Nrf1 (BC005410) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	TurboGFP
Synonyms	C87038; D6Ertd415e
Vector	pCMV6-AC-GFP
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MG208550 representing BC005410 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGGAGCACGGAGTGACCCAAACTGAACACATGGCTACCATAGAAGCCCATGCAGTGGCCCAGCAAG TCCAACAGGTCCATGTAGCCACGTACACTGAGCACAGTATGCTAAGTGCTGATGAAGACTCCCCTTCCTC CCCCGAGGACACTTCTTATGATGACTCGGACATCCTCAACTCCACGGCAGCTGATGAGGTAACTGCCCAT CTGGCTGCTGCAGGTCCTGTGGGAATGGCCGCTGCTGCTGCTGTGGCAACAGGGAAGAAACGGAAACGGC CTCATGTGTTTGAGTCTAATCCATCTATCCGAAAGAGACAGCAGACACGTTTGCTTCGGAAACTCAGAGC CACGTTGGATGAGTACACGACGCGAGTGGGACAGCAAGCGATTGTACTCTGCATCTCACCCTCCAAACCC AACCCTGTCTTCAAGGTGTTTGGCGCAGCACCTTTGGAGAATGTGGTGCGAAAGTACAAGAGCATGATCC TGGAAGACCTCGAGTCTGCTCTGGCAGAACACGCCCCTGCGCCACAGGAGGTTAATTCAGAGCTGCCGCC TCTCACCATCGATGGGATTCCAGTCTCTGTGGACAAAATGACCCAGGCTCAGCTTCGGGCATTTATCCCA GAGATGCTCAAGTATTCCACAGGTCGGGGGAAACCAGGCTGGGGGAAAGAAAGCTGCAAGCCTATCTGGT GGCCAGAAGATATCCCATGGGCCAATGTCCGCAGTGATGTCCGCACAGAAGAGCAAAAACAAAGGGTTTC ATGGACCCAGGCATTACGGACCATAGTTAAAAATTGCTATAAGCAACATGGGCGGGAGGATCTTTTATAT GCTTTTGAAGATCAGCAAACACAAACTCAGGCCACCACCACACACAGTATAGCTCATCTCGTACCATCAC AGACCGTAGTACAGACCTTCAGCAACCCTGATGGCACCGTGTCGCTCATCCAGGTTGGTACAGGGGCAAC AGTAGCCACATTGGCTGATGCTTCAGAACTGCCAACCACAGTCACTGTTGCCCAAGTGAATTACTCTGCT GTGGCTGATGGAGAGGTGGAACAAAATTGGGCCACGTTACAGGGCGGTGAAATGACCATCCAGACGACGC AAGCATCAGAGGCCACCCAGGCGGTAGCATCACTGGCAGAAGCCGCAGTGGCAGCTTCTCAGGAGATGCA ACAGGGAGCCACTGTCACCATGGCCCTCAACAGTGAAGCTGCCGCCCATGCTGTCGCCACTCTGGCGGAA GCCACCTTACAAGGTGGGGGACAGATAGTCCTGTCTGGGGAAACCGCAGCAGCCGTCGGAGCACTTACTG GAGTCCAAGATGCTAATGGCCCAGATGGAGTTCATGCATGGACCATCAGCAAAGCCGTGACGGCTCCTCA GGCTGCAGGAAAGATTCATACAGATTTTGGAAAGGGATTCATTATGGCGGAAGTAATGAAAGACGAAGAT CTTAAAGAGGAAAGTTCTGAGAACGCAGCTAAGGCTGCTGGGAAGTACAAACAACAAGGCAGAGCTTACG TTGTTGAAGACGGAGATATAATCTTCTTCAAATTTAATTCGCCTCAGCAATTAAAAAAGAAA ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA Protein Sequence (showhide) >MG208550 representing BC005410 Red=Cloning site Green=Tags(s) MEEHGVTQTEHMATIEAHAVAQQVQQVHVATYTEHSMLSADEDSPSSPEDTSYDDSDILNSTAADEVTAH LAAAGPVGMAAAAAVATGKKRKRPHVFESNPSIRKRQQTRLLRKLRATLDEYTTRVGQQAIVLCISPSKP NPVFKVFGAAPLENVVRKYKSMILEDLESALAEHAPAPQEVNSELPPLTIDGIPVSVDKMTQAQLRAFIP EMLKYSTGRGKPGWGKESCKPIWWPEDIPWANVRSDVRTEEQKQRVSWTQALRTIVKNCYKQHGREDLLY AFEDQQTQTQATTTHSIAHLVPSQTVVQTFSNPDGTVSLIQVGTGATVATLADASELPTTVTVAQVNYSA VADGEVEQNWATLQGGEMTIQTTQASEATQAVASLAEAAVAASQEMQQGATVTMALNSEAAAHAVATLAE ATLQGGGQIVLSGETAAAVGALTGVQDANGPDGVHAWTISKAVTAPQAAGKIHTDFGKGFIMAEVMKDED LKEESSENAAKAAGKYKQQGRAYVVEDGDIIFFKFNSPQQLKKK TRTRPLE - GFP Tag - V
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	BC005410
ORF Size	1604 bp
OTI Disclaimer	Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	BC005410, AAH05410
RefSeq Size	1767 bp
RefSeq ORF	1604 bp
Locus ID	18181
Gene Summary	Transcription factor that activates the expression of the EIF2S1 (EIF2-alpha) gene. Links the transcriptional modulation of key metabolic genes to cellular growth and development. Implicated in the control of nuclear genes required for respiration, heme biosynthesis, and mitochondrial DNA transcription and replication (By similarity).[UniProtKB/Swiss-Prot Function]

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