Ranbp3l (BC058706) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	TurboGFP
Synonyms	C130037N17Rik
Vector	pCMV6-AC-GFP
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MG207865 representing BC058706 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCTACAACACAAAGGAAAGACGACAGCCACCTGTTCACCAGTTCGTGCACGCGTCAGCTGCAGGTGC AGGAGGATCGGCAACAGCAAGAAAAATATGTCATTGCTCAGCCAATATTTGTTTTTGAGAAAGGAGAACA CAATTTTAAGAGACCTGCAGAAGACAGCCTGGAAGAAACGGCAGAGCCTGAATTCACTGGTTTCTTGAGA AAGCGTGTAAGGTCTTCATCTGTTACGCTTCATACTACAGATCCTCAGTCTCAAGGAGTGGCAACTTTGT CTCAAACGCGTCTGAGGTCCTCGTCATTCACTGATGTCCCGACCTTCCCTCCATGCAGGCCAGTGAGGAA AAACAATGTTTTTATGACATCACGTCTGCTGCAAAGGAGTGATGACATGAACAACGTAGAGCAAGGACCC CCCATGAGATCTTCTGAACAGGTTCTAAGACCCGCTGTTCTGCAGCCGTCTCAAACTCAGAGTTGTCAGA AAGCAGGCACAACATTTGGGCCCGGTGCATTGAAATCCTACAAAACTAAGGAAAAGGCCGAGCACGAGAT TTCTGAAGTTGGAAGTTCCTCTAGTTTACTAAGTGAAAATTTACCAAATGCCAGAAGCTCAATCCAGCTG TCTACTGATCCATGCATCTCAGAGGCACCATCGGGATGCCAACCAAAGGAAGATAAGTGTTCTTTTACAA GCTGCAGTTCTGACTTCGTTTTTGGAGAAAACATGGTAGAAAGAGTTTTGGGCACTCAAAAACTTACTCA GCCTCCACTTCAAAATCTTTCATATGCCAAGGAAAAAACATTCAAATCTGTTCTGAAATTCCCTAATGCT GTTTCAAATTCAGACTCTATTGAAAACATATCCCTAGTCGAATCAGCTGCTGCTTTTTCCTCCAAACCAT CACAAAAATGCTTGCTTGAGAAGATTGATGTTATAACAGGAGAAGAAACTGAACATAATGTGTTAAAGAT CAACTGCAAGATCTTTGTATTCAACAAAGCCACAGAATCCTGGAGTGAAAGAGGCCAAGGGATTTTGCGA CTGAACGACACGGCCGGCCGAGAGTGTGGAACACTGCAGTCAAGACTAATCATGCGCAACCAAGGCAGTC TGAGGCTGGTCCTCAACAGCAGACTCTGGGCTCAGATGAAGATTCAAAGAGCAAGTCAGAAAAATTTGCG AATAACTGCGACTGATTTAGAAGACGATGGTATAAAAATATTTTTAATTCAGGCTAGTGCCAAAGATACA GGCTTTCTGTATGCAGCAATCCACCACCGTCTGGTCGCGCTTCGAAGCCTCGCTAAGCAGGGTGACGGTG GTCCAGCCGAAAGCCAGTCCGATACAGCCCTCCCTCAGCTCAATGGCGAGAGCTGTGACGAGGATGAGGA TGAGATCGCACAAGTCACTAAAAATGGATCAGATCCTTCAAGGTGGAGCCACAGACAGTCAATTGTCTGT TCA AGCGGACCGACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA Protein Sequence (showhide) >MG207865 representing BC058706 Red=Cloning site Green=Tags(s) MSTTQRKDDSHLFTSSCTRQLQVQEDRQQQEKYVIAQPIFVFEKGEHNFKRPAEDSLEETAEPEFTGFLR KRVRSSSVTLHTTDPQSQGVATLSQTRLRSSSFTDVPTFPPCRPVRKNNVFMTSRLLQRSDDMNNVEQGP PMRSSEQVLRPAVLQPSQTQSCQKAGTTFGPGALKSYKTKEKAEHEISEVGSSSSLLSENLPNARSSIQL STDPCISEAPSGCQPKEDKCSFTSCSSDFVFGENMVERVLGTQKLTQPPLQNLSYAKEKTFKSVLKFPNA VSNSDSIENISLVESAAAFSSKPSQKCLLEKIDVITGEETEHNVLKINCKIFVFNKATESWSERGQGILR LNDTAGRECGTLQSRLIMRNQGSLRLVLNSRLWAQMKIQRASQKNLRITATDLEDDGIKIFLIQASAKDT GFLYAAIHHRLVALRSLAKQGDGGPAESQSDTALPQLNGESCDEDEDEIAQVTKNGSDPSRWSHRQSIVC S SGPTRTRRLE - GFP Tag - V
Restriction Sites	SgfI-RsrII      Cloning Scheme for this gene
ACCN	BC058706
ORF Size	1473 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC058706, AAH58706
RefSeq Size	4128 bp
RefSeq ORF	1475 bp
Locus ID	223332
Gene Summary	Nuclear export factor for BMP-specific SMAD1/5/8 that plays a critical role in terminating BMP signaling and regulating mesenchymal stem cell differentiation by blocking osteoblast differentiation to promote myogenic differention. Directly recognizes dephosphorylated SMAD1/5/8 and mediates their nuclear export in a Ran-dependent manner.[UniProtKB/Swiss-Prot Function]

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