Osbpl3 (BC086485) Mouse Tagged ORF Clone
CAT＃: MG210802
- TrueORF®
Osbpl3 (tGFP-tagged) - Mouse oxysterol binding protein-like 3 (cDNA clone MGC:102333 IMAGE:5344992)
ORF Plasmid: DDK
"BC086485" in other vectors (4)
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CNY 5130.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | ORP3, OSBP3, A530055M08 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG210802 representing BC086485
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGTGACGAGAAGAATCTCGGTGTGTCTCAAAAGTTGGTTTCACCTTCAAGAAGCACAAGTAGTTGCT CCTCCAAGCAAGGAAGCCGGCAGGACAGCTGGGAAGTCGTGGAAGGATTGAGAGGGGAAATGACTTACAC CCAGGAGCCGCCAGTGCAGAAAGGATTTTTGCTGAAAAAGAGGAAGTGGCCCTTAAAAGGCTGGCACAAG AGATTCTTCTGCCTGGAAAAGGGAATCTTGAAATATGCCAAGAGCCAAGCTGATATTGAGCGGGAGAAGT TGCATGGCTGCATTGACGTGGGGCTCTCTGTGATGTCTGTGAAGAAGTCGTCTAAGTGTATCGACCTAGA CACGGAGGAGCACATCTACCACCTGAAGGTGAAGTCAGAAGAACTCTTTGACGAGTGGGTGTCCAAGCTC CGTCACCACAGAATGTACCGGCAGAATGAGATCGCCATGTTTCCCCGGGATGTCAATCACTTTTTCTCAG GGTCCTCTGTCACAGATTCTGCCCCAGGAGTGTTCGAGTCAGTTTCCAGTAGGAAGCGTAGCAGTCTATC AAAGCAGAATTCATTTCCACCCGGAAGCAATTTGTCATTTTCTTGTGGTGGCGACACTCGAGTTCCTTTC TGGCTACAGTCTTCAGAGGACATGGAAAAATGTTCAAAAGACCTGGCACACTGCCATGCCTACCTGCTGG AAATGAGCCAGCTCTTAGAAAGCATGGACGTCCTGCATCGGACATACTCGGCGCCAGCCATCAACGCCAT CCAGGTCCCTAAGCCTTTTTCTGGCCCTGTGAGACTGCACTCCTCCAATCCTAACTTGTCGACGCTGGAC TTTGGAGAAGAGAAGTCTTACTCGGATGGTTCTGAAGCCTCGTCGGAGTTCTCCAAGATGCAGGAGGATC TGTGCCATGTCGCCCACAAAGTTTACTTCGCTTTAAGGTCGGCTTTCAATAGCATATCGGTGGAGAGAGA GAAACTGAAGCAGCTGATGGAGCTGGACACCTCCCCCTCCCCCTCCGCTCAGGTCGTCGGGCTGAAGCAC GCTCTGTCATCCGAAAACTCCAGGGATGAGGGCCGAGCCCTGGTGCACCAGCTCTCCAATGAGAGCAGGC TGTCTATCACCGACTCCCTTTCTGAGTTCTTCGATGCCCAGGAAGTTCTGCTGTCTCCTAGCTCTTCAGA AAACGAGATTTCTGATGATGATTCATACGTCAGTGACATAAGTGATAATCTGTCCTTAGACAACCTCAGT AATGATTTAGACAATGAAAGACAGACCTTGGGGCCTGTTCTCGAGAGTAGTGGGGAAGCCAGATCCAAAA GGAGGACCAGCTTGCCGGCACCGGGTCCCAACACCAGTAGCGTTAGCTTGTGGAGCATCCTGCGCAACAA CATTGGGAAGGACCTGTCCAAGGTGGCCATGCCGGTGGAGCTGAACGAGCCCCTGAACACGCTGCAGCGG CTCTGCGAGGAGCTGGAATACAGCGAGCTTCTGGACAAGGCCTCGAGAATCCCCAGTCCCCTGGAGAGGA TGGTGTACGTAGCAGCCTTCGCCATCTCCGCATATGCATCCAGCTACTTCCGAGCAGGCAGCAAGCCGTT TAACCCGGTCCTTGGAGAAACGTACGAATGCATCCGACAGGACAAGGGTTTCCAGTTTTTTGCCGAGCAG GTCAGCCACCACCCACCTATCTCCGCCTGCCACGCTGAGTCCGGAAACTTTGTTTTCTGGCAAGATGTGA GATGGAAAAATAAATTCTGGGGTAAATCCATGGAAATTGTCCCGATTGGCACAACCCACGTGACTCTGCC TGCTTTTGGAGATCACTTTGAGTGGAACAAAGTAACCTCCTGCATCCACAACATCTTGAGCGGACAGCGA TGGATCGAGCACTATGGCGAAATTGACATCAAGAACCTGAATGACGACTCCTGCCACTGCAAAGTGAACT TCATAAAGGCTAAGTACTGGAGCACTAATGCCCACGAGATCGAAGGCACGGTGTTCGACCGCAGTGGGAA GGCTGTGCACCGGCTGTTTGGGAAATGGCACGAGAGCATCTACTGTGGCGGCGCCTCCTCTTCCACATGC GTCTGGAGAGCAAATCCCATGCCGAAAGGCTATGAGCAGTATTATGGCTTCACACAGTTTGCACTAGAGT TAAATGAAATGGATCCTTTGTCAAGGTCTTTATTACCACCCACCGACACTCGGTTTAGACCAGATCAAAG GCTTCTAGAGGAAGGGAACATAGAGGAGGCTGAGGTTCAGAAGCAGAGGATCGAGAAGCTGCAGAGAGAG CGGCGGAGGGTCTTGGAGGAGAACGGTGTGGAGCACCAGCCCCGGTTTTTCAGGAAATCCAGTGACGATG CTTGGGTAAGCAATGGGACCTACCTGGAACTGAGGAAAGACCTTGGCTTTTCCAAGTTGGACCATCCCGT CTTATGG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG210802 representing BC086485
Red=Cloning site Green=Tags(s) MSDEKNLGVSQKLVSPSRSTSSCSSKQGSRQDSWEVVEGLRGEMTYTQEPPVQKGFLLKKRKWPLKGWHK RFFCLEKGILKYAKSQADIEREKLHGCIDVGLSVMSVKKSSKCIDLDTEEHIYHLKVKSEELFDEWVSKL RHHRMYRQNEIAMFPRDVNHFFSGSSVTDSAPGVFESVSSRKRSSLSKQNSFPPGSNLSFSCGGDTRVPF WLQSSEDMEKCSKDLAHCHAYLLEMSQLLESMDVLHRTYSAPAINAIQVPKPFSGPVRLHSSNPNLSTLD FGEEKSYSDGSEASSEFSKMQEDLCHVAHKVYFALRSAFNSISVEREKLKQLMELDTSPSPSAQVVGLKH ALSSENSRDEGRALVHQLSNESRLSITDSLSEFFDAQEVLLSPSSSENEISDDDSYVSDISDNLSLDNLS NDLDNERQTLGPVLESSGEARSKRRTSLPAPGPNTSSVSLWSILRNNIGKDLSKVAMPVELNEPLNTLQR LCEELEYSELLDKASRIPSPLERMVYVAAFAISAYASSYFRAGSKPFNPVLGETYECIRQDKGFQFFAEQ VSHHPPISACHAESGNFVFWQDVRWKNKFWGKSMEIVPIGTTHVTLPAFGDHFEWNKVTSCIHNILSGQR WIEHYGEIDIKNLNDDSCHCKVNFIKAKYWSTNAHEIEGTVFDRSGKAVHRLFGKWHESIYCGGASSSTC VWRANPMPKGYEQYYGFTQFALELNEMDPLSRSLLPPTDTRFRPDQRLLEEGNIEEAEVQKQRIEKLQRE RRRVLEENGVEHQPRFFRKSSDDAWVSNGTYLELRKDLGFSKLDHPVLW TRTRPLE - GFP Tag - V |
| Restriction Sites | SgfI-MluI Cloning Scheme for this gene |
| ACCN | BC086485 |
| ORF Size | 2457 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | BC086485.1 |
| RefSeq Size | 3233 bp |
| RefSeq ORF | 2459 bp |
| Locus ID | 71720 |
| Gene Summary | Phosphoinositide-binding protein which associates with both cell and endoplasmic reticulum (ER) membranes. Can bind to the ER membrane protein VAPA and recruit VAPA to plasma membrane sites, thus linking these intracellular compartments. The ORP3-VAPA complex stimulates RRAS signaling which in turn attenuates integrin beta-1 (ITGB1) activation at the cell surface. With VAPA, may regulate ER morphology. Has a role in regulation of the actin cytoskeleton, cell polarity and cell adhesion. Binds to phosphoinositides with preference for PI(3,4)P2 and PI(3,4,5)P3. Also binds 25-hydroxycholesterol and cholesterol.[UniProtKB/Swiss-Prot Function] |
Documents
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| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC220828 | Osbpl3 (untagged) - Mouse oxysterol binding protein-like 3 (cDNA clone MGC:102333 IMAGE:5344992), (10ug) |
CNY 9120.00 |
|
| MR210802 | Osbpl3 (Myc-DDK-tagged) - Mouse oxysterol binding protein-like 3 (cDNA clone MGC:102333 IMAGE:5344992) |
CNY 6000.00 |
|
| MR210802L3 | Lenti ORF clone of Osbpl3 (Myc-DDK-tagged) - Mouse oxysterol binding protein-like 3 (cDNA clone MGC:102333 IMAGE:5344992) |
CNY 6560.00 |
|
| MR210802L4 | Lenti ORF clone of Osbpl3 (mGFP-tagged) - Mouse oxysterol binding protein-like 3 (cDNA clone MGC:102333 IMAGE:5344992) |
CNY 6560.00 |
