Tbc1d5 (BC043113) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	TurboGFP
Synonyms	mKIAA0210
Vector	pCMV6-AC-GFP
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MG210792 representing BC043113 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTATAAGTCTGTGTCTGAAACGAGACATCCTCTGCAGTCAGAAGAACAAGAAGTAGGCATTGACCCCT TGTTCAGTTATTCAAATAAAACAAGAGGAGATCTAAGTCAAAATGGAAGAGGATCAAATTCTACCTTAGA CACTGAAGGGACGTTCAATTCATATATGAAAGAATGGGAAGAACTATTTGTAAACAACAATTACTTGGCA ACAGTAAGACAGAAGGGGATTAATGGGCAGCTGAGAAGCAGCAGGTTCCGCAGCATTTGCTGGAAGCTCT TTCTTTGTGTTCTTCCTCAAGATAAAAGTCAGTGGATAAGTAAAATCAAAGAACTAAGAGCCTGGTATAG CAGTATTAAAGAAATACACATCACTAACCCAAGGAAGGCTGCCGGCCAGCAGGATCTGATGATCAACAAC CCCCTATCACAGGATGAAGGAAGTCTTTGGAACAAGTTCTTTCAAGATAAAGAACTACGGTCCATGATTG AGCAAGATGTCAAAAGAACGTTTCCTGAAATGCAGTTTTTCCAACAAGAAAATGTGAGAAAAATTCTTAC AGATGTTCTGTTCTGCTATGCAAGAGAGAACGAGCAGTTGCTTTACAAACAGGGTATGCATGAGCTACTA GCGCCTATCATCTTTACCCTCCACTGTGACCACCAAGCTTTTCTACATGCCAGCGAGTCTGCACAGCCCA GTGAGGAAATGAAAACTCTCCTGAACCCTGAGTATCTGGAGCATGATGCCTATGCAATGTTCTCACAGCT TATGGAAACTGCTGAACCTTGGTTTTCTACGTTTGAGCATGATGGTCAAAAGGGAAAAGAAACTCTGATG GCCCCCATTCCCTTTGCTCGACCACAGGATTTAGGGCCAACAGTTGCCATTGTTACAAAAGTCAACCAGA TCCAGGACCATCTACTGAAGAAACATGATATTGAGCTCTACATGCACCTGAACAGACTAGAAATTGCACC ACAAATATATGGGTTACGATGGGTGCGGCTGCTGTTTGGCAGAGAGTTCCCTCTGCAGGACCTGCTGGTG GTCTGGGATGCCCTGTTTGCGGATAGCCTCAACCTGAGCTTAGTCGACTATGTCTTCACAGCCATGCTAC TCTATATCCGAGATGCTTTGATTTCTAGTAACTATCAGACCTGTCTTGGCCTTCTGATGCATTATCCAAT CATCGGGGACATACACTCACTGATACTGAAGGCTCTGTTCCTTAGAGACCCAAAGAGAAATCCAAGGCCG GCGACTTACCAGTTCCACCCCAACTTAGACTACTACAAAGCACGGGGAGCCGACCTTATGAACAAAAGCC GGACCAATGCCAGAGGTGCTCCCCTGAATATACACAAGGTCTCCAACAGCTTGATTAACTTTGGAAGAAA GCTGATTTCCCCAGCCTCAGCTCCGGGCAGTATGGGTGGCCCTGTACCCGGAAACAACAGCAGCAGTTCC TTCTCTGCTGCCATCCCCACCAGGACCTCCACAGAAGCCCCAAGGCATCATTTGCTGCAGCAGCAACAGC AGCAGCAGCACCAGCAGCAGCAGCAGCAGCAACCACAGCAGCAGCAGCAGCAACACCAGCAGCAACAGCA GCAGCAGAGACTGATGAAATCTGAAAGCATGCCGGTACAGTTGAACAAAGGGCAAAGTTCGAAAACCATC AGTTCATCTCCAAGTATCGAGAGTTTGCCTGGGGGAAGAGAATTCACTGGCTCTCCACCTCCATCCGCTA CTAAAAAGGATTCCTTTTTCAGCAACATCGCACGTTCCCGTTCACACAGCAAAACCATGGGCAGGAAAGA GTCTGAAGAAGAACTAGAGGCTCAGATCTCCTTCCTACAAGGCCAGCTGAATGACCTGGATGCCATGTGC AAGTACTGTGCAAAGGTCATGGACATGCATCTTGTAAACATTCAAGATGTGGTATTACAAGAGAATTTGG AAAAAGAAGATCAAATTCTGGTGTCCTTGGCAGGATTAAAACAGATCAAAGACATTCTGAAAGGTTCCCT GCGCTTCAACCAGAGCCAGCTGGAGGCTGGCGAGAATGAGCAGATTACCATCGCTGATGACCACTACTGC TCCAGTGGCCAGGACCAGGGCAGCCAAGTTCCTAGGGCCGCCAAGCAGGCCTCTTCAGAGATGCCAGGGT GCACCGGTGGAACGACCCCAGATGACTTCATTCTGGTTTCCAAAGAAGATGAGGGGCACCGTGCCAGGGG GGCCTTCTCAGGCCAGGCCCAGCCTCTTCTCACCCTCAGAAGCACATCTGGGAAAAGCCGAGCCCCATCC TGCTCCCCACTGCTGTTCTCAGACCCACTGATGGGCCCAGCCTCAGCCTCAGCCTCCTCCAGCAACCCCA GCTCCAGCCCCGATGATGACAGCAGCAAGGAGTCTGGCTTCACCATTGTGAGCCCCTTGGACATC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA Protein Sequence (showhide) >MG210792 representing BC043113 Red=Cloning site Green=Tags(s) MYKSVSETRHPLQSEEQEVGIDPLFSYSNKTRGDLSQNGRGSNSTLDTEGTFNSYMKEWEELFVNNNYLA TVRQKGINGQLRSSRFRSICWKLFLCVLPQDKSQWISKIKELRAWYSSIKEIHITNPRKAAGQQDLMINN PLSQDEGSLWNKFFQDKELRSMIEQDVKRTFPEMQFFQQENVRKILTDVLFCYARENEQLLYKQGMHELL APIIFTLHCDHQAFLHASESAQPSEEMKTLLNPEYLEHDAYAMFSQLMETAEPWFSTFEHDGQKGKETLM APIPFARPQDLGPTVAIVTKVNQIQDHLLKKHDIELYMHLNRLEIAPQIYGLRWVRLLFGREFPLQDLLV VWDALFADSLNLSLVDYVFTAMLLYIRDALISSNYQTCLGLLMHYPIIGDIHSLILKALFLRDPKRNPRP ATYQFHPNLDYYKARGADLMNKSRTNARGAPLNIHKVSNSLINFGRKLISPASAPGSMGGPVPGNNSSSS FSAAIPTRTSTEAPRHHLLQQQQQQQHQQQQQQQPQQQQQQHQQQQQQQRLMKSESMPVQLNKGQSSKTI SSSPSIESLPGGREFTGSPPPSATKKDSFFSNIARSRSHSKTMGRKESEEELEAQISFLQGQLNDLDAMC KYCAKVMDMHLVNIQDVVLQENLEKEDQILVSLAGLKQIKDILKGSLRFNQSQLEAGENEQITIADDHYC SSGQDQGSQVPRAAKQASSEMPGCTGGTTPDDFILVSKEDEGHRARGAFSGQAQPLLTLRSTSGKSRAPS CSPLLFSDPLMGPASASASSSNPSSSPDDDSSKESGFTIVSPLDI TRTRPLE - GFP Tag - V
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene
ACCN	BC043113
ORF Size	2445 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC043113, AAH43113
RefSeq Size	5597 bp
RefSeq ORF	2447 bp
Locus ID	72238
Gene Summary	May act as a GTPase-activating protein for Rab family protein(s). May act as a GAP for RAB7A. Can displace RAB7A and retromer CSC subcomplex from the endosomal membrane to the cytosol; at least retromer displacement seems to require its catalytic activity. Required for retrograde transport of cargo proteins from endosomes to the trans-Golgi network (TGN); the function seems to require its catalytic activity. Involved in regulation of autophagy. May act as a molecular switch between endosomal and autophagosomal transport and is involved in reprogramming vesicle trafficking upon autophagy induction. Involved in the trafficking of ATG9A upon activation of autophagy. May regulate the recruitment of ATG9A-AP2-containing vesicles to autophagic membranes (By similarity).[UniProtKB/Swiss-Prot Function]

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