Btbd1 (BC072618) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	TurboGFP
Synonyms	MGC37676
Vector	pCMV6-AC-GFP
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MG207819 representing BC072618 Red=Cloning site Blue=ORF Green=Tags(s) GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGGCGCGCCC ATGGCCTCGCTCGGGTCAGCCGCGGCCGGGGAGCCGGCGACCGGAGCTGAGGCGGAGCCCGGCCCTCCGG CCCCGCCGCCGCCTCCGCCGCCGCCACCGGCTCCCTCGCCGTCCGCTCTCGGGCCGCTGCTGCCCCTGCA GCGCGAGCCGCTGTACAACTGGCAGGCCACCAAGGCGTCGCTCAAGGAGCGCTTCGCCTTCCTCTTCAAC TCGGAGCTGCTGAGCGATGTGCGTTTCGTGCTGGGCAAGGGCCGAGGCGCGGCGGCCGCCGGGGGCCCGC AGCGCATCCCCGCCCACCGCTTCGTGCTGGCGGCCGGCAGCGCGGTCTTCGACGCCATGTTCAACGGCGG CATGGCCACGACGTCGGCCGAGATCGAGCTGCCGGACGTGGAGCCCGCCGCCTTCCTGGCGCTGCTGAGA TTTTTGTATTCAGACGAAGTTCAAATTGGTCCAGAAACCGTTATGACCACTCTTTATACTGCTAAGAAAT ATGCAGTCCCTGCCTTGGAAGCACATTGCGTAGAATTTCTCACCAAACATCTTAGGGCAGATAATGCCTT TATGTTGCTTACTCAGGCGCGGTTGTTCGATGAGCCCCAGCTGGCTAGTCTTTGTCTGGACACCATAGAC AAGAGCACAGTGGATGCAATCAGTGCGGAGGGGTTTACTGATATTGACATAGATACACTCTGTGCAGTTC TAGAAAGAGACACACTGAGTATCCGAGAAAGTCGACTTTTTGGAGCTATTGTACGTTGGGCAGAAGCAGA GTGTCAGAGACAACAACTGGCTGTGACATTTGGAAACAAACAGAAAGTTCTAGGAAAAGCACTTTCCTTA ATCCGCTTCCCACTGATGACAATTGAGGAGTTTGCAGCAGGTCCTGCTCAATCTGGAATTTTGTCAGACC GTGAAGTGGTAAACCTCTTTCTTCATTTTACCGTCAATCCTAAACCCCGAGTAGAGTACATTGATCGGCC ACGATGCTGCCTCAGAGGAAAGGAGTGCTGCATCAACCGATTCCAGCAAGTAGAGAGCCGCTGGGGTTAC AGTGGGACAAGCGATCGCATCAGGTTCACAGTTAACAGAAGAATCTCTGTAGTTGGATTTGGTTTGTATG GATCTATTCACGGGCCCACGGATTATCAAGTGAATATACAGATCATTGAATATGAGAAAAAACAAACCTT GGGACAGAATGATACTGGATTTAGTTGTGATGGGACTGCTAACACATTCAGGGTCATGTTCAAAGAACCT ATAGAGATCCTGCCCAATGTCTGTTACACAGCGTGCGCAACACTCAAGGGCCCAGATTCTCACTATGGCA CAAAAGGACTGAAGAAAGTAGTGCATGAGACCCCTGCTGCAAGCAAGACTGTTTTCCTATTTTTCAGTTC CCCTGGCAATAACAATGGCACATCAATAGAAGATGGACAAATACCAGAAATAATATTTTATACA ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA Protein Sequence (showhide) >MG207819 representing BC072618 Red=Cloning site Green=Tags(s) MASLGSAAAGEPATGAEAEPGPPAPPPPPPPPPAPSPSALGPLLPLQREPLYNWQATKASLKERFAFLFN SELLSDVRFVLGKGRGAAAAGGPQRIPAHRFVLAAGSAVFDAMFNGGMATTSAEIELPDVEPAAFLALLR FLYSDEVQIGPETVMTTLYTAKKYAVPALEAHCVEFLTKHLRADNAFMLLTQARLFDEPQLASLCLDTID KSTVDAISAEGFTDIDIDTLCAVLERDTLSIRESRLFGAIVRWAEAECQRQQLAVTFGNKQKVLGKALSL IRFPLMTIEEFAAGPAQSGILSDREVVNLFLHFTVNPKPRVEYIDRPRCCLRGKECCINRFQQVESRWGY SGTSDRIRFTVNRRISVVGFGLYGSIHGPTDYQVNIQIIEYEKKQTLGQNDTGFSCDGTANTFRVMFKEP IEILPNVCYTACATLKGPDSHYGTKGLKKVVHETPAASKTVFLFFSSPGNNNGTSIEDGQIPEIIFYT TRTRPLE - GFP Tag - V
Restriction Sites	AscI-MluI      Cloning Scheme for this gene
ACCN	BC072618
ORF Size	1464 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC072618, AAH72618
RefSeq Size	3020 bp
RefSeq ORF	1466 bp
Locus ID	83962
Gene Summary	Probable substrate-specific adapter of an E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins (By similarity). Seems to regulate expression levels and/or subnuclear distribution of TOP1, via an unknown mechanism (PubMed:15486563, PubMed:17462629). May play a role in mesenchymal differentiation where it promotes myogenic differentiation and suppresses adipogenesis (PubMed:15486563, PubMed:17462629).[UniProtKB/Swiss-Prot Function]

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