Obsl1 (BC051643) Mouse Tagged ORF Clone
CAT＃: MG208436
- TrueORF®
Obsl1 (tGFP-tagged) - Mouse obscurin-like 1 (cDNA clone MGC:59625 IMAGE:6512900)
ORF Plasmid: DDK
"BC051643" in other vectors (4)
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CNY 5800.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | AW822216 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG208436 representing BC051643
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCATGCCCTGGTCCTTCCAGGGGTGACCCGAGAAGATGCTGGAGAGATCACCTTCAGCCTGGGCAACT CCCGTACCACCACCCTGCTCAGAGTCAAATGTGTCAAGCACAGTCCTCCCGGGCCCCCCGTAATGGTTGA GATGTTCAAAGGCCAAAAGAACAAGGTTCTGCTGACCTGGAAGCCCCCAGAGCCACCTCCAGAGACCTCC TTCATCTACCGCCTAGAGCGGCAGGAAGTGGGGTCTGACGATTGGATCCAGTGCTTCAGCATTGAGAAGG CGGGAGCCGTAGAGGTGCCCGGGGACTGTGTACCCACCGAGGGTGACTATCACTTCCGCATCTGCACAGT CAGTGAACATGGCCGCAGTCCCCATGTCGTGTTCAACGGTTCTGCTCACCTTGTGCCCACAGCTCGCCTG GTGTCAGGCCTGGAAGACGTGCAGGTGTATGATGGGGAAGATGCCGTCTTCTCCCTTGATCTGTCCGCCA TCATCCAGGGCTCTTGGTTCCTTAATGGAGAGCAGCTCCAGAGTAATGAGCCAGAGGGCCAGGTGGAGCC TGGAGCCCTGCGGTACCGCATAGAGCAGAAGGGCCTCCAGCACAGGCTCATCCTGCAAGCTGTCAAGCAC CGGGACAGTGGGGCCCTGGTTGGCTTCAGCTGCCCTGGTGTGCAAGACTCTGCTGCCCTCACTATCCAAG AAAGCTCAGTGCACATCCTGAGTCCCCAGGACAAGGTGTCACTGACCTTCACGACCTCTGAACGGGTAGT GCTGACCTGTGAGCTCTCAAGGGTGGATTTTCCTGCAACCTGGTACAAGGATGGGCAGAAGGTGGAGGAG AGCGAGTCGCTCATAGTGAAGACAGAGGGCCGCAAACACCGACTGATCCTGCCTGAGGCTCAAGTCCGAG ACAGCGGTGAAATTGAGTGCAGAACGGAAGGGGTCTCGGCCTTCTTTGGAGTCACTGTTCAAGATCCCCC AGTGCACATCGTGAACCCCCAAGAGCATGTGTTTGTCCATGCCATCACCTCCGAGTGTGTCAGGCTGACC TGTGAGGTAGACCGAGAGGACACAACTGTACACTGGTACAAGGATGGGCAGGAGGTGGAGGAGAGTGACA TCATCGTATTAGAAAATGAAGGGCCCCATCACCGCCTGGTGCTACCTGCAGCCCGGCCCTCCGACGGGGG CGAGTTCCAGTGTGTCGCAGGAGATGAACGTGCCTACTTCACAGTTACCATCACAGATGTCTTCTCGTGG ATCGTCTACCCCAGTAGCGAAGTGCATGTGGCAGCCGTACGCCTAGAGCGTGTGGTGCTGACCTGTGAGC TGTGCCGACCCTGGGCTGAGGTGCGCTGGACCAAAGATGGGGAGGAGGTAGTGGAGAGCCCAGCACTGCT CCTGGAGAAGGAAGACACCATCCGCCGCCTGGTGCTGCCCTCTGTCCAGCTTGAGGATTCTGGCGAGTAC CTGTGTGAAATCCATGATGAGTCGGCTTCCTTCACCATCACCGTCACAGGTGTGGGCCTATCCCAGCCCC CAGAGTCACCAGAGGACAACCCTGAGCCTCAGGAATGT ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG208436 representing BC051643
Red=Cloning site Green=Tags(s) MHALVLPGVTREDAGEITFSLGNSRTTTLLRVKCVKHSPPGPPVMVEMFKGQKNKVLLTWKPPEPPPETS FIYRLERQEVGSDDWIQCFSIEKAGAVEVPGDCVPTEGDYHFRICTVSEHGRSPHVVFNGSAHLVPTARL VSGLEDVQVYDGEDAVFSLDLSAIIQGSWFLNGEQLQSNEPEGQVEPGALRYRIEQKGLQHRLILQAVKH RDSGALVGFSCPGVQDSAALTIQESSVHILSPQDKVSLTFTTSERVVLTCELSRVDFPATWYKDGQKVEE SESLIVKTEGRKHRLILPEAQVRDSGEIECRTEGVSAFFGVTVQDPPVHIVNPQEHVFVHAITSECVRLT CEVDREDTTVHWYKDGQEVEESDIIVLENEGPHHRLVLPAARPSDGGEFQCVAGDERAYFTVTITDVFSW IVYPSSEVHVAAVRLERVVLTCELCRPWAEVRWTKDGEEVVESPALLLEKEDTIRRLVLPSVQLEDSGEY LCEIHDESASFTITVTGVGLSQPPESPEDNPEPQEC TRTRPLE - GFP Tag - V |
| Restriction Sites | SgfI-MluI Cloning Scheme for this gene |
| ACCN | BC051643 |
| ORF Size | 1578 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | BC051643.1 |
| RefSeq Size | 2417 bp |
| RefSeq ORF | 1580 bp |
| Locus ID | 98733 |
| Gene Summary | Core component of the 3M complex, a complex required to regulate microtubule dynamics and genome integrity. It is unclear how the 3M complex regulates microtubules, it could act by controlling the level of a microtubule stabilizer. Acts as a regulator of the Cul7-RING(FBXW8) ubiquitin-protein ligase, playing a critical role in the ubiquitin ligase pathway that regulates Golgi morphogenesis and dendrite patterning in brain. Required to localize CUL7 to the Golgi apparatus in neurons (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC218514 | Obsl1 (untagged) - Mouse obscurin-like 1 (cDNA clone MGC:59625 IMAGE:6512900), (10ug) |
CNY 6940.00 |
|
| MR208436 | Obsl1 (Myc-DDK-tagged) - Mouse obscurin-like 1 (cDNA clone MGC:59625 IMAGE:6512900) |
CNY 5230.00 |
|
| MR208436L3 | Lenti ORF clone of Obsl1 (Myc-DDK-tagged) - Mouse obscurin-like 1 (cDNA clone MGC:59625 IMAGE:6512900) |
CNY 7130.00 |
|
| MR208436L4 | Lenti ORF clone of Obsl1 (mGFP-tagged) - Mouse obscurin-like 1 (cDNA clone MGC:59625 IMAGE:6512900) |
CNY 7130.00 |
