Ints10 (BC031923) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	TurboGFP
Synonyms	4921521J11Rik; AI462004
Vector	pCMV6-AC-GFP
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MG210189 representing BC031923 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCGGCCCAGGGCGACTGTGAGTTTCTGGTGCAGCGAGCCCGGGAACTAGTGCCCCAGGACCTGTGGG CAGCCAAGGCATGGCTGATCACGGCCCGCAGCCTCTACCCGGCCGACTTCAACATCCAGTATGAAATGTA CACCATCGAACGGAATGCCGAGAGGACTGCCACCGCAGGGAGGTTGCTGTATGACATGTTTGTGAATTTC CCGGACCAGCCGGTGGTTTGGAGAGAAATCAGCATCATCACGTCAGCGTTGAGGAACGACTCACAAGATA AGCAGACCCAATTCTTACGGAGTTTATTTGAAACCCTTCCCGGTCGGGTCCAGTGTGAAATGTTGTTGAA GGTGACAGAGCAATGCTTCAACACGCTGGAACGATCAGAAATGTTGCTGCTGCTGCTGAGGCGTTTCCCT GAGACGGTGGTGCAACACGGGGTTGGTCTCGGGGAGGCCCTGCTGGAAGCTGAAACCATTGAAGAACAAG ACTCTCCTGTTAACTGCTTTAGAAAGCTATTTGTTTGTGATGTCCTTCCTCTAATAATCAACAACCATGA TGTCCGCTTGCCCGCCAATCTCTTATATAAATACTTGAACAAAGCAGCTGAATTTTACATCAACTATGTC ACTCGGTCCACACAGTCAGAAAATCAGCATCAAGGTGCACAGGATACATCTGATTTAATGTCGCCTAGCA AGCGTAGCTCTCAGAAGTACATAATAGAAGGACTGACTGAGAAATCATCCCACATCGTCGACCCCTGGGA GAGGTTGTTTAAGATTCTGAATGTTGTTGGAATGAGATGCGAATGGCAGATGGATAAAGGGAGACGAAGC TGCAGTGACCTGCTGCACCGGATGAAGGAGCTCTGCAGATACATGAACAGCTTTGACAGCGAAGCTCACA CCAACTATAAAAACCAAGTGCTCTACTCCACGATGCTGGTGTTCTTCAAGAGTGCGTTCCAGTACGTCAG CAGCATACAGCCATCTCTCTTCCAAGGTCCTAACGCCCCAAGCCAGGTTCCACTAGTTCTTCTGGAAGAT GTAGCCAATGTGTATGGTGATGTAGAGATTGATCGCAGTAAACACATACACAAGAAGAGGAAGCTAGCTG AGGGAAGAGAAAAAACCATGAGTTCAGATGACGAAGAGTGTTCAGCGAAAGGCAGAAACCGGCACATTGT TGTCAGCAAAGCTGACCTCAGCAACTCCATCGAAGTACTGGAGAGCTTCAAACTGGCCAGGGAGAGCTGG GAGTTACTCTACTCTCTGGAATTCCTTGACAAAGAATTTACAAGAATTTGCTTGGCTTGGAAGACAGACA CCTGGCTGTGGCTAAGAATCTTCCTCACAGATATGATCATCTACCAGGGTCAATATAAGAAGGCCATAGC CAGTCTGCATCACTTAGCAGCTCTGCAAGGATCCCTTTCTCAGCCACAGATCACAGGACAGGGGACTTTA GAACACCAGAGGGCGCTCATCCAGCTGGCAACGTGTCACTTTGCTCTGGGGGAGTACAGAATGACGTGCG AGAAAGTCCTAGATCTGATGTGTTACATGGTGCTCCCCATACAAGATGGCGGGAAGCCACAGGAAGAGCC GTCCAAAGTAAAGCCCAAGTGTAGAAAAGGTTTGGATCTGAAGCTTCTGCCCTGTACCAGTAAGGCTATC ATGCCATACTGCCTGCACTTGATGCTGGCCTGCTTTAAGCTGAGAGCTTTTACAGACAGCAGAGACGACA TGGCGCTGGGCCATGTGATTGTACTGCTCCAGCAGGAGTGGCCACGGGGAGAGAACCTTTTCCTGAAAGC CATCAGTAAGATTTGCCAGCAAGGAAATTTCCAGTATGAGAACTTCTTCAGCTATGTTACAAACATTGAT ATGCTGGAGGAATTTGCTTATTTAAGAACCCAAGAAGGAGGGAAGATTCATCTGGAATTACTCCCAAATC AAGGAATGCTGATCAAGCACCACACAGTCACTCGAGGCATCACCAAAGGTGTGAAGGAGGACTTCCGCCT GGCCATGGAGCGCCAGGTCTCCCGCTGTGGTGAGAACCTGATGGCGGTACTACATCGCTTCTGCATTAAT GAGAAGATCTTGCTCCTGCAGACTCTGACC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA Protein Sequence (showhide) >MG210189 representing BC031923 Red=Cloning site Green=Tags(s) MSAQGDCEFLVQRARELVPQDLWAAKAWLITARSLYPADFNIQYEMYTIERNAERTATAGRLLYDMFVNF PDQPVVWREISIITSALRNDSQDKQTQFLRSLFETLPGRVQCEMLLKVTEQCFNTLERSEMLLLLLRRFP ETVVQHGVGLGEALLEAETIEEQDSPVNCFRKLFVCDVLPLIINNHDVRLPANLLYKYLNKAAEFYINYV TRSTQSENQHQGAQDTSDLMSPSKRSSQKYIIEGLTEKSSHIVDPWERLFKILNVVGMRCEWQMDKGRRS CSDLLHRMKELCRYMNSFDSEAHTNYKNQVLYSTMLVFFKSAFQYVSSIQPSLFQGPNAPSQVPLVLLED VANVYGDVEIDRSKHIHKKRKLAEGREKTMSSDDEECSAKGRNRHIVVSKADLSNSIEVLESFKLARESW ELLYSLEFLDKEFTRICLAWKTDTWLWLRIFLTDMIIYQGQYKKAIASLHHLAALQGSLSQPQITGQGTL EHQRALIQLATCHFALGEYRMTCEKVLDLMCYMVLPIQDGGKPQEEPSKVKPKCRKGLDLKLLPCTSKAI MPYCLHLMLACFKLRAFTDSRDDMALGHVIVLLQQEWPRGENLFLKAISKICQQGNFQYENFFSYVTNID MLEEFAYLRTQEGGKIHLELLPNQGMLIKHHTVTRGITKGVKEDFRLAMERQVSRCGENLMAVLHRFCIN EKILLLQTLT TRTRPLE - GFP Tag - V
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	BC031923
ORF Size	2130 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC031923, AAH31923
RefSeq Size	2424 bp
RefSeq ORF	2132 bp
Locus ID	70885
Gene Summary	Component of the Integrator (INT) complex, a complex involved in the small nuclear RNAs (snRNA) U1 and U2 transcription and in their 3'-box-dependent processing. The Integrator complex is associated with the C-terminal domain (CTD) of RNA polymerase II largest subunit (POLR2A) and is recruited to the U1 and U2 snRNAs genes. May be not involved in the recruitment of cytoplasmic dynein to the nuclear envelope by different components of the INT complex.[UniProtKB/Swiss-Prot Function]

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