Lrrc4b (BC060263) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	TurboGFP
Synonyms	A830007M12; Lrig4
Vector	pCMV6-AC-GFP
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MG210186 representing BC060263 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGCAGGCCCACATCCGCGGCTCCCCGTGCCCCTTGCTGCCGCCTGGTAGGATGTCCTGGCCCCACG GGGCTCTCTTGCTCCTGTGGCTCTTCTCCCCACCCCTGAGGGCTGGTGGGGGCGGTGTGGCCGTGACCTC AGCCGCTGGAGGAGGCTCCCCGCCAGCCACCTCCTGCCCAGCCGCCTGCTCCTGCAGCAACCAGGCCAGC CGGGTGATCTGCACGCGGAGGGAGCTGGCCGAGGTGCCAGCCAGCATCCCTGTCAACACCCGCTACCTGA ACCTGCAAGAGAACAGCATCCAGGTGATCCGCACGGACACATTCAAGCACCTCCGGCACCTGGAGATCCT GCAGCTGAGCAAGAACCTGGTGCGAAAGATCGAGGTGGGAGCGTTCAATGGGCTGCCCAGTCTCAACACA CTGGAACTCTTCGACAACCGGCTGACAACCGTGCCCACGCAGGCCTTTGAGTACCTGTCCAAGCTCCGGG AGCTGTGGCTGCGCAACAACCCCATCGAAAGCATCCCATCCTATGCCTTCAACCGTGTGCCCTCACTGCG CCGCCTAGACCTGGGCGAGCTGAAGAGGCTGGAGTACATATCTGAGGCGGCCTTCGAGGGGCTGGTGAAC CTGCGCTACCTCAACCTGGGCATGTGCAACCTAAAGGACATCCCCAACCTCACAGCACTTGTGCGCCTGG AGGAGCTGGAGCTGTCCGGGAACCGGCTGGACCTAATTCGCCCTGGCTCCTTCCAGGGCCTCACCAGCCT GCGCAAGCTGTGGCTGATGCATGCCCAAGTGGCCACCATCGAGCGAAACGCCTTCGACGACCTCAAGTCG CTGGAGGAGCTGAACCTGTCCCACAACAACCTGATGTCGCTGCCGCACGACCTTTTCACGCCCCTTCACC GCCTGGAGCGTGTCCACCTGAACCACAACCCCTGGCATTGTAACTGTGATGTGCTGTGGCTCAGCTGGTG GCTGAAGGAGACAGTGCCCAGCAATACCACATGCTGCGCACGCTGCCACGCGCCCGCCGGCCTCAAGGGC CGCTACATTGGCGAGCTAGACCAGTCGCACTTCACCTGCTATGCCCCGGTCATCGTGGAGCCACCCACAG ACCTCAATGTCACCGAGGGCATGGCGGCTGAGCTCAAGTGCCGCACAGGCACATCCATGACGTCGGTCAA CTGGCTGACACCTAACGGCACGCTCATGACGCACGGTTCCTATCGTGTGCGCATTTCAGTGCTGCACGAT GGCACACTCAACTTCACCAATGTCACCGTGCAGGACACGGGCCAGTACACGTGCATGGTGACAAACTCGG CTGGCAACACTACTGCCTCGGCCACGCTTAACGTGTCGGCCGTGGACCCCGTGGCTGCGGGAGGGCCCGG TGGCGGGGGCCCAGGGGGTGGGGGCGGTGCAGGGGGCGCGGGCGGATACACCTACTTCACCACGGTGACC GTGGAGACGCTGGAGACGCAGCCGGGGGAGGAGGCCCAGCAGCCCCGAGGTACTGAGAAGGAGCCCCCGG GGCCCACGACTGATGGCGCGTGGGGCGGCGGCCGGCCTGATGCTGCAGCCCCGGCCTCGGCGTCCACCAC GGCACCGGCCCCGCGCTCCTCGCGGCCCACGGAGAAGGCTTTCACGGTGCCCATCACGGACGTGACGGAG AATGCGCTTAAGGACCTAGACGATGTCATGAAGACCACCAAAATCATCATCGGCTGCTTCGTGGCCATCA CCTTCATGGCCGCCGTGATGCTGGTGGCCTTCTACAAGCTGCGCAAGCAGCACCAGCTGCACAAGCACCA CGGGCCCACGCGCACTGTGGAGATCATCAACGTGGAGGACGAGCTGCCCGCCGCCTCCGCTGTGTCGGTG GCTGCGGCCGCCGCGGTGGCTGGAGGCGCGGGCGTGGGTGGGGACAGCCACCTGGCCCTTCCGGCGCTGG AGCGAGACCACCTCAATCACCACCACTACGTTGCGGCCGCCTTCAAGGCGCACTACGGCGGCAACCCGGG AGGCGGGTGCGGGGCCAAAGGCCCCGGCCTCAACTCCATCCACGAACCTCTGCTCTTCAAGAGCGGCTCC AAGGAGAACGTGCAAGAGACACAAATC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene
ACCN	BC060263
ORF Size	2127 bp
OTI Disclaimer	Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC060263, AAH60263
RefSeq Size	2753 bp
RefSeq ORF	2129 bp
Locus ID	272381
Gene Summary	Synaptic adhesion protein. Regulates the formation of excitatory synapses. The trans-synaptic adhesion between LRRC4B and PTPRF regulates the formation of excitatory synapses in a bidirectional manner (By similarity).[UniProtKB/Swiss-Prot Function]

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