Tbc1d17 (BC017607) Mouse Tagged ORF Clone
CAT＃: MG208694
- TrueORF®
Tbc1d17 (tGFP-tagged) - Mouse TBC1 domain family, member 17 (cDNA clone MGC:27642 IMAGE:4507589)
ORF Plasmid: DDK
"BC017607" in other vectors (4)
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CNY 5990.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | MGC27642 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG208694 representing BC017607
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAAGGATCCAGCTACAGGGTGGTGTTTGAAAAGGGAGGCGTGTACCTGCACACCAGCGCCCGGAAAC ACCAGGATCCAGACTCGCTCATCGCCGGCGTCATACGAGTGGTGGAAAAGGACAGCGACGTCTTTCTGCA CTGGGCTCCGGTGGAGGAGGCTGGAGACCCAACCCAGATTCTCTTCAAGAAGGACCCGAGTCGGGGTGAG CCCAGCACCTCAGAGGAGGAACCTACCTTTGACCCAGGCTATGAACCTGACTGGGCTGTCATTAGCACGG TGCGGCCACAGCCCCACCTGGCAGAGCCCAGGAAAGGTGCAGAGCCCAGTTCCTCCCGGAGCTCCTGGGC CTTCTCCGTGAGCCTGGGGGAGCTCAAGTCCATCCGCAGATCCAAGCCAGGCCTCAGCTGGGCCTACCTG GTCCTGGTGACCCAGGCTGGAGGCTCCCTGCCTGCCTTACACTTTCACAGAGGGGGTACCCGAGCCCTGC TCCGAGTCCTTAGCCGATACCTGCTGTTGGCCAGCTCTCCACAGGACTCCCGCCTCTACTTGGTCTTCCC CCAGGACCCCTCTGCCCTGTCCGACTCCTTCCACCACCTGCAGCTCTTTGACCAGGACAGCTCCAATGTG GTGTCTCGTTTCCTTCAGGACCCCTACTCCACCACCTTCAGTAGCTTCTCTCGAGTGACCAACTTCTTCC GGGGAGCCCTACAGCCGCACCCTGAGGGCGCCTCCTCCCCTAACCTACCCCCGCTGCCAGATGATGAACC AGAGCCTGGCTTTGAGGTCATTTCTTGCGTGGAGCTGGGGCAGCGTCCCACAGTGGAGCGTGCTCCTCCA GTCACAGAGGAAGAGTGGAACCGCTACGTGGGCCCCGAGGGTCGCCTGCAGAATGTCCCTGAGTTGAAAA ACCGGATCTTCTCAGGCGGTCTCAGCCCTGGCCTACGTCGAGAGGCTTGGAAGTTCCTTCTGGGGTACCT TAGCTGGGAGAGCTCAGCTGAAGAGCACAAGGCGCATGTGCGGAAGAAAACGGATGAGTACTTCCGAATG AAGCTGCAGTGGAAGTCTGTGAGTGCTGAGCAGGAGCGGAGGAACTCACTGCTGCATGGATACCGAAGCC TCATTGAGAGAGATGTAAGCCGCACGGACAGGACTAACAAGTTCTATGAAGGCCCTGAGAACCCTGGGCT GAGCCTGCTCCATGATATCCTTCTTACCTACTGCATGTACCACTTCGACTTGGGCTACGTTCAGGGTATG AGTGACCTCCTGTCCCCGATCCTCTTCGTTGTTCAGAACGAAGTAGATGCCTTCTGGTGTTTCTGCGGCT TCATGGAACTTGTGCATGGGAACTTTGAAGAGAGTCAGGAGACTATGAAGCGGCAGCTTGGGCAGCTCCT GCTGCTTCTGCGGGTGCTGGATCAGCCACTCTGTGATTTCCTGGATTCTCAGGACTCGGGCTCGCTGTGC TTCTGTTTCCGGTGGCTGCTCATCTGGTTCAAGAGGGAATTCCCCTTTCCTGATGTCCTCCGGCTGTGGG AGGTGGGCGGCCAGTTGGGGTGCTGTGGACAGGGCTTCCTGGCCCCAATTTGCACCTGCTGGTGGCCTGT GCCATCTTGGATATGGAACGGGACACCC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG208694 representing BC017607
Red=Cloning site Green=Tags(s) MEGSSYRVVFEKGGVYLHTSARKHQDPDSLIAGVIRVVEKDSDVFLHWAPVEEAGDPTQILFKKDPSRGE PSTSEEEPTFDPGYEPDWAVISTVRPQPHLAEPRKGAEPSSSRSSWAFSVSLGELKSIRRSKPGLSWAYL VLVTQAGGSLPALHFHRGGTRALLRVLSRYLLLASSPQDSRLYLVFPQDPSALSDSFHHLQLFDQDSSNV VSRFLQDPYSTTFSSFSRVTNFFRGALQPHPEGASSPNLPPLPDDEPEPGFEVISCVELGQRPTVERAPP VTEEEWNRYVGPEGRLQNVPELKNRIFSGGLSPGLRREAWKFLLGYLSWESSAEEHKAHVRKKTDEYFRM KLQWKSVSAEQERRNSLLHGYRSLIERDVSRTDRTNKFYEGPENPGLSLLHDILLTYCMYHFDLGYVQGM SDLLSPILFVVQNEVDAFWCFCGFMELVHGNFEESQETMKRQLGQLLLLLRVLDQPLCDFLDSQDSGSLC FCFRWLLIWFKREFPFPDVLRLWEVGGQLGCCGQGFLAPICTCWWPVPSWIWNGTP TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | BC017607 |
| ORF Size | 1638 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | BC017607.1 |
| RefSeq Size | 2376 bp |
| RefSeq ORF | 1640 bp |
| Locus ID | 233204 |
| Gene Summary | Probable GTPase-activating protein for Rab8; its transient association with Rab8 is mediated by OPTN. Inhibits Rab8-mediated endocytic trafficking, such as of transferrin receptor (TfR) and reduces Rab8 recruitnment to tubules emanating from the endocytic recycling compartment (ERC). Involved in regulation of autophagy. Mediates inhibition of autophagy caused by the OPTN variant GLC1E LYS-50; the function requires its catalytic activity, however, the involved Rab is not known (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC218617 | Tbc1d17 (untagged) - Mouse TBC1 domain family, member 17 (cDNA clone MGC:27642 IMAGE:4507589), (10ug) |
CNY 6940.00 |
|
| MR208694 | Tbc1d17 (Myc-DDK-tagged) - Mouse TBC1 domain family, member 17 (cDNA clone MGC:27642 IMAGE:4507589) |
CNY 5420.00 |
|
| MR208694L3 | Lenti ORF clone of Tbc1d17 (Myc-DDK-tagged) - Mouse TBC1 domain family, member 17 (cDNA clone MGC:27642 IMAGE:4507589) |
CNY 7320.00 |
|
| MR208694L4 | Lenti ORF clone of Tbc1d17 (mGFP-tagged) - Mouse TBC1 domain family, member 17 (cDNA clone MGC:27642 IMAGE:4507589) |
CNY 7320.00 |
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