Spata2 (BC034597) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	TurboGFP
Synonyms	MGC38980, mKIAA0757
Vector	pCMV6-AC-GFP
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MG208260 representing BC034597 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGATACGAAGTACAAGGATGACTTATTCCGGAAGTACGTGCAGTTCCATGAGGGCAAAGTGGACACCA CCCCCGGCAACCAGCAGCCTGGCAGCGATGAGTACCTGCGCGTGGCAGCTGCCACCCTGCTCAGCCTGCA CAAGGTGGACCCTTTATATCGATTTCGGCTGATCCAGTTTTATGAGGTGGTGGAGAGCTCCCTCCGCTCG CTGAGCAGCTCCAGCCTGAGTGCTCTGCACTGCGCCTTCAGCATGCTGGAGACAATGGCCATCAACCTCT TCCTGTTCCCCTGGAAGAAGGAGTTCCGCAGCATCAAGACCTACACTGGCCCTTTTGTTTACTATGTCAA GTCCACGTTGCTGGAGAAGGACATCCGAGCCATTCTAAGGTTCATGGGCTACGAGCCTGAGTTGGGGACT GTGTACAAACTCAAAGAGCTTGTGGAGTCCCTCCAGGTGAAGATGGTCTCCTTTGAGCTCTTCCTGGCCA AGGTCGAGTGTGAGCAGATGCTGGGCATCCACTCGCAGGTGAAGGACAAGGGCTACTCAGAGCTGGACGT GGTGGCTGAGCGCAAGGGCAGCACGGAGGATGCGCGCGGGTGCTCAGATGCTCTGCGGAGGCGGGCTGAG AGTCGGGAGCACCTGACCACTTCCATGGCTCGTGTGGCACTCCAGAAATCAGCCAGTGAGCGAGCAGCCA AGGACTACTACAAGCCCCGGGTGACCAAACCCTCCAGGTCTGTGGATGCCTACGACAGCTACTGGGAGAG CAGGAAGCCCCCTTCAAAGGCCTCACTGAGTCTGCGCAAGGAGCCCCTGGCCATGGATGTAGGGGAAGAC TTGAAGGACGAGATCATCCGCCCATCCCCCTCATTGCTGGCCATGTCCAGCTCCCCCCATGGTAGCCCTG ATGACCTTTCCTCCATCTCCTCCATCAATGGCCTTGGCCTTCTTCGTAGTACATACTTTTCCACTCAGGA TGACGTGGACCTGTATACGGATTCAGAACCCAGGGCCACCTACCGGAGGCAGGATGCTCTGCGGCCCGAT GTGTGGCTGGTCAAAAATGATACCCACCCCATCTACCACAAGCGTTCACCCCCCACCAAAGAGTCTGCCC TCTCCAAGTGCCAAAACTGCGGCCTGTCCTGCAGCTCCTCCCTCTGCCAGCGCTGTGACAGTGTGCTGGT CTGTCCTTCGGCCTCCAAGCCCAGCGCTTTCCCCAGCAAGGCTTCTGTACACGACAGCTTGGCCCACGGG GCACCTATGCGGGAGAAGTATGTGGGCCACCAGACTCAGGGCCTTGACCGGCTGGCACCTGTCCACTCAA AGCCCAAGCCCTCTACCACAGCCACCTCCCGCTGTGGCTTCTGTAACCGTGCAGGTGCCACCAACACGTG CACCCAGTGTTCAAAAGTTTCCTGTGACGCCTGCCTCGGCGCCTACCACTACGACCCGTGCTGCAGAAAG AGTGAGCTGCACAAGTTCCTGCCCAACAGTCAGCTGAACTACAAGTCCGCCCCGTTCTCCCAGCTCGTGT ACAGA ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA Protein Sequence (showhide) >MG208260 representing BC034597 Red=Cloning site Green=Tags(s) MDTKYKDDLFRKYVQFHEGKVDTTPGNQQPGSDEYLRVAAATLLSLHKVDPLYRFRLIQFYEVVESSLRS LSSSSLSALHCAFSMLETMAINLFLFPWKKEFRSIKTYTGPFVYYVKSTLLEKDIRAILRFMGYEPELGT VYKLKELVESLQVKMVSFELFLAKVECEQMLGIHSQVKDKGYSELDVVAERKGSTEDARGCSDALRRRAE SREHLTTSMARVALQKSASERAAKDYYKPRVTKPSRSVDAYDSYWESRKPPSKASLSLRKEPLAMDVGED LKDEIIRPSPSLLAMSSSPHGSPDDLSSISSINGLGLLRSTYFSTQDDVDLYTDSEPRATYRRQDALRPD VWLVKNDTHPIYHKRSPPTKESALSKCQNCGLSCSSSLCQRCDSVLVCPSASKPSAFPSKASVHDSLAHG APMREKYVGHQTQGLDRLAPVHSKPKPSTTATSRCGFCNRAGATNTCTQCSKVSCDACLGAYHYDPCCRK SELHKFLPNSQLNYKSAPFSQLVYR TRTRPLE - GFP Tag - V
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	BC034597
ORF Size	1545 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC034597, AAH34597
RefSeq Size	3739 bp
RefSeq ORF	1547 bp
Locus ID	263876
Gene Summary	Bridging factor that mediates the recruitment of CYLD to the LUBAC complex, thereby regulating TNF-alpha-induced necroptosis (By similarity). Acts as a direct binding intermediate that bridges RNF31/HOIP, the catalytic subunit of the LUBAC complex, and the deubiquitinase (CYLD), thereby recruiting CYLD to the TNF-R1 signaling complex (TNF-RSC) (By similarity). Required to activate the 'Met-1'- (linear) and 'Lys-63'-linked deubiquitinase activities of CYLD (PubMed:28701375). Controls the kinase activity of RIPK1 and TNF-alpha-induced necroptosis by promoting 'Met-1'-linked deubiquitination of RIPK1 by CYLD (PubMed:28701375).[UniProtKB/Swiss-Prot Function]

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