Gpc6 (BC023448) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	TurboGFP
Vector	pCMV6-AC-GFP
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MG208930 representing BC023448 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCTTCTTGGATCCGGGCTGTGATTCTTCCTCTCTCCGGGCTGCTGCTGACCCTCCCGGCCGCGGCGG ACGTGAAGGCTCGGAGCTGCAGCGAGGTCCGTCAGGCTTACGGTGCCAAGGGATTCAGCCTGGCGGACAT CCCCTACCAGGAGATCGCAGGGGAGCACTTACGCATCTGCCCTCAGGAATATACCTGCTGCACCACAGAA ATGGAAGACAAGCTGAGTCAACAGAGTAAACTGGAGTTTGAAAACCTTGTAGAAGAGACAAGCCACTTTG TGAGGACCACGTTTGTGTCGAGGCACAAGAAATTTGATGAGTTTTTCCGAGAGCTGCTGGAAAACGCAGA AAAGTCCCTAAATGACATGTTTGTCCGGACCTACGGGATGCTGTACATGCAGAATTCAGAGGTATTCCAG GACCTCTTCACTGAGCTAAAGCGGTACTACACAGGGGGTAACGTCAACCTGGAAGAGATGCTCAATGACT TCTGGGCTCGGCTCCTGGAGAGGATGTTCCAGCTGATTAACCCTCAGTATCACTTCAGCGAGGACTACCT GGAGTGTGTAAGCAAGTACACAGACCAGCTGAAGCCATTTGGAGACGTGCCACGGAAACTGAAGATTCAG GTCACCCGCGCCTTCATCGCGGCTCGCACCTTTGTCCAGGGCCTGACAGTGGGCAGAGAGGTTGCCAACC GAGTTTCCAAGGTGAGCCCCACCCCTGGCTGCATCCGGGCTCTCATGAAGATGCTGTACTGCCCATACTG CCGAGGCTTGCCCACTGTGAGACCTTGCAACAACTACTGCCTCAACGTCATGAAGGGCTGCCTGGCCAAT CAGGCAGATTTGGACACTGAGTGGAATCTCTTCATAGATGCAATGCTCTTGGTGGCTGAGCGACTGGAAG GGCCATTCAACATTGAGTCAGTCATGGACCCAATAGACGTCAAGATCTCTGAAGCCATTATGAACATGCA GGAAAACAGCATGCAGGTGTCAGCAAAGGTCTTTCAGGGCTGTGGCCAGCCCAAACCTGCTCCCGCTCTC AGATCTGCTCGCTCAGCTCCAGAAAATTTTAACACCCGTTTTCGGCCCTACAACCCTGAGGAAAGACCCA CAACTGCAGCGGGCACGAGCTTGGACCGGCTGAGGACTGTGTGGAGGACCATGCAGCACAGTGTCACAGA CATAAAAGAGAAGCTGAAGCTCTCTAAAAAGGTCTGGTCAGCATTGCCCTACACCATCTGCAAGGACGAA CGTGTGACAGCAGGAACCTCCAACGAGGAGGAGTGCTGGAACGGACACAGCAAAGCCAGATACCTGCCTG AGATCATGAACGATGGGCTGACCAACCAAATCAACAATCCTGAGGTGGAGGTGGACATCACACGGCCAGA CACTTTCATCAGACAGCAGATCATGGCTCTCCGTGTGATGACCAACAAACTGAAGAATGCGTACAATGGC AATGACGTCAACTTCCAAGACACAAGCGATGAATCCAGTGGCTCAGGCAGCGGCAGTGGTTGCATGGATG ATGTGTGTCCCACGGAGTTCGAGTTTGTCACCACCGAGGCCCCGGCGGTGGACCCGGACCGCAGAGAAGA GGAATCTTCGGCCTCGAAGTTCAGCTCCTCCTTGATTTCCTGGTCTCTGGTCTGCATGGTCTTGGCCCTG CAGAGACTCTACAGA ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA Protein Sequence (showhide) >MG208930 representing BC023448 Red=Cloning site Green=Tags(s) MPSWIRAVILPLSGLLLTLPAAADVKARSCSEVRQAYGAKGFSLADIPYQEIAGEHLRICPQEYTCCTTE MEDKLSQQSKLEFENLVEETSHFVRTTFVSRHKKFDEFFRELLENAEKSLNDMFVRTYGMLYMQNSEVFQ DLFTELKRYYTGGNVNLEEMLNDFWARLLERMFQLINPQYHFSEDYLECVSKYTDQLKPFGDVPRKLKIQ VTRAFIAARTFVQGLTVGREVANRVSKVSPTPGCIRALMKMLYCPYCRGLPTVRPCNNYCLNVMKGCLAN QADLDTEWNLFIDAMLLVAERLEGPFNIESVMDPIDVKISEAIMNMQENSMQVSAKVFQGCGQPKPAPAL RSARSAPENFNTRFRPYNPEERPTTAAGTSLDRLRTVWRTMQHSVTDIKEKLKLSKKVWSALPYTICKDE RVTAGTSNEEECWNGHSKARYLPEIMNDGLTNQINNPEVEVDITRPDTFIRQQIMALRVMTNKLKNAYNG NDVNFQDTSDESSGSGSGSGCMDDVCPTEFEFVTTEAPAVDPDRREEESSASKFSSSLISWSLVCMVLAL QRLYR TRTRPLE - GFP Tag - V
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene
ACCN	BC023448
ORF Size	1695 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC023448, AAH23448
RefSeq Size	2100 bp
RefSeq ORF	1697 bp
Locus ID	23888
Gene Summary	Cell surface proteoglycan that bears heparan sulfate. Putative cell surface coreceptor for growth factors, extracellular matrix proteins, proteases and anti-proteases. Enhances migration and invasion of cancer cells through WNT5A signaling (By similarity).[UniProtKB/Swiss-Prot Function]

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