Klhl22 (BC005800) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	TurboGFP
Synonyms	2610318I18Rik; Kelchl
Vector	pCMV6-AC-GFP
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MG209634 representing BC005800 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCTGAGGAGCAGGACTTTGCCCAGCTGTGCAGGCTGCCCACACAGCCCTCCCACTCCCACTGTGTCA ACAATACCTATCGAAGCACACAGCACTCCCAGGCCCTGCTCCGGGGCCTGCTGGCTTTGCGAGACAGTGG GATCCTCTTTGACGTTGTCCTGGTGGTAGAGGGGAAGCACATTGAGGCCCATCGGATTCTGCTAGCTGCA TCGTGTGATTATTTCAGAGGAATGTTTGCTGGGGGATTGAAGGAGATGGAGCAGGAAGAGGTCTTGATCC ACGGTGTGTCTTACAATGCCATGTGCCAAATCCTGCACTTCATATACACTTCTGAACTAGAGCTCAGCCT GAGCAATGTGCAGGAGACCCTGGTTGCTGCCTGCCAACTTCAGATACCAGAAATTATCCACTTCTGCTGT GATTTCCTCATGTCATGGGTAGATGAGGAGAATATCCTTGATGTCTACCGGCTAGCAGACCTCTTTGACT TGAACCATCTGACCCAACAGCTAGATACCTACATCCTCAAAAACTTTGTGGCCTTCTCCAGAACTGACAA GTACCGCCAGCTTCCCTTGGAGAAGGTCTACTCTCTCCTCAGCAGCAATCGCCTGGAGGTTTCCTGTGAG ACTGAAGTGTATGAAGGGGCCCTGCTCTACCATTACTCCCTAGAGCAGGTGCAGGCTGACCAGATCTCAC TGAATGAGCCCCCCAAGCTCCTAGAGACTGTGCGCTTTCCCCTGATGGAAGCTGAGGTCCTTCAGCGGCT GCATGACAAGCTTGGTCCTAGCCCACTGAGGGACACAGTGGCCAGTGCCCTTATGTACCACCGGAATGAG ATCCTGCAGCCCAGCCTTCAGGGCCCACAGACAGAGCTTCGCTCGGACTTCCAGTGTGTTGTGGGCTTTG GGGGTATTCACTCCACACCGTCTACAATCCTCAGTGACCAGGCCAAGTACCTGAATCCTCTACTGGGAGA GTGGAAACACTTCACTGCCTCTCTGGCGCCCCGAATGTCCAATCAGGGCATTGCAGTGCTCAACAACTTC GTGTACTTAATTGGAGGGGACAATAATGTCCAAGGATTTCGAGCTGAGTCCCGATGCTGGAGGTATGACC CAAGGCACAATCGCTGGTTCCAGATCCAGTCCTTGCAGCAAGAACATGCAGACCTATGTGTGTGTGTAGT GGGCAAGTACATCTATGCTGTGGCAGGCCGTGACTACCACAATGACCTGAGTGCTGTGGAGCGCTATGAC CCTGCCACCAACTCCTGGGACTATGTGGCCCCACTCAAGAAGGAGGTATATGCCCATGCAGGCACCACAC TGCAGGGGAAGATGTATATTACCTGTGGTCGCAGAGGAGAGGACTACCTGAAAGAGACACACTGCTATGA CCCAGGAAGCAACACGTGGCATACTCTGGCTGACGGGCCTGTGCGACGGGCCTGGCATGGCATGGCTGCC CTCCTCGACAAGCTGTTTGTGATTGGAGGCAGCAACAATGATGCTGGCTACAGAAGGGATGTGCACCAGG TGGCTTGCTACAGTTGTACATCTCGTCAGTGGTCATCGGTCTGCCCACTCCCAGCTGGGCATGGTGAGCC TGGCATTGCTGTGCTAGACAGCAGGATATATGTGCTGGGTGGCCGCTCTCACAACCGAGGCAGCCGTACA GGCTACGTGCACATCTACGACATGGAGAAAGACTGCTGGGAAGAGGGACCCCAGCTGAACAATTCCATCT CAGGCCTGGCAGCCTGTGTGCTCACCCTGCCTCGATCCCTGCTCCATGAGCAACCCCGAGGGACCCCCAA CCGTAGCCAGGCAGATGCAGACTTTGCTTCTGAGGTGATGAGTGTGTCTGACTGGGAAGAGTTTGACAAC TCTAGCGAGGAC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA Protein Sequence (showhide) >MG209634 representing BC005800 Red=Cloning site Green=Tags(s) MAEEQDFAQLCRLPTQPSHSHCVNNTYRSTQHSQALLRGLLALRDSGILFDVVLVVEGKHIEAHRILLAA SCDYFRGMFAGGLKEMEQEEVLIHGVSYNAMCQILHFIYTSELELSLSNVQETLVAACQLQIPEIIHFCC DFLMSWVDEENILDVYRLADLFDLNHLTQQLDTYILKNFVAFSRTDKYRQLPLEKVYSLLSSNRLEVSCE TEVYEGALLYHYSLEQVQADQISLNEPPKLLETVRFPLMEAEVLQRLHDKLGPSPLRDTVASALMYHRNE ILQPSLQGPQTELRSDFQCVVGFGGIHSTPSTILSDQAKYLNPLLGEWKHFTASLAPRMSNQGIAVLNNF VYLIGGDNNVQGFRAESRCWRYDPRHNRWFQIQSLQQEHADLCVCVVGKYIYAVAGRDYHNDLSAVERYD PATNSWDYVAPLKKEVYAHAGTTLQGKMYITCGRRGEDYLKETHCYDPGSNTWHTLADGPVRRAWHGMAA LLDKLFVIGGSNNDAGYRRDVHQVACYSCTSRQWSSVCPLPAGHGEPGIAVLDSRIYVLGGRSHNRGSRT GYVHIYDMEKDCWEEGPQLNNSISGLAACVLTLPRSLLHEQPRGTPNRSQADADFASEVMSVSDWEEFDN SSED TRTRPLE - GFP Tag - V
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene
ACCN	BC005800
ORF Size	1902 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC005800, AAH05800
RefSeq Size	2610 bp
RefSeq ORF	1904 bp
Locus ID	224023
Gene Summary	Substrate-specific adapter of a BCR (BTB-CUL3-RBX1) E3 ubiquitin ligase complex required for chromosome alignment and localization of PLK1 at kinetochores. The BCR(KLHL22) ubiquitin ligase complex mediates monoubiquitination of PLK1, leading to PLK1 dissociation from phosphoreceptor proteins and subsequent removal from kinetochores, allowing silencing of the spindle assembly checkpoint (SAC) and chromosome segregation. Monoubiquitination of PLK1 does not lead to PLK1 degradation (By similarity). The BCR(KLHL22) ubiquitin ligase complex is also responsible for the amino acid-stimulated 'Lys-48' polyubiquitination and proteasomal degradation of DEPDC5. Through the degradation of DEPDC5, releases the GATOR1 complex-mediated inhibition of the TORC1 pathway. It is therefore an amino acid-dependent activator within the amino acid-sensing branch of the TORC1 pathway, indirectly regulating different cellular processes including cell growth and autophagy (PubMed:29769719).[UniProtKB/Swiss-Prot Function]

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