Fbxl11 (BC076576) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	TurboGFP
Synonyms	Fbl11, Cxxc8, Fbl7, lalina
Vector	pCMV6-AC-GFP
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MG210889 representing BC076576 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAACCTGAAGAAGAAAGGATTCGGTACAGCCAGAGATTGCGTGGTACCATGCGTCGTCGCTATGAAG ATGATGGCATTTCAGATGATGAAATTGAAGGGAAAAGAACTTTTGACTTGGAAGAGAAGCTCCAAACCAA CAAATATAATGCCAATTTTGTTACTTTTATGGAGGGAAAAGATTTTAATGTAGAGTATATCCAGCGGGGT GGCTTGAGAGACCCTCTCATTTTCAAGAATTCTGATGGACTTGGAATAAAGATGCCGGATCCAGACTTCA CAGTGAATGATGTCAAAATGTGTGTGGGGAGTCGTCGGATGGTGGATGTCATGGATGTGAACACACAGAA GGGGATTGAAATGACCATGGCACAATGGACACGATACTATGAGACTCCAGAGGAAGAGCGAGAAAAACTC TATAATGTTATCAGCCTAGAGTTTAGCCACACCAGGCTTGAGAATATGGTGCAGCGGCCTTCCACGGTGG ATTTCATTGACTGGGTAGATAACATGTGGCCAAGGCACTTGAAAGAAAGTCAGACAGAATCAACAAATGC CATCTTAGAGATGCAGTACCCTAAAGTGCAAAAGTACTGTCTAATGAGTGTTCGAGGCTGCTATACTGAC TTCCATGTGGATTTTGGAGGTACTTCTGTTTGGTATCACATCCACCAAGGTGGAAAGGTCTTCTGGCTCA TCCCCCCTACAGCCCACAACCTGGAGCTGTACGAGAATTGGCTGCTATCAGGGAAACAGGGAGACATCTT TCTGGGTGACCGGGTGTCAGATTGCCAACGAATTGAGCTCAAGCAGGGCTATACCTTCGTTATTCCCTCA GGTTGGATTCATGCTGTGTATACTCCTACAGACACATTAGTGTTTGGAGGCAATTTTTTGCATAGCTTCA ACATCCCCATGCAATTAAAGATATACAGCATTGAAGATCGAACACGGGTTCCAAATAAATTCCGTTACCC ATTTTACTATGAAATGTGTTGGTATGTGTTGGAGCGCTATGTATACTGCATAACCAACCGATCCCACCTA ACTAAGGATTTTCAGAAAGAATCCCTTAGCATGGATATGGAGTTAAATGAGTTGGAGTCTGGAAATGGTG ATGAGGAAGGGGTGGACAGAGAAGCCCGACGCATGAACAATAAGCGATCTGTGCTTACCAGCCCTGTTGC TAATGGAGTGAACCTGGATTACGATGGACTTGGCAAAGCCTGCCGAAGTCTTCCAAGTCTGAAGAAAACT TTGTCTGGAGACTCATCCTCAGACTCTACCCGGGGATCCCACAATGGCCAAGTTTGGGATCCCCAATGTA GCCCTAAAAAGGATAGGCAAGTGCATCTCACCCATTTTGAACTTGAAGGTCTTCGATGTCTTGTAGATAA GTTAGAGTCACTGCCACTGCACAAGAAGTGTGTCCCCACAGGAATAGAAGACGAAGATGCTCTGATTGCT GATGTAAAGATTTTGCTGGAAGAACTTGCCAGTAGCGATCCCAAGTTAGCCCTCACTGGAGTCCCTATAG TACAGTGGCCAAAAAGGGATAAGCTTAAATTCCCTACCAGGCCAAAGGTGAGGGTTCCTACAATTCCCAT CACAAAGCCTCACACCATGAAGCCAGCTCCACGCTTAACACCTGTAAGGCCTGCTGCAGCCTCCCCCATT GTGTCAGGAGCCAGGCGGAGAAGAGTGCGGTGCAGGAAATGCAAAGCTTGTGTGCAAGGAGAATGTGGAG TCTGCCACTACTGCAGGGACATGAAGAAATTTGGTGGACCTGGACGCATGAAGCAATCCTGTGTCCTCCG ACAGTGCTTAGCACCCAGACTGCCTCATTCAGTTACGTGTTCTCTCTGTGGAGAAGTAGATCAGAATGAA GAGACCCAGGACTTTGAAAAGAAACTCATGGAATGCTGCATCTGCAACGAGATAGTTCATCCTGGCTGCC TCCAGATGGATGGAGAGGGGTTGCTGAACGAGGAATTGCCAAATTGCTGGGAGTGTCCAAAGTGTTACCA GGAAGACAGCTCGGACAAAGCCCAGAAGCGGAAAATAGAAGAGAGTGATGAAGAAGCTGTACAAGCCAAA GTCTTACGGCCCCTGAGGAGCTGCGAGGAGCCTCTCACACCCCCGCCTCACTCACCTACTTCCATGCTGC AGCTCATCCACGACCCGGTTTCTCCCCGGGGTATGGTGACTCGGTCATCCCCTGGGGCTGGCCCCAGCGA CCACCACAGTGCCAGCCGTGATGAACGCTTCAAACGGCGGCAGTTGCTGCGGCTACAAGCCACCGAGCGC ACCATGGTACGGGAAAAGGAGAACAATCCCAGCGGCAAAAAGGAGCTGTCTGAAGTTGAGAAAGCCAAGA TCCGGGGATCGTACCTCACTGTCACTCTACAGAGGCCCACCAAAGAGCTCCACGGGACATCCATTGTGCC CAAGCTGCAGGCCATCACGGCCTCCTCTGCCAACCTTCGCCCTAACCCCCCGTGC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA Protein Sequence (showhide) >MG210889 representing BC076576 Red=Cloning site Green=Tags(s) MEPEEERIRYSQRLRGTMRRRYEDDGISDDEIEGKRTFDLEEKLQTNKYNANFVTFMEGKDFNVEYIQRG GLRDPLIFKNSDGLGIKMPDPDFTVNDVKMCVGSRRMVDVMDVNTQKGIEMTMAQWTRYYETPEEEREKL YNVISLEFSHTRLENMVQRPSTVDFIDWVDNMWPRHLKESQTESTNAILEMQYPKVQKYCLMSVRGCYTD FHVDFGGTSVWYHIHQGGKVFWLIPPTAHNLELYENWLLSGKQGDIFLGDRVSDCQRIELKQGYTFVIPS GWIHAVYTPTDTLVFGGNFLHSFNIPMQLKIYSIEDRTRVPNKFRYPFYYEMCWYVLERYVYCITNRSHL TKDFQKESLSMDMELNELESGNGDEEGVDREARRMNNKRSVLTSPVANGVNLDYDGLGKACRSLPSLKKT LSGDSSSDSTRGSHNGQVWDPQCSPKKDRQVHLTHFELEGLRCLVDKLESLPLHKKCVPTGIEDEDALIA DVKILLEELASSDPKLALTGVPIVQWPKRDKLKFPTRPKVRVPTIPITKPHTMKPAPRLTPVRPAAASPI VSGARRRRVRCRKCKACVQGECGVCHYCRDMKKFGGPGRMKQSCVLRQCLAPRLPHSVTCSLCGEVDQNE ETQDFEKKLMECCICNEIVHPGCLQMDGEGLLNEELPNCWECPKCYQEDSSDKAQKRKIEESDEEAVQAK VLRPLRSCEEPLTPPPHSPTSMLQLIHDPVSPRGMVTRSSPGAGPSDHHSASRDERFKRRQLLRLQATER TMVREKENNPSGKKELSEVEKAKIRGSYLTVTLQRPTKELHGTSIVPKLQAITASSANLRPNPPC TRTRPLE - GFP Tag - V
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	BC076576
ORF Size	2505 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC076576, AAH76576
RefSeq Size	5175 bp
RefSeq ORF	2507 bp
Locus ID	225876
Gene Summary	Histone demethylase that specifically demethylates 'Lys-36' of histone H3, thereby playing a central role in histone code. Preferentially demethylates dimethylated H3 'Lys-36' residue while it has weak or no activity for mono- and tri-methylated H3 'Lys-36'. May also recognize and bind to some phosphorylated proteins and promote their ubiquitination and degradation. Required to maintain the heterochromatic state. Associates with centromeres and represses transcription of small non-coding RNAs that are encoded by the clusters of satellite repeats at the centromere. Required to sustain centromeric integrity and genomic stability, particularly during mitosis (By similarity). Regulates circadian gene expression by repressing the transcriptional activator activity of CLOCK-ARNTL/BMAL1 heterodimer and RORA in a catalytically-independent manner (By similarity).[UniProtKB/Swiss-Prot Function]

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