Fbxo41 (BC070445) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	TurboGFP
Synonyms	9630017H13; D6Ertd538e
Vector	pCMV6-AC-GFP
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MG211024 representing BC070445 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCTCGCTGGACCTACCGTACCGCTGCCCGCGCTGCGGGGAGCATAAGCGCTTCCGGAGCCTATCGT CGCTGCGCGCGCACCTGGAGTACAGCCACACCTACGAGACGCTCTACATCCTTTCCAAGACCAACAGCAT CTGCGACGGCGCGGCCGCCGCTGCAGCCGCTGCCGCCGCAGCCTCTGGATTCCCGCTGGCGCCTGAGCCC GCCGCCTTGCTGGCTGTCCCCGGGGCCCGGCGTGAGGTCTTTGAGAGCACGTCCTTCCAGGGCAAGGAAC AGGCGACCGGGCCCTCGCCCGCGGGGCCGCACCTGCTGCACCATCATCACCACCACGCGCCACTGGCGCA CTTTCCCGCTGACCTGGTGCCCGCTAGCCTGCCCTGCGAGGAGCTGGCTGAGCCGGGGCTCGTGCCCGCT GCGCGCTATGCGCTACGCGAAATCGAAATCCCACTCGGCGAGCTGTTTGCGCGCAAGTCCGTGGCATCCT CTGCCTGCTCGACGCCGCCACCCGGACCTGGCCCTGGTCCTTGTTCCGGGCCCTCCTCTGCATCGCCGGC GTCGCCATCACCTGCGGACGTCGCCTACGAAGAGGGCCTGGCACGCCTTAAGATCCGCGCCCTGGAGAAG CTGGAGGTGGACCGGAGACTAGAGCGGCTGAGCGAGGAGGTGGAGCAGAAGATCGCGGGCCAGGTGGGTC GGCTACAGGCGGAGCTGGAGCGCAAGGCAGCAGAGCTAGAAACTGCAAGACAGGAGAGCGCGCGCCTGGG ACGCGAGAAGGAGGAGCTGGAGGAGCGCGCCTCTGAGCTCTCACGCCAAGTGGACGTGAGCGTGGAACTG CTGGCTTCACTCAAGCAGGACCTGGTGCATAAGGAACAGGAGCTGAGCCGCAAGCAGCAGGAGGTGGTGC AGATCGACCAGTTCCTGAAGGAGACGGCAGCTCGGGAGGCCAGTGCCAAGCTGCGGCTGCAACAGTTCAT CGAGGAGCTCCTGGAGCGTGCAGACAGGGCCGAGCGCCAGTTGCAGGTCATCAGCAGCAGCTGTGGCAGC ACACCCAGTGCCAGCCTGGGCAGAGGAGGTGGGGGCAGTGCCTCGGGGCCTGGGGTGAGAGGCCCCGGCA GAATGCGAGAACATCATGCAGGCTCAGCTGTGCCAAGCACATACGCCGTATCACGGCATGGCTCCTCTCC CAGCACAGGGGCCTCCAGTCGTGTGCCAGCTGCATCCCAGAGCTCAGGCTGCTATGACAGTGACAGTCTG GAGCTGCCCCGGCCAGAGGAAGGGCCCTCGGAGGACAGTGGCCCTGGGGGCTTGGGTTCACGGGCCCAGG CTACCAACGGTGGTTCAGAGCGGTCCCAGGCCCCTCGTAGTTCAGGCCTAAGACGACAGGCCATCCAGAA CTGGCAACGCCGACCTCGTCGTCACAGCACCGAGGGGGAGGAGGGTGACGTTTCGGATGTGGGCTCCCGA ACTACTGAGTCAGAGGCTGAAGGACCCTCAGATGTCCCACGCCCTGGACCTGCTGTGGCTGGCCCCTTGA ACAGCTGCCGGCTCTCTGCACGTCCTGAAGGAGGCAGCGGGCGAGGCCGTCGGGTGGAGAGAGGTAGCCC CTCACGCTCCAATGAGGTCATCAGCCCAGAAATCCTCAAGATGCGAGCTGCCCTCTTCTGTATCTTCACC TACCTGGATACCCGGACACTGCTCCATGCAGCAGAGGTCTGTCGGGACTGGCGCTTTGTGGCCCGCCATC CCGCTGTCTGGACGAGAGTGCTACTTGAGAATGCCCGAGTCTGTTCCAAGTTCCTGGCGATGTTGGCTCA GTGGTGCACCCAGGCTCACTCGTTAACATTACAGAACCTGAAGCCCCGGCAGCGGGGGAAGAAGGAAAGC AAGGAGGAGTATGCCCGCAGCACCCGGGGCTGCCTTGAAGCAGGGCTGGAGTCCCTGCTGAAGGCAGCTG GTGGGAACCTGCTGATCCTGCGAATCTCCCACTGCCCCAACATCCTCACTGACCGGTCCCTCTGGCTGGC CAGCTGCTACTGCCGTGCCCTGCAGGCTGTCACCTACAGGAGTGCCACGGACCCCGTCGGCCATGAGGTG ATCTGGGCCCTGGGTGCAGGCTGCAGAGACATTGTCTCCCTCCAGGTGGCGCCACTTCACCCCTGCCAGC AGCCCACCCGGTTCAGCAACCGTTGCCTGCAGATGATTGGACGCTGTTGGCCCCACCTCCGGGCCCTGGG CGTAGGTGGTGCCGGCTGTGGGGTACAGGGCCTGGCATCACTTGCAAGAAACTGTATGCGGCTGCAGGTC CTGGAGCTGGACCACGTGTCAGAGATTACCCAGGAGGTGGCGGCTGAGGTCTGCCGGGAAGGCCTGAAGG GACTGGAGATGCTGGTGCTCACAGCCACCCCTGTCACCCCTAAGGCCCTGCTTCATTTTAACAGCATCTG CCGGAACCTTAAATCCATTGTGGTGCAGATTGGGATTGCAGATTACTTCAAAGAGCCCAGCAGTCCCGAG GCCCAGAAGCTGTTTGAGGACATGGTGACAAAACTCCAGGCCCTGCGTCGGAGGCCTGGCTTCTCGAAGA TTCTGCACATCAAGGTAGAAGGTGGTTGC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA Protein Sequence (showhide) >MG211024 representing BC070445 Red=Cloning site Green=Tags(s) MASLDLPYRCPRCGEHKRFRSLSSLRAHLEYSHTYETLYILSKTNSICDGAAAAAAAAAAASGFPLAPEP AALLAVPGARREVFESTSFQGKEQATGPSPAGPHLLHHHHHHAPLAHFPADLVPASLPCEELAEPGLVPA ARYALREIEIPLGELFARKSVASSACSTPPPGPGPGPCSGPSSASPASPSPADVAYEEGLARLKIRALEK LEVDRRLERLSEEVEQKIAGQVGRLQAELERKAAELETARQESARLGREKEELEERASELSRQVDVSVEL LASLKQDLVHKEQELSRKQQEVVQIDQFLKETAAREASAKLRLQQFIEELLERADRAERQLQVISSSCGS TPSASLGRGGGGSASGPGVRGPGRMREHHAGSAVPSTYAVSRHGSSPSTGASSRVPAASQSSGCYDSDSL ELPRPEEGPSEDSGPGGLGSRAQATNGGSERSQAPRSSGLRRQAIQNWQRRPRRHSTEGEEGDVSDVGSR TTESEAEGPSDVPRPGPAVAGPLNSCRLSARPEGGSGRGRRVERGSPSRSNEVISPEILKMRAALFCIFT YLDTRTLLHAAEVCRDWRFVARHPAVWTRVLLENARVCSKFLAMLAQWCTQAHSLTLQNLKPRQRGKKES KEEYARSTRGCLEAGLESLLKAAGGNLLILRISHCPNILTDRSLWLASCYCRALQAVTYRSATDPVGHEV IWALGAGCRDIVSLQVAPLHPCQQPTRFSNRCLQMIGRCWPHLRALGVGGAGCGVQGLASLARNCMRLQV LELDHVSEITQEVAAEVCREGLKGLEMLVLTATPVTPKALLHFNSICRNLKSIVVQIGIADYFKEPSSPE AQKLFEDMVTKLQALRRRPGFSKILHIKVEGGC TRTRPLE - GFP Tag - V
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene
ACCN	BC070445
ORF Size	2619 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC070445, AAH70445
RefSeq Size	5557 bp
RefSeq ORF	2621 bp
Locus ID	330369
Gene Summary	Substrate-recognition component of the SCF (SKP1-CUL1-F-box protein)-type E3 ubiquitin ligase complex.[UniProtKB/Swiss-Prot Function]

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