Eif2s3x (BC063755) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	TurboGFP
Synonyms	AA409828; AA547477; AI314668; Eif-2gx
Vector	pCMV6-AC-GFP
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MG207538 representing BC063755 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCTGGGGGTGAGGGTGGAGTGACTCTCGGTCAGCCCCATCTTTCCCGACAGGATCTCGCCACATTGG ATGTTACCAAGTTGACACCACTTTCACATGAAGTTATCAGCAGACAAGCTACAATTAATATTGGTACAAT TGGTCACGTAGCTCATGGGAAGTCCACTGTGGTGAAGGCTATTTCTGGAGTTCACACTGTCAGATTCAAA AATGAACTCGAAAGGAACATTACCATCAAGCTTGGATATGCTAATGCTAAGATTTATAAACTTGATGACC CAAGTTGTCCTAGACCAGAATGTTACAGATCGTGTGGAAGCAGCACACCAGATGAGTTTCCTACAGACAT TCCGGGGACCAAAGGGAACTTCAAGTTAGTCAGGCATGTTTCCTTTGTTGACTGTCCTGGCCATGATATT TTGATGGCTACGATGCTGAACGGTGCAGCAGTGATGGATGCGGCCCTTCTGTTGATAGCTGGTAACGAAT CATGCCCTCAGCCCCAGACTTCCGAACACCTGGCTGCCATAGAAATCATGAAACTCAAGCATATTTTGAT CTTACAGAATAAAATTGATCTGGTAAAAGAAAGTCAGGCTAAGGAACAATATGAGCAAATCCTCGCATTT GTGCAAGGAACCGTAGCAGAAGGGGCACCAATTATTCCAATTTCTGCTCAACTGAAGTACAATATTGAAG TTGTCTGTGAGTATATAGTGAAGAAAATACCAGTGCCCCCAAGAGACTTTACTTCAGAGCCCCGTCTCAT TGTCATCAGGTCATTTGATGTCAACAAACCTGGCTGTGAAGTCGATGACCTTAAGGGAGGTGTAGCTGGT GGTAGTATCCTAAAAGGAGTGTTAAAGGTAGGCCAGGAGATAGAAGTGAGACCCGGTATTGTCTCCAAAG ACAGTGAAGGAAAACTCATGTGTAAACCAATCTTTTCCAAAATTGTGTCACTTTTTGCGGAACATAATGA TCTTCAATATGCTGCCCCGGGTGGTCTCATTGGAGTTGGAACAAAGATCGACCCCACCTTGTGCCGAGCT GACAGAATGGTGGGACAAGTGCTCGGTGCAGTTGGAGCTTTACCTGAAATATTCACCGAACTGGAGATTT CCTATTTCCTGCTTAGACGTCTTCTGGGTGTACGTACTGAAGGAGACAAGAAAGCAGCAAAGGTCCAAAA GCTGTCTAAGAATGAAGTGCTTATGGTCAACATAGGCTCCCTGTCGACAGGAGGAAGAGTTAGTGCAGTC AAGGCTGATTTGGGCAAAATTGTTTTGACCAATCCCGTGTGCACAGAAGTAGGAGAAAAAATTGCCCTTA GCAGAAGAGTTGAGAAACACTGGCGTTTAATTGGTTGGGGTCAGATAAGAAGAGGAGTGACCATTAAGCC AACTGTAGATGATGAC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA Protein Sequence (showhide) >MG207538 representing BC063755 Red=Cloning site Green=Tags(s) MAGGEGGVTLGQPHLSRQDLATLDVTKLTPLSHEVISRQATINIGTIGHVAHGKSTVVKAISGVHTVRFK NELERNITIKLGYANAKIYKLDDPSCPRPECYRSCGSSTPDEFPTDIPGTKGNFKLVRHVSFVDCPGHDI LMATMLNGAAVMDAALLLIAGNESCPQPQTSEHLAAIEIMKLKHILILQNKIDLVKESQAKEQYEQILAF VQGTVAEGAPIIPISAQLKYNIEVVCEYIVKKIPVPPRDFTSEPRLIVIRSFDVNKPGCEVDDLKGGVAG GSILKGVLKVGQEIEVRPGIVSKDSEGKLMCKPIFSKIVSLFAEHNDLQYAAPGGLIGVGTKIDPTLCRA DRMVGQVLGAVGALPEIFTELEISYFLLRRLLGVRTEGDKKAAKVQKLSKNEVLMVNIGSLSTGGRVSAV KADLGKIVLTNPVCTEVGEKIALSRRVEKHWRLIGWGQIRRGVTIKPTVDDD TRTRPLE - GFP Tag - V
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene
ACCN	BC063755
ORF Size	1416 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC063755, AAH63755
RefSeq Size	3664 bp
RefSeq ORF	1418 bp
Locus ID	26905
Gene Summary	As a subunit of eukaryotic initiation factor 2 (eIF2), involved in the early steps of protein synthesis. In the presence of GTP, eIF2 forms a ternary complex with initiator tRNA Met-tRNAi and then recruits the 40S ribosomal complex, a step that determines the rate of protein translation. This step is followed by mRNA binding to form the 43S pre-initiation complex. Junction of the 60S ribosomal subunit to form the 80S initiation complex is preceded by hydrolysis of the GTP bound to eIF2 and release of an eIF2-GDP binary complex. In order for eIF2 to recycle and catalyze another round of initiation, the GDP bound to eIF2 must exchange with GTP by way of a reaction catalyzed by eIF2B (By similarity). Along with its paralog on chromosome Y, may contribute to spermatogenesis up to the round spermatid stage (PubMed:26823431).[UniProtKB/Swiss-Prot Function]

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