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Nup62 (NM_053074) Mouse Tagged ORF Clone
CAT＃: MG208443
- TrueORF®
Nup62 (tGFP-tagged) - Mouse nucleoporin 62 (Nup62)
ORF Plasmid: DDK
"NM_053074" in other vectors (4)
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CNY 5,800.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | D7Ertd649e; Nupc1; p62 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG208443 representing NM_053074
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGTGGATTTAACTTTGGAGGCACCGGGGCTCCTGCTGGCGGCTTTACATTTGGGACCGCAAAGACTG CGACCACCACACCTGCCACTGGCTTTTCCTTCTCTGCTTCTGGCACCGGCACCGGAGGGTTTAATTTTGG GACTCCCTCCCAGCCAGCTGCGACCACCCCTTCCACCAGCCTCTTCTCACTCACCACACAGACCCCAACC ACACAGACCCCAGGATTCAACTTTGGAACAACACCTGCTTCTGGAGGAACAGGCTTCTCCCTGGGGATCA GCACCCCAAAGCTCAGCCTGAGTAACGCAGCTGCCACACCAGCCACGGCCAACACTGGCAGCTTTGGGCT TGGCAGCAGCACCCTCACCAATGCCATCTCAAGTGGCAGCACCTCCAACCAGGGGACAGCCCCCACTGGC TTTGTCTTTGGCTCTTCTACCACCTCTGCTCCGTCCACCGGCTCCACTGGGTTCTCATTCACCAGTGGCA GTGCATCCCAGCCTGGAGCCTCTGGCTTCAGCCTTGGCTCTGTGGGTAGCTCTGCCCAGCCCACAGCACT GTCTGGCTCTCCCTTCACACCAGCCACTCTGGTGACTACTACAGCAGGAGCTACACAGCCAGCTGCTGCT GCACCCACTGCTGCCACCACCAGTGCAGGGTCTACACTCTTTGCTTCCATAGCTGCTGCTCCTGCCTCAT CCAGTGCTACAGGGCTCTCCCTCCCAGCTCCGGTGACAACTGCAGCAACTCCTAGTGCTGGGACTTTGGG CTTCAGCCTCAAGGCCCCTGGAGCAGCTCCTGGTGCCTCCACCACCAGCACCACCACTACCACTACCACC ACTACTACCACTGCTGCTGCCGCTGCCGCCTCTACCACAACCACTGGCTTTGCCTTAAGTCTGAAACCCC TGGTGTCAGCTGGCCCCAGCAGTGTAGCAGCTACTGCTCTGCCTGCCTCCAGCACAGCAGCTGGGACTGC CACAGGTCCAGCGATGACCTATGCACAGCTGGAAAGCTTGATCAACAAGTGGAGTCTGGAGCTGGAAGAT CAGGAGCGGCACTTCCTGCAGCAGGCCACACAGGTCAATGCCTGGGACCGCACACTGATTGAGAATGGGG AGAAGATCACCAGCCTGCACCGAGAGGTGGAAAAGGTGAAGCTGGATCAGAAGCGACTGGACCAGGAGCT GGACTTTATCCTGTCGCAGCAGAAGGAGCTGGAAGACCTCCTGAGCCCATTGGAGGAGTCAGTGAAGGAG CAAAGTGGCACCATATACCTTCAGCACGCTGATGAGGAGCGTGAGAAGACCTACAAGCTGGCTGAGAACA TTGATGCTCAGCTCAAGCGCATGGCACAGGACCTCAAGGATATCATCGAGCACCTGAACATGGCTGGTGG CCCTGCAGACACCAGTGACCCATTGCAACAGATCTGCAAGATCCTCAATGCACACATGGACTCTCTTCAG TGGGTGGACCAGAGCTCTGCCCTGCTGCAGAGGAGGGTGGAGGAGGCTAGCCGTGTATGTGAGGGCCGGC GCAAGGAACAGGAGCGCAGTCTGCGCATTGCCTTTGAC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG208443 representing NM_053074
Red=Cloning site Green=Tags(s) MSGFNFGGTGAPAGGFTFGTAKTATTTPATGFSFSASGTGTGGFNFGTPSQPAATTPSTSLFSLTTQTPT TQTPGFNFGTTPASGGTGFSLGISTPKLSLSNAAATPATANTGSFGLGSSTLTNAISSGSTSNQGTAPTG FVFGSSTTSAPSTGSTGFSFTSGSASQPGASGFSLGSVGSSAQPTALSGSPFTPATLVTTTAGATQPAAA APTAATTSAGSTLFASIAAAPASSSATGLSLPAPVTTAATPSAGTLGFSLKAPGAAPGASTTSTTTTTTT TTTTAAAAAASTTTTGFALSLKPLVSAGPSSVAATALPASSTAAGTATGPAMTYAQLESLINKWSLELED QERHFLQQATQVNAWDRTLIENGEKITSLHREVEKVKLDQKRLDQELDFILSQQKELEDLLSPLEESVKE QSGTIYLQHADEEREKTYKLAENIDAQLKRMAQDLKDIIEHLNMAGGPADTSDPLQQICKILNAHMDSLQ WVDQSSALLQRRVEEASRVCEGRRKEQERSLRIAFD TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_053074 |
| ORF Size | 1578 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_053074.2, NP_444304.1 |
| RefSeq Size | 2397 bp |
| RefSeq ORF | 1581 bp |
| Locus ID | 18226 |
| UniProt ID | Q63850 |
| Gene Summary | Essential component of the nuclear pore complex. The N-terminal is probably involved in nucleocytoplasmic transport. The C-terminal is involved in protein-protein interaction probably via coiled-coil formation, promotes its association with centrosomes and may function in anchorage of p62 to the pore complex. Plays a role in mitotic cell cycle progression by regulating centrosome segregation, centriole maturation and spindle orientation. It might be involved in protein recruitment to the centrosome after nuclear breakdown.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC217430 | Nup62 (untagged) - Mouse nucleoporin 62 (Nup62), (10ug) |
CNY 6,750.00 |
|
| MR208443 | Nup62 (Myc-DDK-tagged) - Mouse nucleoporin 62 (Nup62) |
CNY 5,230.00 |
|
| MR208443L3 | Lenti ORF clone of Nup62 (Myc-DDK-tagged) - Mouse nucleoporin 62 (Nup62) |
CNY 7,130.00 |
|
| MR208443L4 | Lenti ORF clone of Nup62 (mGFP-tagged) - Mouse nucleoporin 62 (Nup62) |
CNY 7,130.00 |
