Mctp2 (BC086658) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	TurboGFP
Synonyms	Gm489
Vector	pCMV6-AC-GFP
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MG211045 representing BC086658 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGATCTGGATAAACCATCTGTCTGGGGATCATTAAAACAGCGAACCAGACCTTTGTTAATCAACCTAA GCAAGAAGAAGGCTAAAAAGAGCCCAAGCAAACCTTTGGATTTAAGGGTGCAGCATCACCTGGACAGGCG CCTCAGCCTCTCAGTGCCTGACCTCCTGGAAGCAGAGGCTTTGGCCCCTGAAGGCCGGCCTTACTCGGGG CCCCAGTCATCCTACATCTCAGTACCCAACAGCCTGTCCACTGCAGGCATTGTCCCGAAGAGCAGCAGCA GCTCACTGAAGCAGTCTGAGGAAGAACTGGACTGGAGCCAGGAGGAAGCAAGCCATGTCCACGGGGTGGA CACCGACTCTGAAGAGATCTATGCCTCTCCCGCAGAAGAGTGGCAGGCATTCAGCCAGAGTGCCCTGGAT CTGCACAAACCTTCCCTTGGAAGAGATGCTCCCGAAGAGCACGACAAGACTCACGGCAATGACGATCTGA ATGCTTCTATGACATCGCAGCATTTTGAAGAAGAATCTACTCTTGGAGAAGCCAGTGATTGTGTGAGTCA CCTCCCCAGCCCCTTCGCGTACCTCCTCACCATACACCTGAAGGAAGGCCGCAACCTCGTGGTCCGGGAT CGCTGTGGCACGAGCGATCCGTATGTGAAATTTAAGCTGAATGGGAAGACACTGTACAAAAGTAAAGTGA TATATAAGAACTTGAACCCAATTTGGGATGAGATAGTTGTGTTGCCAATACAAAGCCTTGATCAAAAGCT CCGTGTGAAGGTGTATGATCGAGACTTAACCAAGTCTGATTTCATGGGTTCTGCATTTGTCGTTCTCAGA GATCTTGAACTTAACAGGACTACAGAGCACATTTTAAAATTGGAAGATCCAAACAGTTTAGAAGACGACA TGGGAGTGATTGTGTTAAATTTGAACCTAGTTGTAAAACAGGGTGATTTCAAGAGGCATCGTTGGTCAAA TCGGAAGCGGTTAAGTGCCAGCAAGTCCTCCTTGATCCGCAACCTACGCCTCTCAGAGTCCCTTCGGAAG AACCAACTCTGGAATGGGATTATAAGTATAACTCTCTTGGAAGGAAAGAATGTCTCAGGAGGGAACATGA CAGAGATGTTTGTCCAGTTAAAACTGGGAGAGCAGAGGTACAAAAGCAAGACACTGTGTAAGAGTGCAAA TCCGCAGTGGCAGGAACAGTTTGACTTTCACTACTTCTCTGACAGGATGGGCATTTTGGACATTGAAGTG TGGGGAAAGGACAGCAAAAAGCATGAGGAGCGACTGGGCACGTGTAAAGTAGATATCTCAGCGCTCCCAC TGAAGCAAGACAATTGCTTGGAGCTGCCTCTGGAGAGCTGCCAGGGGGCTCTCCTCATGTTGATCACCCT GACCCCATGCACTGGAGTCTCCATCTCTGATCTCTGTGTCTGCCCTTTCGAGGATCCCAGTGAAAGACAG CAGATTTCCCAGCGATATGCCTTCCAGAACTCTCTGAAGGATGTGAAGGATGTGGGGATTCTACAGGTGA AGGTTCTGAAGGCCTCGGACCTCCTGGCCGCTGATTTCTCAGGGAAGAGTGATCCTTTCTGCCTATTGGA GTTGGGCAATGACCGACTTCAGACACATACCATTTACAAAAACCTCAATCCTGAGTGGAACAAAGTTTTT ACATTCCCCATTAAAGATATCCATGATGTGCTGGAAGTGACAGTGTTTGATGAGGATGGAGACAAGGCCC CGGACTTTCTTGGAAAAGTCGCCATCCCCTTGCTGTCTATTAGAGATGGACAACCAAACTGTTATGTGTT GAAGAATAAAGACTTAGAGCAAGCTTTTAAAGGGCTTATTTACTTAGAGTTGGACCTCATATATAATCCG GTCAAAGCAAGTATCAGGACCTTCACTCCAAGAGAAAAACGCTTTGTGGAAGACAGCCGCAAGCTGTCCA AAAAGATATTATCAAGAGATGTAGACCGTGTAAAAAGACTCACTTTGGCAATATGGAACACAGTACAGTT CTTCAAAAGCTGCTTCCAATGGGAGTCTACATTAAGGAGTACAATAGCATTTGTGGTGTTTTTGGTCACC GTCTGGAATTTTGAACTCTACATGATCCCTCTGGCATTGTTGCTACTCTTTCTGTACAACTTCCTCAGAC CCATGAAAGGGAAAGCCAGCAGCACCCAGGACAGCCAGGAAAGCACAGACGTAGAGGAGGAAGGGAAAGA GGAAGAAAAGGAGTCTGAGAAAAAGGGGATAATCGAGAGAATCTATATGGTCCAGGATATTGTTTCCACT GTCCAGAACATCTTGGAGGAAGTTGCTTCTTTTGGAGAAAGGATTAAGAACGTGTTTAACTGGACAGTCC CCTTCCTTTCGCTGCTCGCCTGTTTGATTCTGGCCATAACCACTGTCATTTTGTATTTCATCCCTCTACG GTATATCATTCTACTCTGGGGCATAAATAAATTTACTAAGAAGCTTCGAAATCCTTATTCCATCGACAAT AATGAGCTACTAGACTTTCTCTCCAGGGTGCCCTCTGACATTCAGAAGGTGCAGTATGCAGAGCTGAAGC TCTGTGGCAGCCACAGCCCACTTCGGAAGAAGAGGAGTACTGTC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA Protein Sequence (showhide) >MG211045 representing BC086658 Red=Cloning site Green=Tags(s) MDLDKPSVWGSLKQRTRPLLINLSKKKAKKSPSKPLDLRVQHHLDRRLSLSVPDLLEAEALAPEGRPYSG PQSSYISVPNSLSTAGIVPKSSSSSLKQSEEELDWSQEEASHVHGVDTDSEEIYASPAEEWQAFSQSALD LHKPSLGRDAPEEHDKTHGNDDLNASMTSQHFEEESTLGEASDCVSHLPSPFAYLLTIHLKEGRNLVVRD RCGTSDPYVKFKLNGKTLYKSKVIYKNLNPIWDEIVVLPIQSLDQKLRVKVYDRDLTKSDFMGSAFVVLR DLELNRTTEHILKLEDPNSLEDDMGVIVLNLNLVVKQGDFKRHRWSNRKRLSASKSSLIRNLRLSESLRK NQLWNGIISITLLEGKNVSGGNMTEMFVQLKLGEQRYKSKTLCKSANPQWQEQFDFHYFSDRMGILDIEV WGKDSKKHEERLGTCKVDISALPLKQDNCLELPLESCQGALLMLITLTPCTGVSISDLCVCPFEDPSERQ QISQRYAFQNSLKDVKDVGILQVKVLKASDLLAADFSGKSDPFCLLELGNDRLQTHTIYKNLNPEWNKVF TFPIKDIHDVLEVTVFDEDGDKAPDFLGKVAIPLLSIRDGQPNCYVLKNKDLEQAFKGLIYLELDLIYNP VKASIRTFTPREKRFVEDSRKLSKKILSRDVDRVKRLTLAIWNTVQFFKSCFQWESTLRSTIAFVVFLVT VWNFELYMIPLALLLLFLYNFLRPMKGKASSTQDSQESTDVEEEGKEEEKESEKKGIIERIYMVQDIVST VQNILEEVASFGERIKNVFNWTVPFLSLLACLILAITTVILYFIPLRYIILLWGINKFTKKLRNPYSIDN NELLDFLSRVPSDIQKVQYAELKLCGSHSPLRKKRSTV TRTRPLE - GFP Tag - V
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	BC086658
ORF Size	2634 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC086658, AAH86658
RefSeq Size	5810 bp
RefSeq ORF	2636 bp
Locus ID	244049
Gene Summary	Might play a role in the development of cardiac outflow tract.[UniProtKB/Swiss-Prot Function]

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