Arpp21 (BC053001) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	TurboGFP
Synonyms	ARPP-21, Tarpp
Vector	pCMV6-AC-GFP
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MG210771 representing BC053001 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCTGAGCAAGGAGGACTGACTCCGACCATACTGGAAGAAGGGCAGACGGAGCCAGAGTCTGCCCCAG AAAATGGCATCCTCAAGTCAGAAAGTCTGGATGAGGAGGAGAAGCTGGAACTGCAGCGGCGACTGGCGGC TCAGAACCAAGAGAGGAGAAAATCCAAGTCAGGAGCAGGCAAAGGGAAGCTGACCAGAAGTCTTGCTGTC TGTGAAGAGTCTTCAGCTAGATCTGGAGGGGAAAGTCACCAGGATCAGGAATCAATTCACTTACAGCTTT CCAGTTTCCCCAGCCTGCAAGAGGAGGATAAATCTAGGAAGGATGATTCTGAGAGAGAAAAAGAAAAGGA TAAGAACAGAGAGAAACTCTCTGAGAGACCCAAGATCAGAATGTTATCAAAAGATTGCAGCCAAGAATAC ACAGATTCTACAGGCATAGACTTACATGGGTTTCTAATTAACACGCTGAAGAACAATTCCAGGGACAGGA TGATACTCTTGAAAATGGAGCAGGAAATGATTGATTTCATTGCTGACAGCAATAACCACTATAAAAAGTT CCCCCAGATGTCATCCTATCAAAGGATGCTGGTCCATCGGGTGGCAGCGTACTTTGGATTGGACCACAAC GTGGATCAAACTGGAAAATCTGTCATCATCAACAAGACCAGCAGCACCAGGATACCAGAGCAAAGGTTTT GTGAACATTTAAAAGATGAAAAAAGTGAAGAATCCCAGAAGCGGTTTATCTTGAAGCGAGATAACTCTAG TATTGATAAAGAAGACAATCAGTCAGTTTGCTCACAGGAAAGCCTATTTTTGGACAACAGTAGGCTCCAG GAAGACATGCACATATGCAATGAGACCTATAAGAAAAGACAGCTCTTTCGGGCTCACCGAGACAGCTCAG GAAGAACTTCCGGGAGCCGGCAGAGCAGCTCAGAGACTGAGCTCAGGTGGCCAGACCACCAGCGGGCTTG GAGCAGCACAGATTCGGACAGTTCCAACCGCAATCTGAAGCCCACCATGACCAAGACGGCAAGTTTTGGG GGCATCACGGTGCTGACCAGGGGCGACAGCACGTCCAGTACCAGGAGTGCTGGGAAACTGTCCAAAACAG GTTCTGAGTCTTCCAGCAGTGCGGGCTCCTCAGGATCACTGTCCCGCACCCACCCACAGAGCACGGCCCT GACCTCTAGCGTGGCAGCTGGTTCTCCTGGCTGTATGGCCTATTCAGAGAATGGAATGGGAGGCCAGGTT CCTCCCAGCAGCACCAGCTACATCCTCCTCCCACTTGAAAGTGCGACTGGCATCCCACCCGGAAGCATCC TTCTTAATCCACACACAGGTCAGCCCTTTGTGAACCCGGATGGAACGCCTGCGATATACAACCCTCCCGG AAGTCAGCAGACACTGCGTGGCACTGTTGGTGGGCAGCCCCAGCAACCTCCACAGCAGCAACCTTCGCCT CAGCCTCAGCAGCAGGTCCAGGCATCACAGCCCCAGATGGCAGGGCCACTGGTCACTCAGTCTGTCCAGA GCCTGCAGCCATCTTCCCAGTCTGTGCAATATCCAGCGGTCTCTTTTCCTCCCCAGCATCTCCTGCCTAT GTCGCCAACGCAGCACTTCCCCTTGAGAGAAGAACTGGCTGCCCAGTTCAGCCAGCTGAGCATGAGCCGG CAGTCCTCGGGAGACACTCCTGAACCTCCCTCTGGTACAGTCTACCCAGCCTCTCTCCTGCCACAAACGG CTCAGCCACAGAGTTATGTCATCACTTCTGCAGGCCAGCAGCTCTCTACAGGGGGCTTCTCGGACTCTGG CCCGCCCATCTCCCAGCAGGTCCTGCAGGCCCCGCCCTCTCCCCAGGGATTTGTACAGCAGCCTCCACCT GCACAGATGTCCGTCTATTACTACCCATCTGGTCAGTACCCTACCTCAACCTCACAGCAGTACCGACCCC TCGCCTCAGTCCAGTACAGTGCTCAGCGGAGTCAGCAAATACCACAGACCACACAGCAAGCAGGTTACCA GCCAGTCTTGTCTGGTCAGCAGGGCTTCCAAGGCATGATGGGAGTACAGCAGTCAGCGCACAGCCAGGGT GTGATGAGCAGTCAGCAAGGAGCCCCGGTGCATGGTGTGATGGTCTCCTACCCAACCATGTCTTCTTATC AGGTGCCAATGACCCAAGGCTCTCAAGCAGTGCCCCAGCAGACATACCAACCACCAATCATGCTGCCCAG TCAGGCAGGCCAAGGGTCACTCCCAGCCACCGGAATGCCTGTGTACTGTAACGTCACACCGCCAAACCCT CAGAACAACCTAAGGTTGATGGGTCCACACTGCCCCTCCAGCACGGTTCCTGTCATGTCCGCTAGTTGCA GAACAAACTGTGGGAACGTGAGCAACGCAGGCTGGCAGGTCAAGTTC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA Protein Sequence (showhide) >MG210771 representing BC053001 Red=Cloning site Green=Tags(s) MSEQGGLTPTILEEGQTEPESAPENGILKSESLDEEEKLELQRRLAAQNQERRKSKSGAGKGKLTRSLAV CEESSARSGGESHQDQESIHLQLSSFPSLQEEDKSRKDDSEREKEKDKNREKLSERPKIRMLSKDCSQEY TDSTGIDLHGFLINTLKNNSRDRMILLKMEQEMIDFIADSNNHYKKFPQMSSYQRMLVHRVAAYFGLDHN VDQTGKSVIINKTSSTRIPEQRFCEHLKDEKSEESQKRFILKRDNSSIDKEDNQSVCSQESLFLDNSRLQ EDMHICNETYKKRQLFRAHRDSSGRTSGSRQSSSETELRWPDHQRAWSSTDSDSSNRNLKPTMTKTASFG GITVLTRGDSTSSTRSAGKLSKTGSESSSSAGSSGSLSRTHPQSTALTSSVAAGSPGCMAYSENGMGGQV PPSSTSYILLPLESATGIPPGSILLNPHTGQPFVNPDGTPAIYNPPGSQQTLRGTVGGQPQQPPQQQPSP QPQQQVQASQPQMAGPLVTQSVQSLQPSSQSVQYPAVSFPPQHLLPMSPTQHFPLREELAAQFSQLSMSR QSSGDTPEPPSGTVYPASLLPQTAQPQSYVITSAGQQLSTGGFSDSGPPISQQVLQAPPSPQGFVQQPPP AQMSVYYYPSGQYPTSTSQQYRPLASVQYSAQRSQQIPQTTQQAGYQPVLSGQQGFQGMMGVQQSAHSQG VMSSQQGAPVHGVMVSYPTMSSYQVPMTQGSQAVPQQTYQPPIMLPSQAGQGSLPATGMPVYCNVTPPNP QNNLRLMGPHCPSSTVPVMSASCRTNCGNVSNAGWQVKF TRTRPLE - GFP Tag - V
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	BC053001
ORF Size	2427 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC053001, AAH53001
RefSeq Size	3000 bp
RefSeq ORF	2429 bp
Locus ID	74100
Gene Summary	Isoform 2 may act as a competitive inhibitor of calmodulin-dependent enzymes such as calcineurin in neurons.[UniProtKB/Swiss-Prot Function]

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