Pcdhb9 (BC055319) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	TurboGFP
Synonyms	PcdhbI, Pcdhb4C
Vector	pCMV6-AC-GFP
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MG210685 representing BC055319 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGAAGCTGGGGAGAATTCACCTAAACAGGCAAGTAATGGCATTTATTTTTATGATGGTTTTGGTTC AGGTTTGCAGTGAGCCAACAATTCGATACTCTATCTTAGAGGAAACAGAAAGTGGCTCCTTTGTAGCCCA CCTGGCCAAGGATCTGGGTCTGGGAGCTAGGGAGCTGGCTGCCAGGTCTGCACGGGTGTTGTCTGATGAT TACAAGCAGAGATTACTGCTGGATCCTGAGACTGGAGATTTGCTTCTGAGGGAGAAAGTAGACCGGGAGG AGGTGTGTAGCACTGTGGATCCCTGTGTGCTGCATTTCCAGGTGACTCTTGAAAAGCCAGTGCAATATTT TCAAAGAGAATTATTGATCCAGGACATAAATGATCATGCTCCAGAATTCCCAGATAGAGAACTGCTCTTG AGAATACCAGAGAACAGCCAGCAAGGCACACAATTTTCACTGAATTTAGCCCAGGACTTGGATGTGGGCA GCAATGGGCTTCAACAATACACAGTCAGCCCCAACCCTTATTTTCACGTCCTCACTCAAAATAATAGTAA AGGCAAAAAATACCCAGAGTTGGTGCAGGACAGAGGCCTGGACAGAGAGGAGCAGGCAGAGCTGAGCTTA ACCCTCACAGCTCTGGATGGCGGGTCTCCACCGAGGTCAGGAACAGCCCTGGTTCGAATCCTGATCATGG ATATCAATGACAATGCTCCTGAGTTTGTGAACAGTCCCTATGAGGTGCAGGTCCTAGAGAGCAGTCCTCC AGACTCCCCAGTCTTGACTGTCTTAGCACGGGATGCAGATGCTGGCAACTTTGGGAGAGTTTCCTATGGC TTCTTTCAAGCATCAGATGAAATCCAACAAACATTCTCAATAAACGCAACTAGTGGAGACATGCGATTGA AAAAGAAATTGGATTTTGAAAAAATTAAATCTTACCATGTGGAAATCGAGGCCATAGATGGAGGTGGGCT TTCTGGGAAAGGCTCTGTAACGATAGAGGTGGTGGATGTGAATGACAATGCCCCAGAGCTGACCATATCT TCACTGACCAGCTCTGTCCCAGAAAATGCTCCTGAGACGATCATAAGCATCTTCAGAGTTGGGGACAGGG ATTCCGGAGAGAATGGAAAGATGGTTTGTTCTATTCCAGAGAATCTGCCATTTATTCTGAAATCCACCTT CAAGAACTTCTACACCCTGGTGACAGAGAGCCCTCTGGACAGAGAAAGCAGAGCTGAGTACAATATCACC ATCATGGTCTCTGACATGGGCACACCCAGGCTCACAACATGGCACACCATAAAGGTGCAGGTGTCCGACA TCAACGACAACACCCCCGCCTTCACCCAAACCTCCTACACCATGTTTGTCCGCGAGAACAACAGCCCTGC CCTGCACATAGGCACCATCAGTGCCACAGACTCAGACTCAGGCTCCAATGCCCACATCACCTACTCGCTG CTGCCGCCCCATGATCCGGAACTGGCCCTCTCTTCGTTAATCTCGATTAACGCTGACAATGGGCAGCTGT TTGCACTCAGGGCGCTGGACTATGAGGCCCTGCAGGTCTTCGAGTTCCACGTGGGCGCCACAGATGGAGG CTCACCTCCACTTAGCAGCCAGGCTCTGGTGCGCGTGGTGGTGCTGGATGACAATGACAATGCGCCCTTC GTGCTCTACCCTATGCAGAACGCCTCGGCTCCCTTCACTGAGCTGCTGCCTAGAGCTGCGGAGCCAGGCT ACCTGGTCACCAAGGTGGTTGCAGTGGACCGCGACTCTGGCCAGAATGCCTGGCTATCCTTCCAGTTGCT CAAGGCCACGGAACCTGGGCTGTTCAGCGTGTGGGCGCACAATGGAGAGGTGCGCACCACCAGGTTGTTG AGTGAGCGCGATGTGCCCAAGCACAGGCTACTGCTGGTGGTCAAGGACAATGGAGAGCCTCAGCGCTCTG CCAGTGTCACTCTGCAGGTGCTGTTAGTGGATGGCTTCTCACAATCTTACTTACCTCTTCCAGAGGTAGC GCGGGATCCCGCCCATGAAGATGAGGACGTGCTTACATTGTACCTGGTCATTGCCTTGGCATCTGTGTCT TCTCTCTTCCTCCTGTCTGTGCTGCTGTTTGTGGGGGTAAGACTGTGCAGAAGGGCCAGAGAGGTCTCTC TGGGTGGCTGCTCTATGCCTGGTGAACACTTTCCTGGCCACCTGGTCGATGTCAGTGGAGCAGGGACCCT GTCCCAAAGCTACCAGTATGAGGTGTGTCTGAGGGGCGACTCTGGGACTGGTGAGTTCAAGTTCCTGAAA CCCATGATCCCCAATGCTGGGATAGAAATTATGGAAAGTCCCCACTGCAGGGATAGCTTTGTATTCAAC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA Protein Sequence (showhide) >MG210685 representing BC055319 Red=Cloning site Green=Tags(s) MEKLGRIHLNRQVMAFIFMMVLVQVCSEPTIRYSILEETESGSFVAHLAKDLGLGARELAARSARVLSDD YKQRLLLDPETGDLLLREKVDREEVCSTVDPCVLHFQVTLEKPVQYFQRELLIQDINDHAPEFPDRELLL RIPENSQQGTQFSLNLAQDLDVGSNGLQQYTVSPNPYFHVLTQNNSKGKKYPELVQDRGLDREEQAELSL TLTALDGGSPPRSGTALVRILIMDINDNAPEFVNSPYEVQVLESSPPDSPVLTVLARDADAGNFGRVSYG FFQASDEIQQTFSINATSGDMRLKKKLDFEKIKSYHVEIEAIDGGGLSGKGSVTIEVVDVNDNAPELTIS SLTSSVPENAPETIISIFRVGDRDSGENGKMVCSIPENLPFILKSTFKNFYTLVTESPLDRESRAEYNIT IMVSDMGTPRLTTWHTIKVQVSDINDNTPAFTQTSYTMFVRENNSPALHIGTISATDSDSGSNAHITYSL LPPHDPELALSSLISINADNGQLFALRALDYEALQVFEFHVGATDGGSPPLSSQALVRVVVLDDNDNAPF VLYPMQNASAPFTELLPRAAEPGYLVTKVVAVDRDSGQNAWLSFQLLKATEPGLFSVWAHNGEVRTTRLL SERDVPKHRLLLVVKDNGEPQRSASVTLQVLLVDGFSQSYLPLPEVARDPAHEDEDVLTLYLVIALASVS SLFLLSVLLFVGVRLCRRAREVSLGGCSMPGEHFPGHLVDVSGAGTLSQSYQYEVCLRGDSGTGEFKFLK PMIPNAGIEIMESPHCRDSFVFN TRTRPLE - GFP Tag - V
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene
ACCN	BC055319
ORF Size	2379 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC055319, AAH55319
RefSeq Size	3087 bp
RefSeq ORF	2381 bp
Locus ID	93880

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