Gramd1b (BC070451) Mouse Tagged ORF Clone
CAT＃: MG210355
- TrueORF®
Gramd1b (tGFP-tagged) - Mouse GRAM domain containing 1B (cDNA clone MGC:99432 IMAGE:30616509)
ORF Plasmid: DDK
"BC070451" in other vectors (4)
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CNY 4560.00
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Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | KIAA1201, mKIAA1201, 3222402H23 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG210355 representing BC070451
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCTTCCAGCACTGCCAGTAACTCCAACCGCAGCACGCCAGCCTGCTCTCCCATCCTCCGGAAGCGGT CCCGCTCACCAACACCACAGAACCAGGACGGAGACACCATGGTGGAGAAGGGCTCAGATCACTCCTCGGA CAAGTCCCCGTCCACCCCGGAGCAGGGTGTGCAACGCAGTTGCTCCTCACAGTCTGGTCGGAGCGGTGGT AAAAATTCCAAGAAAAGCCAGAGTTGGTACAATGTGCTAAGCCCCACTTACAAGCAGAGAAATGAAGACT TCAGAAAGCTCTTTAAGCAACTTCCAGACACGGAGCGCCTCATTGTTGATTACTCTTGTGCACTCCAAAG AGACATTCTTCTTCAGGGCCGACTCTACCTCTCAGAAAATTGGATCTGCTTCTACAGCAACATCTTCCGC TGGGAAACCCTGCTGACAGTCCGTTTGAAAGATATCTGCTCCATGACTAAAGAGAAAACAGCTCGCCTCA TTCCCAATGCCATCCAAGTCTGCACTGATTCAGAAAAGCACTTTTTTACTTCCTTTGGAGCCCGGGACAG GACGTACATGATGATGTTCCGGCTCTGGCAGAATGCTCTCCTTGAAAAGCCCCTATGTCCCAAGGAGCTC TGGCACTTTGTCCACCAGTGCTACGGGAACGAGCTGGGCCTAACCAGTGATGATGAGGACTACGTGCCTC CAGATGATGACTTCAACACAATGGGCTACTGTGAGGAGATCCCCATAGAAGAGAATGAAGTGAATGACAG CTCATCCAAAAGTAGCATCGAGACCAAGCCGGATGCCAGCCCACAGCTGCCCAAGAAGTCCATTACCAAC AGCACACTGACGTCTACGGGAAGCAGTGAAGCCCCTGTTTCGTTTGATGGCTTGCCATTGGAGGAGGAGG TGATGGAGGGGGATGGTTCCCTAGAGAAGGAGCTTGCCATTGACAACATCATAGGGGAGAAGATCGAGAT CATGGCGCCTGTGACCTCCCCTTCGCTGGACTTCAATGACAATGAGGATATCCCCACCGAGCTCAGTGAT TCTTCAGATACCCACGATGAAGGAGAGGTCCAGGCCTTCTATGAGGACCTGAGTGGAAGACAATATGTGA ATGAGGTCTTCAACTTCAGCGTGGACAAACTCTATGACCTGCTGTTCACCAACTCACCCTTCCTGCGGGA TTTCATGGAGCAGCGGCGTTTCTCTGATATCATCTTCCATCCATGGAAAAAGGAGGAGAATGGAAACCAG AGTCGAGTGATTCTCTACACTATCACCCTCACTAATCCTCTGGCTCCCAAAACAGCCACTGTCAGGGAGA CACAGACCATGTACAAAGCGAGTCAGGAGAGCGAATGCTACGTGATAGATGCTGAAGTCCTCACCCACGA TGTGCCGTACCATGACTACTTCTACACCATCAATCGCTACACACTCACCAGAGTGGCCCGGAACAAGAGC AGACTCAGGGTCTCCACAGAGCTCCGCTATCGAAAACAGCCCTGGGGGTTTGTGAAAACGTTCATCGAGA AGAACTTCTGGAGTGGACTGGAGGACTACTTCCGTCATTTAGAGACTGAACTAACCAAAACAGAGAGTAC CTACTTGGCTGAGATACACAGGCAGTCACCCAAGGAGAAGGCTAGCAAGTCTTCAGCAGTCCGGAGGAGG AAGCGTCCCCATGCCCACCTGCGGGTGCCTCACCTGGAAGAAGTGATGAGTCCTGTCACCACACCCACTG ATGAAGATGTGGGCCACAGGATCAAGCACGTGGCAGGTTCCACGCAGACACGGCACATCCCGGAGGACAC TCCTGATGGTTTTCACCTACAAAGTGTGTCCAAGCTGCTGCTGGTTATCAGCTGTGTGATCTGTTTCAGT CTGGTGCTGCTGGTTGTCCTTAACATGATGCTCTTCTACAAGCTGTGGATGCTGGAATACACCACCCAGA CCCTCACTGCCTGGCAGGGTCTCAGGCTCCAGGAAAGGTTACCCCAGTCTCAGACAGAATGGGCCCAGCT ATTAGAATCCCAACAGAAGTACCACGACACCGAGCTCCAGAAGTGGAGGGAAATCATCAAATCCTCAGTG TTGCTCCTGGACCAGATGAAGGACTCGCTTATCAATCTTCAGAATGGTATCAGGTCCCGAGACTACACAG CTGAGAGCGACGAGAAGAGGAACCGCTATCAT ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG210355 representing BC070451
Red=Cloning site Green=Tags(s) MASSTASNSNRSTPACSPILRKRSRSPTPQNQDGDTMVEKGSDHSSDKSPSTPEQGVQRSCSSQSGRSGG KNSKKSQSWYNVLSPTYKQRNEDFRKLFKQLPDTERLIVDYSCALQRDILLQGRLYLSENWICFYSNIFR WETLLTVRLKDICSMTKEKTARLIPNAIQVCTDSEKHFFTSFGARDRTYMMMFRLWQNALLEKPLCPKEL WHFVHQCYGNELGLTSDDEDYVPPDDDFNTMGYCEEIPIEENEVNDSSSKSSIETKPDASPQLPKKSITN STLTSTGSSEAPVSFDGLPLEEEVMEGDGSLEKELAIDNIIGEKIEIMAPVTSPSLDFNDNEDIPTELSD SSDTHDEGEVQAFYEDLSGRQYVNEVFNFSVDKLYDLLFTNSPFLRDFMEQRRFSDIIFHPWKKEENGNQ SRVILYTITLTNPLAPKTATVRETQTMYKASQESECYVIDAEVLTHDVPYHDYFYTINRYTLTRVARNKS RLRVSTELRYRKQPWGFVKTFIEKNFWSGLEDYFRHLETELTKTESTYLAEIHRQSPKEKASKSSAVRRR KRPHAHLRVPHLEEVMSPVTTPTDEDVGHRIKHVAGSTQTRHIPEDTPDGFHLQSVSKLLLVISCVICFS LVLLVVLNMMLFYKLWMLEYTTQTLTAWQGLRLQERLPQSQTEWAQLLESQQKYHDTELQKWREIIKSSV LLLDQMKDSLINLQNGIRSRDYTAESDEKRNRYH TRTRPLE - GFP Tag - V |
| Restriction Sites | SgfI-MluI Cloning Scheme for this gene |
| ACCN | BC070451 |
| ORF Size | 2202 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | BC070451, AAH70451 |
| RefSeq Size | 7363 bp |
| RefSeq ORF | 2204 bp |
| Locus ID | 235283 |
| Gene Summary | Cholesterol transporter that mediates non-vesicular transport of cholesterol from the plasma membrane (PM) to the endoplasmic reticulum (ER) (PubMed:30220461). Contains unique domains for binding cholesterol and the PM, thereby serving as a molecular bridge for the transfer of cholesterol from the PM to the ER (PubMed:30220461). Plays a crucial role in cholesterol homeostasis in the adrenal gland and has the unique ability to localize to the PM based on the level of membrane cholesterol (PubMed:30220461). In lipid-poor conditions localizes to the ER membrane and in response to excess cholesterol in the PM is recruited to the endoplasmic reticulum-plasma membrane contact sites (EPCS) which is mediated by the GRAM domain (PubMed:30220461). At the EPCS, the sterol-binding VASt/ASTER domain binds to the cholesterol in the PM and facilitates its transfer from the PM to ER (PubMed:30220461).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC220920 | Gramd1b (untagged) - Mouse GRAM domain containing 1B (cDNA clone MGC:99432 IMAGE:30616509), (10ug) |
CNY 9120.00 |
|
| MR210355 | Gramd1b (Myc-DDK-tagged) - Mouse GRAM domain containing 1B (cDNA clone MGC:99432 IMAGE:30616509) |
CNY 5376.00 |
|
| MR210355L1 | Lenti ORF clone of Gramd1b (Myc-DDK-tagged) - Mouse GRAM domain containing 1B (cDNA clone MGC:99432 IMAGE:30616509) |
CNY 6080.00 |
|
| MR210355L2 | Lenti ORF clone of Gramd1b (mGFP-tagged) - Mouse GRAM domain containing 1B (cDNA clone MGC:99432 IMAGE:30616509) |
CNY 6080.00 |
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