Epas1 (BC057870) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	TurboGFP
Synonyms	HLF, MOP2, HIF-2alpha
Vector	pCMV6-AC-GFP
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MG211029 representing BC057870 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGACAGCTGACAAGGAGAAAAAAAGGAGCAGCTCAGAGCTGAGGAAGGAGAAATCCCGTGATGCCGCGA GGTGCCGGCGCAGCAAGGAGACGGAGGTCTTCTATGAGTTGGCTCATGAGTTGCCCCTGCCTCACAGTGT GAGCTCCCACCTGGACAAAGCCTCCATCATGCGCCTGGCCATCAGCTTCCTTCGGACACATAAGCTCCTG TCCTCAGTCTGCTCTGAAAATGAATCTGAAGCTGAGGCCGACCAGCAAATGGATAACTTGTACCTGAAAG CCTTGGAGGGTTTCATTGCTGTGGTGACCCAAGACGGTGACATGATCTTTCTGTCGGAAAACATCAGCAA GTTCATGGGACTTACCCAGGTAGAACTAACAGGACACAGCATCTTTGACTTCACTCATCCTTGCGACCAT GAGGAGATCCGTGAGAACCTGACTCTCAAAAACGGCTCTGGTTTTGGGAAGAAGAGCAAAGACGTGTCCA CCGAGCGTGACTTCTTCATGAGGATGAAGTGCACAGTCACCAACAGAGGCCGGACTGTCAACCTCAAGTC GGCCACCTGGAAGGTCCTGCACTGCACCGGGCAAGTGAGAGTCTACAACAACTGCCCCCCTCACAGTAGC CTCTGTGGCTCCAAGGAGCCCCTGCTGTCCTGCCTTATCATCATGTGTGAGCCAATCCAGCACCCATCCC ACATGGACATCCCCCTGGACAGCAAGACTTTCCTGAGCCGCCACAGCATGGACATGAAGTTCACCTACTG TGACGACAGAATCTTGGAACTGATTGGTTACCACCCCGAGGAGCTACTTGGACGCTCTGCCTATGAGTTC TACCATGCCCTGGATTCGGAGAACATGACCAAAAGTCACCAGAACTTGTGCACCAAGGGGCAGGTGGTAT CTGGCCAGTACCGGATGCTAGCCAAACACGGAGGATATGTGTGGCTGGAGACCCAGGGGACGGTCATCTA CAACCCCCGCAACCTGCAGCCTCAGTGTATCATGTGTGTCAACTATGTGCTGAGTGAGATCGAGAAGAAC GACGTGGTGTTCTCCATGGACCAGACCGAATCCCTGTTCAAGCCACACCTGATGGCCATGAACAGCATCT TTGACAGCAGTGACGATGTGGCTGTAACTGAGAAGAGCAACTACCTGTTCACCAAACTGAAGGAGGAGCC CGAGGAACTGGCCCAGTTGGCCCCCACCCCAGGAGATGCCATTATTTCTCTCGATTTCGGAAGCCAGAAC TTCGATGAACCCTCAGCCTATGGCAAGGCCATCCTTCCCCCGGGCCAGCCATGGGCCGCGGGGCTGAGGA GCCACAGTGCCCAGAGCGAGTCCGGGAGCCTGCCAGCCTTCACTGTGCCCCAGGCAGGCACCCCAGGGAA CACTACACCCAGTGCTTCAAGCAGCAGTAGCTGCTCCACGCCCAGCAGCCCTGAGGACTACTATTCATCC TTGGAGAATCCCTTGAAGATCGAAGTGATTGAGAAGCTTTTCGCCATGGACACGGAGCCGAGGGACCCGG GCAGTACCCAGACGGACTTCAGTGAACTGGATTTGGAGACCTTGGCACCCTACATCCCTATGGACGGCGA GGACTTCCAGCTGAGCCCCATCTGCCCAGAGGAGCCGCTCATGCCAGAGAGCCCCCAGCCCACCCCCCAG CACTGCTTCAGTACCATGACCAGCATCTTCCAGCCGCTCACCCCGGGGGCCACCCACGGCCCCTTCTTCC TCGATAAGTACCCGCAGCAGTTGGAAAGCAGGAAGACAGAGTCTGAGCACTGGCCCATGTCTTCCATCTT CTTTGATGCTGGGAGCAAAGGGTCCCTGTCTCCATGCTGTGGCCAGGCCAGCACCCCTCTCTCTTCTATG GGGGGCAGATCCAACACGCAGTGGCCCCCGGATCCACCATTACATTTCGGCCCTACTAAGTGGCCTGTGG GTGATCAGAGTGCTGAATCCCTGGGAGCCCTGCCGGTGGGGTCATCGCAGTTGGAACCTCCGAGCGCCCC GCCTCATGTCTCCATGTTCAAGATGAGGTCTGCAAAGGACTTCGGGGCCCGAGGTCCATACATGATGAGC CCAGCCATGATCGCCCTGTCCAACAAGCTGAAGCTAAAGCGGCAGCTGGAGTATGAGGAGCAAGCCTTCC AAGACACAAGCGGGGGGGACCCTCCAGGCACCAGCAGTTCACACTTGATGTGGAAACGTATGAAGAGCCT CATGGGCGGGACCTGTCCTTTGATGCCTGACAAGACCATCAGTGCGAACATGGCCCCCGATGAATTCACC CAAAAATCTATGAGAGGCCTGGGCCAGCCACTGAGACACCTGCCACCTCCCCAGCCACCATCTACCAGGA ACTCAGGGGAGAACGCCAAGACTGGGTTCCCGCCACAGTGCTATGCCTCCCAGTTCCAGGACTACGGTCC TCCAGGAGCTCAAAAGGTGTCAGGCGTGGCCAGTCGACTGCTGGGGCCATCGTTCGAGCCTTACCTGTTG CCGGAACTGACCAGATATGACTGTGAGGTGAACGTGCCCGTGCCTGGAAGCTCCACACTCCTGCAGGGGA GAGACCTTCTCAGAGCTCTGGACCAGGCCACC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA Protein Sequence (showhide) >MG211029 representing BC057870 Red=Cloning site Green=Tags(s) MTADKEKKRSSSELRKEKSRDAARCRRSKETEVFYELAHELPLPHSVSSHLDKASIMRLAISFLRTHKLL SSVCSENESEAEADQQMDNLYLKALEGFIAVVTQDGDMIFLSENISKFMGLTQVELTGHSIFDFTHPCDH EEIRENLTLKNGSGFGKKSKDVSTERDFFMRMKCTVTNRGRTVNLKSATWKVLHCTGQVRVYNNCPPHSS LCGSKEPLLSCLIIMCEPIQHPSHMDIPLDSKTFLSRHSMDMKFTYCDDRILELIGYHPEELLGRSAYEF YHALDSENMTKSHQNLCTKGQVVSGQYRMLAKHGGYVWLETQGTVIYNPRNLQPQCIMCVNYVLSEIEKN DVVFSMDQTESLFKPHLMAMNSIFDSSDDVAVTEKSNYLFTKLKEEPEELAQLAPTPGDAIISLDFGSQN FDEPSAYGKAILPPGQPWAAGLRSHSAQSESGSLPAFTVPQAGTPGNTTPSASSSSSCSTPSSPEDYYSS LENPLKIEVIEKLFAMDTEPRDPGSTQTDFSELDLETLAPYIPMDGEDFQLSPICPEEPLMPESPQPTPQ HCFSTMTSIFQPLTPGATHGPFFLDKYPQQLESRKTESEHWPMSSIFFDAGSKGSLSPCCGQASTPLSSM GGRSNTQWPPDPPLHFGPTKWPVGDQSAESLGALPVGSSQLEPPSAPPHVSMFKMRSAKDFGARGPYMMS PAMIALSNKLKLKRQLEYEEQAFQDTSGGDPPGTSSSHLMWKRMKSLMGGTCPLMPDKTISANMAPDEFT QKSMRGLGQPLRHLPPPQPPSTRNSGENAKTGFPPQCYASQFQDYGPPGAQKVSGVASRLLGPSFEPYLL PELTRYDCEVNVPVPGSSTLLQGRDLLRALDQAT TRTRPLE - GFP Tag - V
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	BC057870
ORF Size	2622 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	BC057870, AAH57870
RefSeq Size	2998 bp
RefSeq ORF	2624 bp
Locus ID	13819
Gene Summary	Transcription factor involved in the induction of oxygen regulated genes. Heterodimerizes with ARNT; heterodimer binds to core DNA sequence 5'-TACGTG-3' within the hypoxia response element (HRE) of target gene promoters (PubMed:26245371). Regulates the vascular endothelial growth factor (VEGF) expression and seems to be implicated in the development of blood vessels and the tubular system of lung. May also play a role in the formation of the endothelium that gives rise to the blood brain barrier. Potent activator of the Tie-2 tyrosine kinase expression. Activation requires recruitment of transcriptional coactivators such as CREBBP and probably EP300. Interaction with redox regulatory protein APEX seems to activate CTAD (By similarity).[UniProtKB/Swiss-Prot Function]

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