Ap2b1 (BC046772) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	TurboGFP
Synonyms	1300012O03Rik; AI788979
Vector	pCMV6-AC-GFP
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MG211286 representing BC046772 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGACTGACTCCAAGTACTTCACAACCAATAAGAAGGGAGAAATTTTTGAATTAAAAGCTGAACTCAACA ATGAAAAGAAAGAAAAGAGGAAGGAGGCTGTGAAGAAAGTGATTGCTGCTATGACCGTGGGGAAGGACGT GAGCTCTCTCTTCCCAGATGTGGTGAACTGCATGCAGACTGACAACCTGGAACTAAAGAAGCTCGTGTAC CTCTATCTGATGAACTATGCCAAGAGTCAGCCAGACATGGCCATCATGGCTGTCAACAGCTTTGTGAAGG ATTGTGAAGATCCCAATCCTTTGATTCGAGCCTTGGCAGTTAGAACCATGGGATGCATCCGGGTGGACAA GATTACAGAGTATCTCTGTGAACCCCTCCGCAAGTGCTTGAAGGATGAAGACCCCTATGTTCGGAAAACG GCAGCAGTATGCGTGGCAAAACTCCATGATATCAATGCCCAGATGGTGGAAGATCAGGGATTTCTGGATT CTCTGCGGGATCTCATAGCAGATTCAAACCCAATGGTGGTGGCTAATGCTGTAGCAGCATTGTCTGAGAT CAGTGAGTCTCACCCAAACAGCAACTTACTTGATCTGAACCCTCAGAATATCAATAAGCTGCTCACAGCC CTGAATGAATGCACTGAATGGGGCCAGATTTTCATCCTGGACTGCCTGTCTAATTACAACCCTAAAGATG ACCGGGAAGCTCAGAGCATCTGTGAGCGAGTAACGCCTCGGCTCTCCCACGCCAACTCTGCAGTGGTGCT TTCAGCCGTAAAAGTCCTAATGAAGTTTCTAGAATTGTTGCCCAAGGACTCTGACTACTACAATATGCTG CTAAAGAAGTTAGCACCTCCACTTGTCACGTTGCTGTCTGGGGAGCCAGAAGTACAGTACGTCGCCCTGA GGAACATCAACCTAATTGTCCAGAAAAGGCCTGAAATCTTGAAGCAGGAAATCAAGGTCTTCTTTGTGAA GTACAATGATCCTATCTATGTTAAACTAGAGAAGTTAGACATCATGATTCGTCTTGCATCCCAAGCCAAC ATTGCTCAGGTTCTGGCGGAGCTGAAGGAATATGCCACTGAAGTTGATGTGGACTTTGTTCGCAAAGCTG TGAGGGCCATTGGACGGTGTGCCATCAAAGTGGAGCAATCAGCAGAACGCTGTGTAAGCACACTGCTTGA TCTAATCCAGACCAAAGTAAATTATGTGGTCCAAGAGGCAATTGTTGTCATCAGGGACATCTTCCGAAAA TACCCCAACAAGTATGAAAGTATTATTGCCACCCTCTGTGAAAACTTGGACTCCCTGGATGAACCCGATG CCCGAGCAGCTATGATTTGGATTGTAGGAGAATATGCTGAAAGAATCGATAACGCAGATGAATTACTAGA GAGTTTCCTGGAAGGTTTTCATGACGAAAGCACCCAGGTGCAGCTCACACTGCTCACCGCCATAGTGAAG CTGTTTCTCAAGAAGCCATCAGAAACACAGGAGCTGGTCCAACAGGTCTTGAGCTTAGCCACACAGGACT CCGATAACCCTGACCTTCGAGATCGGGGTTATATATATTGGCGCCTTCTTTCAACTGACCCTGTGACAGC CAAAGAAGTGGTGTTGTCTGAGAAGCCATTGATCTCTGAGGAGACAGACCTCATTGAGCCTACCCTCCTG GATGAGCTCATCTGCCACATTGGTTCTTTGGCCTCTGTGTACCACAAACCTCCAAATGCTTTTGTGGAAG GGAGTCATGGGATTCATCGCAAACACTTGCCAATTCATCATGGGAGCACTGATGCAGGTGATAGCCCCGT TGGCACCACCACTACAACCAACCTGGAGCAGCCTCAGGTCATTCCCTCTCAAGGTGACCTTCTGGGGGAT CTTTTGAATCTTGACCTGGGTCCCCCAGTGAATGTCCCGCAAGTGTCCTCCATGCAGATGGGAGCGGTGG ATCTTTTAGGAGGAGGACTGGATAGCCTGCTTGGCAGTGACCTTGGCGGGGGCATTGGAGGAAGTCCGGC AGTAGGACAGTCCTTCATCCCGTCATCAGTGCCTGCGACCTTCGCTCCTTCACCTACTCCTGCTGTGGTC AGCAGTGGTCTGAATGACCTGTTTGAGCTTTCCACTGGGATAGGCATGGCACCTGGCGGATATGTGGCTC CTAAGGCGGTCTGGCTACCTGCAGTAAAGGCTAAAGGCTTGGAGATTTCGGGGACGTTTACTCACCGCCA AGGGCATATCTATATGGAAATGAACTTCACCAACAAAGCTCTGCAGCATATGACAGACTTTGCCATCCAG TTTAACAAGAATAGCTTCGGTGTCATCCCAAGCACTCCCTTGGCCATACATACTCCACTGATGCCAAACC AGAGCATTGACGTCTCTCTGCCGCTCAACACCTTGGGCCCAGTCATGAAGATGGAGCCCCTGAATAACCT GCAGGTGGCTGTTAAAAACAATATTGATGTCTTCTACTTCAGCTGCCTCATCCCACTCAATGTGCTTTTT GTAGAAGATGGCAAAATGGAGCGCCAGGTCTTCCTTGCAACGTGGAAGGATATTCCCAATGAAAATGAAC TCCAGTTTCAGATTAAGGAATGTCATTTAAATGCTGACACAGTTTCCAGCAAGTTGCAAAACAACAATGT TTACACTATTGCCAAGAGGAACGTGGAAGGGCAGGACATGCTGTACCAGTCCCTGAAGCTCACTAATGGC ATTTGGATTTTGGCAGAGCTGCGGATCCAGCCAGGAAACCCCAATTATACGCTGTCGCTGAAGTGTAGAG CCCCTGAAGTCTCTCAGTACATCTATCAGGTCTACGACAGCATTTTGAAAAAC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA Protein Sequence (showhide) >MG211286 representing BC046772 Red=Cloning site Green=Tags(s) MTDSKYFTTNKKGEIFELKAELNNEKKEKRKEAVKKVIAAMTVGKDVSSLFPDVVNCMQTDNLELKKLVY LYLMNYAKSQPDMAIMAVNSFVKDCEDPNPLIRALAVRTMGCIRVDKITEYLCEPLRKCLKDEDPYVRKT AAVCVAKLHDINAQMVEDQGFLDSLRDLIADSNPMVVANAVAALSEISESHPNSNLLDLNPQNINKLLTA LNECTEWGQIFILDCLSNYNPKDDREAQSICERVTPRLSHANSAVVLSAVKVLMKFLELLPKDSDYYNML LKKLAPPLVTLLSGEPEVQYVALRNINLIVQKRPEILKQEIKVFFVKYNDPIYVKLEKLDIMIRLASQAN IAQVLAELKEYATEVDVDFVRKAVRAIGRCAIKVEQSAERCVSTLLDLIQTKVNYVVQEAIVVIRDIFRK YPNKYESIIATLCENLDSLDEPDARAAMIWIVGEYAERIDNADELLESFLEGFHDESTQVQLTLLTAIVK LFLKKPSETQELVQQVLSLATQDSDNPDLRDRGYIYWRLLSTDPVTAKEVVLSEKPLISEETDLIEPTLL DELICHIGSLASVYHKPPNAFVEGSHGIHRKHLPIHHGSTDAGDSPVGTTTTTNLEQPQVIPSQGDLLGD LLNLDLGPPVNVPQVSSMQMGAVDLLGGGLDSLLGSDLGGGIGGSPAVGQSFIPSSVPATFAPSPTPAVV SSGLNDLFELSTGIGMAPGGYVAPKAVWLPAVKAKGLEISGTFTHRQGHIYMEMNFTNKALQHMTDFAIQ FNKNSFGVIPSTPLAIHTPLMPNQSIDVSLPLNTLGPVMKMEPLNNLQVAVKNNIDVFYFSCLIPLNVLF VEDGKMERQVFLATWKDIPNENELQFQIKECHLNADTVSSKLQNNNVYTIAKRNVEGQDMLYQSLKLTNG IWILAELRIQPGNPNYTLSLKCRAPEVSQYIYQVYDSILKN TRTRPLE - GFP Tag - V
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	BC046772
ORF Size	2853 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC046772, AAH46772
RefSeq Size	3038 bp
RefSeq ORF	2855 bp
Locus ID	71770
Gene Summary	Component of the adaptor protein complex 2 (AP-2). Adaptor protein complexes function in protein transport via transport vesicles in different membrane traffic pathways. Adaptor protein complexes are vesicle coat components and appear to be involved in cargo selection and vesicle formation. AP-2 is involved in clathrin-dependent endocytosis in which cargo proteins are incorporated into vesicles surrounded by clathrin (clathrin-coated vesicles, CCVs) which are destined for fusion with the early endosome. The clathrin lattice serves as a mechanical scaffold but is itself unable to bind directly to membrane components. Clathrin-associated adaptor protein (AP) complexes which can bind directly to both the clathrin lattice and to the lipid and protein components of membranes are considered to be the major clathrin adaptors contributing the CCV formation. AP-2 also serves as a cargo receptor to selectively sort the membrane proteins involved in receptor-mediated endocytosis. AP-2 seems to play a role in the recycling of synaptic vesicle membranes from the presynaptic surface. AP-2 recognizes Y-X-X-[FILMV] (Y-X-X-Phi) and [ED]-X-X-X-L-[LI] endocytosis signal motifs within the cytosolic tails of transmembrane cargo molecules. AP-2 may also play a role in maintaining normal post-endocytic trafficking through the ARF6-regulated, non-clathrin pathway. The AP-2 beta subunit acts via its C-terminal appendage domain as a scaffolding platform for endocytic accessory proteins; at least some clathrin-associated sorting proteins (CLASPs) are recognized by their [DE]-X(1,2)-F-X-X-[FL]-X-X-X-R motif. The AP-2 beta subunit binds to clathrin heavy chain, promoting clathrin lattice assembly; clathrin displaces at least some CLASPs from AP2B1 which probably then can be positioned for further coat assembly (By similarity).[UniProtKB/Swiss-Prot Function]

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