Cc2d1a (BC016188) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	TurboGFP
Synonyms	MGC38983, MGC27560
Vector	pCMV6-AC-GFP
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MG209260 representing BC016188 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGCGGTACCATGTGGCTGCAGCCCAGGCCAAGGCCAAGGGTGACCAGCGGAAGGCACGCATGCATG AACGAATTGTCAAGCAATACCAAGACGCCATTCGTGCCCACAAGGCAGGCCGAGCTGTGGATGTGGCGGA GCTGCCAGTGCCCCCAGGCTTCCCCCCAATGCAGGGTTTGGAGTCTGCAGAGCCCTCTCAACAGAGCCTA GTGGGTGTCCTGGAAACTGCCATGAGGCTTGCCAACCACGACGAAGGCTCGGATGACGAAGAGGAGGAGA CTCCTAAGAAGCAGAATACCCCTGCTGCCTCCACAACCCAGCTGAAGTCCTCGCCCTCCAAAGCACCTCC ATCAGGACCAGCCCCAGCCGGCAAAGCTGCCCCCAAGGGCACATCCAACAGAGCCCAGCAGCAGCTGGCC TTCCTGGAGGGCCGTAAGAAGCAGCTCCTGCAGGCCGCGCTGCGTGCCAAGCAAAAGAACGACGTAGAAG GCGCCAAGATGCACCTGCGCCAGGCCAAAGGGCTAGAGCCCATGCTAGAGGCCTCTCGCAATGGACTGCC TGTGGACATTGCCAAGGTGCCACCTGCTCCTGTCAACAAGGACGACTTTGTGCTGGTGCAGCGGCCTGGC CCAGGCTTGTCTCAGGAAGCTGTGCGTCGCTATGGGGAACTTACCAAGCTCCTGAGACAGCAACATGAAA TGTGTCTAAACCACTCCACACAGTTCACCCACCTGGGCAACATTGCTGAAACCATTAAGTTCGAGAAGCT GGCAGAAGACTGTAAGCGGAGCATGGACACCTTAAAACAAGCCTTCGCCCGCAGTCTGCCCACACCCGCA GCCCGCTTTGAGCAGAGGACCTTCAGTGTCATCAAGGTCTTTCCGGACTTGAGCAACAGTGACATGCTCC TGTTTATCGTGAAGGGCATCAACTTGCCCACACCCACAGGGCTGTCCCCCAGTGACCTGGATGCCTTTGT CCGCTTTGACTTCCCTTATCCCAATGTGGAAGAAGCTCAGAAAGACAAGACCAGTGTGATCAAAAACACA GACTCCCCTGAATTTAAGGAGCAGTTCAAACTCTGCATCAACCGTGGCCACCGTGGCTTCCGAAGGGCCA TCCAAACCAAGGGCATCAAGTTCGAAGTGGTCCACAAGGGGGGGCTGTTCAAGACGGACAGGGTGCTAGG TACAGCCCAGCTGAAGCTGGGTACGTTGGAAACAGCTTGTGAAGTCCACGAGATCCTGGAGGTTTTGGAT GGACGCCGACCCACGGGGGGGAGGCTTGAAGTGATGGTTCGCATCCGGGAGCCACTGACAGCCCAGCAGT TGGAAACTACAACTGAGAGGTGGCTGGTCATTGATCACATCCCAGCAGCTATGCCCACTGTCACTGGGCC CAAAGCAAAGGCTCCTCTCATACCGGCGTCTTCAAGGGAAGCAGGCAACAGATCAGCACGGCCCCTGCAT AGCCTTAGCGTGTTGGCCTTTGACCAAGAGCGGCTAGAAAGGAAGATCCTGGCCCTGAGGCAGGCACGCC GGCCTGTGCCTCCAGAAGTGGCCCAGCAGTACCAGGACGTAGTACAGCGTAGCCAATGGCAGAGGGCACA GCTGGAGCAGGGGGGTGCTGCCCTCCGCAGGGAGTATGCAAGCCATCTGGAGCGCCAGCTGCACTTCTAT ACAGAGGCTGCCCGGCGCCTGGGCTACGATGGAAGCAGGGAGGCTGCAAAGGAGGCATTGTATCGGCGGA ACCTGGTGGAGAGCGAGCTGCAGCGACTCCGCAGG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA Protein Sequence (showhide) >MG209260 representing BC016188 Red=Cloning site Green=Tags(s) MERYHVAAAQAKAKGDQRKARMHERIVKQYQDAIRAHKAGRAVDVAELPVPPGFPPMQGLESAEPSQQSL VGVLETAMRLANHDEGSDDEEEETPKKQNTPAASTTQLKSSPSKAPPSGPAPAGKAAPKGTSNRAQQQLA FLEGRKKQLLQAALRAKQKNDVEGAKMHLRQAKGLEPMLEASRNGLPVDIAKVPPAPVNKDDFVLVQRPG PGLSQEAVRRYGELTKLLRQQHEMCLNHSTQFTHLGNIAETIKFEKLAEDCKRSMDTLKQAFARSLPTPA ARFEQRTFSVIKVFPDLSNSDMLLFIVKGINLPTPTGLSPSDLDAFVRFDFPYPNVEEAQKDKTSVIKNT DSPEFKEQFKLCINRGHRGFRRAIQTKGIKFEVVHKGGLFKTDRVLGTAQLKLGTLETACEVHEILEVLD GRRPTGGRLEVMVRIREPLTAQQLETTTERWLVIDHIPAAMPTVTGPKAKAPLIPASSREAGNRSARPLH SLSVLAFDQERLERKILALRQARRPVPPEVAQQYQDVVQRSQWQRAQLEQGGAALRREYASHLERQLHFY TEAARRLGYDGSREAAKEALYRRNLVESELQRLRR TRTRPLE - GFP Tag - V
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene
ACCN	BC016188
ORF Size	1785 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC016188, AAH16188
RefSeq Size	3349 bp
RefSeq ORF	1787 bp
Locus ID	212139
Gene Summary	Transcription factor that binds specifically to the DRE (dual repressor element) and represses HTR1A gene transcription in neuronal cells. The combination of calcium and ATP specifically inactivates the binding with FRE. May play a role in the altered regulation of HTR1A associated with anxiety and major depression. Mediates HDAC-independent repression of HTR1A promoter in neuronal cell. Performs essential function in controlling functional maturation of synapses.[UniProtKB/Swiss-Prot Function]

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.