Zfp12 (BC053080) Mouse Tagged ORF Clone

CAT＃: MG210037

TrueORF^®

Zfp12 (tGFP-tagged) - Mouse zinc finger protein 12 (cDNA clone MGC:62453 IMAGE:5702966)

ORF Plasmid: DDK tGFP

Datasheet

SDS

Plasmid Map

"BC053080" in other vectors (4)

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Mouse Protein / Lysate Request

CNY 7510.00

货期*

4周

规格

Product images

推荐一起购买 (4)

PS100010

pCMV6-AC-GFP, mammalian vector with C-terminal tGFP tag, 10ug

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TA150041

Mouse monoclonal turboGFP antibody, clone OTI2H8

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DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)

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PRIMER

Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each

CNY 480.00

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	TurboGFP
Synonyms	C530015C18; Krox-7; Zfp-12; Znf12
Vector	pCMV6-AC-GFP
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MG210037 representing BC053080 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAATACATCCTTAGGCCCACTGTCATTCAAGGATGTGGCTGTGGCCTTCTCCCAGGAGGAGTGGCAGC AGCTGGACCCTGAGGAAAGGACGACATACAGGGATGTGATGCTGGAGACCTACAGCAACCTCGTCTCCGT GGGGTATGACATTATCAAACCGGATGTTATCATCAAGTTGGAACAAGGAGAAGAGCCATGGATAGTAGAA GGAGCGTTCTCGCCTCAGAGCTATCCAGATGAGATCCGGCACATGAGCAGGCTGATGGAGGAGGACCAGG GAGGTGAAGAGAACCAGTCAAGTTCAGCTGTGTTCAGCTACAGAAGCCGGGCTGATGCCTCTAGTAAGGC CACTGATGGAGAAACAAAGCCCTTTCCCTCACAGAAGGCCCTCCCTCAGTGCAACTCCTGTGAGAAGAGC CTGATGTGTGTCTCGGCGTTCATCCGAAGCGACGGAAGCTACGCAAAACTGAGGCCCAATGTGTGTGCCG GGTGTGGGAAGCCCCTCCCCTGCAGCAAGCCCGAGGAAACACACCCTGGAGGTGAGTCTTACGAATTTAG TGGGGATGGAGATGAGGACCCTCTCGGTGAAGAAGGCGTGTATCAGAAGGGTCACTTTTTGGAGGAGCCC TTTGAATACGTTGAGTGCCAGAAATCCTTCCCCAAGGGCACTGTGTTTCTTAATCACCTGGAAGAGGAGC CCTGCGACTGGAATGACGCTGAGGTCGCTTTCCTGCAGACGTCAGACCTCAGTGCCCACCAGGATTCTCT CATGGAAATGAAGCCCTACGAGTGCCAGCAGTGTGGGAAGTCCTTCTGCAAAAAGTCCAAGTTCGTCATC CATCAGAGGACTCACACAGGAGAGAAGCCCTTCAAGTGCAGTCAGTGTGGGAAGTCCTTCTGCCAGAAGG GCACCCTCACCGTCCACCAGAGGACGCACACCGGGGAGAAGCCCTACGAGTGTACCGAGTGCGGGAAGAC CTTCTACCAGAAGCTGCACCTCATTCAGCACCAGAGGACCCACTCGGGCGAGAAGCCCTACAAGTGTGGC TACTGCGGGAAGTCCTTCTGCCAGAAGACGCATCTCACTCAGCACCAGAGGACGCACTCGGGAGAGAGGC CCTATGTTTGTCACGACTGTGGCAAGACGTTCTCTCAGAAGTCAGCACTCAATGACCACCAGAAGATCCA CACGGGCGTCAAACTCTACAAATGCAGCGAGTGTGGCAAGTGCTTCTGCCGCAAGTCCACGCTCACAACC CACATGAGGACGCACACGGGCGAGAAGCCGTACGAATGCAACGAGTGCGGCAAGTTCTTCTCCAGGCTGT CGTACCTCACCGTGCATTACAGAACCCACTCGGGGGAGAAGCCCTACGAGTGTGCGGAGTGCGGGAAGTC CTTCTACCTGAACTCAGCCCTTATGAGACATCAGAGGGTGCACACGGGGGAGAAGCCTTACGAGTGTAAC GAGTGTGGGAAATTATTCTCGCAGCTGTCCTACCTCACCGTGCATCACAGAACTCACTCAGGAGTGAAGC CCTATGAGTGTAGCGAGTGTGGAAAGACCTTCTACCAGAATTCAGCCCTCTGTAGACACCGGAGAATTCA CAGAGGGGAGAAGCCCTATGAATGCTACATCTGTGGCAAGTTCTTTTCTCAGATGTCTTACCTCACGATC CATCACAGAATTCACTCAGGGGAGAAGCCCTATGAGTGCCGCGAGTGTGGGAAGTCCTTCTGCCAGAACT CAGCCCTCAACAGACACCAGCGCACGCACACGGGAGAAAAAGCCTACGAGTGTTACGAGTGTGGGAAATG CTTCTCCCAGATGTCTTATCTCACTATCCATCACAGGATCCACTCGGGAGAGAAGCCCTTCGAGTGTAAC GAGTGTGGGAAAGCCTTCTCTCGGATGTCGTACCTCACTGTGCACCACAGGACCCACTCAGGAGAGAAGC CCTATGAATGTACGGAGTGCGGGAAGAAATTCTACCACAAGTCCGCGTTCAATAGCCATCAGAGAACTCA CAGGAGAGGGAGCGGGAATGGGGTTGAT ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA Protein Sequence (showhide) >MG210037 representing BC053080 Red=Cloning site Green=Tags(s) MNTSLGPLSFKDVAVAFSQEEWQQLDPEERTTYRDVMLETYSNLVSVGYDIIKPDVIIKLEQGEEPWIVE GAFSPQSYPDEIRHMSRLMEEDQGGEENQSSSAVFSYRSRADASSKATDGETKPFPSQKALPQCNSCEKS LMCVSAFIRSDGSYAKLRPNVCAGCGKPLPCSKPEETHPGGESYEFSGDGDEDPLGEEGVYQKGHFLEEP FEYVECQKSFPKGTVFLNHLEEEPCDWNDAEVAFLQTSDLSAHQDSLMEMKPYECQQCGKSFCKKSKFVI HQRTHTGEKPFKCSQCGKSFCQKGTLTVHQRTHTGEKPYECTECGKTFYQKLHLIQHQRTHSGEKPYKCG YCGKSFCQKTHLTQHQRTHSGERPYVCHDCGKTFSQKSALNDHQKIHTGVKLYKCSECGKCFCRKSTLTT HMRTHTGEKPYECNECGKFFSRLSYLTVHYRTHSGEKPYECAECGKSFYLNSALMRHQRVHTGEKPYECN ECGKLFSQLSYLTVHHRTHSGVKPYECSECGKTFYQNSALCRHRRIHRGEKPYECYICGKFFSQMSYLTI HHRIHSGEKPYECRECGKSFCQNSALNRHQRTHTGEKAYECYECGKCFSQMSYLTIHHRIHSGEKPFECN ECGKAFSRMSYLTVHHRTHSGEKPYECTECGKKFYHKSAFNSHQRTHRRGSGNGVD TRTRPLE - GFP Tag - V
Restriction Sites	SgfI-MluI Cloning Scheme for this gene Plasmid Map
ACCN	BC053080
ORF Size	2058 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC053080.1
RefSeq Size	4315 bp
RefSeq ORF	2060 bp
Locus ID	231866
Gene Summary	Transcriptional repressor which suppresses activation protein 1 (AP-1)- and serum response element (SRE)-mediated transcriptional activity.[UniProtKB/Swiss-Prot Function]

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Documents

Product Manuals
TrueORFs: Open Reading Frame Clones - 10 ug in PrecisionShuttle Vector System pLenti-ORF destination vector Transfection - TurboFectin 8.0 Transfection Reagent Plasmid Preparation - PowerPrep Plasmid Purification Kits
FAQs
TrueORF Clones/PrecisionShuttle Organelle Marker Transfection Reagent
SDS
TrueORF SDS

Resources

cDNA 克隆相关资源
Video Tutorial: Clone Selection Guide Product Video: What is TrueORF GOLD and why it is the most popular type of clone Video Tutorial: How to make Lentivirus particles Video Tutorial: Is the Lentivirus Safe Product Brochure: cDNA clones $98 Clones. Download the List of 15,000 Protocol: Lentivirus packaging

Other Versions

SKU	Description	Size	Price
MC220912	Zfp12 (untagged) - Mouse zinc finger protein 12 (cDNA clone MGC:62453 IMAGE:5702966), (10ug)	10 ug 产品选项验证失败所有选项都是必需的	CNY 9120.00
MR210037	Zfp12 (Myc-DDK-tagged) - Mouse zinc finger protein 12 (cDNA clone MGC:62453 IMAGE:5702966)	10 ug 产品选项验证失败所有选项都是必需的	CNY 6840.00
MR210037L3	Lenti ORF clone of Zfp12 (Myc-DDK-tagged) - Mouse zinc finger protein 12 (cDNA clone MGC:62453 IMAGE:5702966)	10 ug 产品选项验证失败所有选项都是必需的	CNY 8740.00
MR210037L4	Lenti ORF clone of Zfp12 (mGFP-tagged) - Mouse zinc finger protein 12 (cDNA clone MGC:62453 IMAGE:5702966)	10 ug 产品选项验证失败所有选项都是必需的	CNY 8740.00

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