Zbtb24 (BC050933) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	TurboGFP
Synonyms	Bif1, Bsg1, ZNF450
Vector	pCMV6-AC-GFP
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MG210198 representing BC050933 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCAGACACAACCCCGGAGCCTTGTGGGCAGCTTATGGTACACTCAGACACTCACAGTGACACTGTGC TGGCCAGTCTGGAGGATCAAAGGAAGAAGGGCTTTCTTTGTGATATTACTTTAATCGTGGAGAATGTGCA TTTCCGAGCCCATAAAGCCTTGCTGGCCGCCAGTAGCGAGTATTTCTCAATGATGTTTGCGGAAGAGGGC GAGATTGGCCAGTCTATCTACATGCTGGAAGGCATGGTTGCAGACACCTTTGGCATCCTGCTGGAATTTA TCTATACAGGTTATCTACATGCCAGTGAGAAAACGACAGAGCAGATCCTGGCTACTGCTCAGTTCTTAAA AGTCTATGACCTGGTAAAGGCATACGCAGACTTCCAGGATAACCATAGCGCCCCAAAGCCACCAGCTTTG AACTGCACTGGGACGCCAGTGGTGGTTATTTCCAATAAGAAGAACGATCCTCTGAAACGGAAACGGGGAA GACCAAGAAAAGCCAATGGTTTGCAGGAAGGGAGATCAGAACTGGCTGCAGAGGGAGAGCTGCAGCTGAG AGTGAACAATTCAGTTCAGAATAGACAAAACTTTGTGTTTAAAGAAGAAGACAGTGTCAAGCTGAGTGAG CAGACTCCAGAAGACAAGGAATCAGAGCCTGCTGGTGAGCCAGGAAGCGTGGAGGAGGTGCCTGCTGAAA AGGACGAGAACTTTGACCCCAAGGCTGGGGATGGACAGGAGAGCCAGAGTAGGTGCAGCAGGCGGAGAAT CCGCAGGTCTGTCAAGCTCAAAGACTACAAGCTTCTTGGAGATGAAGATGACCAGAGTACTGCCAAGAGG CTATGTGGAAGAAAAAAGCGCTCCAGCGGTCCCGAGGCCCGGTGTAAAGACTGCGACAGAGTCTTTAAAT ATAGTCACTTCTTAGCAATCCACCAGAGACGCCACACGGGGGAACGACCCTTCAAATGTAATGAGTGTGG GAAGGGCTTTGCTCAGAAGCACTCCTTGCAGGTCCATACCAGGATGCACACGGGGGAGCGGCCATACACC TGCACCGTGTGCGGCAAGGCGCTGACCACCAAGCACTCCCTGCTGGAGCACATGAGCCTGCACTCAGGAC AGAAGTCTTTTACCTGTGATCAGTGTGGAAAATATTTCAGCCAGAAAAGACAGCTAAAGAGCCATTATCG TGTCCATACAGGCCACTCACTACCAGAATGCAGCCACTGCCACCGAAAGTTTATGGACGTGTCCCAGCTG AAGAAGCACCTGCGGACACACACAGGCGAGAAGCCATTTACTTGTGAAATCTGTGGGAAATCTTTCACAG CAAAGAGCTCCCTTCAGACCCACATCAGGATCCACCGAGGAGAAAAGCCATACTCCTGCAGCATCTGTGG CAAATGCTTCTCTGACTCCAGTGCCAAGAGGAGACACTGCATCCTGCATACGGGCAAGAAGCCCTTCTCC TGCCCCGAGTGTGGCTTACAGTTCGCTCGCCTAGACAATTTGAAGGCACACTTGAAAATCCACAGCAAAG AGAAGCACACGGCAGACTCCAGCAGTGTTTCTGGCAGTAATGTGGACGAGGGCAGGAACATTCTTCAGCT GCAACCATACCAGCTCTCCACGTCGGGAGAGCAGGAGATTCAGCTTCTTGTCACCGACTCTGTGCATAAC ATTAACTTCATGCCAGGTCCCAGCCAGGGGGTCAGCATCGTGGCTGCCGAGAGCCCCCAGAGCATGGCCA CAGACCCGGCTGCCAACATCACCCTGCTCACACAGCAGCCTGAGCAGCTGCAGGGCCTGATCCTTTCCGC TCAGCAGGAGCAGGCAGAGCACATTCAGAGCCTCAGTGTGATTGGAGGCCAGATGGAGTCCTCCCAGACT GAGCCAGTGCATGTCATCACACTTTCCAAGGAAACGCTGGAGCATCTCCACGCCCACCAGGAGCAAACAA CCAGCTCAGTACCTGCAGCTGACACAGGAGCCCGAGCCACCCCTGTCCCCTCCACTAGGCCAGGAGCAGA GCTGACCCAAGCGCCTCTGGCCGTTCCACTAGACCCTTCCCCTGGAGCAACAGTGGCAGGGTGGCCTTTT GGGCCATCATCTTACAGATCCTTAAAGATG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA Protein Sequence (showhide) >MG210198 representing BC050933 Red=Cloning site Green=Tags(s) MADTTPEPCGQLMVHSDTHSDTVLASLEDQRKKGFLCDITLIVENVHFRAHKALLAASSEYFSMMFAEEG EIGQSIYMLEGMVADTFGILLEFIYTGYLHASEKTTEQILATAQFLKVYDLVKAYADFQDNHSAPKPPAL NCTGTPVVVISNKKNDPLKRKRGRPRKANGLQEGRSELAAEGELQLRVNNSVQNRQNFVFKEEDSVKLSE QTPEDKESEPAGEPGSVEEVPAEKDENFDPKAGDGQESQSRCSRRRIRRSVKLKDYKLLGDEDDQSTAKR LCGRKKRSSGPEARCKDCDRVFKYSHFLAIHQRRHTGERPFKCNECGKGFAQKHSLQVHTRMHTGERPYT CTVCGKALTTKHSLLEHMSLHSGQKSFTCDQCGKYFSQKRQLKSHYRVHTGHSLPECSHCHRKFMDVSQL KKHLRTHTGEKPFTCEICGKSFTAKSSLQTHIRIHRGEKPYSCSICGKCFSDSSAKRRHCILHTGKKPFS CPECGLQFARLDNLKAHLKIHSKEKHTADSSSVSGSNVDEGRNILQLQPYQLSTSGEQEIQLLVTDSVHN INFMPGPSQGVSIVAAESPQSMATDPAANITLLTQQPEQLQGLILSAQQEQAEHIQSLSVIGGQMESSQT EPVHVITLSKETLEHLHAHQEQTTSSVPAADTGARATPVPSTRPGAELTQAPLAVPLDPSPGATVAGWPF GPSSYRSLKM TRTRPLE - GFP Tag - V
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	BC050933
ORF Size	2130 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC050933, AAH50933
RefSeq Size	2850 bp
RefSeq ORF	2132 bp
Locus ID	268294
Gene Summary	This gene encodes a protein containing eight C2H2-type zinc fingers and a BTB domain. Expression of this gene is induced by bone morphogenetic protein-2 signaling. Mutation of the related gene in humans causes immunodeficiency-centromeric instability-facial anomalies syndrome-2. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2013]

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