Aplp2 (BC051999) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	TurboGFP
Synonyms	AI790698; APLP-2
Vector	pCMV6-AC-GFP
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MG210177 representing BC051999 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGGCCACCGGGACCGCGGCCGCTGCGGCCACCGGCAAGCTTCTTGTCCTGCTGCTGCTCGGGCTCA CGGCGCCCGCTGCGGCTCTGGCTGGCTACATAGAGGCTCTTGCAGCCAATGCTGGAACAGGCTTCGCTGT TGCTGAGCCTCAGATTGCGATGTTCTGTGGGAAGCTGAATATGCATGTGAACATTCAGACTGGCAAATGG GAGCCTGACCCAACAGGCACCAAGAGCTGCCTTGGAACAAAGGAGGAGGTTCTTCAGTACTGCCAGGAGA TATATCCAGAGCTGCAGATCACAAATGTGATGGAAGCAAACCAGCCAGTCAATATTGATAGTTGGTGCCG AAGGGACAAAAGGCAGTGCAAGAGTCACATTGTTATACCATTCAAGTGTCTTGTGGGTGAATTTGTAAGT GATGTCCTGCTAGTTCCAGATAACTGCCAGTTTTTCCACCAAGAGCGGATGGAGGTGTGTGAGAAGCACC AGCGCTGGCACACGTTAGTCAAGGAGGCATGTCTGACTGAGGGGCTGACCTTATATAGCTATGGCATGCT GCTGCCCTGCGGGGTAGACCAGTTCCATGGCACCGAGTATGTGTGCTGCCCTCAGACAAAGACTGTTGAC TCGGACTCGACTATGTCCAAAGAAGAGGAGGAAGAGGAAGAGGATGAAGAGGACGAAGAGGAAGACTATG ATCTTGATAAAAGTGAATTTCCTACTGAAGCAGATTTGGAAGACTTCACAGAAGCAGCAGCAGATGAGGA AGAAGAGGATGAGGAGGAAGGGGAGGAAGTGGTGGAAGACCGTGACTACTACTATGACCCCTTTAAAGGA GACGATTACAATGAGGAGAATCCAACCGAACCCAGCAGCGAGGGCACCATTTCAGACAAGGAGATTGTTC ACGATGTTAAAGTTCCCCCGACTCCCCTGCCAACCAATGATGTTGATGTGTATTTTGAGACCTCAGCGGA TGATAATGAGCACGCCCGCTTCCAGAAGGCTAAGGAACAGCTGGAAATTCGACATCGAAACCGAATGGAC AGGGTAAAGAAGGAATGGGAAGAGGCAGAACTTCAAGCCAAGAACCTCCCCAAGACAGAAAGACAGACCC TCATCCAGCATTTCCAAGCTATGGTTAAAGCTTTAGAGAAAGAAGCAGCCAGTGAGAAGCAGCAGCTTGT GGAAACCCATCTGGCCCGAGTAGAAGCCATGCTGAATGACCGCCGACGCATAGCTCTGGAAAATTACCTG GCTGCCCTGCAGTCTGACCCACCTCGGCCACATCGGATTCTTCAAGCTCTTCGTCGTTACGTCCGTGCTG AGAACAAAGATCGCCTGCATACCATTCGTCATTACCAACACGTGTTGGCTGTTGACCCAGAAAAGGCCGC CCAGATGAAATCCCAGGTGATGACACACCTCCATGTGATTGAAGAAAGAAGGAACCAAAGTCTTTCTCTT CTGTACAAAGTTCCTTATGTTGCTCAAGAAATTCAAGAGGAAATTGATGAGCTCCTTCAGGAACAGCGAG CGGATATGGACCAATTTACCTCCTCCATCTCAGAGAACCCTGTGGATGTCCGGGTGAGCTCTGAGGAGAG TGAGGAGATCCCGCCGTTCCACCCTCTCCATCCCTTCCCATCCTTGTCTGAGAATGAAGACACTCAGCCG GAGTTGTACCACCCAATGAAAAAAGGCTCTGGAATGGCAGAACAAGACGGGGGACTGATTGGTGCAGAAG AAAAAGTGATTAACAGCAAGAATAAAATGGATGAAAATATGGTCATTGACGAGACTCTGGATGTTAAGGA AATGATTTTCAATGCTGAGAGAGTTGGAGGCCTTGAGGAAGAGCCGGAATCGGTGGGACCTTTAAGGGAG GATTTCAGTTTGAGCAGCAATGCCCTTATTGGCTTGCTGGTTATCGCAGTGGCCATTGCTACGGTCATCG TTATCAGCCTGGTGATGCTGAGGAAGAGGCAGTACGGCACCATCAGCCACGGGATTGTGGAGGTTGACCC AATGCTCACCCCAGAAGAGCGTCACCTGAACAAGATGCAGAACCATGGTTATGAAAACCCAACCTACAAA TACCTGGAGCAGATGCAGATT ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA Protein Sequence (showhide) >MG210177 representing BC051999 Red=Cloning site Green=Tags(s) MAATGTAAAAATGKLLVLLLLGLTAPAAALAGYIEALAANAGTGFAVAEPQIAMFCGKLNMHVNIQTGKW EPDPTGTKSCLGTKEEVLQYCQEIYPELQITNVMEANQPVNIDSWCRRDKRQCKSHIVIPFKCLVGEFVS DVLLVPDNCQFFHQERMEVCEKHQRWHTLVKEACLTEGLTLYSYGMLLPCGVDQFHGTEYVCCPQTKTVD SDSTMSKEEEEEEEDEEDEEEDYDLDKSEFPTEADLEDFTEAAADEEEEDEEEGEEVVEDRDYYYDPFKG DDYNEENPTEPSSEGTISDKEIVHDVKVPPTPLPTNDVDVYFETSADDNEHARFQKAKEQLEIRHRNRMD RVKKEWEEAELQAKNLPKTERQTLIQHFQAMVKALEKEAASEKQQLVETHLARVEAMLNDRRRIALENYL AALQSDPPRPHRILQALRRYVRAENKDRLHTIRHYQHVLAVDPEKAAQMKSQVMTHLHVIEERRNQSLSL LYKVPYVAQEIQEEIDELLQEQRADMDQFTSSISENPVDVRVSSEESEEIPPFHPLHPFPSLSENEDTQP ELYHPMKKGSGMAEQDGGLIGAEEKVINSKNKMDENMVIDETLDVKEMIFNAERVGGLEEEPESVGPLRE DFSLSSNALIGLLVIAVAIATVIVISLVMLRKRQYGTISHGIVEVDPMLTPEERHLNKMQNHGYENPTYK YLEQMQI TRTRPLE - GFP Tag - V
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	BC051999
ORF Size	2121 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC051999, AAH51999
RefSeq Size	3525 bp
RefSeq ORF	2123 bp
Locus ID	11804
Gene Summary	May play a role in the regulation of hemostasis. The soluble form may have inhibitory properties towards coagulation factors. May interact with cellular G-protein signaling pathways. May bind to the DNA 5'-GTCACATG-3'(CDEI box). Inhibits trypsin, chymotrypsin, plasmin, factor XIA and plasma and glandular kallikrein (By similarity). Modulates the Cu/Zn nitric oxide-catalyzed autodegradation of GPC1 heparan sulfate side chains in fibroblasts.[UniProtKB/Swiss-Prot Function]

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