Lrrc49 (BC016574) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	TurboGFP
Synonyms	AI430817; AW050057; D430025H09Rik; p79; PGs4
Vector	pCMV6-AC-GFP
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MG210039 representing BC016574 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGATTCCCGGAAAATATCGCTCCGTTTCTGGCCGAGCTGCAAATAACGTGAACTGTGGACTTCATCTGG TTATTCAAACGTCGTCACTTTCTGACAAAAACAAAGTTGAATTCAGGCTTAACAGAGAAACACCACCATT TCCTGGTAGACTGTTACAGCATGACCTGGAGAGGAACTACTCAAGCAGGCAAGGTGACCACATTAGTCTG GTGAGCTCATCCATGCCGGCGTTTCCTATTCTCCAGCGCTCTTCTGAAGAGAAAACACTTTACTCAGACA GGCTGACCCTAGAAAGACAAAAGTTGACTGTGTGCCCTATCATCGACGGGGAAGAGCACCTTCGTTTGTT GAACTTTCAGCACAATTTTATAACTCGGATCCAAAATATTTCTAATCTACAGAGGCTAATATTCTTGGAC TTATATGATAACCAGATTGAAGAAATTAGTGGACTTTCTACTCTGAAATCCCTTCGTGTCCTCCTGTTAG GGAAAAACAGAATCAAGAAAATCTCAAATCTGGAGAATCTAAAAAATTTAGATGTCTTGGACCTTCATGG AAATCAGATTACCAAAATTGAAAATGTTAATCATTTGTGTGATTTGAGAGTTCTAAACCTTGCCAGGAAT CTCTTGAGTCATGTTGACAATCTCAACGGCCTGGATTCACTAACGGAACTTAACCTTCGGCACAACCAGA TCACCTTTGTGAGAGATGTGGATAATTTGCCCTGCCTCCAACGCCTCTTCCTCAGCTTCAACAATATAAC TAGCTTTGAAAGTGTTTCCTGCCTTGCCGAGTCAACTTCCCTCTCGGACATCACCTTTGATGGCAACCCA ATAGCTCAAGAGTCATGGTACAAACACACGGTCCTTCAGAATATGATGCAGCTGCGCCAGCTAGACATGA AGAGAATCACGGAAGAAGAAAGACGCGTGGCATCTGTTGTGCCCAAAAAAGAGGAGGAGAAGAAGAGGGA GAGTCACAAGCAGTCCTTGCTGAAGGAGAAGAAAAGGTTAACCATTAACAATGTCGCTCGAAAGTGGGAC TTGCAGCAGCGAGTAGCCAACATTGCCAGCACTCAGGACAGAAAGGACTCAGAGTCTCCGCCTCAGGAAT CCTGTCAGCTCGACGGAGGCAACATCTCTGCCTTTCCAGAGGAAGCAGGGTCTCTAGATGCAGGGCTCAG CAGTGCGTTACAAGGCTTGTCTGTCACAGAAACACACTTGGTGGAGATAGACGGAGAAACCCTCTCACTG TACGGCTCAGGGGCGCTGGAATGCCTGGATCGAAACTGGAGTGTTCAAACAGCCGGCATGGTCACCACGG TCTCATTCACGTTCATCGAGTTTGATGAAATTGTCCAAGTACTTCCCAAACTGAAGATGAAGTTTCCAAA TTCTCTGCACCTTAAATTCAGGGAGACCAACCTTGTGATGCTGCAGCAGTTCAATGCCCTGGCACAGCTG CGTCGGGTTGACCAGTTGACAATCGATCCTCAGGGAAACCCAGTGGTCAACTTTACACTCTGGAAGTACT ACGTGCTGTTCAGGCTCAGCCACTTCAGCATGCAGAAAATAAATGGAACAGAGGTGACTCAAAATGACAT GATAATGGCTGAAAGGCTCTTTGGAATCTTAGCACATGTAGCATCCTCCGAGCTGCCCCAGTACCGCATG ATTTCAATTCTGGGTGATGCCAGGAAAAAGCAATTCCGATATCTACTGGAAAGCAAAGGTAAAAAGCCTG GCATCATCTGTGAGGAGACCAGTGACAGCAAGAGGTTCTTGGGGGAAAACACGAACCGCGCTACCCTGAA TTACACCACCCGAGAATTCTACCACGAGAAGCTGGAAGAAATAAAGGACAAGAAGAAGTTCTGCAGGTTG TATGTAGAAGACCTGGTGAAGGAAGCCACAGCCATCAACATGAAAAACGAGGCTCTGCAGAAGCTGTGGC CACAGATGTTCATCGAGCTTGTGAGAGACGCTGTCATAGAGATCCGCAGCAAGGATTCCTACATGAAACT CTGCCTGCAGCAGATCACTGACCAGAAA AGCGGACCGACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA Protein Sequence (showhide) >MG210039 representing BC016574 Red=Cloning site Green=Tags(s) MIPGKYRSVSGRAANNVNCGLHLVIQTSSLSDKNKVEFRLNRETPPFPGRLLQHDLERNYSSRQGDHISL VSSSMPAFPILQRSSEEKTLYSDRLTLERQKLTVCPIIDGEEHLRLLNFQHNFITRIQNISNLQRLIFLD LYDNQIEEISGLSTLKSLRVLLLGKNRIKKISNLENLKNLDVLDLHGNQITKIENVNHLCDLRVLNLARN LLSHVDNLNGLDSLTELNLRHNQITFVRDVDNLPCLQRLFLSFNNITSFESVSCLAESTSLSDITFDGNP IAQESWYKHTVLQNMMQLRQLDMKRITEEERRVASVVPKKEEEKKRESHKQSLLKEKKRLTINNVARKWD LQQRVANIASTQDRKDSESPPQESCQLDGGNISAFPEEAGSLDAGLSSALQGLSVTETHLVEIDGETLSL YGSGALECLDRNWSVQTAGMVTTVSFTFIEFDEIVQVLPKLKMKFPNSLHLKFRETNLVMLQQFNALAQL RRVDQLTIDPQGNPVVNFTLWKYYVLFRLSHFSMQKINGTEVTQNDMIMAERLFGILAHVASSELPQYRM ISILGDARKKQFRYLLESKGKKPGIICEETSDSKRFLGENTNRATLNYTTREFYHEKLEEIKDKKKFCRL YVEDLVKEATAINMKNEALQKLWPQMFIELVRDAVIEIRSKDSYMKLCLQQITDQK SGPTRTRRLE - GFP Tag - V
Restriction Sites	SgfI-RsrII      Cloning Scheme for this gene
ACCN	BC016574
ORF Size	2058 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC016574, AAH16574
RefSeq Size	2566 bp
RefSeq ORF	2060 bp
Locus ID	102747

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