Pde6c (BC027050) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	TurboGFP
Synonyms	cpfl1
Vector	pCMV6-AC-GFP
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MG210896 representing BC027050 Red=Cloning site Blue=ORF Green=Tags(s) GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGGCGCGCCC ATGGGTGAGATCAGCCAAGAGGCCGTGGAGCGCTACCTGGAGAAGAACCCTTGTTTTGCCAAAGAGTATT TTGACAAGAAACTACGGGTGGAGGCACTGGGGGTGATCTTCAAAAACAGCCATGCAGGGGTCCAGACCGG CTTGTCGCTCCCTGAGATGACACAGGTGGAGGAGTCGGCCGTGTGCCTGGAGCTACTGCAGTGTATGCAG GACGAGGCCGGAAGCGCGGAGCAGATGGCTCACCGGGCACTGCAGAGGCTCGCTCAACTTCTGCAGGCTG ATTGCTGCAGTATGTTCTCGTGCAGAGCAAGAAACGGCATACCAGAGGTGGCCTCCAGGCTGCTGAACGT CACCCCAACTTCTAAGTTTGAGGACAACCTCGTGGCCCCTGACAGAGAAGTTGTATTTCCGTTGGACATT GGGATAGTTGGCTGGGTCGCTCATGTGAAGAAAGCCCTTAATGTCTCAGATGTCAAAAAGAACAGCCATT TTTCTGACTTTATGGACAAGCAAACTGGATATGTCACCAGGAATCTGCTAGCAGTCCCGATCGTGGCAGG CAAGGAGGTCCTTGCTGTGGTCATGGCAGTGAACAAAATCAGCGCTCCCGAGTTTTCCAAACAGGATGAG GAGGTCTTTTCTAAGTACCTCAGCTTTGTTGCTGTCGCACTAAGGCTGCAGCACACCAGTTACCTGTACA GTGTGGAGTCCCGGAGAAGCCAGATCCTTATGTGGTCAGCCAATAAAGTATTCGAAGAGCTCACTGATGT TGAGCGACAGTTTCACAAGGCACTGTACACCATCAGGACGTACCTGAACTGCGATCGCTACTCCATTGGT CTTCTGGACATGACCAAGGAGAAGGAATTTTACGATGAGTGGCCAATCAAGCTTGGAGAAGTTGAGCCTT ATAAAGGACCAAAGACTCCAGATGGCAGGGAAATCATCTTCTATAAAATCATCGATTACATTTTACATGG AAAAGAAGAGATCAACGTGATTCCGTCACCTCCAGCAGACCACTGGACGCTTGTTAGTGGATTGCCAACA TACGTTGCAGAAAATGGATTTATCTGTAACATGCTGAATGCCCCTGCAGATGAGTACTTCACATTTCAGA AAGGACCGGTAGATGAGACTGGCTGGGTCATTAAAAATGTCTTGTCCCTGCCTATTGTCAACAAGAAAGA AGACATCGTGGGTGTAGCTACGTTTTACAACCGGAAGGATGGGAAACCCTTTGATGAGCATGATGAACAT ATCACTGAGACTTTAACCCAGTTTCTTGGATGGTCTCTTTTAAACACCGATACCTATGAGCGAGTGAACA AGCTGGAAAGCAGAAAGGACATTGCTCAGGAAATGGTTATGAACCTCACAAAAGCCACCCCGGATGAAAT CAGCTCTATTCTGAAGTTTAAAGAGAAGTTAAATGTAGAGGTAATTGAAGAGTGTGAAGAGAGACAGCTC CTGGCCATATTGAAGGAGGACTTGCCGGACCCGAGAACAGCAGACCTGTACGAGTTCTGTTTCAGCGACT TCCCCATTACGGAGCACGAGTTGGTTAAATGCGGGCTGAGGCTGTTTTTGGAGATAAACGTGGTGGAGAA ATTCAAAGTCCCTGTAGAGGTTCTTACAAGGTGGATGTACACAGTCCGGAAAGGGTACCGTCCTGTCACC TACCACAACTGGCGGCACGGATTCAACGTGGGGCAGACCATGTTCACTTTGTTAATGACAGGAAGACTAA AGAAATACTATACAGACCTTGAAGCCTTTGCCATGCTCGCTGCTGCTTTCTGCCATGACATTGACCACAG AGGCACCAATAACTTGTACCAAATGAAGTCCACATCTCCACTTGCAAGGCTTCATGGGACTTCCATTTTA GAGCGACATCACCTGGAATATAGCAAGACTCTCTTGCAGGATGAGAGTTTAAACATCTTCCAGAATCTAA ACAAGCGGCAGTTTGAAACGGTGATCCATTTGTTCGAGGTTGCAATAATAGCAACTGACCTGGCACTCTA TTTCAAGAAGAGAACCATGTTTCAAAAGATCGTTGATACCTGCGAGCAGATGCAAAGTGAAGAAGAAACC ATCAAATACGTAACCTCCGACCCGACCAAAAAGGAAGTCATCATGGCCATGATGATGACTGCCTGTGACC TGTCTGCCATCACCAAGCCCTGGGAGGTGCAGAGTCAGCCTATGATGGACAGAAGCAAAAAGGATGAACT CCCGAAACTTCAAGTGGGATTTATTGATTTTGTTTGTACTTTCGTATATAAGGAGTTCTCTCGGTTTCAT GGAGAGATCACACCCATGCTGAACGGCCTTCAGAATAACAGGGTGGAATGGAAATCCCTGGCGGAGGAGT ACGAGGCGAAGGTGAAGGTGACTGAAGAAGAGGCGGGAAAGCAGGAAGAAGAAGCCTCAGATGGAAAAGC TGCCACAGATTTAGGAGGCAGCGCCGAAGACAAAAAGTCCAAAACATGCTTAATGTTA ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA Protein Sequence (showhide) >MG210896 representing BC027050 Red=Cloning site Green=Tags(s) MGEISQEAVERYLEKNPCFAKEYFDKKLRVEALGVIFKNSHAGVQTGLSLPEMTQVEESAVCLELLQCMQ DEAGSAEQMAHRALQRLAQLLQADCCSMFSCRARNGIPEVASRLLNVTPTSKFEDNLVAPDREVVFPLDI GIVGWVAHVKKALNVSDVKKNSHFSDFMDKQTGYVTRNLLAVPIVAGKEVLAVVMAVNKISAPEFSKQDE EVFSKYLSFVAVALRLQHTSYLYSVESRRSQILMWSANKVFEELTDVERQFHKALYTIRTYLNCDRYSIG LLDMTKEKEFYDEWPIKLGEVEPYKGPKTPDGREIIFYKIIDYILHGKEEINVIPSPPADHWTLVSGLPT YVAENGFICNMLNAPADEYFTFQKGPVDETGWVIKNVLSLPIVNKKEDIVGVATFYNRKDGKPFDEHDEH ITETLTQFLGWSLLNTDTYERVNKLESRKDIAQEMVMNLTKATPDEISSILKFKEKLNVEVIEECEERQL LAILKEDLPDPRTADLYEFCFSDFPITEHELVKCGLRLFLEINVVEKFKVPVEVLTRWMYTVRKGYRPVT YHNWRHGFNVGQTMFTLLMTGRLKKYYTDLEAFAMLAAAFCHDIDHRGTNNLYQMKSTSPLARLHGTSIL ERHHLEYSKTLLQDESLNIFQNLNKRQFETVIHLFEVAIIATDLALYFKKRTMFQKIVDTCEQMQSEEET IKYVTSDPTKKEVIMAMMMTACDLSAITKPWEVQSQPMMDRSKKDELPKLQVGFIDFVCTFVYKEFSRFH GEITPMLNGLQNNRVEWKSLAEEYEAKVKVTEEEAGKQEEEASDGKAATDLGGSAEDKKSKTCLML TRTRPLE - GFP Tag - V
Restriction Sites	AscI-MluI      Cloning Scheme for this gene
ACCN	BC027050
ORF Size	2508 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC027050, AAH27050
RefSeq Size	2901 bp
RefSeq ORF	2510 bp
Locus ID	110855
Gene Summary	As cone-specific cGMP phosphodiesterase, it plays an essential role in light detection and cone phototransduction by rapidly decreasing intracellular levels of cGMP.[UniProtKB/Swiss-Prot Function]

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