Aars (BC033273) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	TurboGFP
Synonyms	MGC37368
Vector	pCMV6-AC-GFP
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MG211342 representing BC033273 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGATGCCACTTTAACAGCACGAGAGATCCGAGAGCGGTTTATAAATTTCTTCAGACGAAATGAGCACA CCTATGTCCACTCTTCCGCCACCATCCCTCTGGATGACCCCACTCTGCTCTTCGCCAACGCTGGCATGAA CCAGTTCAAACCCATCTTCCTAAACACAATCGACCCATCTCACCCTATGGCGAAGCTGAGCAGAGCTGCC AATACCCAGAAATGCATCCGAGCTGGGGGCAAGCACAACGACCTGGATGACGTGGGCAAGGATGTCTACC ACCACACTTTCTTTGAGATGCTGGGCTCCTGGTCTTTTGGGGACTATTTCAAGGAATTGGCATGTAAGAT GGCCCTGGAACTTCTTACCCAGGAGTTTGGTATTCCAGTTGAAAGACTCTATGTCACTTACTTTGGTGGT GATGAGGCGGCCGGCCTAGAGCCAGATCTGGAGTGCAGACAGATTTGGCAAAATTTAGGACTGGATGAAG CCAGAATCCTCCCTGGCAACATGAAGGACAACTTCTGGGAGATGGGTGACACAGGCCCCTGTGGTCCCTG CAGCGAGATCCACTATGACCGCATTGGTGGCAGGGACGCCGCACACCTTGTCAACCAGGATGACCCCAAT GTGCTGGAGATCTGGAACCTCGTGTTCATCCAGTATAACAGGGAGTCGGATGGTGTTCTGAAACCTCTTC CCAAGAAAAGCATCGACACAGGGATGGGCCTAGAGAGGCTGGTGTCTGTGCTGCAGAACAAGATGTCCAA CTACGACACCGACCTTTTCATGCCTTACTTCGAAGCCATTCAGAAGGGCACCGGCGCCCGGCCGTATACT GGGAAGGTTGGTGCTGAGGATGCAGACGGAATCGACATGGCCTACCGGGTTCTGGCTGACCACGCCCGGA CCATCACTGTGGCGCTGGCTGATGGCGGCCGGCCTGACAACACAGGACGGGGGTACGTGCTGAGACGGAT CCTTCGCCGAGCTGTTCGGTATTCCCATGAGAAACTGAATGCCAGCAGGGGTTTCTTCGCTACATTGGTT GATGTTGTCGTTCAATCCCTGGGAGACGCATTTCCTGAGCTGAAGAAGGACCCAGAGATGGTGAAGGACA TCATTAATGAAGAAGAGGTGCAGTTTCTCAAGACTCTCAGCCGAGGGCGGCGCATCCTGGACCGAAAAAT TCAGAGCTTAGGAGACTGCAAAACCATCCCAGGTGACACCGCTTGGCTCCTCTATGATACTTATGGATTC CCAGTGGACCTCACTGGATTGATTGCTGAAGAGAAGGGCCTGGTGGTAGATATGAATGGCTTTGAGGAAG AGAGGAGACTGGCCCAGCTGAAGTCACAGGGCAAGGGAGCAGGGGACGAAGACCTCATCATGCTGGACAT TTACGCTATTGAAGAGCTCCGGGCAAAGGGTTTGGAGGCCACAGATGATTCTCCAAAGTATAACTACCAA TCAGACTCCAGTGGTAGCTACGTGTTTGAGTGTACAGTGGCTACCGTGCTTGCTCTGCGTCGGGAAAAGA TGTTTGTGGACGAGGTGGTCACTGGCCAGGAGTGTGGAGTGGTGCTGGACAAGACCTGTTTCTATGCGGA GCAAGGAGGGCAGATCTACGATGAAGGCTACTTGGTGAAGGTCGACGACAGCAGTGAGGACAAAACCGAG TTTACAGTGAAGAACGCTCAGGTCCGAGGCGGGTACGTGCTGCACATAGGAACGATATATGGTAACCTGA AAGTGGGGGATCAAGTCCGGCTGTTTATTGATGAGCCCCGGCGGAGACCCGTCATGAGCAACCACACAGC TACACACATCCTCAACTTCGCCTTGCGCTCGGTGCTTGGGGAAGCGGACCAGAAAGGTTCACTGGTTGCT CCTGACCGCCTTCGGTTTGACTTCACTGCCAAGGGAGCCATGTCCACCCAGCAAATCAAGAAGGCTGAAG AGATTGTCAATGGCATGATTGAGGCAGCTAAGCCTGTCTACACCCAGGATTGTCCCCTGGCAGCAGCAAA AGCCATCCAGGGCCTTCGAGCTGTGTTTGATGAGACCTATCCTGACCCCGTTCGAGTCGTCTCCATTGGG GTCCCCGTGTCAGAGCTGCTGGATGACCCGTGTGGGCCTGCTGGCTCCCTTACTTCTGTTGAGTTCTGTG GAGGAACACATCTACGGAATTCTAGCCACGCAGGCGCTTTTGTGATAGTGACAGAAGAAGCTATTGCCAA GGGTATCCGGAGGATTGTTGCTGTCACAGGTGCTGAAGCCCAGAAGGCTCTCAGGAAGTCAGAAACCTTG AAGAAATCTCTCTCTGCCATGGAGGCCAAAGTGAAGGCCCAGACTGCACCCAACAAGGACGTGCAGAGGG AAATTGCTGACCTCGGTGAGGCCCTGGCCACTGCAGTCATCCCCCAGTGGCAGAAAGATGAACAACGAGA GACTCTGAAGTCCCTGAAGAAAGTCATGGATGACCTAGACCGGGCCAGCAAAGCCGATGTGCAGAAGCGA GTGTTAGAGAAGACAAAGCAGCTGATTGACAGCAACCCCAACCAGCCGCTGGTCATCCTGGAGATGGAGA GTGGAGCATCGGCCAAGGCCCTGAATGAAGCGTTGAAGCTTTTCAAGACGCATTCTCCTCAGACATCTGC CATGCTGTTCACGGTGGACAATGAGGCTGGCAAGATCACGTGCTTGTGCCAGGTTCCCCAGAATGCAGCC AACCGGGGACTGAAAGCCAGCGAGTGGGTCCAGCAGGTTTCAGGCCTGATGGATGGTAAAGGCGGTGGCA AGGACATGTCCGCCCAGGCCACAGGCAAGAACGTGGGCTGCCTTCAGGAAGCACTGCAGCTGGCTACCTC CTTTGCCCAGCTCCGTCTGGGAGATGTGAAGAAC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA Protein Sequence (showhide) >MG211342 representing BC033273 Red=Cloning site Green=Tags(s) MDATLTAREIRERFINFFRRNEHTYVHSSATIPLDDPTLLFANAGMNQFKPIFLNTIDPSHPMAKLSRAA NTQKCIRAGGKHNDLDDVGKDVYHHTFFEMLGSWSFGDYFKELACKMALELLTQEFGIPVERLYVTYFGG DEAAGLEPDLECRQIWQNLGLDEARILPGNMKDNFWEMGDTGPCGPCSEIHYDRIGGRDAAHLVNQDDPN VLEIWNLVFIQYNRESDGVLKPLPKKSIDTGMGLERLVSVLQNKMSNYDTDLFMPYFEAIQKGTGARPYT GKVGAEDADGIDMAYRVLADHARTITVALADGGRPDNTGRGYVLRRILRRAVRYSHEKLNASRGFFATLV DVVVQSLGDAFPELKKDPEMVKDIINEEEVQFLKTLSRGRRILDRKIQSLGDCKTIPGDTAWLLYDTYGF PVDLTGLIAEEKGLVVDMNGFEEERRLAQLKSQGKGAGDEDLIMLDIYAIEELRAKGLEATDDSPKYNYQ SDSSGSYVFECTVATVLALRREKMFVDEVVTGQECGVVLDKTCFYAEQGGQIYDEGYLVKVDDSSEDKTE FTVKNAQVRGGYVLHIGTIYGNLKVGDQVRLFIDEPRRRPVMSNHTATHILNFALRSVLGEADQKGSLVA PDRLRFDFTAKGAMSTQQIKKAEEIVNGMIEAAKPVYTQDCPLAAAKAIQGLRAVFDETYPDPVRVVSIG VPVSELLDDPCGPAGSLTSVEFCGGTHLRNSSHAGAFVIVTEEAIAKGIRRIVAVTGAEAQKALRKSETL KKSLSAMEAKVKAQTAPNKDVQREIADLGEALATAVIPQWQKDEQRETLKSLKKVMDDLDRASKADVQKR VLEKTKQLIDSNPNQPLVILEMESGASAKALNEALKLFKTHSPQTSAMLFTVDNEAGKITCLCQVPQNAA NRGLKASEWVQQVSGLMDGKGGGKDMSAQATGKNVGCLQEALQLATSFAQLRLGDVKN TRTRPLE - GFP Tag - V
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene
ACCN	BC033273
ORF Size	2904 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC033273, AAH33273
RefSeq Size	4891 bp
RefSeq ORF	2906 bp
Locus ID	234734
Gene Summary	Catalyzes the attachment of alanine to tRNA(Ala) in a two-step reaction: alanine is first activated by ATP to form Ala-AMP and then transferred to the acceptor end of tRNA(Ala) (PubMed:16906134, PubMed:20010690, PubMed:25422440, PubMed:27622773). Also edits incorrectly charged tRNA(Ala) via its editing domain (PubMed:16906134, PubMed:20010690, PubMed:25422440, PubMed:29769718).[UniProtKB/Swiss-Prot Function]

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