Ifih1 (BC080200) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	TurboGFP
Synonyms	Helicard, Hlcd, MDA5, MDA-5
Vector	pCMV6-AC-GFP
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MG211363 representing BC080200 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCGATTGTCTGTTCTGCAGAGGACAGCTTCAGGAATCTCATCTTATTCTTCAGGCCCAGGCTGAAAA TGTACATTCAGGTGGAGCCAGTGCTGGACCACCTCATCTTTCTGTCTGCAGAAACCAAAGAGCAGATTCT TAAAAAGATCAACACCTGTGGTAACACCAGCGCGGCAGAACTGCTGCTGAGCACCTTGGAGCAGGGACAG TGGCCTCTGGGATGGACGCAGATGTTCGTGGAGGCCCTAGAGCACAGTGGCAATCCCCTAGCCGCGCGCT ATGTCAAACCCACACTCATTGATCTGCCCTCTCCTTCCTCTGAGACTGCCCATGACGAGTGTCTCCACTT GCTGACCCTCCTCCAGCCCACTTTGGTGGACAAACTTCTGATTAACGATGTCTTGGACACTTGCTTCGAG AAGGGACTCTTGACAGTCGAAGACAGAAATCGGATTTCTGCTGCAGGAAACAGCGGGAATGAGTCAGGTG TAAGAGAGCTGCTGAGAAGGATTGTGCAGAAGGAAAACTGGTTTTCTACCTTCCTGGATGTTCTGCGCCA AACTGGAAATGATGCACTATTCCAAGAACTAACAGGTGGAGGCTGCCCAGAAGACAACACAGAATCAGAC ACAAGTTTGGCAGAAGGAAGTGTCAGCTGCTTCGATGAAAGTCTTGGACATAACAGCAACATGGGCAGGG ATTCAGGCACCATGGGAAGTGATTCAGATGAAAGTGTCATCCAGACAAAAAGAGTATCCCCCGAGCCAGA ACTGCAGCTCAGGCCTTACCAAATGGAAGTGGCCCAACCAGCTCTAGATGGGAAGAATATTATTATCTGC CTCCCCACGGGGAGTGGGAAAACCAGAGTGGCTGTTTACATCACCAAAGATCACTTAGACAAGAAGAAGC AGGCATCTGAATCCGGGAAGGTTATCGTTCTTGTCAATAAGGTAATGTTAGCAGAACAACTTTTCCGAAA AGAGTTCAACCCATATTTGAAGAAATGGTATCGAATTATTGGATTAAGTGGCGATACCCAGCTGAAAATA TCATTTCCAGAAGTTGTCAAATCTTACGATGTTATTATCAGCACTGCTCAAATCCTTGAAAACTCCCTCT TAAATCTGGAGAGTGGAGACGATGACGGTGTGCAGCTGTCAGACTTCTCTCTCATTATCATTGATGAGTG CCATCACACCAACAAGGAGGCAGTCTATAACAACATCATGAGACGATATTTGAAGCAGAAGCTGAGAAAC AATGACCTCAAGAAACAAAACAAACCAGCCATTCCCCTGCCGCAGATACTAGGACTGACAGCTTCACCTG GTGTTGGAGCAGCCAAAAAGCAGTCTGAGGCTGAAAAACATATTTTAAATATATGTGCCAATCTTGATGC CTTTACCATTAAAACAGTGAAAGAGAATCTTGGTCAACTCAAACACCAAATAAAGGAACCATGCAAGAAA TTTGTGATTGCTGATGACACCAGAGAAAATCCATTTAAAGAGAAACTTCTAGAAATTATGGCAAGCATTC AGACTTACTGCCAAAAAAGTCCAATGTCAGATTTTGGAACCCAACATTATGAGCAGTGGGCCATTCAAAT GGAGAAAAAAGCTGCTAAAGACGGAAATCGCAAAGATCGCGTCTGTGCAGAACATTTGAGGAAGTACAAC GAAGCCCTACAAATCAACGACACGATCCGAATGATTGATGCATATAGCCACCTGGAGACATTCTACACTG ATGAGAAAGAAAAGAAGTTCGCAGTCCTCAATGACAGCGACGAGAGTGATGACGAGGCCAGCAGTTGCAA TGACCAACTTAAGGGCGATGTAAAGAAATCTTTGAAACTGGACGAAACGGATGAATTTCTCATGAATTTG TTCTTTGATAACAAGAAAATGTTGAAAAAACTAGCTGAAAACCCAAAATACGAGAATGAAAAACTCATTA AATTAAGAAACACGATACTGGAACAATTCACAAGGTCTGAGGAGTCCTCCCGAGGAATTATTTTTACAAA AACACGACAGAGCACCTACGCACTTTCCCAGTGGATCATGGAAAATGCAAAGTTTGCGGAAGTTGGAGTC AAAGCGCATCACCTGATTGGCGCGGGGCACAGCAGTGAAGTCAAGCCCATGACTCAGACTGAACAAAAAG AAGTCATTAGTAAATTTCGCACTGGCGAAATAAATCTGCTTATCGCTACGACGGTGGCAGAGGAAGGCCT GGATATCAAAGAGTGCAATATTGTTATTCGTTATGGCCTTGTCACGAACGAGATAGCCATGGTCCAGGCC CGGGGTCGAGCCAGAGCTGATGAAAGCACGTATGTCCTGGTCACCAGCAGTGGCTCAGGAGTTACCGAAC GGGAGATTGTTAATGATTTCCGAGAGAAGATGATGTATAAAGCTATTAACCGTGTTCAAAACATGAAACC AGAGGAGTATGCACATAAGATTTTGGAATTGCAGGTGCAAAGTATCCTGGAAAAGAAAATGAAAGTCAAA AGAAGCATTGCAAAGCAATACAACGACAATCCATCGTTAATAACACTTCTCTGCAAAAATTGTAGCATGC TGGTCTGCTCGGGAGAAAACATCCATGTCATTGAGAAGATGCATCATGTCAATATGACACCAGAATTCAA GGGACTCTACATTGTAAGAGAAAACAAAGCACTGCAAAAGAAATTTGCTGATTATCAGACCAATGGAGAG ATTATCTGCAAGTGTGGCCAGGCTTGGGGAACAATGATGGTGCACAAAGGTTTAGATTTGCCTTGTCTTA AAATAAGGAATTTTGTAGTCAATTTCAAAAATAACTCACCGAAGAAACAGTACAAGAAGTGGGTGGAATT GCCTATCAGATTTCCTGATCTTGACTACTCAGAATACTGCTTGTATAGTGATGAAGAT ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA Protein Sequence (showhide) >MG211363 representing BC080200 Red=Cloning site Green=Tags(s) MSIVCSAEDSFRNLILFFRPRLKMYIQVEPVLDHLIFLSAETKEQILKKINTCGNTSAAELLLSTLEQGQ WPLGWTQMFVEALEHSGNPLAARYVKPTLIDLPSPSSETAHDECLHLLTLLQPTLVDKLLINDVLDTCFE KGLLTVEDRNRISAAGNSGNESGVRELLRRIVQKENWFSTFLDVLRQTGNDALFQELTGGGCPEDNTESD TSLAEGSVSCFDESLGHNSNMGRDSGTMGSDSDESVIQTKRVSPEPELQLRPYQMEVAQPALDGKNIIIC LPTGSGKTRVAVYITKDHLDKKKQASESGKVIVLVNKVMLAEQLFRKEFNPYLKKWYRIIGLSGDTQLKI SFPEVVKSYDVIISTAQILENSLLNLESGDDDGVQLSDFSLIIIDECHHTNKEAVYNNIMRRYLKQKLRN NDLKKQNKPAIPLPQILGLTASPGVGAAKKQSEAEKHILNICANLDAFTIKTVKENLGQLKHQIKEPCKK FVIADDTRENPFKEKLLEIMASIQTYCQKSPMSDFGTQHYEQWAIQMEKKAAKDGNRKDRVCAEHLRKYN EALQINDTIRMIDAYSHLETFYTDEKEKKFAVLNDSDESDDEASSCNDQLKGDVKKSLKLDETDEFLMNL FFDNKKMLKKLAENPKYENEKLIKLRNTILEQFTRSEESSRGIIFTKTRQSTYALSQWIMENAKFAEVGV KAHHLIGAGHSSEVKPMTQTEQKEVISKFRTGEINLLIATTVAEEGLDIKECNIVIRYGLVTNEIAMVQA RGRARADESTYVLVTSSGSGVTEREIVNDFREKMMYKAINRVQNMKPEEYAHKILELQVQSILEKKMKVK RSIAKQYNDNPSLITLLCKNCSMLVCSGENIHVIEKMHHVNMTPEFKGLYIVRENKALQKKFADYQTNGE IICKCGQAWGTMMVHKGLDLPCLKIRNFVVNFKNNSPKKQYKKWVELPIRFPDLDYSEYCLYSDED TRTRPLE - GFP Tag - V
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	BC080200
ORF Size	2930 bp
OTI Disclaimer	Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	BC080200, AAH80200
RefSeq Size	3635 bp
RefSeq ORF	2930 bp
Locus ID	71586
Gene Summary	Innate immune receptor which acts as a cytoplasmic sensor of viral nucleic acids and plays a major role in sensing viral infection and in the activation of a cascade of antiviral responses including the induction of type I interferons and proinflammatory cytokines. Its ligands include mRNA lacking 2'-O-methylation at their 5' cap and long-dsRNA (>1 kb in length). Upon ligand binding it associates with mitochondria antiviral signaling protein (MAVS/IPS1) which activates the IKK-related kinases: TBK1 and IKBKE which phosphorylate interferon regulatory factors: IRF3 and IRF7 which in turn activate transcription of antiviral immunological genes, including interferons (IFNs); IFN-alpha and IFN-beta. Responsible for detecting the Picornaviridae family members such as encephalomyocarditis virus (EMCV), mengo encephalomyocarditis virus (ENMG), and theiler's murine encephalomyelitis virus (TMEV). Can also detect other viruses such as dengue virus (DENV), west Nile virus (WNV), and reovirus. Also involved in antiviral signaling in response to viruses containing a dsDNA genome, such as vaccinia virus. Plays an important role in amplifying innate immune signaling through recognition of RNA metabolites that are produced during virus infection by ribonuclease L (RNase L). May play an important role in enhancing natural killer cell function and may be involved in growth inhibition and apoptosis in several tumor cell lines.[UniProtKB/Swiss-Prot Function]

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