Scmh1 (BC034667) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	TurboGFP
Synonyms	AI315320; AI851618; Scml1; Scml3
Vector	pCMV6-AC-GFP
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MG210159 representing BC034667 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCTGGTTTGCTACAGTGTTTTAGCTTGTGAGAGTCTCTGGGACCTTCCCTGCTCCATCATGGGGTCAC CTCTAGGTCATTTTACCTGGGACAAATACCTAAAAGAAACATGTTCAGTCCCAGCGCCTGTCCATTGCTT CAAGCAGTCCTACACACCTCCAAGTAATGAGTTCAAGATCAGCATGAAATTGGAAGCACAGGATCCCAGG AACACCACATCCACCTGTATTGCCACGGTCGTTGGATTGACAGGTGCCCGACTTCGTCTGCGCCTTGATG GCAGTGACAACAAGAATGACTTCTGGAGACTGGTTGACTCCTCTGAAATCCAGCCAATTGGAAACTGTGA GAAGAATGGCGGGATGCTGCAGCCCCCTCTAGGATTTCGGCTGAATGCCTCCTCTTGGCCCATGTTCCTT TTGAAGACACTAAATGGAGCAGAGATGGCTCCCATCAAGATTTTCCATAAGGAGCCACCATCACCTTCCC ACAACTTCTTCAAAATGGGAATGAAGTTAGAAGCTGTAGACAGAAAGAACCCTCATTTCATTTGCCCAGC CACTATTGGAGAAGTTCGAGGCGCAGAAGTGCTAGTCACCTTTGATGGGTGGCGAGGCGCATTTGACTAC TGGTGCCGCTTTGACTCCCGGGACATCTTTCCTGTGGGCTGGTGTTCTTTGACTGGAGATAACCTGCAGC CACCTGGCACCAAAGTTGTGATTCCAAAGAATCCGTCCCCTTCATCTGATGTGAGCACTGAGAAGCCCAG CATCCACAGCACCAAAACTGTCTTGGAGCATCAGCCAGGGCAGAGGGGCCGCAAACCAGGAAAGAAGCGG GGCCGAACACCCAAGATCCTTATTCCCCATCCCACCTCTACCCCATCCAAGTCAGCTGAACCTTTGAAAT TTCCAAAGAAGAGAGGTCCCAAGCCTGGCAGTAAGAGGAAACCTCGGACTTTGCTGAGCCCACCACCCAC CTCACCAACAACCAGCACCCCTGAACCGGACACCAGCACTGTTCCTCAAGATGCTGCCACCGTCCCAAGT TCAGCCATGCAGGCCCCCACAGTTTGTATCTACTTGAACAAGAGCGGCAGCACGGGCCCCCACCTGGATA AGAAGAAGATCCAACAACTCCCTGACCATTTTGGGCCAGCCCGTGCCTCTGTGGTGCTGCAGCAGGCTGT CCAGGCTTGCATTGACTGTGCTTATCACCAGAAAACTGTCTTCAGCTTCCTCAAACAGGGCCACGGCGGT GAAGTCATTTCAGCCGTGTTTGACCGGGAACAGCACACTCTGAACCTCCCAGCAGTCAACAGCATCACCT ATGTCCTCCGTTTCCTGGAGAAGCTCTGCCACAACCTTCGAAGTGACAATCTGTTTGGCAACCAGCCCTT TACACAGACTCACTTATCACTCACTGCCACAGAGTATAATCACAACCACGACAGGTACCTACCAGGTGAA ACCTTTGTCTTGGGGAATAGCCTGGCCCGGTCCTTGGAGACACACTCAGACCTGATGGATTCTGCCTTGA AGCCTGCCAACCTTGTCAGCACATCCCAAAACCTTCGGACTCCTGGCTATCGGCCCTTGCTTCCCTCCTG TGGCCTCCCATTAAGCACTGTCTCTGCTGTGCGTAGGCTCTGCTCTAAGGGAGTGTTAAAAGGAAAAAAG GAAAGAAGGGATGTGGAGTCATTTTGGAAACTAAATCATTCCCCAGGGTCAGATCGACATCTGGAGAGCC GAGATCCCCCTCGCCTGAGTGGCCGGGACCCCTCCTCATGGACAGTGGAGGATGTGATGCAGTTTGTCCG GGAAGCCGATCCTCAGCTTGGATCCCATGCTGACCTCTTCCGAAAACATGAAATCGATGGCAAGGCCCTG CTCCTGCTGCGCAGTGACATGATGATGAAGTACATGGGCCTGAAGCTGGGGCCCGCCCTCAAGCTCTCCT TTCACATTGACCGGCTGAAGCAGGTTTTTTGGAAGAGGGAGACCATCCTCTGGTCCAGAGAGGGCCTCTC CAGAGAAGTGTGGCCTATTTCAGAAGACACTGCCCTAGGGCACTTCTTCTCTGGAATGGACAAAGTATTT GGCTCACTGAGCAAAAGG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA Protein Sequence (showhide) >MG210159 representing BC034667 Red=Cloning site Green=Tags(s) MLVCYSVLACESLWDLPCSIMGSPLGHFTWDKYLKETCSVPAPVHCFKQSYTPPSNEFKISMKLEAQDPR NTTSTCIATVVGLTGARLRLRLDGSDNKNDFWRLVDSSEIQPIGNCEKNGGMLQPPLGFRLNASSWPMFL LKTLNGAEMAPIKIFHKEPPSPSHNFFKMGMKLEAVDRKNPHFICPATIGEVRGAEVLVTFDGWRGAFDY WCRFDSRDIFPVGWCSLTGDNLQPPGTKVVIPKNPSPSSDVSTEKPSIHSTKTVLEHQPGQRGRKPGKKR GRTPKILIPHPTSTPSKSAEPLKFPKKRGPKPGSKRKPRTLLSPPPTSPTTSTPEPDTSTVPQDAATVPS SAMQAPTVCIYLNKSGSTGPHLDKKKIQQLPDHFGPARASVVLQQAVQACIDCAYHQKTVFSFLKQGHGG EVISAVFDREQHTLNLPAVNSITYVLRFLEKLCHNLRSDNLFGNQPFTQTHLSLTATEYNHNHDRYLPGE TFVLGNSLARSLETHSDLMDSALKPANLVSTSQNLRTPGYRPLLPSCGLPLSTVSAVRRLCSKGVLKGKK ERRDVESFWKLNHSPGSDRHLESRDPPRLSGRDPSSWTVEDVMQFVREADPQLGSHADLFRKHEIDGKAL LLLRSDMMMKYMGLKLGPALKLSFHIDRLKQVFWKRETILWSREGLSREVWPISEDTALGHFFSGMDKVF GSLSKR TRTRPLE - GFP Tag - V
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	BC034667
ORF Size	2118 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC034667, AAH34667
RefSeq Size	2596 bp
RefSeq ORF	2120 bp
Locus ID	29871
Gene Summary	Associates with Polycomb group (PcG) multiprotein complexes; the complex class is required to maintain the transcriptionally repressive state of some genes.[UniProtKB/Swiss-Prot Function]

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