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Edem2 (NM_145537) Mouse Tagged ORF Clone
CAT＃: MG209050
- TrueORF®
Edem2 (tGFP-tagged) - Mouse ER degradation enhancer, mannosidase alpha-like 2 (Edem2)
ORF Plasmid: DDK
"NM_145537" in other vectors (4)
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CNY 5,510.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | 9530090G24Rik; AI327354 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG209050 representing NM_145537
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCTTTCCGGCTACTCATCCCGCTCGGCCTCGTGTGCGTGCTGCTACCCCTGCACCATGGTGCGCCAG GCCCCGACGGCACCGCGCCCGACCCCGCCCACTACAGGGAGCGAGTCAAGGCCATGTTCTACCACGCCTA CGACAGTTACCTGGAAAATGCCTTTCCCTACGATGAGCTGAGACCTCTCACCTGTGATGGGCACGACACC TGGGGCAGTTTTTCTCTGACGCTAATTGATGCCCTGGACACCTTGCTGATTTTGGGGAATACCTCTGAAT TCCAAAGAGTAGTGGAGGTTCTTCAGGACAATGTGGACTTTGATATCGACGTGAACGCCTCTGTGTTCGA AACCAACATCCGAGTGGTAGGAGGACTCCTTTCTGCTCATCTCTTGTCAAAGAAGGCCGGTGTGGAGGTG GAAGCCGGGTGGCCTTGTTCTGGGCCTCTCCTCAGGATGGCGGAGGAAGCAGCCCGGAAACTCCTCCCAG CATTTCAGACCCCCACGGGCATGCCCTATGGAACTGTGAACCTGCTTCATGGCGTGAACCCTGGAGAGAC CCCTGTTACCTGTACAGCAGGGATTGGGACCTTCATTGTGGAATTTGCTACCCTGAGCAGCCTCACCGGT GACCCTGTGTTTGAAGATGTGGCCAGAGTGGCCCTGATGCGTCTCTGGGAGAGCCGGTCAGATATCGGGC TGGTTGGCAATCACATCGATGTGCTCACTGGCAAGTGGGTGGCCCAGGATGCAGGCATCGGGGCTGGTGT GGATTCCTATTTTGAGTACCTGGTAAAAGGAGCCATCCTTCTTCAGGATAAGAAACTCATGGCCATGTTC CTAGAGTACAACAAAGCCATCCGGAATTACACGCACTTCGATGACTGGTACCTCTGGGTGCAGATGTACA AGGGGACCGTGTCAATGCCTGTCTTCCAGTCCTTGGAGGCCTACTGGCCTGGCCTTCAGAGCCTTATTGG AGACATTGACAATGCCATGAGGACCTTTCTCAACTACTACACTGTATGGAAGCAGTTTGGGGGGCTGCCA GAATTCTACAACATTCCTCAGGGATACACAGTGGAGAAGCGAGAAGGCTACCCACTCCGACCGGAGCTCA TCGAGAGCGCAATGTACCTCTACCGCGCCACTGGGGATCCCACTCTCTTAGAACTTGGGAGAGACGCTGT GGAATCCATTGAGAAAATCAGCAAGGTGGAGTGTGGATTTGCCACGATCAAGGACCTCCGGGACCACAAG CTTGACAACCGCATGGAGTCTTTCTTCCTGGCTGAAACTGTGAAATACCTCTATCTTCTGTTCCACCCAA ACAACTTCATCCACAACAATGGCTCCACCTTTGACTCTGTGATGACTCCCCATGGGGAGTGCATCCTAGG AGCCGGGGGCTACATCTTCAACACGGAGGCTCACCCCATTGACCCTGCTGCCTTGCACTGCTGCCGGAGG CTCAAGGAGGAGCAGTGGGAAGTGGAGGACTTGATAAAGGAGTTCTACTCCCTCAAACAGAGCAGGCCAA AGAGAGCTCAGAGGAAGACAGTGAGGTCGGGGCCCTGGGAGCCCCAGTCAGGGCCAGCCACACTCTTCTC ACCTGCAAACCAGCCCAGGGAGAAGCAGCCTGCCCGGCAGCGAACCCCGCTTCTCAGCTGCCCCAGCCAG CCCTTCACCTCTAAGCTGGCATTGCTAGGCCAGGTTTTCCTAGACTCCTCA ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG209050 representing NM_145537
Red=Cloning site Green=Tags(s) MPFRLLIPLGLVCVLLPLHHGAPGPDGTAPDPAHYRERVKAMFYHAYDSYLENAFPYDELRPLTCDGHDT WGSFSLTLIDALDTLLILGNTSEFQRVVEVLQDNVDFDIDVNASVFETNIRVVGGLLSAHLLSKKAGVEV EAGWPCSGPLLRMAEEAARKLLPAFQTPTGMPYGTVNLLHGVNPGETPVTCTAGIGTFIVEFATLSSLTG DPVFEDVARVALMRLWESRSDIGLVGNHIDVLTGKWVAQDAGIGAGVDSYFEYLVKGAILLQDKKLMAMF LEYNKAIRNYTHFDDWYLWVQMYKGTVSMPVFQSLEAYWPGLQSLIGDIDNAMRTFLNYYTVWKQFGGLP EFYNIPQGYTVEKREGYPLRPELIESAMYLYRATGDPTLLELGRDAVESIEKISKVECGFATIKDLRDHK LDNRMESFFLAETVKYLYLLFHPNNFIHNNGSTFDSVMTPHGECILGAGGYIFNTEAHPIDPAALHCCRR LKEEQWEVEDLIKEFYSLKQSRPKRAQRKTVRSGPWEPQSGPATLFSPANQPREKQPARQRTPLLSCPSQ PFTSKLALLGQVFLDSS TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_145537 |
| ORF Size | 1731 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_145537.1, NP_663512.1 |
| RefSeq Size | 1926 bp |
| RefSeq ORF | 1734 bp |
| Locus ID | 108687 |
| UniProt ID | Q8BJT9 |
| Gene Summary | Involved in the endoplasmic reticulum-associated degradation (ERAD) pathway that targets misfolded glycoproteins for degradation in an N-glycan-dependent manner (PubMed:15579471, PubMed:25655076). May initiate ERAD by promoting the first mannose trimming step of ERAD substrates, from Man9GlcNAc2 to Man8GlcNAc2 (By similarity). Seems to recognize and bind to exposed hydrophobic regions in target proteins (PubMed:25655076).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC200261 | Edem2 (untagged) - Mouse ER degradation enhancer, mannosidase alpha-like 2 (Edem2), (10ug) |
CNY 2,000.00 |
|
| MR209050 | Edem2 (Myc-DDK-tagged) - Mouse ER degradation enhancer, mannosidase alpha-like 2 (Edem2) |
CNY 4,304.00 |
|
| MR209050L3 | Lenti ORF clone of Edem2 (Myc-DDK-tagged) - Mouse ER degradation enhancer, mannosidase alpha-like 2 (Edem2) |
CNY 6,940.00 |
|
| MR209050L4 | Lenti ORF clone of Edem2 (mGFP-tagged) - Mouse ER degradation enhancer, mannosidase alpha-like 2 (Edem2) |
CNY 6,940.00 |
