Scrib (BC060689) Mouse Tagged ORF Clone
CAT＃: MG211217
- TrueORF®
Scrib (tGFP-tagged) - Mouse scribbled homolog (Drosophila) (cDNA clone MGC:66791 IMAGE:6417366)
ORF Plasmid: DDK
"BC060689" in other vectors (4)
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CNY 5890.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | Scrb1, mKIAA0147, CRIB, SCRIB1, vartul |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG211217 representing BC060689
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCTGAAGTGCATCCCGCTCTGGCGTTGTAACCGGCACGTGGAGTCGGTGGATAAGCGGCACTGCTCGC TGCAGGTTGTGCCAGAGGAGATTTACCGCTACAGTCGTAGCCTCGAGGAGCTGCTCCTCGACGCCAACCA GCTGCGCGAGCTACCTAAGCCCTTCTTCCGACTGTTGAACTTGAGAAAGTTGGGCCTCAGTGACAACGAG ATCCAGCGGCTGCCTCCTGAAGTGGCCAACTTCATGCAGCTGGTGGAGCTAGATGTGTCCCGGAACGATA TTCCTGAGATACCTGAGAGCATAAAGTTCTGTAAGGCTCTGGAGATTGCAGACTTCAGTGGGAACCCCCT GTCTAGACTTCCGGATGGCTTCACACAGCTACGCAGCCTGGCTCACCTGGCCCTGAATGACGTGTCCCTG CAGGCACTGCCTGGAGATGTGGGCAACCTGGCCAATCTGGTGACCCTGGAACTCCGGGAGAACCTGCTTA AATCTCTCCCTGCGTCCCTGTCTTTCCTGGTGAAGCTGGAACAGCTGGATCTGGGAGGCAACGACCTGGA AGTGCTGCCTGACACCCTGGGGGCTCTGCCTAACCTTCGGGAGCTATGGTTAGACCGAAACCAACTGTCA GCGCTGCCCCCGGAGCTAGGCAATCTGCGGCGGCTGGTGTGCCTGGATGTGTCAGAGAACAGGCTGGAGG AGCTGCCTGTGGAGTTGGGTGGGCTGGCACTGCTCACGGATCTGCTGCTTTCCCAGAACTTGCTTCAGCG GCTGCCAGAGGGCATCGGTCAGCTGAAGCAGCTGTCTATCCTGAAGGTGGACCAGAACCGGCTGTGTGAG GTCACTGAGGCCATAGGGGACTGTGAGAACCTCTCGGAGCTGATCCTCACGGAGAACCTGTTAACGGCCT TACCCCACTCGCTGGGCAAGCTGACCAAGCTGACTAACCTCAATGTGGACCGGAACCATCTTGAGGTGCT GCCTCCTGAAATCGGAGGCTGTGTGGCCCTTAGTGTCCTCTCTTTGAGAGACAATCGCCTGGCTGTCCTC CCTCCTGAGCTTGCCCATACCGCTGAGCTGCACGTGCTTGATGTGGCTGGGAACCGGCTGCGGAGTCTGC CGTTTGCGCTCACCCACCTCAACCTCAAGGCACTGTGGCTGGCCGAGAACCAGGCACAGCCCATGCTCCG CTTCCAGACTGAGGATGATGCCCAGACGGGTGAAAAGGTGCTCACCTGCTACCTGCTGCCCCAGCAGCCC CTGCCAAGTCTCGAAGACGCTGGACAGCAGAGCAGTCCCTCAGAGAGCTGTAGTGATGCCCCTCTGAGCC GTGTCAGTGTCATCCAGTTCGAGGACACCCTGGAAGGTGAAGAGGATGCTGAGGAAGCTGCGGCCGAGAA GCGGGGCCTACAGCGTAGGGCCACACCTCATCCCAGTGAGCTCAAGGTGATGAAGAGGGGCATTGAAGAG CGCCGGAATGAGGCCTTCGTCTGCAAGCCTGACCCCAGCCCACCCTCGCCTTCAGAGGAGGAGAAGAGGC TGAGTGCAGAGTCTGCCTTGAGTGGAGGCTCTGTCCCGTCAGCAAGCACAGCCTCGGAAGGTGAGCCTGA GATCCTGCCGGCTGAGGTACAAGGGCTAGGCCAGCATGAGGCCATGCCTGCACAGGAGGAGTATACAGAA GACGACTATAACGAGCCCACAGTGCACTTTGCAGAGGACACACTGATCCCTAGGGAAGACGGTGAAAGTG AAGAGGGACAGCCTGAGGCTGCCTGGCCGCTGCCTAGTGGCCGGCAGAGGCTCATCCGCAAAGACACGCC CCACTACAAGAAGCACTTCAAGATCTCCAAGCTCCCACAGCCTGAAGCTGTCGTGGCCCTGCTGCAAGGG GTGCAGACCGACAGGGAGGGCCCCACTGCAGGTTGGCACAATGGCCCACACACACCTTGGGCTCCTCGAG CCCATGAGGAGGAAGAGGAGGAGGAGGAGGAGAACAGGGATGAGGAGGAAGGCGAGGCCACCACGGAAGA AGATGACAAAGAAGAGGCTGTGGCTTCTGCTCCCTCTGTCAAGGGGGTGTCGTTTGACCAGGCCAATAAC CTGCTGATAGAGCCTGCTCGCATTGAGGAGGAAGAGTTAACACTCACGATCGTGCGGCAGACGGGGGGCC TGGGCATCAGTATCGCAGGGGGAAAAGGCTCTACCCCCTACAAAGGAGATGACGAGGGCATTTTCATCTC TCGAGTGTCTGAAGAGGGCCCTGCAGCCCGTGCTGGAGTCCGAGTTGGTGACAAACTTCTTGAGGTGAAT GGTGTAGCCTTGCAGGACGCAGAGCACCACGAGGCCGTGGAAGCACTTCGGGGGGCAGGTGCTGCTGTGC AGATGCGAGTGTGGAGGGAAAGAATGGTGGAGCCGGAGAACGCAGTCACTATTACCCCCTTACGCCCTGA AGATGACTATAGTCCCCGAGAGTGGCGGGGAGGTGGCCTGCGCCTTCCCCTGCTCCAGCCTGAGACTCCT GTATCCCTCCGTCAGCGTCATGCTGCCTGTCTCGTGCGCAGTGAAAAGGGGCTGGGCTTCAGCATTGCTG GTGGAAAGGGCTCCACACCTTACCGGGCTGGTGATGGGGGCATCTTTATATCCCGCATTGCAGAGGGAGG GGCTGCTCACCGGGCAGGCACTCTACAGGTTGGCGACCATCTGCGACCACCGGAGGGAAGACGACTGAGG CTCCCTGTTCCCCTGGCAGTCAGCAACCACCCTCCCCTGATGAACTGCCAGCCAATG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG211217 representing BC060689
Red=Cloning site Green=Tags(s) MLKCIPLWRCNRHVESVDKRHCSLQVVPEEIYRYSRSLEELLLDANQLRELPKPFFRLLNLRKLGLSDNE IQRLPPEVANFMQLVELDVSRNDIPEIPESIKFCKALEIADFSGNPLSRLPDGFTQLRSLAHLALNDVSL QALPGDVGNLANLVTLELRENLLKSLPASLSFLVKLEQLDLGGNDLEVLPDTLGALPNLRELWLDRNQLS ALPPELGNLRRLVCLDVSENRLEELPVELGGLALLTDLLLSQNLLQRLPEGIGQLKQLSILKVDQNRLCE VTEAIGDCENLSELILTENLLTALPHSLGKLTKLTNLNVDRNHLEVLPPEIGGCVALSVLSLRDNRLAVL PPELAHTAELHVLDVAGNRLRSLPFALTHLNLKALWLAENQAQPMLRFQTEDDAQTGEKVLTCYLLPQQP LPSLEDAGQQSSPSESCSDAPLSRVSVIQFEDTLEGEEDAEEAAAEKRGLQRRATPHPSELKVMKRGIEE RRNEAFVCKPDPSPPSPSEEEKRLSAESALSGGSVPSASTASEGEPEILPAEVQGLGQHEAMPAQEEYTE DDYNEPTVHFAEDTLIPREDGESEEGQPEAAWPLPSGRQRLIRKDTPHYKKHFKISKLPQPEAVVALLQG VQTDREGPTAGWHNGPHTPWAPRAHEEEEEEEEENRDEEEGEATTEEDDKEEAVASAPSVKGVSFDQANN LLIEPARIEEEELTLTIVRQTGGLGISIAGGKGSTPYKGDDEGIFISRVSEEGPAARAGVRVGDKLLEVN GVALQDAEHHEAVEALRGAGAAVQMRVWRERMVEPENAVTITPLRPEDDYSPREWRGGGLRLPLLQPETP VSLRQRHAACLVRSEKGLGFSIAGGKGSTPYRAGDGGIFISRIAEGGAAHRAGTLQVGDHLRPPEGRRLR LPVPLAVSNHPPLMNCQPM TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | BC060689 |
| ORF Size | 2787 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | BC060689, AAH60689 |
| RefSeq Size | 4321 bp |
| RefSeq ORF | 2789 bp |
| Locus ID | 105782 |
| Gene Summary | Scaffold protein involved in different aspects of polarized cells differentiation regulating epithelial and neuronal morphogenesis. Most probably functions in the establishment of apico-basal cell polarity. May function in cell proliferation regulating progression from G1 to S phase and as a positive regulator of apoptosis for instance during acinar morphogenesis of the mammary epithelium. May also function in cell migration and adhesion and hence regulate cell invasion through MAPK signaling. May play a role in exocytosis and in the targeting synaptic vesicles to synapses. Functions as an activator of Rac GTPase activity.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC220668 | Scrib (untagged) - Mouse scribbled homolog (Drosophila) (cDNA clone MGC:66791 IMAGE:6417366), (10ug) |
CNY 9120.00 |
|
| MR211217 | Scrib (Myc-DDK-tagged) - Mouse scribbled homolog (Drosophila) (cDNA clone MGC:66791 IMAGE:6417366) |
CNY 6808.00 |
|
| MR211217L3 | Lenti ORF clone of Scrib (Myc-DDK-tagged) - Mouse scribbled homolog (Drosophila) (cDNA clone MGC:66791 IMAGE:6417366) |
CNY 7220.00 |
|
| MR211217L4 | Lenti ORF clone of Scrib (mGFP-tagged) - Mouse scribbled homolog (Drosophila) (cDNA clone MGC:66791 IMAGE:6417366) |
CNY 7220.00 |
Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody
Mouse monoclonal turboGFP antibody, clone OTI2H8
