Nasp (BC004693) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	TurboGFP
Synonyms	5033430J04Rik; AI131596; AI317140; D4Ertd767e; Epcs32; Nasp-T
Vector	pCMV6-AC-GFP
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MG207154 representing BC004693 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCACAGAGTCTACAGCCGCTGCTGCCATCGCCGCGGAGCTGGTTTCCGCTGACAAAATTGAAGATG CTCCTGCTCCTTCTACCTCGGCAGATAAAATGGAGAGTCTGGATGTGGATAGTGAAGCTAAGAAACTACT GGGATTAGGACAGAAACATCTGGTGATGGGTGACATTCCAGCAGCTGTTAATGCATTCCAGGAAGCAGCT AGTCTTTTAGGTAAGAAGTATGGAGAAACGGCTAATGAGTGTGGAGAAGCCTTCTTTTTTTATGGGAAGT CACTTCTGGAGTTAGCCAGAATGGAGAATGGAGTGTTGGGAAATGCCTTAGAAGGAGTGCATGTAGAAGA GGAAGAAGGAGAAAAAACAGAAGATGAATCTTTGGTAGAAAATAATGATAATGTAGATGAAACTGAAGGC TCAGAGGAAGAGGACAGAGAAAATGACAAGGCTGAAGAAACACCAAATGAATCAGTTCTTGAAAAAAAGT CTCTTCAAGAAAATGAAGAGGAGGAGATAGGGAATTTGGAACTTGCCTGGGATATGCTGGATTTAGCCAA GATCATTTTTAAGAGGCAAGAAACAAAAGAAGCCCAGCTTTATGCTGCACAAGCCCATCTTAAACTTGGA GAAGTTAGTGTTGAATCTGAGAATTATATCCAAGCTGTGGAGGAGTTTCAGGCTTGCCTAAGCCTGCAAG AACAATATTTGGAAGCTCACGATCGTCTCCTTGCAGAGACTCACTACCAATTAGGTTTGGCCTATGGTTA CAACTCTCAGTATGATGAAGCAGTAGCACAGTTTGGCAAATCTATCGATGTCATTGAAAAGAGAATGGCT GTACTCCATGAGCAGATGAAGGAGGCCGAAGGATCGTTTACTGAATATGAGAAAGAAATTGAGGAGCTGA AAGAACTGCTACCTGAAATTCGAGAGAAGATAGAAGACGCAAAGGAATCTCAGCGGAGTGGGAATGTAGC CGAACTGGCACTGAAAGCTACTCTGGTGGAGAGCTCTACTTCCGGTTTCACTCCTAGTGGAGCTGGTGCC TCAGTATCCATGATTGCCAGTAGAAAACCAACAGATGGCGCTTCCTCATCAAATTGTGTGACTGATATTT CCCATCTTGTCAGAAAGAAGAGGAAACCAGAGGAAGAGAGTCCCCGGAAGGATGATGCAAAGAAAGCTAA ACAAGAGCCAGAGGTGAATGGAGGCAGTGGGGATGCTGTCTCCAGTGGAAAGGAAGTTTCAGAAAACATG GAGGCCGAGGCTGAGAATCAGGCTGAGAGCCAAACAGCAGAGGGGACCGTGGAGTCTGCAGCTACAATTA AAAGCACTGCATGT ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA Protein Sequence (showhide) >MG207154 representing BC004693 Red=Cloning site Green=Tags(s) MATESTAAAAIAAELVSADKIEDAPAPSTSADKMESLDVDSEAKKLLGLGQKHLVMGDIPAAVNAFQEAA SLLGKKYGETANECGEAFFFYGKSLLELARMENGVLGNALEGVHVEEEEGEKTEDESLVENNDNVDETEG SEEEDRENDKAEETPNESVLEKKSLQENEEEEIGNLELAWDMLDLAKIIFKRQETKEAQLYAAQAHLKLG EVSVESENYIQAVEEFQACLSLQEQYLEAHDRLLAETHYQLGLAYGYNSQYDEAVAQFGKSIDVIEKRMA VLHEQMKEAEGSFTEYEKEIEELKELLPEIREKIEDAKESQRSGNVAELALKATLVESSTSGFTPSGAGA SVSMIASRKPTDGASSSNCVTDISHLVRKKRKPEEESPRKDDAKKAKQEPEVNGGSGDAVSSGKEVSENM EAEAENQAESQTAEGTVESAATIKSTAC TRTRPLE - GFP Tag - V
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	BC004693
ORF Size	1346 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	BC004693, AAH04693
RefSeq Size	1548 bp
RefSeq ORF	1346 bp
Locus ID	50927
Gene Summary	Required for DNA replication, normal cell cycle progression and cell proliferation. Forms a cytoplasmic complex with HSP90 and linker H1 histones and stimulates HSP90 ATPase activity. NASP and H1 histone are subsequently released from the complex and translocate to the nucleus where the histone is released for binding to DNA.[UniProtKB/Swiss-Prot Function]

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