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Mmp12 (NM_008605) Mouse Tagged ORF Clone
CAT＃: MG207567
- TrueORF®
Mmp12 (tGFP-tagged) - Mouse matrix metallopeptidase 12 (Mmp12)
ORF Plasmid: DDK
"NM_008605" in other vectors (4)
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CNY 7,088.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | AV378681; MME; Mmel; MMP1 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG207567 representing NM_008605
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGCTGCACTCTGCTGAAAGGAGTCTGCACAATGAAATTTCTCATGATGATTGTGTTCTTACAGGTAT CTGCCTGTGGGGCTGCTCCCATGAATGACAGTGAATTTGCTGAATGGTACTTGTCAAGATTTTATGATTA TGGAAAGGACAGAATTCCAATGACAAAAACAAAAACCAATAGAAACTTCCTAGAAGAAAAACTCCAGGAA ATGCAGCAGTTCTTTGGGCTAGAAGCAACTGGGCAACTGGACAACTCAACTCTGGCAATAATGCACATCC CTCGATGTGGAGTGCCCGATGTACAGCATCTTAGAGCAGTGCCCCAGAGGTCAAGATGGATGAAGCGGTA CCTCACTTACAGGATCTATAATTACACTCCGGACATGAAGCGTGAGGATGTAGACTACATATTTCAGAAA GCTTTCCAAGTCTGGAGTGATGTGACTCCTCTAAGATTCAGAAAGCTTCATAAAGATGAGGCTGACATTA TGATACTTTTTGCATTTGGAGCTCACGGAGACTTCAACTATTTTGATGGCAAAGGTGGTACACTAGCCCA TGCTTTTTATCCTGGACCTGGTATTCAAGGAGATGCACATTTTGATGAGGCAGAAACGTGGACTAAAAGT TTTCAAGGCACAAACCTCTTCCTTGTTGCTGTTCATGAACTTGGCCATTCCTTGGGGCTGCAGCATTCCA ATAATCCAAAGTCAATAATGGACCCCACCTACAGATACCTTAACCCCAACACATTTCGCCTCTCTGCTGA TGACATACGTAACATTCAGTCCCTCTATGGAGCCCCAGTGAAAACCCCATCCTTGACAAAACCTAGCAGT CCACCATCAACTTTCTGTCACCAAAGCTTGAGTTTTGATGCTGTCACAACAGTGGGAGAGAAAATCTTTT TCTTTAAAGACTGGTTCTTCTGGTGGAAGCTTCCTGGGAGTCCAGCCACCAACATTATTTCTATTTCTTC CATATGGCCAAGCATCCCATCTGGTATTCAAGCTGCTTATGAAATTGAAAGCAGAAATCAACTTTTCCTT TTTAAAGATGAGAAGTACTGGTTAATAAACAACTTAGTACCAGAGCCACACTATCCCAGGAGCATATATT CCCTGGGCTTCTCTGCATCTGTGAAGAAGGTTGATGCAGCTGTCTTTGACCCACTTCGCCAAAAGGTTTA TTTCTTTGTGGATAAACAATACTGGAGGTATGATGTGAGGCAGGAGCTCATGGACCCTGCTTACCCCAAG CTGATTTCCACACACTTCCCAGGAATCAAGCCTAAAATTGATGCAGTCCTCTATTTCAAAAGACACTACT ACATCTTCCAAGGAGCCTATCAATTGGAATATGACCCCCTGTTCCGTCGTGTCACCAAAACATTGAAAAG TACAAGCTGGTTTGGTTGT ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG207567 representing NM_008605
Red=Cloning site Green=Tags(s) MSCTLLKGVCTMKFLMMIVFLQVSACGAAPMNDSEFAEWYLSRFYDYGKDRIPMTKTKTNRNFLEEKLQE MQQFFGLEATGQLDNSTLAIMHIPRCGVPDVQHLRAVPQRSRWMKRYLTYRIYNYTPDMKREDVDYIFQK AFQVWSDVTPLRFRKLHKDEADIMILFAFGAHGDFNYFDGKGGTLAHAFYPGPGIQGDAHFDEAETWTKS FQGTNLFLVAVHELGHSLGLQHSNNPKSIMDPTYRYLNPNTFRLSADDIRNIQSLYGAPVKTPSLTKPSS PPSTFCHQSLSFDAVTTVGEKIFFFKDWFFWWKLPGSPATNIISISSIWPSIPSGIQAAYEIESRNQLFL FKDEKYWLINNLVPEPHYPRSIYSLGFSASVKKVDAAVFDPLRQKVYFFVDKQYWRYDVRQELMDPAYPK LISTHFPGIKPKIDAVLYFKRHYYIFQGAYQLEYDPLFRRVTKTLKSTSWFGC TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_008605 |
| ORF Size | 1419 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_008605.3, NP_032631.3 |
| RefSeq Size | 3607 bp |
| RefSeq ORF | 1422 bp |
| Locus ID | 17381 |
| UniProt ID | P34960 |
| Gene Summary | This gene encodes a member of the matrix metalloproteinase family of extracellular matrix-degrading enzymes that are involved in tissue remodeling, wound repair, progression of atherosclerosis and tumor invasion. The encoded preproprotein undergoes proteolytic processing to generate a mature, zinc-dependent endopeptidase enzyme. Mice lacking the encoded protein have a diminished capacity to degrade extracellular matrix components, do not develop emphysema in response to long-term exposure to cigarette smoke, and exhibit impaired clearance and increased mortality upon bacterial infection. This gene is located in a cluster of other matrix metalloproteinase genes on chromosome 9. Alternate splicing generates multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Feb 2016] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC216411 | Mmp12 (untagged) - Mouse matrix metallopeptidase 12 (Mmp12), (10ug) |
CNY 6,180.00 |
|
| MR207567 | Mmp12 (Myc-DDK-tagged) - Mouse matrix metallopeptidase 12 (Mmp12) |
CNY 5,488.00 |
|
| MR207567L3 | Lenti ORF clone of Mmp12 (Myc-DDK-tagged) - Mouse matrix metallopeptidase 12 (Mmp12) |
CNY 7,888.00 |
|
| MR207567L4 | Lenti ORF clone of Mmp12 (mGFP-tagged) - Mouse matrix metallopeptidase 12 (Mmp12) |
CNY 7,888.00 |
