Brunol4 (BC048405) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	TurboGFP
Synonyms	CELF4, Brul4, BRUNOL-4
Vector	pCMV6-AC-GFP
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MG208099 representing BC048405 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTATATAAAGATGGCCACGTTAGCAAACGGACAGGCTGACAACGCGAGCCTCAGTACCAACGGGCTAG GCAGCAGCCCGGGCAGCGCCGGGCATATGAACGGATTAAGCCACAGCCCGGGGAACCCGTCGACCATTCC CATGAAGGACCACGATGCCATCAAGCTGTTCATTGGGCAGATCCCCCGAAACCTGGATGAGAAGGACCTC AAGCCCCTCTTCGAGGAGTTCGGCAAGATCTACGAGCTTACGGTTCTGAAGGACAGGTTCACAGGCATGC ACAAAGGCTGCGCTTTCCTCACCTACTGCGAGCGTGAGTCAGCGCTGAAGGCCCAGAGCGCGCTGCACGA GCAGAAGACCCTGCCCGGGATGAACCGGCCGATCCAGGTGAAGCCTGCGGACAGCGAGAGCCGAGGAGGT AGTAGCTGCCTGCGCCAGCCCCCTTCACATAGAAAACTCTTCGTGGGTATGCTCAACAAGCAACAATCTG AGGACGACGTGCGCCGCCTCTTCGAGGCCTTCGGGAACATCGAGGAGTGCACTATCCTGCGCGGGCCGGA CGGCAACAGCAAGGGGTGCGCCTTTGTGAAGTACTCCTCCCATGCCGAGGCACAAGCCGCCATCAACGCT CTACATGGCAGCCAGACCATGCCTGGAGCCTCCTCCAGCCTGGTGGTCAAGTTTGCAGACACTGACAAGG AGCGCACAATGCGACGGATGCAGCAGATGGCTGGCCAGATGGGCATGTTCAACCCCATGGCCATCCCCTT CGGAGCCTATGGCGCCTATGCTCAGGCACTGATGCAGCAGCAAGCAGCCCTCATGGCATCGGTCGCGCAA GGAGGCTACCTGAATCCCATGGCTGCCTTCGCTGCCGCCCAAATGCAGCAGATGGCGGCCCTCAACATGA ATGGCCTGGCAGCCGCACCTATGACCCCAACCTCAGGTGGCAGCACCCCTCCAGGCATCACTGCACCAGC TGTGCCTAGCATCCCATCCCCCATTGGGGTGAACGGCTTCACGGGCCTCCCCCCTCAGGCCAATGGGCAG CCTGCTGCGGAAGCTGTGTTTGCCAATGGCATTCACCCCTACCCAGCACAGAGCCCCACCGCAGCCGACC CCCTGCAGCAGGCCTACGCTGGAGTGCAGCAGTATGCAGGACCTGCCTACCCTGCTGCCTATGGTCAGAT TAGCCAGGCCTTTCCTCAGCCACCGCCAATGATTCCCCAGCAACAGAGAGAAGGGCCCGAGGGCTGTAAC CTGCTCATCTACCATCTGCCCCAGGAGTTTGGGGACGCTGAGCTGATGCAGATGTTCCTCCCTTTCGGTA ATGTCATCTCCTCGAAAGTGTTTGTGGATCGGGCGACTAACCAAAGTAAATGCTTTGGCTTCGTGAGCTT CGACAACCCGGCCAGCGCACAGACCGCCATCCAGGCCATGAACGGCTTCCAGATAGGCATGAAGAGGCTC AAGGTGCAGCTGAAGCGGCCCAAAGACGCCAATCGCCCGTAC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA Protein Sequence (showhide) >MG208099 representing BC048405 Red=Cloning site Green=Tags(s) MYIKMATLANGQADNASLSTNGLGSSPGSAGHMNGLSHSPGNPSTIPMKDHDAIKLFIGQIPRNLDEKDL KPLFEEFGKIYELTVLKDRFTGMHKGCAFLTYCERESALKAQSALHEQKTLPGMNRPIQVKPADSESRGG SSCLRQPPSHRKLFVGMLNKQQSEDDVRRLFEAFGNIEECTILRGPDGNSKGCAFVKYSSHAEAQAAINA LHGSQTMPGASSSLVVKFADTDKERTMRRMQQMAGQMGMFNPMAIPFGAYGAYAQALMQQQAALMASVAQ GGYLNPMAAFAAAQMQQMAALNMNGLAAAPMTPTSGGSTPPGITAPAVPSIPSPIGVNGFTGLPPQANGQ PAAEAVFANGIHPYPAQSPTAADPLQQAYAGVQQYAGPAYPAAYGQISQAFPQPPPMIPQQQREGPEGCN LLIYHLPQEFGDAELMQMFLPFGNVISSKVFVDRATNQSKCFGFVSFDNPASAQTAIQAMNGFQIGMKRL KVQLKRPKDANRPY TRTRPLE - GFP Tag - V
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	BC048405
ORF Size	1512 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC048405, AAH48405
RefSeq Size	2172 bp
RefSeq ORF	1514 bp
Locus ID	108013
Gene Summary	RNA-binding protein implicated in the regulation of pre-mRNA alternative splicing. Mediates exon inclusion and/or exclusion in pre-mRNA that are subject to tissue-specific and developmentally regulated alternative splicing. Specifically activates exon 5 inclusion of cardiac isoforms of TNNT2 during heart remodeling at the juvenile to adult transition. Promotes exclusion of both the smooth muscle (SM) and non-muscle (NM) exons in actinin pre-mRNAs. Activates the splicing of MAPT/Tau exon 10. Binds to muscle-specific splicing enhancer (MSE) intronic sites flanking the alternative exon 5 of TNNT2 pre-mRNA (By similarity).[UniProtKB/Swiss-Prot Function]

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