5430413K10Rik (BC080814) Mouse Tagged ORF Clone
CAT＃: MG209220
- TrueORF®
5430413K10Rik (tGFP-tagged) - Mouse RIKEN cDNA 5430413K10 gene (cDNA clone MGC:91146 IMAGE:6391119)
ORF Plasmid: DDK
"BC080814" in other vectors (4)
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CNY 3710.00
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Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | 4833413D08Rik; AI413838 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG209220 representing BC080814
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTGGGTTCTCCAGGCCTTGGCCATCATGTTGAGCATCCAAGCGGGAACACTTGACCTGGTGGAAACAC CCCCAGTGGTGGGCAATCTTCCTGTTGCCATGCCTGTTCCACTCAATCTCCCTGTAGGAGGCCTAAGTCC TCCAGTCCTCAAAGGACCAGTCAACCATCAAATGTTACCTCCTAAAAGGCCTGTACCTCCACCCAAAGGT GGCAAGTGTGCACCTGCAGCCAGATACTTCCTCTCCAGTGACAAACTCCATGACTACCTCATGAGCACAC TGCCCCCACAGATTGAGGACATGGTGAAGTGTGATGAAGTTAACCTGGAAGGCATGCTTGCGGATGTGTT GAACACTGTGGAGAGCTCTGACCTGCTCTCCCTCTTAGATGGCATTTCCCTCCTAAAAGGAGGAGAAGGG GGAGGCCTTGGCATTGGTGGACTCCTAGGCAATGAAGGCAATGGGGATTCCTCAAAGCCCTCATCAGGCT CCAAGGCCACTGGTGGTCTTGGCCAGCTAATCCCAGGGGGCATCCCAGGCACGGAGGCCCTGGGCGGCCT ACTAAACCTCGGTGGAGACAAGAGCTCTGGAAAAGGGCTGCTGAATGGGGATGGGCTCTCCAAAATCAAG AAGCCTCTTGAGGATGCAGTTGAAAATGTGAGCGGTATAAAAGATGCCATTCAGGAGAAAGTGAATGAAG TGGTCCCAGATGGCGTCAAGGAGCCACTTAACGATGTGCTCAAGATGGATATCAAAGACACTCTGCTCGA ACTGAAGGTCGGACAAGTAACTTTGGACGACATGGAGATAAACATGGAAGCTAATGGCATGCAAGTTCTC TCCATGCTTACTGCTACCATAGATGGAAAAGGCGTGCTTGGACCAGTCATCAGCCTACTGCAGTTTGAAG CTAAGATGGATGTAATGACGACAATTGCTGTTGCCTCCAACAACACCCAGTGTGTCAACCTTGATGCCCA AGACACCCACATGCATGTCAAGGAAATGAAAATTCAGTTAGTGGAGACAGTCACAGGAAAAGTGCCTCTG CCTGTGCCTCTGCCCTTGGATCAGATCATCCCAGCAATAGTGACAGCAAAAATAAATGAAAATCTGGAAA AATCCAACTCCTGTGCCATTGTCCTCAATGACTTCAACAACTGCAAGAACAATACCGGCTTATTCAGTTA CCAAGTTAATACTGCCAGGATCTCTCCAAAAGGACTTGTCATCCTCTACTGTGCTAAAGCCAATATAGGC AACAAAACAGTACCTGTGCCTGGAGGTCGGCTGCCTCCAGATCCCAAAAATGCAAGCATTGCGGTAACCA TTTCCTCCACAACACTGAAGACACTTGTAAAAGAAGTGGCCAAGAATAGCTCTGTGCAGATGGATGGCCT GGAAGCACAAATCACCCACATAGCGTTTGCCTCCCAGGAAAACAATACGCTCAGAGTCGTGTATAAGGTT GACATAACAAAGAACGGAGAGCACTTTGCCACTGGGGAAACGAAATTATTCATCTCCCATGGCAGCAAGA TTTCAAACTCCACACTGATACCAGATGTCAAGCTCATAAGGTCTGAGCACAGTGTGGTGCCTCCTGAGGC TAAGGAAGAAGTAGAAGGCATTTTGTCTGAAGTAGGAAAGGTGGCCTGGTCCAACTTCAATGAAACGTAT AAAAAAATGAACATTCCTGTAGGTGTGTCTTCACATACTTTAAAGAACTCCGATGTCAAATTGATGAAAT CAATTGACCTTCAGGCAGCAAGC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG209220 representing BC080814
Red=Cloning site Green=Tags(s) MWVLQALAIMLSIQAGTLDLVETPPVVGNLPVAMPVPLNLPVGGLSPPVLKGPVNHQMLPPKRPVPPPKG GKCAPAARYFLSSDKLHDYLMSTLPPQIEDMVKCDEVNLEGMLADVLNTVESSDLLSLLDGISLLKGGEG GGLGIGGLLGNEGNGDSSKPSSGSKATGGLGQLIPGGIPGTEALGGLLNLGGDKSSGKGLLNGDGLSKIK KPLEDAVENVSGIKDAIQEKVNEVVPDGVKEPLNDVLKMDIKDTLLELKVGQVTLDDMEINMEANGMQVL SMLTATIDGKGVLGPVISLLQFEAKMDVMTTIAVASNNTQCVNLDAQDTHMHVKEMKIQLVETVTGKVPL PVPLPLDQIIPAIVTAKINENLEKSNSCAIVLNDFNNCKNNTGLFSYQVNTARISPKGLVILYCAKANIG NKTVPVPGGRLPPDPKNASIAVTISSTTLKTLVKEVAKNSSVQMDGLEAQITHIAFASQENNTLRVVYKV DITKNGEHFATGETKLFISHGSKISNSTLIPDVKLIRSEHSVVPPEAKEEVEGILSEVGKVAWSNFNETY KKMNIPVGVSSHTLKNSDVKLMKSIDLQAAS TRTRPLE - GFP Tag - V |
| Restriction Sites | SgfI-MluI Cloning Scheme for this gene |
| ACCN | BC080814 |
| ORF Size | 1773 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | BC080814, AAH80814 |
| RefSeq Size | 2070 bp |
| RefSeq ORF | 1775 bp |
| Locus ID | 71425 |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC217875 | 5430413K10Rik (untagged) - Mouse RIKEN cDNA 5430413K10 gene (cDNA clone MGC:91146 IMAGE:6391119), (10ug) |
CNY 6940.00 |
|
| MR209220 | 5430413K10Rik (Myc-DDK-tagged) - Mouse RIKEN cDNA 5430413K10 gene (cDNA clone MGC:91146 IMAGE:6391119) |
CNY 4400.00 |
|
| MR209220L3 | Lenti ORF clone of 5430413K10Rik (Myc-DDK-tagged) - Mouse RIKEN cDNA 5430413K10 gene (cDNA clone MGC:91146 IMAGE:6391119) |
CNY 5230.00 |
|
| MR209220L4 | Lenti ORF clone of 5430413K10Rik (mGFP-tagged) - Mouse RIKEN cDNA 5430413K10 gene (cDNA clone MGC:91146 IMAGE:6391119) |
CNY 5230.00 |
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