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Prmt7 (NM_145404) Mouse Tagged ORF Clone
CAT＃: MG210073
- TrueORF®
Prmt7 (tGFP-tagged) - Mouse protein arginine N-methyltransferase 7 (Prmt7)
ORF Plasmid: DDK
"NM_145404" in other vectors (4)
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CNY 6,370.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | 4933402B05Rik; BC006705 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG210073 representing NM_145404
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAAGGTCTTCTGTGGCCGTGCCAATCCTACCACGGGATCCCTGGAGTGGCTGGAGGAGGATGAACACT ATGATTACCACCAGGAGATTGCCAGGTCATCCTATGCCGACATGCTACATGACAAAGACAGAAATATAAA ATACTACCAGGGTATCCGGGCAGCTGTGAGCAGGGTGAAAGACAGAGGACAGAAGGCCTTGGTTCTTGAC ATTGGCACTGGCACAGGCCTCTTGTCAATGATGGCAGTTACTGCAGGGGCTGACTTCTGCTATGCTATCG AGGTTTTTAAGCCTATGGCTGAGGCTGCTGTGAAGATTGTGGAGAGGAATGGCTTCAGTGATAAGATTAA AGTCATTAACAAGCACTCCACTGAGGTGACAGTCGGACCAGATGGTGACTTGCCGTGTCGTGCTAACATT CTGATCACGGAGCTGTTTGACACAGAGCTGATTGGGGAGGGAGCGCTGCCCTCTTATGAGCATGCACACA AGCATCTTGTCCAGGAAGACTGCGAGGCAGTGCCACACAGGGCAACTGTCTATGCCCAGCTGGTGGAGTC CCGAAGGATGTGGTCCTGGAACAAGCTGTTTCCCGTCCGTGTCCGGACGAGTCTAGGCGAGCAGGTCATC GTCCCCCCCTCAGAATTGGAGAGGTGTCCTGGTGCGCCTTCAGTCTGTGACATTCAGCTGAACCAGGTGT CGCCTGCTGACTTCACTGTCCTCAGTGATGTGCTGCCAATGTTCAGCGTGGACTTCAGCAAGCAAGTCAG CAGCTCGGCAGCGTGCCATAGCAGGCAGTTTGTACCTTTGGCGTCTGGCCAAGCACAGGTGGTTCTGTCC TGGTGGGACATTGAAATGGACCCTGAGGGCAAGATCAAGTGCACCATGGCACCCTTTTGGGCACAGACAG ATCCGCAGGAGCTTCAGTGGCGGGACCACTGGATGCAGTGTGTGTACTTCCTGCCGCAGGAGGAGCCTGT TGTGCAGGGCTCACCCCGGTGCCTGGTAGCCCACCATGATGACTACTGTGTGTGGTACAGCCTTCAGAGA ACCAGCCCTGATGAGAACGACAGCGCCTACCAAGTGCGACCTGTGTGTGACTGTCAGGCTCACTTGCTCT GGAACCGGCCTCGGTTTGGAGAAATCAATGATCAGGACAGAACTGATCACTATGCCCAGGCCCTGAGGAC TGTGCTGCTGCCAGGTAGCGTCTGCCTTTGTGTGAGTGATGGCAGTCTCCTCTCCATGCTGGCCCATCAC CTCGGAGCGGAGCAGGTGTTTACAGTTGAGAGTTCAGTAGCTTCCTATAGACTGATGAAAAGGATCTTCA AGGTTAACCACTTGGAAGATAAAATCAGTGTCATCAATAAACGGCCTGAGTTGCTGACAGCTGCAGACCT GGAGGGCAAGAAGGTCTCCCTCCTCCTGGGTGAACCCTTTTTCACCACCAGCCTGCTGCCATGGCACAAC CTGTACTTCTGGTATGTCCGTACCTCTGTGGACCAGCACCTAGCACCTGGAGCTGTGGTGATGCCTCAGG CTGCCTCACTGCATGCCGTGATTGTGGAGTTCAGGGACCTGTGGCGGATCCGGAGTCCTTGCGGTGACTG CGAAGGTTTTGATGTGCACATCATGGATGATATGATCAAGCACTCCCTGGATTTCCGAGAGAGCAGAGAG GCAGAGCCACACCCACTGTGGGAATACCCCTGCAGAAGCCTCTCCAAGCCTCAAGAGATCCTGACTTTTG ATTTCCAGCAGCCCATCCCCCAACAGCCTATGCAATCCAAGGGCACAATGGAGCTGACAAGACCCGGGAA GAGCCATGGGGCTGTCCTGTGGATGGAGTATCAGCTCACTCCAGACAGCACGATCAGCACTGGCCTCATA AACCCTGCAGAAGACAAGGGGGACTGCTGCTGGAACCCCCACTGCAAGCAAGCTGTGTACTTCCTCAGCA CCACGCTGGATCTCAGAGTGCCTCTGAATGGCCCTCGGTCAGTCAGCTATGTTGTGGAGTTTCACCCCCT CACTGGAGACATCACCATGGAGTTTAGGCTTGCAGACACCTTGAGC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG210073 representing NM_145404
Red=Cloning site Green=Tags(s) MKVFCGRANPTTGSLEWLEEDEHYDYHQEIARSSYADMLHDKDRNIKYYQGIRAAVSRVKDRGQKALVLD IGTGTGLLSMMAVTAGADFCYAIEVFKPMAEAAVKIVERNGFSDKIKVINKHSTEVTVGPDGDLPCRANI LITELFDTELIGEGALPSYEHAHKHLVQEDCEAVPHRATVYAQLVESRRMWSWNKLFPVRVRTSLGEQVI VPPSELERCPGAPSVCDIQLNQVSPADFTVLSDVLPMFSVDFSKQVSSSAACHSRQFVPLASGQAQVVLS WWDIEMDPEGKIKCTMAPFWAQTDPQELQWRDHWMQCVYFLPQEEPVVQGSPRCLVAHHDDYCVWYSLQR TSPDENDSAYQVRPVCDCQAHLLWNRPRFGEINDQDRTDHYAQALRTVLLPGSVCLCVSDGSLLSMLAHH LGAEQVFTVESSVASYRLMKRIFKVNHLEDKISVINKRPELLTAADLEGKKVSLLLGEPFFTTSLLPWHN LYFWYVRTSVDQHLAPGAVVMPQAASLHAVIVEFRDLWRIRSPCGDCEGFDVHIMDDMIKHSLDFRESRE AEPHPLWEYPCRSLSKPQEILTFDFQQPIPQQPMQSKGTMELTRPGKSHGAVLWMEYQLTPDSTISTGLI NPAEDKGDCCWNPHCKQAVYFLSTTLDLRVPLNGPRSVSYVVEFHPLTGDITMEFRLADTLS TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_145404 |
| ORF Size | 2076 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_145404.1, NP_663379.1 |
| RefSeq Size | 2269 bp |
| RefSeq ORF | 2079 bp |
| Locus ID | 214572 |
| UniProt ID | Q922X9 |
| Gene Summary | Arginine methyltransferase that can both catalyze the formation of omega-N monomethylarginine (MMA) and symmetrical dimethylarginine (sDMA), with a preference for the formation of MMA. Specifically mediates the symmetrical dimethylation of arginine residues in the small nuclear ribonucleoproteins Sm D1 (SNRPD1) and Sm D3 (SNRPD3); such methylation being required for the assembly and biogenesis of snRNP core particles. Specifically mediates the symmetric dimethylation of histone H4 'Arg-3' to form H4R3me2s. Plays a role in gene imprinting by being recruited by CTCFL at the H19 imprinted control region (ICR) and methylating histone H4 to form H4R3me2s, possibly leading to recruit DNA methyltransferases at these sites. May also play a role in embryonic stem cell (ESC) pluripotency. Also able to mediate the arginine methylation of histone H2A and myelin basic protein (MBP) in vitro; the relevance of such results is however unclear in vivo (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC200516 | Prmt7 (untagged) - Mouse protein arginine N-methyltransferase 7 (Prmt7), (10ug) |
CNY 2,000.00 |
|
| MR210073 | Prmt7 (Myc-DDK-tagged) - Mouse protein arginine N-methyltransferase 7 (Prmt7) |
CNY 5,080.00 |
|
| MR210073L3 | Lenti ORF clone of Prmt7 (Myc-DDK-tagged) - Mouse protein arginine N-methyltransferase 7 (Prmt7) |
CNY 7,700.00 |
|
| MR210073L4 | Lenti ORF clone of Prmt7 (mGFP-tagged) - Mouse protein arginine N-methyltransferase 7 (Prmt7) |
CNY 7,700.00 |
