGGATCTCTACTGTTATACAGCCCCTGA GGCAGAATGCAGCAGAAGTTGTGGACCTTACTGTTGATGAAGATGAACCTACTATAGTACCAACCACTTC TGCTAGAATGGACTCACAAACTACTAGTGCTTCCATTAACAATTCAAATCCATCTACCTCTGAGCAAGCC TCTGATACTACTTCAACTGTGGCCAGTAGCCAGCCTTCCACAGTGTCAGAGACTGAAGCTACTCTTACAA GCAATAGTGCAACTGGTTCTTCTGTGGGCGATGACGTGAGGAGAACTGCATCTAGTGCTGTCCCAGAAAG TGGTCCTCCTGCAATGCCAAGGCTACCTTCTTGCTGCCCCCAGCACTCACCATGTGGAGGGACATCACAG AGCCATCATGCATTAGCACACCCACACTCAAGTTGTTTTCAGCAGCATGGCCACCATTTTCAGCATCACC ACCATCACCACCATACTCCCCACCCAGCTGTCCCAGTTTCTCCTTCCTTCAGTGACCCTGCTTGCCCAGT GGAAAGACCTCAAGTACAGGCGCCTTGTGGAGCAAATAGCAGCTCTGGTTCCAGCTACCATGACCAGCAG GCATTGCCAGTCGATCTGAGCAACAGTGCTCTCAGGACTCATGGAAGTGGGGGTTTCCATGGTGCATCTG CTTTTGACCCCTGCTGCCCTGTCACATCTTCCCGGGCTGCAGTCTTTGGCCATCAGGCTGCTGCTGCCCC GACTCAGCCATTATCAATAGATGGCTATGGCTCCAGCATGGTTGCCCAGCCCCAGCCCCAGCCCCCTCCA CAGCCTTCTCTTTCGTCATGTCGGCACTACATGCCACCTCCGTATGCTTCCTTGACAAGACCACTGCACC ATCAAGCCTCAGCTTGCCATCACTCTCATGGAAATGCCCCTCCCCAGACTCAGCCTCCCCCTCAAGTGGA TTACGTGATTCCGCATCCTGTCCATGCTTTCCATTCTCAGATATCTTCTCATGCAGCATCTCACCCTGTG GCACCACCGCCGCCAACACATTTAGGCAGTACAGCGGCACCCATCCCTCAGCATCTTCCTCCTGCTCACC AGCCGATTTCACACCATATTCCAGCCCCAGCACCTTCAGCCCAGAGACTGCACCCTCATGAAGTGATGCA GAGGATGGAAGTTCAGAGGAGGAGGATGATGCAGCATCCAACGCGGGCACATGAACGGCCCCCGCCCCAT CCACATAGGATGCACCCAAACTATGGCCATGGGCATCATATTCATGTGCCTCAAACCATGTCTTCACATC CTCGCCAGGCTCCAGAGAGAACTGCCTGGGAACTGGGGATTGAAGCTGGAGTAACTGCAGCTACCTACAC ACCTGGTGCTCTGCACCCTCACCTGGCCCATTACCACGCCCCGCCTCGACTTCATCACCTCCAGTTAGGA GCACTTCCTTTAATGGTTCCTGACATGGCAGGCTATCCTCACATCCGTTATATTTCTTCAGGATTGGATG GAGCATCATTCAGAGGTCCTTTTAGGGGCAATTTTGAGGAATTGATTCATCTGGAAGAAAGATTAGGAAA TGTCAATCGTGGAGCCTCCCAGGGAACAATTGAAAGATGCACATATCCACATAAATATAAAAAGAGGAAA CTGCACTGCAAACAAGATGGGGAAGAAGGGACTGAGGAAGACACAGAGGAAAAATGTACTATCTGTTTGT CTATTTTAGAGGAAGGTGAAGATGTGAGGCGCCTTCCATGTATGCACCTTTTCCACCAAGTGTGTGTTGA TCAGTGGCTGATCACCAATAAGAAGTGCCCCATATGCAGAGTGGACATCGAGGCCCAGCTGCCAAGTGAA AGT ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG211379 representing BC069835
Red=Cloning site Green=Tags(s) MSQWTPEFNELYTLKVAMKSGTPDAPTTQESLKAVLLHPQPLGATKSFPAEVEMINSKVGNEFSHLCDDS QKQEKDMTGNQQEQEKSGVVRKKRKSQQAGPSYVQNCVKENQEILGRRQQLETPSDEDNDSSLSECLSSP SSSLHFGGSDTVTSDEDKEVSVRHTQPVLSAKSRSHSARSHKWPRTEADPVPSLLMKRPCFHGSALRRVT CRKRLVKSSSSQRTQKQKERMLVQRKKREALAQRKYALLSSSSSSSENDLSSDSSSSSSTDGEEDLCASA SENPSNPAAPSGSIDEDVVVIEASFTPQVTANEEINVTSTDSEVEIVTVGESYRSRSTLGHSRSHWSQGS SSHTGRPQESRNRSRISTVIQPLRQNAAEVVDLTVDEDEPTIVPTTSARMDSQTTSASINNSNPSTSEQA SDTTSTVASSQPSTVSETEATLTSNSATGSSVGDDVRRTASSAVPESGPPAMPRLPSCCPQHSPCGGTSQ SHHALAHPHSSCFQQHGHHFQHHHHHHHTPHPAVPVSPSFSDPACPVERPQVQAPCGANSSSGSSYHDQQ ALPVDLSNSALRTHGSGGFHGASAFDPCCPVTSSRAAVFGHQAAAAPTQPLSIDGYGSSMVAQPQPQPPP QPSLSSCRHYMPPPYASLTRPLHHQASACHHSHGNAPPQTQPPPQVDYVIPHPVHAFHSQISSHAASHPV APPPPTHLGSTAAPIPQHLPPAHQPISHHIPAPAPSAQRLHPHEVMQRMEVQRRRMMQHPTRAHERPPPH PHRMHPNYGHGHHIHVPQTMSSHPRQAPERTAWELGIEAGVTAATYTPGALHPHLAHYHAPPRLHHLQLG ALPLMVPDMAGYPHIRYISSGLDGASFRGPFRGNFEELIHLEERLGNVNRGASQGTIERCTYPHKYKKRK LHCKQDGEEGTEEDTEEKCTICLSILEEGEDVRRLPCMHLFHQVCVDQWLITNKKCPICRVDIEAQLPSE S TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | BC069835 |
| ORF Size | 2943 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | BC069835, AAH69835 |
| RefSeq Size | 4725 bp |
| RefSeq ORF | 2945 bp |
| Locus ID | 93836 |
| Gene Summary | E3 ubiquitin-protein ligase required for mesoderm patterning during embryonic development (PubMed:11298452). Acts as an enhancer of the transcriptional responses of the SMAD2/SMAD3 effectors, which are activated downstream of BMP (PubMed:14657019). Acts by mediating ubiquitination and degradation of SMAD inhibitors such as SMAD7, inducing their proteasomal degradation and thereby enhancing the transcriptional activity of TGF-beta and BMP (PubMed:14657019). In addition to enhance transcription of SMAD2/SMAD3 effectors, also regulates their turnover by mediating their ubiquitination and subsequent degradation, coupling their activation with degradation, thereby ensuring that only effectors 'in use' are degraded (By similarity). Activates SMAD3/SMAD4-dependent transcription by triggering signal-induced degradation of SNON isoform of SKIL (By similarity). Associates with UBE2D2 as an E2 enzyme (By similarity). Specifically binds polysumoylated chains via SUMO interaction motifs (SIMs) and mediates ubiquitination of sumoylated substrates (PubMed:23530056). Catalyzes 'Lys-63'-linked ubiquitination of sumoylated XPC in response to UV irradiation, promoting nucleotide excision repair (By similarity). Mediates ubiquitination and degradation of sumoylated PML (PubMed:23530056). The regulation of the BMP-SMAD signaling is however independent of sumoylation and is not dependent of SUMO interaction motifs (SIMs) (PubMed:23530056).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC220666 | Rnf111 (untagged) - Mouse ring finger 111 (cDNA clone MGC:76599 IMAGE:30094289), (10ug) |
CNY 9120.00 |
|
| MR211379 | Rnf111 (Myc-DDK-tagged) - Mouse ring finger 111 (cDNA clone MGC:76599 IMAGE:30094289) |
CNY 7184.00 |
|
| MR211379L3 | Lenti ORF clone of Rnf111 (Myc-DDK-tagged) - Mouse ring finger 111 (cDNA clone MGC:76599 IMAGE:30094289) |
CNY 7510.00 |
|
| MR211379L4 | Lenti ORF clone of Rnf111 (mGFP-tagged) - Mouse ring finger 111 (cDNA clone MGC:76599 IMAGE:30094289) |
CNY 7510.00 |
Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody
Mouse monoclonal turboGFP antibody, clone OTI2H8
