Tnpo2 (BC003275) Mouse Tagged ORF Clone
CAT＃: MG211064
- TrueORF®
Tnpo2 (tGFP-tagged) - Mouse transportin 2 (importin 3, karyopherin beta 2b) (cDNA clone MGC:6380 IMAGE:3499838)
ORF Plasmid: DDK
"BC003275" in other vectors (4)
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CNY 5510.00
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Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | TRN2, MGC6380, Kpnb2b, IPO3, Knpb2b |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG211064 representing BC003275
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGACTGGCAGCCAGACGAGCAGGGCCTGCAGCAGGTCTTGCAGCTTCTCAAAGACTCGCAGTCGCCCA ACACAGCTACTCAGCGCATTGTGCAGGATAAACTTAAACAACTCAACCAGTTTCCTGACTTTAACAACTA CCTGATCTTTGTCCTGACCAGACTCAAATCAGAAGATGAGCCAACCCGCTCTCTGAGTGGCCTCATCCTC AAGAACAATGTGAAGGCGCATTACCAGAGCTTCCCGCCTCCTGTGGCTGACTTCATCAAACAGGAGTGTC TCAACAACATTGGCGATGCCTCCTCTCTCATCAGAGCCACCATCGGCATTCTCATCACCACCATCGCTTC CAAGGGTGAGCTGCAGATGTGGCCAGAGCTGCTGCCTCAGCTGTGCAACCTGCTCAACTCAGAGGATTAT AATACCTGTGAGGGAGCCTTTGGAGCCCTACAGAAGATCTGTGAAGACTCCTCGGAGCTTTTGGATAGTG ATGCTCTCAACAGGCCCCTCAACATCATGATCCCTAAATTCCTGCAGTTTTTCAAGCACTGCAGCCCTAA GATCCGGTCCCACGCCATCGCCTGTGTGAACCAGTTCATCATGGACCGGGCCCAAGCGCTGATGGACAAC ATTGACACTTTCATTGAGCACCTGTTTGCCTTGGCTGTGGATGATGACCCTGAGGTGAGAAAGAACGTGT GCCGGGCCCTTGTGATGCTTTTGGAAGTGCGAATTGACAGGCTCATCCCTCACATGCACAGCATCATCCA GTACATGCTGCAGAGGACCCAGGACCATGATGAGAACGTGGCCCTGGAGGCCTGTGAGTTCTGGCTGACG CTAGCCGAGCAGCCCATCTGCAAGGAAGTCCTGGCCTCTCATCTGGTCCAGTTGATCCCTATCCTCGTGA ATGGGATGAAGTACTCTGAAATTGACATCATCCTTCTTAAGGGGGATGTGGAGGAGGACGAGGCGGTACC TGACAGTGAGCAGGACATCAAGCCCCGGTTCCACAAGTCACGCACAGTGACATTGACTCACGAGGCTGAG CGGCCTGATAGCTCTGAGGATGCTGAGGACGACGATGATGACGACGCTTTGTCGGACTGGAATCTGAGGA AGTGCTCTGCAGCTGCGCTGGATGTCCTTGCCAATGTCTTCCGGGAGGAACTGTTGCCCCACCTACTTCC CCTGCTCAAGGGCCTCCTATTCCACCCTGAGTGGGTGGTCAAAGAGTCAGGGATCCTGGTGCTGGGCGCC ATCGCTGAGGGCTGCATGCAGGGAATGGTGCCCTATCTACCTGAGCTGATCCCACACCTCATCCAGTGCC TGTCAGATAAGAAGGCTCTAGTACGCTCCATTGCCTGCTGGACGCTGAGCCGCTATGCCCACTGGGTAGT CAGCCAGCCACCTGACATGCACCTCAAGCCTTTGATGACGGAGCTGCTCAAGCGTATCTTGGATGGCAAT AAGCGGGTTCAGGAGGCAGCCTGCAGTGCATTTGCTACCCTGGAGGAGGAGGCATGCACGGAGCTGGTGC CCTACCTCAGCTATATCCTTGACACTCTCGTTTTTGCCTTTGGCAAATACCAGCATAAGAACCTGCTCAT CCTCTATGATGCCATTGGCACCCTGGCCGACTCTGTGGGCCACCACCTCAATCAGCCGGAGTACATTCAG AAGCTGATGCCTCCACTGATCCAGAAGTGGAATGAGCTCAAGGACGAAGACAAGGACCTCTTCCCGCTGC TGGAGTGCCTATCATCTGTGGCCACTGCCCTGCAGAGTGGCTTCCTGCCCTACTGCGAGCCTGTCTACCA GCGCTGTGTTACCCTGGTACAGAAGACACTGGCCCAGGCCATGATGTATACACAACACCCCGAACAATAT GAGGCCCCAGACAAAGACTTCATGATTGTTGCATTGGACCTGCTTAGTGGCTTGGCTGAGGGCCTAGGTG GCCACGTGGAGCAGTTGGTAGCTCGGAGTAACATCATGACATTGCTCTTCCAGTGTATGCAGGATTCCAT GCCTGAGGTCCGGCAGAGCTCCTTTGCCCTTCTGGGAGACCTTACCAAAGCCTGCTTTATCCACGTCAAG CCCTGTATCGCTGAGTTCATGCCTATTCTGGGTACCAACCTGAACCCTGAGTTCATCTCTGTCTGCAACA ATGCTACCTGGGCCATTGGGGAGATCTGCATGCAGATGGGGGCAGAGATGCAGCCTTATGTGCAGATGGT CCTCAACAACCTGGTGGAGATCATTAACAGGCCCAACACACCCAAGACACTGCTGGAAAACACAGCCATC ACCATCGGCCGCCTGGGCTACGTGTGCCCGCAGGAGGTGGCACCCATGCTGCAGCAGTTCATCCGGCCTT GGTGCACGTCCCTTAGGAACATCCGGGACAACGAGGAGAAGGATTCTGCCTTCCGAGGCATCTGCATGAT GATTGGTGTCAATCCTGGGGGTGTTGTGCAGGACTTTATTTTCTTCTGCGATGCTGTAGCCTCCTGGGTG AGCCCGAAGGATGACCTTCGGGACATGTTTTATAAGATCCTCCATGGCTTCAAAGACCAAGTTGGGGAGG AGAACTGGCAACAGTTCTCAGAGCAGTTCCCACCTCTGCTCAAGGAGAGGCTGGCAGCCTTCTATGGGGT C ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG211064 representing BC003275
Red=Cloning site Green=Tags(s) MDWQPDEQGLQQVLQLLKDSQSPNTATQRIVQDKLKQLNQFPDFNNYLIFVLTRLKSEDEPTRSLSGLIL KNNVKAHYQSFPPPVADFIKQECLNNIGDASSLIRATIGILITTIASKGELQMWPELLPQLCNLLNSEDY NTCEGAFGALQKICEDSSELLDSDALNRPLNIMIPKFLQFFKHCSPKIRSHAIACVNQFIMDRAQALMDN IDTFIEHLFALAVDDDPEVRKNVCRALVMLLEVRIDRLIPHMHSIIQYMLQRTQDHDENVALEACEFWLT LAEQPICKEVLASHLVQLIPILVNGMKYSEIDIILLKGDVEEDEAVPDSEQDIKPRFHKSRTVTLTHEAE RPDSSEDAEDDDDDDALSDWNLRKCSAAALDVLANVFREELLPHLLPLLKGLLFHPEWVVKESGILVLGA IAEGCMQGMVPYLPELIPHLIQCLSDKKALVRSIACWTLSRYAHWVVSQPPDMHLKPLMTELLKRILDGN KRVQEAACSAFATLEEEACTELVPYLSYILDTLVFAFGKYQHKNLLILYDAIGTLADSVGHHLNQPEYIQ KLMPPLIQKWNELKDEDKDLFPLLECLSSVATALQSGFLPYCEPVYQRCVTLVQKTLAQAMMYTQHPEQY EAPDKDFMIVALDLLSGLAEGLGGHVEQLVARSNIMTLLFQCMQDSMPEVRQSSFALLGDLTKACFIHVK PCIAEFMPILGTNLNPEFISVCNNATWAIGEICMQMGAEMQPYVQMVLNNLVEIINRPNTPKTLLENTAI TIGRLGYVCPQEVAPMLQQFIRPWCTSLRNIRDNEEKDSAFRGICMMIGVNPGGVVQDFIFFCDAVASWV SPKDDLRDMFYKILHGFKDQVGEENWQQFSEQFPPLLKERLAAFYGV TRTRPLE - GFP Tag - V |
| Restriction Sites | SgfI-MluI Cloning Scheme for this gene |
| ACCN | BC003275 |
| ORF Size | 2661 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | BC003275.1 |
| RefSeq Size | 2897 bp |
| RefSeq ORF | 2663 bp |
| Locus ID | 212999 |
| Gene Summary | Probably functions in nuclear protein import as nuclear transport receptor. Serves as receptor for nuclear localization signals (NLS) in cargo substrates. Is thought to mediate docking of the importin/substrate complex to the nuclear pore complex (NPC) through binding to nucleoporin and the complex is subsequently translocated through the pore by an energy requiring, Ran-dependent mechanism. At the nucleoplasmic side of the NPC, Ran binds to the importin, the importin/substrate complex dissociates and importin is re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran. The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC200150 | Tnpo2 (untagged) - Mouse transportin 2 (importin 3, karyopherin beta 2b) (cDNA clone MGC:6380 IMAGE:3499838), (10ug) |
CNY 6504.00 |
|
| MR211064 | Tnpo2 (Myc-DDK-tagged) - Mouse transportin 2 (importin 3, karyopherin beta 2b) (cDNA clone MGC:6380 IMAGE:3499838) |
CNY 6496.00 |
|
| MR211064L3 | Lenti ORF clone of Tnpo2 (Myc-DDK-tagged) - Mouse transportin 2 (importin 3, karyopherin beta 2b) (cDNA clone MGC:6380 IMAGE:3499838) |
CNY 6940.00 |
|
| MR211064L4 | Lenti ORF clone of Tnpo2 (mGFP-tagged) - Mouse transportin 2 (importin 3, karyopherin beta 2b) (cDNA clone MGC:6380 IMAGE:3499838) |
CNY 6940.00 |
